BIOSINTESA SENYAWA FENOLIK ANTIOKSIDAN DARI LIMBAH KULIT PISANGKEPOK (Musa acuminata balbisiana C.) SECARA FERMENTASI SUBMERGED MENGGUNAKAN RHIZOPUS ORYZAE

Andre Siswaja; Adhitia Gunarto; Ery Susiany Retnoningtyas; Aning Ayucitra

doi:10.14710/reaktor.15.4.224-230

DOI: https://doi.org/10.14710/reaktor.15.4.224-230

BIOSINTESA SENYAWA FENOLIK ANTIOKSIDAN DARI LIMBAH KULIT PISANGKEPOK (Musa acuminata balbisiana C.) SECARA FERMENTASI SUBMERGED MENGGUNAKAN RHIZOPUS ORYZAE

Andre Siswaja - Universitas Katolik Widya Mandala Surabaya, Indonesia

Adhitia Gunarto - Universitas Katolik Widya Mandala Surabaya, Indonesia

*Ery Susiany Retnoningtyas - Universitas Katolik Widya Mandala Surabaya, Indonesia

Aning Ayucitra - Universitas Katolik Widya Mandala Surabaya, Indonesia

Received: 25 Jun 2015; Published: 7 Oct 2015.

BibTex Citation Data :

@article{Reaktor8697,
    author = {Andre Siswaja and Adhitia Gunarto and Ery Retnoningtyas and Aning Ayucitra},
    title = {BIOSINTESA SENYAWA FENOLIK ANTIOKSIDAN DARI LIMBAH KULIT PISANGKEPOK (Musa acuminata balbisiana C.) SECARA FERMENTASI SUBMERGED MENGGUNAKAN RHIZOPUS ORYZAE},
    journal = {Reaktor},
  volume = {15},
    number = {4},
    year = {2015},
    keywords = {antioksidan; biosintesa; fenolik; kulit pisang kepok; Rhizopus oryzae},
    abstract = {  BIOSYNTHESIS OF PHENOLIC ANTIOXIDANT COMPOUNDS FROM KEPOK BANANA PEEL WASTE ( Musa acuminata balbisiana C.)  USING SUBMERGED FERMENTATION  BY  RHIZOPUS ORYZAE .   Phenolic antioxidant compounds can be formed through a process of biosynthesis with the help of microorganism. Kepok banana peel waste contains nutrients that support the growth of Rhizopus oryzae producing phenolic antioxidant compounds through its secondary metabolism. The objective of this research was to study the effects of fermentation time, concentration of Kepok banana peel extracts, and concentration of (NH 4 ) 2 SO 4  on Total Phenolic Content (TPC) of extracts substrate. Total Antioxidant Capacity (TAC) of extracts with the highest TPC value was also measured. TPC of extracts were analyzed by Folin-Ciocalteu method whilst TAC by 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. Rhizopus oryzae was grown on a substrate containing kepok banana peel extract (500 g of kepok banana peel/L of water and 1000 g of kepok banana peel/L of water), (NH 4 ) 2 SO 4 , and other nutrients. Results showed that extracts with the highest phenolic content were obtained after 72 hours fermentation on substrate containing 32.69 mg/mL of glucose (concentration of kepok banana peel 1000 g/L of water) and 0.25% w/v (NH 4 ) 2 SO 4 . The substrate had TPC of 582.07 mg Gallic Acid Equivalent (GAE)/L extract with TAC of 88.37%.              Keywords:   biosynthesis; kepok banana peel; phenolic antioxidant; Rhizopus oryzae                    Abstrak           Senyawa fenolik antioksidan dapat terbentuk melalui proses biosintesa dengan bantuan Rhizopus oryzae menggunakan substrat limbah kulit pisang kepok. Limbah kulit pisang kepok belum banyak dimanfaatkan dan memiliki nilai ekonomi rendah. Di sisi lain, kulit pisang kepok mengandung sejumlah nutrisi yang dapat mendukung pertumbuhan Rhizopus oryzae dalam memproduksi senyawa fenolik antioksidan. Penelitian ini bertujuan untuk mempelajari pengaruh waktu fermentasi, konsentrasi ekstrak kulit pisang kepok, dan konsentrasi (NH 4 ) 2 SO 4  terhadap perolehan senyawa fenolik, serta mempelajari Total Antioxidant Capacity (TAC) untuk ekstrak dengan perolehan senyawa fenolik tertinggi. Dalam penelitian ini, Rhizopus oryzae ditumbuhkan pada substrat ekstrak kulit pisang kepok dengan variasi 500 g kulit pisang kepok/L air dan 1000 g kulit pisang kepok/L air serta penambahan (NH 4 ) 2 SO 4  dengan variasi konsentrasi. Total Phenolic Content (TPC) diukur menggunakan metode Folin-Ciocalteu, sedangkan Total Antioxidant Capacity (TAC) dianalisis dengan metode DPPH (2,2-diphenyl-1-picrylhydrazyl). Hasil penelitian menunjukkan bahwa TPC tertinggi terkandung dalam konsentrasi ekstrak kulit pisang kepok 1000 g/L air dengan  penambahan 0,25% b/v (NH 4 ) 2 SO 4 ) yaitu 582,07 mg Gallic Acid Equivalent (GAE)/L ekstrak dengan TAC 88,37% setelah fermentasi 72 jam.           Kata kunci:   biosintesa; kulit pisang kepok; fenolik  antioksidan; Rhizopus oryzae  },
   issn = {2407-5973},   pages = {224--230}  doi = {10.14710/reaktor.15.4.224-230},
    url = {https://ejournal.undip.ac.id/index.php/reaktor/article/view/8697}
}

Citation Format:

Abstract

BIOSYNTHESIS OF PHENOLIC ANTIOXIDANT COMPOUNDS FROM KEPOK BANANA PEEL WASTE (Musa acuminata balbisiana C.) USING SUBMERGED FERMENTATION BY RHIZOPUS ORYZAE. Phenolic antioxidant compounds can be formed through a process of biosynthesis with the help of microorganism. Kepok banana peel waste contains nutrients that support the growth of Rhizopus oryzae producing phenolic antioxidant compounds through its secondary metabolism. The objective of this research was to study the effects of fermentation time, concentration of Kepok banana peel extracts, and concentration of (NH₄)₂SO₄ on Total Phenolic Content (TPC) of extracts substrate. Total Antioxidant Capacity (TAC) of extracts with the highest TPC value was also measured. TPC of extracts were analyzed by Folin-Ciocalteu method whilst TAC by 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. Rhizopus oryzae was grown on a substrate containing kepok banana peel extract (500 g of kepok banana peel/L of water and 1000 g of kepok banana peel/L of water), (NH₄)₂SO₄, and other nutrients. Results showed that extracts with the highest phenolic content were obtained after 72 hours fermentation on substrate containing 32.69 mg/mL of glucose (concentration of kepok banana peel 1000 g/L of water) and 0.25% w/v (NH₄)₂SO₄. The substrate had TPC of 582.07 mg Gallic Acid Equivalent (GAE)/L extract with TAC of 88.37%.

Keywords: biosynthesis; kepok banana peel; phenolic antioxidant; Rhizopus oryzae

Abstrak

Senyawa fenolik antioksidan dapat terbentuk melalui proses biosintesa dengan bantuan Rhizopus oryzae menggunakan substrat limbah kulit pisang kepok. Limbah kulit pisang kepok belum banyak dimanfaatkan dan memiliki nilai ekonomi rendah. Di sisi lain, kulit pisang kepok mengandung sejumlah nutrisi yang dapat mendukung pertumbuhan Rhizopus oryzae dalam memproduksi senyawa fenolik antioksidan. Penelitian ini bertujuan untuk mempelajari pengaruh waktu fermentasi, konsentrasi ekstrak kulit pisang kepok, dan konsentrasi (NH₄)₂SO₄terhadap perolehan senyawa fenolik, serta mempelajari Total Antioxidant Capacity (TAC) untuk ekstrak dengan perolehan senyawa fenolik tertinggi. Dalam penelitian ini, Rhizopus oryzae ditumbuhkan pada substrat ekstrak kulit pisang kepok dengan variasi 500 g kulit pisang kepok/L air dan 1000 g kulit pisang kepok/L air serta penambahan (NH₄)₂SO₄dengan variasi konsentrasi. Total Phenolic Content (TPC) diukur menggunakan metode Folin-Ciocalteu, sedangkan Total Antioxidant Capacity (TAC) dianalisis dengan metode DPPH (2,2-diphenyl-1-picrylhydrazyl). Hasil penelitian menunjukkan bahwa TPC tertinggi terkandung dalam konsentrasi ekstrak kulit pisang kepok 1000 g/L air dengan penambahan 0,25% b/v (NH₄)₂SO₄) yaitu 582,07 mg Gallic Acid Equivalent (GAE)/L ekstrak dengan TAC 88,37% setelah fermentasi 72 jam.

Kata kunci: biosintesa; kulit pisang kepok; fenolik antioksidan; Rhizopus oryzae

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Keywords: antioksidan; biosintesa; fenolik; kulit pisang kepok; Rhizopus oryzae

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Section: Research Article

Language : ID

In Volume 15 No.4 Oktober 2015

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