1Department of Chemistry, Faculty of Mathematics and Natural Science, IPB University, Bogor, Indonesia
2Tropical Biopharmaca Research Center, IPB University,Bogor, Indonesia
3Department of Horticulture, Faculty of Agriculture, University of Khartoum, Khartoum, Sudan
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@article{JKSA46119, author = {Irmanida Batubara and Arnold Siahaan and Eti Rohaeti and Ali Muddathir}, title = {Antibacterial Activity of Jojoba Seed residue and Its Possible Active Compound}, journal = {Jurnal Kimia Sains dan Aplikasi}, volume = {25}, number = {6}, year = {2022}, keywords = {Simmondsia chinensis; Embelin; Staphylococcus aureus; Escherichia coli}, abstract = {Jojoba ( Simmondsia chinensis ) (Link) C.K. is a shrub plant widely used in cosmetics, especially jojoba oil. The residue will remain when producing jojoba oil and become waste. This study aimed to determine the antibacterial activity of Jojoba seed residue (JSR) and its possible active antibacterial compounds. JSR was collected from Sudan and extracted by maceration with n -hexane, ethyl acetate, and 70% ethanol. The antibacterial activity was determined with the microdilution method against Staphylococcus aureus ( S. aureus ) and Escherichia coli ( E. coli ). The 70% ethanol extract showed the value of MIC and MBC against E. coli , which was 7.8 mg/ml; meanwhile, against S. aureus was 3.9 mg/mL and 7.8 mg/mL, respectively. Fractionation of 70% ethanol extract using silica gel column chromatography with gradient elution produced ten fractions. Fraction 3 showed the MIC and MBC values in E. coli which were 3.1 mg/mL and 12 mg/ml, and in S. aureus , which were 3.1 mg/mL and 6.2 mg/mL. The fractionation continued to Fraction 3 using preparative thin layer chromatography to collect subfraction 3.2 at an Rf value of 0.76, actively based on contact autobiography against E. coli and S. aureus. Embelin was detected in Subfraction 3.2 using liquid chromatography-mass spectrometry (UHPLC-Q-Orbitrap HRMS) and suggested as the active antibacterial component in JSR.}, issn = {2597-9914}, pages = {218--223} doi = {10.14710/jksa.25.6.218-223}, url = {https://ejournal.undip.ac.id/index.php/ksa/article/view/46119} }
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