DOI: https://doi.org/10.14710/ijfst.15.2.94-98

EFEK PEMBERIAN DELTAMETHRIN TERHADAP EKSPRESI CD4 PADA IKAN KOI (Cyprinus carpio) YANG TERINFEKSI Myxobolus sp. (Effect of Deltamethrin on CD4 Expression in Koi Fish (Cyprinus Carpio) Infected by Myxobolus Sp.)

*Muhammad Sumsanto  -  Brawijaya University, Indonesia
Uun Yanuhar  -  , Indonesia

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Abstract

Parasit yang sering menyerang ikan koi adalah Myxobolus sp. Ikan yang terinfeksi Myxobolus sp. akan mengalami gejala klinis berupa nodul kemerahan dan pembengkakan pada organ insang. Penelitian bertujuan untuk mengetahui pengaruh pemberian deltamethrin terhadap ekspresi CD4 pada ikan koi (Cyprinus carpio) yang terinfeksi Myxobolus sp. Pada penelitian ini dibagi menjadi 4 perlakuan (A) Kontrol, perlakuan (B) ikan koi terinfeksi Myxobolus sp., perlakuan (C) ikan koi terinfeksi Myxobolus sp. dengan pemberian deltamethrin dosis 0,5 µl/g dan perlakuan (D) ikan koi terinfeksi Myxobolus sp. dengan pemberian deltamethrin dosis 1 µl/g. Kemudian dilakukan pengamatan eritrosit, leukosit dan immunohistokimia untuk mengetahui ekspresi CD4 pada usus. Berdasarkan hasil penelitian didapatkan eritrosit pada perlakuan (A) sebesar 500.000 sel/mm3, (B) sebesar 1.850.000 sel/mm3, (C) sebesar 1.110.000 sel/mm3 dan (D) sebesar 1.670.000 sel/mm3. Hasil pengamatan leukosit perlakuan (A) didapatkan sebesar 121.600 sel/mm3, (B) sebesar 333.550 sel/mm3, (C) sebesar 265.770 sel/mm3 dan (D) sebesar 145.620 sel/mm3. Berdasarkan hasil Imunohistokimia  didapatkan hasil pada perlakuan (A) nilai DAB sebesar 15,0%, (B) DAB sebesar 30,2%, (C) DAB sebesar 24,3% dan (D) DAB sebesar 19,2 %. Dari penelitian ini dapat disimpulkan bahwa pemberian deltamethrin berpengaruh terhadap eritrosit dan leukosit serta dapat menurunkan ekspresi CD4 sebagai respon imun pada ikan koi (Cyprinus carpio) yang terinfeksi Myxobolus sp.

 

Parasites that often attack koi are Myxobolus sp. Fish infected with Myxobolus sp. will experience clinical symptoms of red nodules and swelling in the gill organs. The study aimed to determine the effect of deltamethrin administration on CD4 expression in koi fish (Cyprinus carpio) infected with Myxobolus sp. In this study divided into 4 treatments (A) Control, treatment (B) koi fish infected with Myxobolus sp., Treatment (C) koi fish infected with Myxobolus sp. by treatment a dose of 0.5 µl / g deltamethrin and treatment (D) koi fish infected with Myxobolus sp. with the treatment of 1 µl / g of deltamethrin. Then erythrocytes, leukocytes and immunohistochemistry were observed to determine CD4 expression in the intestine. Based on the results of the study obtained erythrocytes in treatment (A) of 500,000 cells / mm3, (B) of 1,850,000 cells / mm3, (C) of 1,110,000 cells / mm3 and (D) of 1,670,000 cells / mm3. Observation of leukocyte treatment (A) was obtained at 121,600 cells / mm3, (B) of 333,550 cells / mm3, (C) of 265,770 cells / mm3 and (D) of 145,620 cells / mm3. Based on the results of immunohistochemistry the results obtained in treatment (A) DAB value of 15.0%, (B) DAB of 30.2%, (C) DAB of 24.3% and (D) DAB of 19.2%. From this study it can be concluded that the administration of deltamethrin affects erythrocytes and leukocytes and can reduce CD4 expression as an immune response in koi fish (Cyprinus carpio) infected with Myxobolus sp.

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Keywords: Cyprinus carpio; Hematology; Erythrocytes, Leukocytes; Myxobolus sp.; Deltamethrin

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  1. Aly, S.M., Ahmed, Y.A.G., Ghareeb, A.A.A. and Mohamed, M.F., 2008. Studies on Bacillus subtilis and Lactobacillus acidophilus, as potential probiotics, on the immune response and resistance of Tilapia nilotica (Oreochromis niloticus) to challenge infections. Fish & shellfish immunology, 25(1-2), pp.128-136
  2. Ashfaq, H., Soliman, H., Saleh, M. and El-Matbouli, M., 2019. CD4: a vital player in the teleost fish immune system. Veterinary research, 50(1), p.1
  3. Asniatih, M. Idris dan K. Sabilu. 2013. Studi Histopatologi pada Ikan Lele Dumbo (Clarias garapinus) yang Terinfeksi Bakteri Aeromonas hydrophila. Jurnal Mina Laut Indonesia, FPIK Universitas Halu Oleo, Kendari, 3 (12): 13-21
  4. Azmi, H., D. R. Indriyanti, and N. Kariada, “Identifikasi Ektoparasit pada Ikan Koi (Cyprinus carpio L) di Pasar Ikan Hias Jurnatan Semarang,” Life Sci., vol. 2, no. 2, 2013
  5. Blaxhall, P.C. and Daisley, K.W., 1973. Routine haematological methods for use with fish blood. Journal of fish biology, 5(6), pp.771-781
  6. Chavda, D., S. Bhatt, R. A. Sreepada, and A. Sheth, “Pathogenicity of Myxobolus infection and its effect on protein expression in Catla catla in central Gujarat region,” J. Cell Tissue Res., vol. 10, no. 1, p. 2157, 2010
  7. Gusrina. 2008. Budidaya Ikan Jilid 2. Direktorat Pembinaan Sekolah Menengah Kejuruan Direktorat Jenderal Manajemen Pendidikan Dasar dan Menengah Departemen Pendidikan Nasional. Jakarta
  8. Hutapea, H. M. L., Y. Mirino, M. Widiyanti, E. Fitriana, Y. Maladan, and A. Oktavian, “PENURUNAN CD4 PADA ODHA SETELAH TERAPI ARV LEBIH DARI 39 BULAN,” Media Kesehat. Masy. Indones., vol. 13, no. 3, pp. 267–272, 2017
  9. Jalil, N., A. M. Adam, K. Djawad, A. Seweng, R. Halim, and A. Adriani, “Comparison of Total Antioxidant Capacity and Cd-4 in Patients with HIV Stage I and Stage IV,” Nusant. Med. Sci. J., vol. 2, no. 1, pp. 14–21, 2017
  10. Lukistyowati, I., 2011. Kelangsungan Hidup Ikan Mas (Cyprinus carpio L) yang Diberi Pakan Ekstrak Bawang Putih (Allium sativum) dan di Infeksi Aeromonas hydrophila. Jurnal perikanan dan kelautan, 16(02)
  11. Mahasri, G., Kismiyati. 2011. Buku Ajar Parasit dan Penyakit Ikan I (Ilmu penyakit Protozoa pada Ikan dan Udang). Universitas Airlangga.Surabaya. hal. 3-4
  12. Norsiah, Wahdah. 2015. Perbedaan kadar hemoglobin metode sianmethemoglobin dengan dan tanpa sentrifugasi pada sampel leukositosis. Medical Laboratory Technology Journal. I (2): 72-83 hal
  13. Nuryati, S., Soejoedono, R.D., Santika, A., Pasaribu, F.H. and Sumantadinata, K., 2010. Construction of a DNA vaccine using glycoprotein gene and its expression towards increasing survival rate of KHV-infected common carp (Cyprinus carpio). Jurnal Natur Indonesia, 13(01), pp.47-52
  14. Prihartini, N. C. dan A. Alfiyah, “MYXOSPOREASIS IN KOI (Cyprinus carpio),” Samakia J. Ilmu Perikan., vol. 8, no. 1, pp. 6–10, 2017
  15. Rahmaningsih, S., 2012. Pengaruh Ekstrak Sidawayah Dengan Konsentrasi Yang Berbeda Untuk Mengatasi Infeksi Bakteri Aeromonas hydrophilla Pada Ikan Nila (Oreochromis niloticus). AQUASAINS, 1(1), pp.1-8
  16. Randelli E, Buonocore F, Scapigliati G. 2008. Cell markers and determinants in fish immunology. Fish & Selfish Immunol. 25 : 326-340
  18. Sato A, Figueroa, Murray BW, Trillo EW, Rutczynska ZZ, Sultman H, Toyosawa S, Wedekind C, Steck N, Klein J. 2000. Nonlinkage of major histocompatibility complex class I and class II loci in bony fish. Immunogenetics. 51(2): 108-116
  19. Selvi, M., Çavaş, T., Cağlan Karasu Benli, A., Koçak Memmi, B., Çinkılıç, N., Dinçel, A.S., Vatan, Ö., Yılmaz, D., Sarıkaya, R., Zorlu, T. and Erkoç, F., 2013. Sublethal toxicity of esbiothrin relationship with total antioxidant status and in vivo genotoxicity assessment in fish (Cyprinus carpio L., 1758) using the micronucleus test and comet assay. Environmental toxicology, 28(11), pp.644-651
  20. Tizard IR. 2004. Veterinary Immunology : An Introduction. Ed ke-7. Amerika Serikat(USA) : Elsevier. Tort L, Balasch JC, Mackenzie S. 2003. Fish immune system. A crossroads between innate and adaptive responses. J Inmunologia. 22(3): 277-286
  21. Yusuf, M., Mahasri, G. and Mufasirin, M., 2019. Analisis Respons Imun Ikan Koi (Cyprinus carpio Koi) yang Divaksin dengan Whole Protein Spora Myxobolus koi sebagai Kandidat Vaksin Myxobolusis [Immune Response Analysis of Fish Koi (Cyprinus carpio Koi) Vaccinated Myxobolus Koi Spores Whole Protein as Vaccine Candidate Myxobolusis]. Jurnal Ilmiah Perikanan dan Kelautan, 7(1), pp.71-78

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