DOI: https://doi.org/10.14710/jksa.24.4.114-119

Potential Antibacterial Activity of Bioactive β-sitosterol from Root Bark of Rhizophora apiculata from Lampung Coastal

1Chemistry Study Program, Science Department, Institute Technology of Sumatera, Bandar Lampung, Indonesia

2Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Lampung, Bandar Lampung, Indonesia

3Center for Research and Innovation of Biological Materials and Natural Materials, Institute Technology of Sumatera, Bandar Lampung, Indonesia

Received: 2 Feb 2021; Revised: 26 Mar 2021; Accepted: 27 Mar 2021; Published: 30 Apr 2021.
Open Access Copyright 2021 Jurnal Kimia Sains dan Aplikasi under http://creativecommons.org/licenses/by-sa/4.0.

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Abstract
β-sitosterol is an essential bioactive phytosterol naturally present in plant cell membranes. It has a coincident structure with animal cholesterol. This investigation reported isolation, structure analysis, and an antimicrobial assay of β-sitosterol from the root bark of Bakau Minyak (Rhizophora apiculata) from Lampung coastal. The isolation of β-sitosterol was carried out through maceration using methanol, separation by vacuum liquid chromatography (VLC), and purification by column chromatography (CC) using ethyl acetate/n-hexane (2:8) as eluent. The structure of β-sitosterol was determined using spectroscopic analysis (UV-Vis, FT-IR, 13C-NMR, 1H-NMR, DEPT, and GC-MS). The pure β-sitosterol has 107.4 mg of white needle crystalline compound, the compound melting point about 140.7-141.2oC, the molecular mass confirmed by m/z 414, and UV absorption detected at λ 203.9 nm. The β-sitosterol antimicrobial bioactivity assay has shown potential activity to be developed as a lead compound against E. coli.
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Keywords: Mangrove; β-sitosterol; antibacterial; Lampung coastal

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Section: Research Articles
Language : EN
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