Green-Synthesised Silver Nanoparticle–Loaded Sodium Alginate Beads Using Asplenium nidus for Potential Antibacterial and Hg²⁺ Ion Sensing Applications

Doni Notriawan; Eka Angasa; Reza Pertiwi; Zavira Nurjali; Meiliana Ngasti Wati

doi:10.14710/jksa.29.8.559-567

DOI: https://doi.org/10.14710/jksa.29.8.559-567

Green-Synthesised Silver Nanoparticle–Loaded Sodium Alginate Beads Using Asplenium nidus for Potential Antibacterial and Hg²⁺ Ion Sensing Applications

Doni Notriawan¹ , Eka Angasa²

, Reza Pertiwi³

, Zavira Nurjali¹, Meiliana Ngasti Wati¹

¹Science Laboratory, Faculty of Mathematics and Natural Sciences, Universitas Bengkulu, Jalan WR. Supratman, Kendang Limun, Kota Bengkulu 38371, Indonesia

²Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Bengkulu, Jalan WR. Supratman, Kandang Limun, Kota Bengkulu 38371, Indonesia

³Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Universitas Bengkulu, Jalan WR. Supratman, Kandang Limun, Kota Bengkulu 38371, Indonesia

Received: 5 Jun 2026; Revised: 31 Aug 2026; Accepted: 22 Sep 2026; Published: 30 Sep 2026.

BibTex Citation Data :

@article{JKSA85255,
    author = {Doni Notriawan and Eka Angasa and Reza Pertiwi and Zavira Nurjali and Meiliana Ngasti Wati},
    title = {Green-Synthesised Silver Nanoparticle–Loaded Sodium Alginate Beads Using Asplenium nidus for Potential Antibacterial and Hg²⁺ Ion Sensing Applications},
    journal = {Jurnal Kimia Sains dan Aplikasi},
  volume = {29},
    number = {8},
    year = {2026},
    keywords = {Asplenium nidus; Silver Nanoparticles; Nanocomposite; Antibacterial},
    abstract = { Silver nanoparticles (AgNPs) have attracted considerable interest due to their antibacterial properties and potential for metal-ion sensing. This study aimed to develop sodium alginate/silver nanoparticle (SA/AgNPs) beads using  Asplenium nidus  leaf extract and evaluate their antibacterial activity and colourimetric response toward Hg 2+  ions. AgNPs were biosynthesised using  Asplenium nidus  leaf extract and incorporated into a sodium alginate matrix to form SA/AgNPs nanocomposite beads. The formation of AgNPs was indicated by a colour change from clear yellow to brown and confirmed by UV–Vis absorption in the range of 420–450 nm. The resulting SA/AgNPs nanocomposite beads exhibited antibacterial activity against  Escherichia coli , producing an inhibition zone of approximately 1 mm, whereas sodium alginate beads without AgNPs showed no inhibition zone. In addition, the SA/AgNPs nanocomposite beads exhibited a concentration-dependent visual response toward Hg 2+  ions (10–80 ppm), with a distinct colour change from brown to white, while the beads exposed to demineralised water remained brown after 24 h. These findings demonstrate that  Asplenium nidus  leaf extract can facilitate the biosynthesis of AgNPs and that the resulting SA/AgNPs nanocomposite beads exhibit both antibacterial activity and a visible response toward Hg 2+  ions. The combination of a plant-mediated synthesis approach and alginate-based bead fabrication provides a simple platform with potential for antibacterial applications and preliminary colourimetric detection of Hg 2+  ions. },
   issn = {2597-9914},   pages = {559--567}  doi = {10.14710/jksa.29.8.559-567},
    url = {https://ejournal.undip.ac.id/index.php/ksa/article/view/85255}
}

Citation Format:

Abstract

Silver nanoparticles (AgNPs) have attracted considerable interest due to their antibacterial properties and potential for metal-ion sensing. This study aimed to develop sodium alginate/silver nanoparticle (SA/AgNPs) beads using Asplenium nidus leaf extract and evaluate their antibacterial activity and colourimetric response toward Hg²⁺ ions. AgNPs were biosynthesised using Asplenium nidus leaf extract and incorporated into a sodium alginate matrix to form SA/AgNPs nanocomposite beads. The formation of AgNPs was indicated by a colour change from clear yellow to brown and confirmed by UV–Vis absorption in the range of 420–450 nm. The resulting SA/AgNPs nanocomposite beads exhibited antibacterial activity against Escherichia coli, producing an inhibition zone of approximately 1 mm, whereas sodium alginate beads without AgNPs showed no inhibition zone. In addition, the SA/AgNPs nanocomposite beads exhibited a concentration-dependent visual response toward Hg²⁺ ions (10–80 ppm), with a distinct colour change from brown to white, while the beads exposed to demineralised water remained brown after 24 h. These findings demonstrate that Asplenium nidus leaf extract can facilitate the biosynthesis of AgNPs and that the resulting SA/AgNPs nanocomposite beads exhibit both antibacterial activity and a visible response toward Hg²⁺ ions. The combination of a plant-mediated synthesis approach and alginate-based bead fabrication provides a simple platform with potential for antibacterial applications and preliminary colourimetric detection of Hg²⁺ ions.

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Keywords: Asplenium nidus; Silver Nanoparticles; Nanocomposite; Antibacterial

Funding: Institute for Research and Community Service (LPPM), University of Bengkulu under contract 2941/UN.30.15/PT/2024

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Section: Research Articles

Language : EN

In Vol 29, No 8 (2026): Volume 29 Issue 8 Year 2026

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