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N-Acetylglucosamine Production by Repeated-Batch Fermentation Using Immobilized Semi-Purified Chitinase Enzyme on Agar

*Lucia Soedirga orcid scopus  -  Universitas Pelita Harapan, Indonesia
Hardoko Hardoko  -  Faculty of Fisheries and Marine Sciences, Universitas Brawijaya, Indonesia
Natasha V Widianto  -  Department of Food Technology, Faculty of Science and Technology,Universitas Pelita Harapan, Indonesia
Open Access Copyright (c) 2021 Reaktor under http://creativecommons.org/licenses/by-nc-sa/4.0.

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Abstract

Chitinolytic mold, such as Mucor circinelloidesis can be utilized to produce chitinase enzyme for shrimp shell’s chitin hydrolysis into N-acetylglucosamine (NAG). For that purpose, entrapment of chitinase on agar as a carrier could be an alternative way to improve NAG production. This study aimed to investigate the stability of immobilized semi-purified chitinase on agar for multiple cycles fermentation to produce NAG. In this study, 0.6 mL of semi-purified chitinase enzyme was immobilized into 3% of agar matrices and tested for four fermentation cycles to obtain highest NAG concentration and good enzyme activity. The results indicate that the immobilized chitinase could be used for 6 hours fermentation or three fermentation cycles. The NAG concentration produced after three cycle were 1042.22 ± 16.20 ppm. Besides, the immobilized enzyme was considerably stable up to the third cycles with activity value of about 4.74 U/mL.

Keywords: agar; immobilized;NAG; repeated fermentation

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Funding: thank CRCD (Centre for Research and Community Development) Universitas Pelita Harapan

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