CELL DISTRUPTION MIKROALGA SECARA ENZIMATIS DENGAN SELLULASE

Padil Padil; Siti Syamsiah; Muslikhin Hidayat; Rina Sri Kasiamdari

doi:10.14710/reaktor.15.4.213-217

DOI: https://doi.org/10.14710/reaktor.15.4.213-217

CELL DISTRUPTION MIKROALGA SECARA ENZIMATIS DENGAN SELLULASE

*Padil Padil - Jurusan Teknik Kimia, Fakultas Teknik Universitas Riau, Indonesia

Siti Syamsiah - Jurusan Teknik Kimia Universitas Gadjah Mada, Indonesia

Muslikhin Hidayat - Jurusan Teknik Kimia Universitas Gadjah Mada, Indonesia

Rina Sri Kasiamdari - Fakultas Biologi Universitas Gadjah Mada, Indonesia

Received: 27 Nov 2015; Published: 7 Jan 2016.

BibTex Citation Data :

@article{Reaktor9548,
    author = {Padil Padil and Siti Syamsiah and Muslikhin Hidayat and Rina Kasiamdari},
    title = {CELL DISTRUPTION MIKROALGA SECARA ENZIMATIS DENGAN SELLULASE},
    journal = {Reaktor},
  volume = {15},
    number = {4},
    year = {2016},
    keywords = {bioetanol, cell distruption, enzimatis, mikroalga, selulosa, pati},
    abstract = {  ENZYMATIC CELL DISRUPTION OF MICROALGAE USING CELLULASE.     Micro-algae is one source of potential alternative energy of third generation to be developed as bioethanol raw material. The starch content trapped in Micro-algae cell walls causing the need of cell distruption to release and convert starch into simple glucose before the fermentation process. This study aims to open up the cell walls of Micro-algae and to explore the effect of sellulase enzymes from Aspergillus niger as Micro-algae cell distruption strategy as well as the optimization of process parameters, i.e the concentration of enzyme, temperature, pH, and time which produce the highest glucose yield. The results showed that the highest glucose yield was 82.44% (w/w) obtained at an enzyme concentration of 30% (w/w), temperature 45 o C, pH of 4.5 at 40 minutes, the amount of Micro-algae as 0.5 g/L. In general, cell distruption method using sellulase enzyme was proven to be a promising option to open the cell walls of Micro-algae and convert cellulose into simple glucose simultaneously in producing bioethanol.            Keywords:   bioethanol; cellulose; cell distruption; enzymatic; micro-algae; starch                     Abstrak           Mikroalga merupakan salah satu sumber energi alternatif generasi ketiga yang potensial   untuk dikembangkan sebagai bahan baku bioetanol. Kandungan pati yang terperangkap dalam dinding sel mikroalga menyebabkan perlunya cell distruption untuk melepaskan sekaligus mengkonversi pati menjadi glukosa sederhana sebelum proses fermentasi. Penelitian ini bertujuan untuk membuka dinding sel mikroalga sekaligus mengeksplorasi pengaruh enzim sellulase dari aspergillus niger sebagai strategi cell distruption mikroalga serta melakukan optimasi parameter proses yaitu konsentrasi enzim, suhu, pH, dan waktu yang memberikan yield glukosa tertinggi. Hasil penelitian menunjukkan bahwa yield glukosa tertinggi adalah 82,44% (w/w) yang diperoleh pada konsentrasi enzim 30% (w/w), suhu 45 0 C, pH 4,5 pada waktu 40 menit, dengan jumlah mikroalga 0,5 g/L. Secara umum, metode cell distruption dengan menggunakan enzim sellulase terbukti menjadi pilihan yang menjanjikan untuk membuka dinding sel mikroalga sekaligus mengkonversi selulosa menjadi glukosa sederhana dalam memproduksi bioetanol.     },
   issn = {2407-5973},   pages = {213--217}  doi = {10.14710/reaktor.15.4.213-217},
    url = {https://ejournal.undip.ac.id/index.php/reaktor/article/view/9548}
}

Citation Format:

Abstract

ENZYMATIC CELL DISRUPTION OF MICROALGAE USING CELLULASE. Micro-algae is one source of potential alternative energy of third generation to be developed as bioethanol raw material. The starch content trapped in Micro-algae cell walls causing the need of cell distruption to release and convert starch into simple glucose before the fermentation process. This study aims to open up the cell walls of Micro-algae and to explore the effect of sellulase enzymes from Aspergillus niger as Micro-algae cell distruption strategy as well as the optimization of process parameters, i.e the concentration of enzyme, temperature, pH, and time which produce the highest glucose yield. The results showed that the highest glucose yield was 82.44% (w/w) obtained at an enzyme concentration of 30% (w/w), temperature 45^oC, pH of 4.5 at 40 minutes, the amount of Micro-algae as 0.5 g/L. In general, cell distruption method using sellulase enzyme was proven to be a promising option to open the cell walls of Micro-algae and convert cellulose into simple glucose simultaneously in producing bioethanol.

Keywords: bioethanol; cellulose; cell distruption; enzymatic; micro-algae; starch

Abstrak

Mikroalga merupakan salah satu sumber energi alternatif generasi ketiga yang potensial untuk dikembangkan sebagai bahan baku bioetanol. Kandungan pati yang terperangkap dalam dinding sel mikroalga menyebabkan perlunya cell distruption untuk melepaskan sekaligus mengkonversi pati menjadi glukosa sederhana sebelum proses fermentasi. Penelitian ini bertujuan untuk membuka dinding sel mikroalga sekaligus mengeksplorasi pengaruh enzim sellulase dari aspergillus niger sebagai strategi cell distruption mikroalga serta melakukan optimasi parameter proses yaitu konsentrasi enzim, suhu, pH, dan waktu yang memberikan yield glukosa tertinggi. Hasil penelitian menunjukkan bahwa yield glukosa tertinggi adalah 82,44% (w/w) yang diperoleh pada konsentrasi enzim 30% (w/w), suhu 45⁰C, pH 4,5 pada waktu 40 menit, dengan jumlah mikroalga 0,5 g/L. Secara umum, metode cell distruption dengan menggunakan enzim sellulase terbukti menjadi pilihan yang menjanjikan untuk membuka dinding sel mikroalga sekaligus mengkonversi selulosa menjadi glukosa sederhana dalam memproduksi bioetanol.

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Keywords: bioetanol, cell distruption, enzimatis, mikroalga, selulosa, pati

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Section: Research Article

Language : IND

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