DOI: https://doi.org/10.14710/ijfst.17.1.%25p

PHYTOCHEMICAL SCREENING, TOTAL PHENOLIC CONTENT AND ANTIOXIDANT ACTIVITY OF TROPICAL BROWN MACROALGAE Padina pavonica HAUCK FROM KABUNG ISLAND, WEST KALIMANTAN

Phytochemical Screening, Total Phenolic Content and Antioxidant Activity of Tropical Brown Macroalgae Padina pavonica Hauck from Kabung Island, West Kalimantan

Mega Sari Juane Sofiana  -  Marine Science Department, Faculty of Mathematics and Natural Sciences, Tanjungpura University, Indonesia, Indonesia
Ikha Safitri  -  Marine Science Department, Faculty of Mathematics and Natural Sciences, Tanjungpura University, Indonesia, Indonesia
Shifa Helena  -  Marine Science Department, Faculty of Mathematics and Natural Sciences, Tanjungpura University, Indonesia, Indonesia
*Warsidah Warsidah  -  Marine Science Department, Faculty of Mathematics and Natural Sciences, Tanjungpura University, Indonesia, Indonesia

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Abstract
Marine macroalgae are potential sources as carbohydrate alternative, food additive, and bioactive compounds with antioxidant activity. The biological activity is strongly influenced by the type and phenolic compounds concentration. Tropical brown algae Padina pavonica Hauck was found with a large abundance in Kabung Island, West Kalimantan. The objective of this study was to determine the phytochemical screening, total phenolic content, and antioxidant activity of P. pavonica Hauck extract. The phytochemical screening and determination of total phenolic compounds were carried out using two different solvents with ethanol and ethyl acetate. Whereas antioxidant activity was evaluated using the DPPH (1,1-diphenyl-2-picrylhydrazyl) free radical scavenger method with UV-vis spectrophotometer. The qualitative analysis of phytochemical constituents revealed the presence of alkaloids, steroids, flavonoids, phenols, and saponins. Furthermore, for total phenolic compounds, the ethanolic extract had a higher content (20.34 mgGAE/g) dry sample than ethyl acetate extract (7 mgGAE/g). The ethanolic extract also had a higher of potential antioxidant activity which IC50 was 144.47 ppm compared to ethyl acetate extract (IC50 was 191.98 ppm).

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Phytochemical Screening, Total Phenolic Content and Antioxidant Activity of Tropical Brown Macroalgae Padina pavonica Hauck from Kabung Island, West Kalimantan
Subject phytochemical; phenol; antioxidant; Padina pavonic
Type Research Results
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Keywords: antioxidant; Padina pavonica; phenol; phytochemical

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Section: Research Articles
Language : EN
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  1. Abdolghaffari, A.H. (2010). On the benefit of Teucrium in murine colitis through improvement of toxic inflammatory mediators. Human Exp. Toxicol., 29, 287-295
  2. Al-Enazi, N.M., Awaad, A.S., Mohamed, E.Z., Alqasoumi, S.I. (2018). Antimicrobial, antioxidant and anticancer activities of Laurencia catarinensis, Laurencia majuscula and Padina pavonica extracts. Saudi Pharmaceutical Journal, 26(1), 44 – 52
  3. Bangol, E., Momuat, L.I., Abidjulu, J. (2014). Aktivitas antioksidan ekstrak etanol dan n-heksan dari daun rumput santa maria (Artemisia vulgaris L.) pada minyak ikan. Jurnal Ilmiah Sains, 14(2), 129-135
  4. Beppu, F., Hosokawa, M., Niwano, Y., Miyashita, K. (2012). Effects of dietary fucoxanthin on cholesterol metabolism in diabetic/obese KK-Ay mice. Lipids in Health and Disease, 11, 112
  5. Bernardini, G., Minetti, M., Polizzotto, G., Biazzo, M. (2018). Pro-apoptotic activity of French Polynesian Padina pavonica extract on human osteosarcoma cells. Marine Drugs, 16(12), 504
  6. Ismail, G.A., Gheda, S.F., Abo-Shady, A.M., Abdel-Karim,O.H. (2019). In vitro potential activity of some seaweeds as antioxidants and inhibitors of diabetic enzymes. Food Science and Technology
  7. Kuncoro, E.B. (2004). Akuarium Laut. Penerbit Kanisius.Yogyakarta
  8. Buyukokurodlu, M.E, Ggulcin, I., Oktay, M., Kufreviodlu, O.I. (2001). In vitro antioxidant properties of dantrolene sodium, Pharmacol Res., 44, 491-495
  9. Caf, F., ilma , Durucan, F., demir N (2015). Biochemical components of three marine macroalgae (Padina pavonica, Ulva lactuca and Taonia atomaria) from the levantine sea coast of Antalya, Turkey. J. Biodivers. Environ. Sci., 6, 401–411
  10. Chakraborty, K., Praveen, N.K., Vijayan, K.K., Syda, R.G. (2013). Evaluation of phenolic contents and antioxidant activities of brown seaweeds belonging to Turbinaria spp. (Phaeophyta, Sargassaceae) collected from Gulf of Mannar. Asian Pacific Journal of Tropical Biomedicine, 3(1), 6-8
  11. Chandini, S.K., Ganesan, P., Bhaskar, N. (2008). In vitro antioxidant activities of three selected brown seaweeds of India. Food Chemistry, 107, 707-713
  12. Eluvakkal, T., Sivakumar, S.R., Arunkumar. (2010). Fucoidan in some Indian brown seaweeds found along the coast gulf of manner. Int. J Bot., 6, 176-181
  13. Ganesan, P., Chandini, S.K., Bhaskar, N. (2008). Antioxidant properties of methanol extract and its solvent fractions obtained from selected Indian red seaweeds. Bioresource Technology, 99, 2717–2723
  14. Haryani, T.S., Triastinurmiatiningsih, Bina, L.S. (2014). Efektivitas Ekstrak Padina australis Sebagai Antibakteri Escerichia coli Penyebab Diare. Prosiding Seminar Nasional Biodiversitas V. Universitas Surabaya
  15. Holt, S. (2008). Seaweed for healing and weight loss. Nat. Benefit News, 2, 1-4
  16. Jang, H.D., Chang, K.S., Huang, Y.S., Hsu, C.L., Lee, S.H., Su, M,S. (2007). Principal phenolic phytochemicals and antioxidant activities of three Chinese medisinal plants. Food Chemistry, 103, 749–756
  17. Kadi, A. (2006). Beberapa Catatan Kehadiran Marga Sargassum di Perairan Indonesia. Jurnal Oseana, 4, 19-29
  18. Kumar, S. R., Hosokawa, M., & Miyashita, K. (2013). Fucoxanthin: A Marine Carotenoid Exerting Anti-Cancer Effects by Affecting Multiple Mechanisms. Mar. Drugs, 11, 5130-5147
  19. Kumaran, A., Karunakaran, R. (2006). Nitric oxide radixal scavenging active components from Phyllanthus emblica L. Plant Foods Hum. Nutr., 61, 1-5
  20. Kurniawati, I., Maftuch, Hariati, A.M. (2016). Penentuan pelarut dan lama ekstraksi terbaik pada teknik maserasi Gracilaria sp. serta pengaruhnya terhadap kadar air dan rendemen. Jurnal Ilmu Perikanan, 7(2), 72-77
  21. Limantara, L., Heriyanto. (2010). Studi komposisi pigmen kandungan fukosantin rumput laut cokelat dari perairan Madura dengan KCKT. J. Ilmu Kelautan, 15 (1), 23-32
  22. Maeda, H., Tsukui, T., Sashima, T., Hosokawa, M., Miyashita, K. (2008). Seaweed carotenoid, fucoxanthin, as a multi- functional nutrient. Asia Pac J. Clim. Nutr., 17, 196-199
  23. Masriani Fadly D Bohari B (2020) α-Glucosidase Inhibitory Activity of Ethanol Extract Obtained from Dillenia suffruticosa and Pycnarrhena cauliflora. Journal of Global Pharma Technology, 12, 881–887
  24. Matsukawa, R., Dubinsky, Z., Kishimoto, E., Masaki, K., Masuda, Y., Takeuchi, T., Chihara, M., Yamamoto, Y., Nikiand, E., Karube, I. (1997). A Comparison of screening methods for antioxidants activity in seaweeds, J. Appl. Phycol., 9, 29-35
  25. McDonald, S., Prenzler, P.D., Antolovich, M. and Robards, K. (2001). Phenolic content and antioxidant activity of olive extracts. Food Chemistry, 73, 73 – 84
  26. Melka, N.H. (2009). Uji Aktivitas Antioksidan Karaginan dalam Alga Merah Jenis Eucheuma spinosium dan Gracillaria verrucosa. Universitas Islam Negeri Malang. Malang
  27. Mise, T., Yasumoto, T. (2011). Simultaneous treatment of cancer cells lines with the anticancer drug cisplatin and the antioxidant fucoxanthin. Br. J.Pharmacol. Toxicol., 2(3), 127-131
  28. Ozsoy, N., Can, A., Yanardag, R., Akev, N. (2008). Antioxidant activity of Smilax excelsa L. leaf extracts. Food Chemistry, 110, 571–583
  29. Molyneux P. (2004). The use of stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin Journal Science Techology, 26(2), 211-219
  30. Peng, J., Yuan, J.P., Wu, C.F., Wang, J.H. (2011). Fucoxanthin, a marine carotenoid present in brown seaweeds and diatoms: metabolism and bioactivities relevant to human health. Mar. Drugs, 9, 1806-1828
  31. Pinteus, S., Silva, J., Alves, C., Horta, A., Fino, N., Rodrigues, A.I., Mendes, S., Pedrosa, R. (2017). Cytoprotective effect of seaweeds with high antioxidant activity from the Peniche coast (Portugal). Food Chem., 218, 591–599
  32. Rohman, A., Riyanto, S., Yuniarti, N., Saputra, W.R., Utami, R., Mulatsih, W. (2010). Antioxidant activity, total phenolic anda total flavonoid of extract and fraction of red fruit (Pandanus conoideus Lam). Int. Food Res J., 97-106
  33. Fernando, I.P.S., Sanjeewa, K.K.A., Samarakoon, K. W., Lee, W. W., Kim, H. S., Ranasinghe, P., Gunasekara, U.K.D. S.S., Jeon, Y.J. (2018). Antioxidant and anti- inflammatory functionality of ten Sri Lankan seaweed extracts obtained by carbohydrase assisted extraction. Food Science and Biotechnology, 27(6), 1761-1769
  34. Sivanandham, V. (2011). Free radicals in health and diseases - a mini review. Pharmacologyonline, 1, 1062-1077
  35. Smith, A.J. (2004). Medicinal and pharmaceutical uses of seaweed natural products. Journal of Applied Phycology, 16, 245-262
  36. Vimala, M., Reginald, M., Irene, W.J. (2015). Phytochemical analysis in different solvent extracts of Padina tetrastromatica. International Journal of Development Research, 5(03), 3761-3763
  37. Wijesekara, I., Kim, S.K. (2010). Angiotensin-I-Converting Enzyme (ACE) inhibitors from marine resources. Mar Drugs, 8, 1080-1093
  38. Wijesekara, I., Yoon, N.Y., Kim, S.K. (2010). Phlorotannins from Ecklonia cava (Phaeophyceae): Biological activities and potential health benefits. Biofactors, 36, 408-414
  39. Wijesekara, I., Pangestuti, R., Kim, S.K. Biological activities and potential health benefits of sulfated polysaccharides derived from marine algae. Carb Polymer, 84, 14-21
  40. Young, I. S., Woodside, J. V. (2001). Antioxidants in health and disease. J. Clin. Pathol., 54, 176–86
  41. Yuan, Y.V., Carrington, M.F., Walsh, N.A. (2005). Extracts from dulse (Palmaria palmata) are effective antioxidants and inhibitors of cell proliferation in vitro. Food and Chem. Toxicol., 43, 1073-1082

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