Activity Test of Suji Leaf Extract (Dracaena angustifolia Roxb.) on in vitro cholesterol lowering

DOI: https://doi.org/10.14710/jksa.21.2.54-58
Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Article Metrics: (Click on the Metric tab below to see the detail)

Cholesterol is a natural substance with physical characteristic similar to fat but has a steroidal group. The body requires cholesterol in normal amount; however, it will harm the body in excess amount. High cholesterol levels in the blood are dangerous because of the precipitation of cholesterol and other fatty substances resulting in atherosclerosis. Suji leaf (Dracaena angustifolia Roxb.) used as a natural dye has a high flavonoid content that is inferred to have cholesterol-lowering activity. This study aims to test the in vitro activity of suji leaf (Dracaena angustifolia Roxb.) extract in decreasing cholesterol level with various concentrations and to find the effective concentration (EC50). The method of extraction used was remaceration method with 70% ethanol solvent. Analysis of cholesterol-lowering activity was done by Lieberman-Burchard method by making variation of ethanol extract 400 ppm, 500 ppm, 600 ppm, 700 ppm, and 800 ppm. The results showed the percentage of cholesterol-lowering activity by 33.62%, 36.15%, 46.61%, 56.39% and 64.05% respectively. Value of EC50 activity of suji leaf extract is 632.50 ppm.

Keywords

Cholesterol, Flavonoids, Suji Leaves (Dracaena angustifolia Roxb.), Lieberman-Burchard, Remaseration

  1. Devina Ingrid Anggraini  Orcid Sinta
    Sekolah Tinggi Ilmu Kesehatan (STIKES) Nasional Surakarta, Program Studi D3 Farmasi, Sekolah Tinggi Ilmu Kesehatan (STIKES) Nasional Surakarta Surakarta, Indonesia
  2. Lily Fathrah Nabillah 
    Fakultas Kesehatan, Universitas MH Thamrin Jakarta, Indonesia
  1. Sri Nilawati, Diah Krisnatuti, B. Mahendra, Oei Gin Djing, Care Yourself. kolesterol, Penerbar Plus+, 2008.
  2. A. Ghanaim Fasya, Potensi Antikanker dan Antioksidan serta Identifikasi Isolat Steroid Mikroalga Chlorella sp, in, UIN Maulana Malik Ibrahim, Malang, 2015, pp. 86.
  3. Tan Hoan Tjay, Kirana Rahardja, Obat-Obat Penting: Khasiat, Penggunaan dan Efek-Efek Sampingnya, 6th ed., Elex Media Komputindo, Jakarta, 2007.
  4. Ulfah Nurrahmani, Stop Kolesterol Tinggi, Familia, 2012.
  5. Yudi Garnadi, Hidup Nyaman dengan Hiperkolesterol, AgroMedia Pustaka, Jakarta, 2012.
  6. Gagas Ulung, Sehat Alami dengan Herbal: 250 Tanaman Berkhasiat Obat, Gramedia Pustaka Utama Jakarta, 2014.
  7. P. C. Ochani, P. D'Mello, Antioxidant and antihyperlipidemic activity of Hibiscus sabdariffa Linn. leaves and calyces extracts in rats, Indian journal of experimental biology, 47, 4, (2009) 276-282
  8. R. Nalole, M. N. Djide, E. Wahyudin, A. I. Makhmud, Uji In Vitro Penurunan Kadar Kolesterol oleh Sari Kedelai Hitam (Glycine max Merr), Majalah Farmasi dan Farmakologi, 13, 1, (2009) 17-20
  9. K. R. Markham, Cara Mengidentifikasi Flavonoid, K. Padmawinata, ITB, Bandung, 1988.
  10. Soni Himesh, Sharma Sarvesh, Patel Sita Sharan, K. Mishra, A. K. Singhai, Preliminary Phytochemical Screening and HPLC Analysis of Flavonoid From Methanolic Extract of Leaves of Annona squamosa, International Research Journal of Pharmacy, 2, 5, (2011) 242-246
  11. Republik Indonesia. Departemen Kesehatan, Sediaan galenik, Departemen Kesehatan, Republik Indonesia, 1986.
  12. R.C. Rowe, P.J. Sheskey, S.C. Owen, Handbook of Pharmaceutical Excipients, Pharmaceutical Press, 2006.
  13. Muhammad Saiful Amin, Studi in vitro ; Efek Antikolesterol dari Ekstrak Metanol Buah Parijoto (Medinilla speciosa Blume) Terhadap Kolesterol Total, Program Studi Farmasi, UIN Syarif Hidayatullah, Jakarta
  14. James M. Orten, Otto Wilhelm Neuhaus, Human Biochemistry, Mosby, 1982.
  15. D. Setyawan Purwo Handoko, Kinetika Hidrolisis Maltosa pada Variasi Suhu dan Jenis Asam sebagai Katalis, SIGMA: Jurnal Sains dan Teknologi, 9, 1, (2006) 9
  16. Kurnia Agustini, Shanti Marlina, Azizahwati, Pengaruh Lama Pemberian Formula Ekstrak Buah Labu Siam (Sechium edule) terhadap Penurunan Kadar Kolesterol Total dan Trigliserida Tikus Putih Jantan, Jurnal Bahan Alam Indonesia, 6, 2, (2007) 60-64
  17. Muharram Priatna, Ade Irma Sartika, Ria Ambaryani, Uji Banding Aktivitas Antikolesterol Ekstrak Etanol Buah Pepino (Solanum muricatum. Ait) dan Buah Strawberry (Fragaria X ananassa duchesne) pada Tikus Putih Jantan, Jurnal Kesehatan Bakti Tunas Husada: Jurnal Ilmu-Ilmu Keperawatan, Analis Kesehatan dan Farmasi, 13, 1, (2015) 165-172
  18. R. W. Burke, B. I. Diamondstone, R. A. Velapoldi, O. Menis, Mechanisms of the Liebermann-Burchard and Zak color reactions for cholesterol, Clinical Chemistry, 20, 7, (1974) 794-781
  19. Effendy, Teori VSEPR: kepolaran, dan gaya antarmolekul, Bayumedia, Malang, 2006.