KINETIC STUDY OF OIL PALM EMPTY FRUIT BUNCH ENZYMATIC HYDROLYSIS

Diah Meilany; Efri Mardawati; Made Tri Ari Penia Kresnowati; Tjandra Setiadi

doi:10.14710/reaktor.17.4.197-202

DOI: https://doi.org/10.14710/reaktor.17.4.197-202

KINETIC STUDY OF OIL PALM EMPTY FRUIT BUNCH ENZYMATIC HYDROLYSIS

Diah Meilany - Department of Chemical Engineering, Faculty of Industrial Engineering, Institute of Technology Bandung, Indonesia

Efri Mardawati - Department of Chemical Engineering, Faculty of Industrial Engineering, Institute of Technology Bandung, Indonesia

*Made Tri Ari Penia Kresnowati - Department of Chemical Engineering, Faculty of Industrial Engineering, Institute of Technology Bandung, Indonesia

Tjandra Setiadi - Department of Chemical Engineering, Faculty of Industrial Engineering, Institute of Technology Bandung

Received: 31 Jul 2017; Published: 2 Feb 2018.

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Abstract

As lignocellulosic biomass, Oil Palm Empty Fruit Bunch (OPEFB) can be used as the source of xylose that can be further utilized as the raw material for xylitol production. The processing of OPEFB to xylose comprises of pretreatment and hydrolysis that can be performed enzymatically. This process offers the advantages of moderate operation conditions and more environmentally friendly. This article describes the kinetic study of enzymatic hydrolysis process of OPEFB for producing xylose using self-prepared and commercial xylanase enzymes. Despite the possible mass transfer limitation, the Michaelis Menten kinetics was hypothesized. The results indicated that the reaction at pH 5 and 60°C followed the Michaelis Menten kinetics, with V_m of 0.84 g/L-h and K_m of 48.5 g/L for the commercial enzyme, and V_m of 0,38 g/L-h and K_m of 0,37 g/L for the self-prepared enzyme. The reaction is affected by temperature, with E_a of 8.6 kcal/gmol. The performance of self-prepared xylanase enzyme was not yet as good as the commercial enzyme, Cellic Htec 2.

Keywords: enzymatic hydrolysis; kinetics parameter; OPEFB; xylanase; xylose

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Funding: Indonesian Oil Palm Plantation Fund Management Agency

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Article Info

Section: Research Article

Language : EN

In Volume 17 No. 4 Desember 2017

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Last update: 2025-07-16 21:22:11

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