BibTex Citation Data :
@article{Reaktor32170, author = {Lucia Soedirga and Hardoko Hardoko and Natasha Widianto}, title = {N-Acetylglucosamine Production by Repeated-Batch Fermentation Using Immobilized Semi-Purified Chitinase Enzyme on Agar}, journal = {Reaktor}, volume = {21}, number = {1}, year = {2021}, keywords = {}, abstract = { Chitinolytic mold, such as Mucor circinelloidesis can be utilized to produce chitinase enzyme for shrimp shell’s chitin hydrolysis into N-acetylglucosamine (NAG). For that purpose, entrapment of chitinase on agar as a carrier could be an alternative way to improve NAG production. This study aimed to investigate the stability of immobilized semi-purified chitinase on agar for multiple cycles fermentation to produce NAG. In this study, 0.6 mL of semi-purified chitinase enzyme was immobilized into 3% of agar matrices and tested for four fermentation cycles to obtain highest NAG concentration and good enzyme activity. The results indicate that the immobilized chitinase could be used for 6 hours fermentation or three fermentation cycles. The NAG concentration produced after three cycle were 1042.22 ± 16.20 ppm. Besides, the immobilized enzyme was considerably stable up to the third cycles with activity value of about 4.74 U/mL. Keywords: agar; immobilized;NAG; repeated fermentation }, issn = {2407-5973}, pages = {11--14} doi = {10.14710/reaktor.21.1.11-14}, url = {https://ejournal.undip.ac.id/index.php/reaktor/article/view/32170} }
Refworks Citation Data :
Chitinolytic mold, such as Mucor circinelloidesis can be utilized to produce chitinase enzyme for shrimp shell’s chitin hydrolysis into N-acetylglucosamine (NAG). For that purpose, entrapment of chitinase on agar as a carrier could be an alternative way to improve NAG production. This study aimed to investigate the stability of immobilized semi-purified chitinase on agar for multiple cycles fermentation to produce NAG. In this study, 0.6 mL of semi-purified chitinase enzyme was immobilized into 3% of agar matrices and tested for four fermentation cycles to obtain highest NAG concentration and good enzyme activity. The results indicate that the immobilized chitinase could be used for 6 hours fermentation or three fermentation cycles. The NAG concentration produced after three cycle were 1042.22 ± 16.20 ppm. Besides, the immobilized enzyme was considerably stable up to the third cycles with activity value of about 4.74 U/mL.
Keywords: agar; immobilized;NAG; repeated fermentation
Article Metrics:
Last update:
Industrial Microbiology and Biotechnology
Last update: 2024-12-03 14:29:16
JURNAL REAKTOR (p-ISSN: 0852-0798; e-ISSN: 2407-5973)
Published by Departement of Chemical Engineering, Diponegoro University