Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University, Jl. Prof. Soedarto, SH., Tembalang, Semarang, Indonesia
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@article{JKSA52521, author = {Talisia Kresna Shih and Agustina L. N. Aminin and Nies Suci Mulyani}, title = {Optimization of Cellulase Production by Aspergillus niger InaCC F506 in Solid-State Fermentation of Tofu Dreg}, journal = {Jurnal Kimia Sains dan Aplikasi}, volume = {25}, number = {11}, year = {2022}, keywords = {cellulase enzymes; Aspergillus niger InaCC; tofu dregs; solid-state fermentation}, abstract = {Indonesia has a growing demand for cellulase enzymes; however, 99% of the enzymes are imported from other countries. Aspergillus niger is well recognized for using the widely accessible tofu by-product, often known as tofu dreg, as a growth medium for synthesizing cellulase enzymes . This study aims to optimize the production of cellulase enzymes by Aspergillus niger InaCC F506 using tofu dregs as a substrate through the Solid-State Fermentation (SSF) method by varying the additives. The results showed that the E fermentation system with the composition of urea 0.5%; CMC 0.5%; KH 2 PO 4 0.2%; MgSO 4 .7H 2 O 0.2% produced the highest cellulase enzymes from the tofu dregs substrate. The highest cellulase enzyme activity was at a fraction of ammonium sulfate saturation level of 40-60%. The optimum condition of enzyme activity was observed at pH 5 with an activity of 33 x 10 -4 Units/mg protein and at 30℃ with an activity of 31 x 10 -4 Units/mg protein.}, issn = {2597-9914}, pages = {419--426} doi = {10.14710/jksa.25.11.419-426}, url = {https://ejournal.undip.ac.id/index.php/ksa/article/view/52521} }
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