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Optimization of Cellulase Production by Aspergillus niger InaCC F506 in Solid-State Fermentation of Tofu Dreg

Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University, Jl. Prof. Soedarto, SH., Tembalang, Semarang, Indonesia

Received: 18 Sep 2022; Revised: 19 Nov 2022; Accepted: 12 Dec 2022; Published: 31 Dec 2022.
Open Access Copyright 2022 Jurnal Kimia Sains dan Aplikasi under http://creativecommons.org/licenses/by-sa/4.0.

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Abstract
Indonesia has a growing demand for cellulase enzymes; however, 99% of the enzymes are imported from other countries. Aspergillus niger is well recognized for using the widely accessible tofu by-product, often known as tofu dreg, as a growth medium for synthesizing cellulase enzymes. This study aims to optimize the production of cellulase enzymes by Aspergillus niger InaCC F506 using tofu dregs as a substrate through the Solid-State Fermentation (SSF) method by varying the additives. The results showed that the E fermentation system with the composition of urea 0.5%; CMC 0.5%; KH2PO4 0.2%; MgSO4.7H2O 0.2% produced the highest cellulase enzymes from the tofu dregs substrate. The highest cellulase enzyme activity was at a fraction of ammonium sulfate saturation level of 40-60%. The optimum condition of enzyme activity was observed at pH 5 with an activity of 33 x 10-4 Units/mg protein and at 30℃ with an activity of 31 x 10-4 Units/mg protein.
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Keywords: cellulase enzymes; Aspergillus niger InaCC; tofu dregs; solid-state fermentation
Funding: Universitas Diponegoro

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