skip to main content

Optimization of Guava Leaf Extract Loading in Chitosan-Based Nanoencapsulation for Oxidation Inhibition

Department of Physics, Universitas Diponegoro, Jl. Prof. Sudarto, SH, Tembalang, Semarang 50275, Indonesia

Received: 30 Mar 2026; Revised: 27 May 2026; Accepted: 28 May 2026; Published: 15 Jun 2026.
Open Access Copyright 2026 Jurnal Kimia Sains dan Aplikasi under http://creativecommons.org/licenses/by-sa/4.0.

Citation Format:
Graphical Abstract proses esktrak dan enkapsulasi daun jambu biji sebagai penghambat oksidasi pirit
Abstract

Guava leaves contain various active compounds, including tannins, phenolics, and flavonoids, with potential as antioxidants, antimicrobials, and anti-inflammatory agents. Guava leaf extract has been used in various fields, including manufacturing, as a corrosion inhibitor. Nanoencapsulation is a method for maintaining the stability and durability of guava leaf extract under various environmental conditions, including changes in pH and temperature, as well as chemical degradation. In this study, guava leaf extract was obtained by ultrasonication. Nanoencapsulation of guava leaf extract at varying concentrations was performed using the ionic gelation method with the natural polymer chitosan and the cross-linking agent STPP. Sample characteristics were identified using X-ray Fluorescence Spectroscopy, Fourier Transform Infrared Spectroscopy, Particle Size Analysis, and Inductively Coupled Plasma–Optical Emission Spectroscopy. The optimal concentration of guava leaf extract in this encapsulation study, 2000–3500 ppm, resulted in particle sizes of 315–390 nm and a polydispersity index below 0.5, indicating well-dispersed particles. The smaller, more dispersed particles will increase the electrostatic bonding between particles, thereby strengthening the interactions involved in the formation of the complex layer. This layer reduces pyrite oxidation by decreasing the release of sulfur and iron into the leaching environment. Nanoencapsulation of guava leaf extract using the ionic gelation method with chitosan is quite effective.

Keywords: Nanoencapsulation; Guava Leaves; Ionic Gelation; Chitosan; Inhibition
Funding: Lembaga Pengelola Dana Penelitian-LPDP

Article Metrics:

  1. Anjali Sahal, Siddhant Chaudhary, Afzal Hussain, Shubhangi Arora, Ankita Dobhal, Waseem Ahmad, Vinod Kumar, Sanjay Kumar, A comprehensive review on the nutritional composition, bioactive potential, encapsulation techniques, and food system applications of guava (Psidium guajava L.) leaves, Grain & Oil Science and Technology, 8, 1, (2025), 64-74 http://doi.org/10.1016/j.gaost.2024.12.003
  2. Xinfeng Zou, Haiyang Liu, A review of meroterpenoids and of their bioactivity from guava (Psidium guajava L.), Journal of Future Foods, 3, 2, (2023), 142-154 http://doi.org/10.1016/j.jfutfo.2022.12.005
  3. Risa Dwi Risa, Dia Septiani, Nabilla Faoziyyah, Meisya Dwi Ananda, Potential Bioactivity of Guava Leaf Extract (Psidium guajava L.) as a Natural Therapeutic Agent: A Review, Jurnal Pijar MIPA, 20, 5, (2025), 976-982 http://doi.org/10.29303/jpm.v20i5.8080
  4. A. Ngatin, A. F. Wulandari, A. D. Saffanah, D. R. Suminar, S. Setyaningrum, Pemanfaatan Ekstrak Daun Jambu Biji Sebagai Inhibitur Korosi Baja Paduan dalam Medium Larutan NaCl, Fluida, 15, 2, (2022), 113-120 http://doi.org/10.35313/fluida.v15i2.3923
  5. Meigy Nelce Mailoa, Meta Mahendradatta, Amran Laga, Natsir Djide, Tannin Extract of Guava Leaves (Psidium guajava L) Variation with Concentration Organic Solvents, International Journal of Scientific & Technology Research, 2, 9, (2013), 106-110
  6. Md. Abul Hashem, Md. Shahriar Shahadat, Jannatul Nime Tabassum, Md. Mukimujjaman Miem, Modinatul Maoya, Extraction of tannin from Abrus precetorius seed in leather processing: An eco-friendly approach, Green Technologies and Sustainability, 3, 3, (2025), 100216 http://doi.org/10.1016/j.grets.2025.100216
  7. Helda Niawanti, Fitri Yani, Mu’min Herman, Husnul Rafliansyah, Ekstraksi Tanin Dari Daun Psidium Guajava Menggunakan Metode Soxhlet, DISTILAT: Jurnal Teknologi Separasi, 7, 2, (2021), 353-359 https://doi.org/10.33795/distilat.v7i2.226
  8. Deniz Döner, Filiz Icier, Exergoeconomic analysis of ultrasound-assisted extraction of tannins from acorn fruit, Journal of Food Engineering, 367, (2024), 111851 https://doi.org/10.1016/j.jfoodeng.2023.111851
  9. Cigem Kilicarislan, Hasan Ozgunay, Ultrasound Extraction of Valonea Tannin and Its Effects on Extraction Yield, Journal of the American Leather Chemists Association, 107, 11, (2012), 394-403
  10. Mohamed Rehan, Omar A. Ahmed-Farid, Shaimaa R. Ibrahim, Aliaa Ali Hassan, Areeg M. Abdelrazek, Nagwa I. M. Khafaga, Tawfik A. Khattab, Green and Sustainable Encapsulation of Guava Leaf Extracts (Psidium guajava L.) into Alginate/Starch Microcapsules for Multifunctional Finish over Cotton Gauze, ACS Sustainable Chemistry & Engineering, 7, 22, (2019), 18612–18623 http://doi.org/10.1021/acssuschemeng.9b04952
  11. Eman S. El-Ashaal, Hisham A. Elshoky, Nayera M. El-Sayed, Ebtehal A. El-Kholany, Chitosan and carboxymethyl chitosan nanocarriers enhance mango seed extract stability and antimicrobial activity to improve strawberry postharvest quality, Scientific Reports, 15, (2025), 31384 http://doi.org/10.1038/s41598-025-16756-1
  12. Sandra Aulia Mardikasari, Suryani Suryani, Nur Illiyyin Akib, Rezki Indahyani, Mikroenkapsulasi Asam Mefenamat Menggunakan Polimer Kitosan dan Natrium Alginat dengan Metode Gelasi Ionik, Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) (e-Journal), 6, 2, (2020), 192-203 http://doi.org/10.22487/j24428744.2020.v6.i2.14589
  13. Natalia Cristina Silva, Chloe Chevigny, Sandra Domenek, Giana Almeida, Odílio Benedito Garrido Assis, Milena Martelli-Tosi, Nanoencapsulation of active compounds in chitosan by ionic gelation: Physicochemical, active properties and application in packaging, Food Chemistry, 463, (2025), 141129 https://doi.org/10.1016/j.foodchem.2024.141129
  14. Foram H. Vaghela, Tejal D. Bhatt, Kanji D. Kachhot, Chirag H. Dhamal, Vijay R. Ram, Hitendra S. Joshi, Comparative Energy Dispersive X-Ray Fluorescence Analysis of Mangifera Indica L. Leaves in the Locality of Kachchh and Saurashtra, Progress in Chemical and Biochemical Research, 5, 3, (2022), 254-261 http://doi.org/10.22034/pcbr.2022.349997.1227
  15. Wenrong Chen, Zhenli L. He, Xiao E. Yang, Suren Mishra, Peter J. Stoffella, Chlorine nutrition of higher plants: Progress and perspectives, Journal of Plant Nutrition, 33, 7, (2010), 943-952 http://doi.org/10.1080/01904160903242417
  16. Fahad Khan, Abu Bakar Siddique, Sergey Shabala, Meixue Zhou, Chenchen Zhao, Phosphorus Plays Key Roles in Regulating Plants’ Physiological Responses to Abiotic Stresses, Plants, 12, 15, (2023), 2861 http://doi.org/10.3390/plants12152861
  17. Om Prakash Narayan, Paras Kumar, Bindu Yadav, Meenakshi Dua, Atul Kumar Johri, Sulfur nutrition and its role in plant growth and development, Plant Signaling & Behavior, 18, 1, (2022), 2030082 http://doi.org/10.1080/15592324.2022.2030082
  18. Nindya Wulan Sari, Miskah Yumna Fajri, Anjas Wilapangga, Analisis Fitokimia Dan Gugus Fungsi Dari Ekstrak Etanol Pisang Goroho Merah (Musa acuminate (L)), Indonesian Journal of Biotechnology and Biodiversity, 2, 1, (2018), 30-34
  19. Gustavo Cabrera-Barjas, Nicole Butto-Miranda, Aleksandra Nesic, Mauricio Moncada-Basualto, Rodrigo Segura, Gastón Bravo-Arrepol, Danilo Escobar-Avello, Arash Moeini, Sebastian Riquelme, Andrónico Neira-Carrillo, Condensed tannins from Pinus radiata bark: Extraction and their nanoparticles preparation in water by green method, International Journal of Biological Macromolecules, 278, (2024), http://doi.org/10.1016/j.ijbiomac.2024.134598
  20. Linxin Guo, Taotao Qiang, Yvrui Yang, Ying He, Yi Dou, Zhanpeng Zhang, Tongyue Wu, Heping Wang, Extraction and structural characterization of hydrolyzable tannins from Coriaria nepalensis leaves, Industrial Crops and Products, 215, (2024), 118646 https://doi.org/10.1016/j.indcrop.2024.118646
  21. Joana R. Costa, Miguel Xavier, Isabel R. Amado, Catarina Gonçalves, Pedro M. Castro, Renata V. Tonon, Lourdes M. C. Cabral, Lorenzo Pastrana, Manuela E. Pintado, Polymeric nanoparticles as oral delivery systems for a grape pomace extract towards the improvement of biological activities, Materials Science and Engineering: C, 119, (2021), 111551 http://doi.org/10.1016/j.msec.2020.111551
  22. Dian Ayumi, Sumaiyah Sumaiyah, Masfria Masfria, Pembuatan dan Karaterisasi Nanopartikel Ekstrak Etanol Daun Ekor Naga (Rhaphidophora pinnata (L.f.) Schott) Menggunakan Metode Gelasi Ionik, Talenta Conference Series: Tropical Medicine (TM), 2018 https://doi.org/10.32734/tm.v1i3.257
  23. Dessy Kurniasari, Sri Atun, Pembuatan dan Karakterisasi Nanopartikel Ekstrak Etanol Temu Kunci (Boesenbergia pandurata) pada Berbagai Variasi Komposisi Kitosan, Jurnal Sains Dasar, 6, 1, (2017), 31-35 https://doi.org/10.21831/jsd.v6i1.13610

Last update:

No citation recorded.

Last update: 2026-06-19 09:20:41

No citation recorded.