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Isolation and Antioxidant Activity of Flavonoid Compound in Ethanolic Extract of Celery Leaves (Apium graveolens L.)

Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University, Jl. Prof. Soedarto, SH., Tembalang, Semarang 50271, Indonesia

Received: 26 Sep 2022; Revised: 22 Nov 2022; Accepted: 18 Dec 2022; Published: 31 Dec 2022.
Open Access Copyright 2022 Jurnal Kimia Sains dan Aplikasi under http://creativecommons.org/licenses/by-sa/4.0.

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Abstract
Celery (Apium graveolens L.) is a plant that belongs to the Apiaceae family and is widely used as a medical plant for low blood pressure, heart tonic, and to prevent cardiovascular disease. This study aims to obtain flavonoid compounds, identify, and test the antioxidant activity of flavonoid compounds and crude extracts from celery leaves. The research procedures consisted of four steps, the first of which was a preliminary test. The second step involved isolating and separating flavonoid components by vacuum liquid chromatography, gravitational column chromatography, and preparative thin layer chromatography. The third step was to identify flavonoid compounds using reagent shift, FTIR, and LCMS/MS. And finally, antioxidant activity was evaluated using the DPPH method. The preliminary test result showed that the ethanolic extract of leaves and stems had a total flavonoid content of 13.99 and 2.46 mg QE/g of dry weight. Both dry leaves and crude extract of celery leaves contained alkaloids, saponin, flavonoid, tannin, quinone, and steroid/triterpenoid, as determined by phytochemical screening. Isolation and separation of flavonoids yielded A2.I and A2.II isolates, with respective weights of 8 mg and 14 mg. Identification of flavonoid compounds using reagent shift showed that two isolates have the basic structure of the flavone group. A2.I isolate had OH groups at 4’, 5, and 7, while A2.II isolate had OH groups at 3’, 4’, 5, and 7. The FTIR analysis revealed that both compounds contain functional groups, including O-H, C=O, C=C aromatic, C-O ether, C-O alcohol, and C-H aromatic ring. According to LCMS/MS analysis, the molecular weights of A2.I and A2.II were 270 g/mol and 286 g/mol, respectively. All of the identification methods for isolates showed that A2.I was apigenin and A2.II was luteolin. Antioxidant activity by DPPH method for a viscous extract of celery leaves, A2.I, and A2.II were 775.41, 288.95, and 184.35 µg/mL, respectively.
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Keywords: Isolation; celery leaves; flavonoid; apigenin; luteolin; antioxidant
Funding: Universitas Diponegoro

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  1. Wesam Kooti, Nahid Daraei, A review of the antioxidant activity of celery (Apium graveolens L), Journal of Evidence-Based Integrative Medicine, 22, 4, (2017), 1029-1034 https://doi.org/10.1177/2156587217717415
  2. H. B. Sowbhagya, P. Srinivas, N. J. F. C. Krishnamurthy, Effect of enzymes on extraction of volatiles from celery seeds, Food Chemistry, 120, 1, (2010), 230-234 https://doi.org/10.1016/j.foodchem.2009.10.013
  3. Cheryl A. Lans, Ethnomedicines used in Trinidad and Tobago for urinary problems and diabetes mellitus, Journal of Ethnobiology and Ethnomedicine, 2, 45, (2006), 1-11 https://doi.org/10.1186/1746-4269-2-45
  4. Rafikali A. Momin, Muraleedharan G. Nair, Mosquitocidal, nematicidal, and antifungal compounds from Apium graveolens L. seeds, Journal of Agricultural and Food Chemistry, 49, 1, (2001), 142-145 https://doi.org/10.1021/jf001052a
  5. T. Mencherini, A. Cau, G. Bianco, R. Della Loggia, R. P. Aquino, G. Autore, An extract of Apium graveolens var. dulce leaves: structure of the major constituent, apiin, and its anti-inflammatory properties, Journal of Pharmacy and Pharmacology, 59, 6, (2010), 891 897 https://doi.org/10.1211/jpp.59.6.0016
  6. Bahman Nickavar, Mohammad Kamalinejad, Hamidreza Izadpanah, In vitro free radical scavenging activity of five Salvia species, Pakistan Journal of Pharmaceutical Sciences, 20, 4, (2007), 291-294
  7. Christian Zidorn, Karin Jöhrer, Markus Ganzera, Birthe Schubert, Elisabeth Maria Sigmund, Judith Mader, Richard Greil, Ernst P. Ellmerer, Hermann Stuppner, Polyacetylenes from the Apiaceae vegetables carrot, celery, fennel, parsley, and parsnip and their cytotoxic activities, Journal of Agricultural and Food Chemistry, 53, 7, (2005), 2518-2523 https://doi.org/10.1021/jf048041s
  8. Shawn M. Somerset, Lidwine Johannot, Dietary flavonoid sources in Australian adults, Nutrition and Cancer, 60, 4, (2008), 442-449 https://doi.org/10.1080/01635580802143836
  9. Surangi H. Thilakarathna, H. P. Vasantha Rupasinghe, Flavonoid bioavailability and attempts for bioavailability enhancement, Nutrients, 5, 9, (2013), 3367-3387 https://doi.org/10.3390/nu5093367
  10. Wesam Kooti, Sara Ali-Akbari, Majid Asadi-Samani, Hosna Ghadery, Damoon Ashtary-Larky, A review on medicinal plant of Apium graveolens, Advanced Herbal Medicine, 1, 1, (2014), 48-59
  11. Peng Li, Jia Jia, Daihui Zhang, Jingli Xie, Xueshu Xu, Dongzhi Wei, In vitro and in vivo antioxidant activities of a flavonoid isolated from celery (Apium graveolens L. var. dulce), Food & Function, 5, 1, (2014), 50-56 https://doi.org/10.1039/C3FO60273G
  12. D. T. Berashvili, M. D. Alaniya, A. D. Bakuridze, L. N. Gvazava, G. Balansard, R. Elias, Apigenin Glucuronide from Perilla nankinensis Leaves, Chemistry of Natural Compounds, 41, 1, (2005), 97-98 https://doi.org/10.1007/s10600-005-0086-y
  13. Chun-Nan Lin, Chai-Ming Lu, Pao-Lin Huang, Flavonoids from Artocarpus heterophyllus, Phytochemistry, 39, 6, (1995), 1447-1451 https://doi.org/10.1016/0031-9422(95)00135-T
  14. Aliefman Hakim, A. Wahab Jufri, Jamaluddin, Supriadi, Putri Ayu Mutmainnah, Understanding the Uniqueness of Artocarpus Flavonoids: Isolation and Structure Elucidation of Cycloartocarpin from the Roots of Artocarpus altilis, Journal of Chemical Education, 97, 11, (2020), 4133-4136 https://doi.org/10.1021/acs.jchemed.0c00199
  15. Nabaa Mohammed Ibrahem, Enas Jawad Khadum, Shihab Hattab Mutlag, Isolation of Catchin and Epigallocatchin From Iraqi Rhus coriaria By Preparative High-Performance Liquid Chromatography (PHPLC), Iraqi Journal of Pharmaceutical Sciences, 31, 2, (2022), 271-282 https://doi.org/10.31351/vol31iss2pp271-282
  16. Goran Baranović, Suzana Šegota, Infrared spectroscopy of flavones and flavonols. Reexamination of the hydroxyl and carbonyl vibrations in relation to the interactions of flavonoids with membrane lipids, Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 192, (2018), 473-486 https://doi.org/10.1016/j.saa.2017.11.057
  17. Xiaoqian Yin, Anmin Huang, Shifeng Zhang, Ru Liu, Fang Ma, Identification of Three Dalbergia Species Based on Differences in Extractive Components, Molecules, 23, 9, (2018), 2163 https://doi.org/10.3390/molecules23092163
  18. Peter J. Taormina, Brendan A. Niemira, Larry R. Beuchat, Inhibitory activity of honey against foodborne pathogens as influenced by the presence of hydrogen peroxide and level of antioxidant power, International Journal of Food Microbiology, 69, 3, (2001), 217-225 https://doi.org/10.1016/S0168-1605(01)00505-0

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