skip to main content

SUCCESSFUL GENETIC CHARACTERIZATION OF BULLET TUNA (Auxis rochei) USING MICROSATELLITE MARKERS IN KARANGASEM BALI

*Maya Agustina scopus  -  Loka Riset Perikanan Tuna, Indonesia
Made Pharmawati  -  Universitas Udayana, Indonesia
Ketut Junitha  -  Universitas Udayana, Indonesia

Citation Format:
Abstract

Bullet Tuna (Auxis rochei) is classified under the neritic tuna group, which plays an essential role in small-scale fisheries in Karangasem Bali. The increasing catch of bullet tuna indicated its stock probably under threat. Therefore, genetic characterization is often required as the first step before building a conservation program. This study aims to categorize bullet tuna DNA using microsatellite. Of all five loci used, all were high polymorphism-type, with the number of alleles per locus varied between 18-27. Successful PCR (Polymerase Chain Reaction) created relatively high DNA concentration, ranging from 27.050 to 237.05 ng/ul, with a DNA purity level ranging from 2.073 to 2.239. Overall, the stock condition allegedly still in good condition, marked with high genes diversity (Ho=0.367-0.767 and He=0.934-0.966). All loci used can be amplified and well described.

Fulltext View|Download
Keywords: Neritic tuna; microsatellite; DNA; genetic diversity

Article Metrics:

  1. Agus, S. (2017). Mapping of Tuna (Euthynnus sp) Fishing Areas in Bone Bay Waters (Skripsi). Skripsi Hasanuddin University, Makasar. 56 hal
  2. Ariyanti, Y. & Sianturi, S. (2019). Extraction of Total DNA from Animal Tissue Sources (Grouper) using Method Kit for Animal Tissue. Journal of Science and Applicative Technology, 3(1): 40-45. https://doi.org/10.35472/jsat.v3i1.111
  3. Catanese, G., Infante, C., Crespo, A., Zuasti, E., Ponce, M., Funes, V., Perez, L. & Manchado, M. (2007). Development and characterization of eight microsatellite markers in bullet tuna (Auxis rochei). Molecular Ecology Notes, 7: 842 -844. DOI: 10.1111/j.1471-8286.2007.01723.x
  4. Collette, B.B., & Nauen, C.E. (1983). Scombrids of the world: An Annotated and Illustrated Catalogue of Tunas, Mackerels, Bonitos and Related Species Known to Date. FAO Fisheries Synopsis, 2(125): 1-129
  5. Excoffier, L., Laval, G. & Schneider, S. (2005). ARLEQUIN (Version 3.0): An Integrated Software Package for Population Genetics Data Analysis. Evolutionary Bioinformatics Online, 1: 47-50. http://dx.doi.org/10.1177/117693430500100003
  6. Ferguson, A.J.B., Taggart, P.A., Prodohl, O., Mc Meel, C., Thompson, C., Mc. Stone, Ginnity, R.A. & Hynes. (1995). The Application of molecular markers to the study & conservation of fish population with special referens to Salmon. Journal of Fish Biology, 47: 103-126. http://dx.doi.org/10.1111/j.1095-8649.1995.tb06048.x
  7. Hajibabaei M, Smith MA, Janzen DH, Rodriguez JJ, Whitfield JB, Hebert PDN. (2006). A minimalist barcode can identify a specimen whose DNA is degraded. Molecular Ecology Notes. 6(4): 959-964. https://doi.org/10.1111/j.1471-8286.2006.01470.x
  8. Herrera, M & L. Pierre. (2009). Status of IOTC databases for neritic tunas. IOTC-2009-WPDCS-06, 1-46
  9. Ilhamdi, H., Telussa, R., & Ernaningsih, D. (2016). Analysis of utilization rate and pelagic fishing season in Prigi Waters, East Java. Jurnal Ilmiah Satya Mina Bahari, 1(1), 52-64
  10. Indian Ocean Tuna Commission (IOTC). (2019). Report for the 23rd session of the Indian Ocean Tuna Commission. IOTC–2019–S23–R[E]: 1-105
  11. Lestami, A. (2019). Diversity and genetic identification of soybean mutant (Glycine max (L) Merril) resistant Athelia rolfsii Curzi based on SSR markers. Thesis Sumatera Utara University. Medan. 80 hal
  12. Mulyani Y., Purwanto A. & Nurruhwati I. (2011). Comparison of Several DNA Isolation Methods for Early Detection of Koi Herpes Virus (KHV) in Carp (Cyprinus carpio L.). Jurnal Akuatika. Vol 2, (1) : 1-16
  13. Naderi, R.A. (2016). Assessment of Social Consideration on Neritic Tuna in Iran Fishery Management. IOTC-2016-WPNT06-12, Rev_1: 1-14
  14. Nei, M. (1978). Estimation of average heterozygosity and genetic distance from a small number of individuals. Center for Demographic and Population Genetics, University of Texas at Houston, Texas, 89: 583 – 590
  15. Park, L. K., & Moran, P. (1994). “Developments in molecular genetic techniques in fisheries”. Reviews in Fish Biology and Fisheries, 4(3), 272–299. DOI: 10.1007/BF00042906
  16. Prayoga, I.M.S., Putra, I.D.N.N. & Dirgayusa, I.G.N.P. (2017). Effect of Distribution of Chlorophyll-a Concentration Based on Satellite Imagery on Tuna Catches (Euthynnus sp) in Bali Waters. Journal of Marine and Aquatic Sciences, 3(1): 30-46. https://doi.org/10.24843/jmas.2017.v3.i01.30-46
  17. Puja, I.K. & Sulabda, I.N. (2009). Genetics Characteristic of Gembrong Goat from Karangasem Bali Using Microsatellite DNA. Biota Vol. 14 (1): 45-49. https://doi.org/10.24002/biota.v14i1.2632
  18. Sambrook, J. dan D. W. Russell. (2001). Molecular Cloning: A Laboratory Manual. 3 rd Edition. Cold Spring Harbor Laboratory Press, New York. 2000p
  19. Santos, S., Hrbek, T., Farias, I.P., Schneider, H., & Sampaio, I. (2006). Population genetic structuring of the king weakûsh, Macrodon ancylodon (Sciaenidae), in Atlantic coastal waters of South America: deep genetic divergence without morphological change. Molecular Ecology, 15:4361–4373. https://doi.org/10.1111/j.1365-294X.2006.03108.x
  20. Sastra I.G.A.B.W., Karang, I.W.G.A., As-syakur, A.R. & Suteja, Y. (2018). Seasonal Variation of the Relationship between Oceanographic Parameters and Tuna Catches based on Daily Data in the Bali Strait. Journal of Marine and Aquatic Sciences, 4(1): 109-119. https://doi.org/10.24843/jmas.2018.v4.i01.109-119
  21. Sentani R.S.A., Hafy Z. & Subandrate. (2017). Relationship of deparaffinization method with quantity and quality of isolated DNA extracts from tissue archive samples in formalin-fixed paraffin blocks. Jurnal Kedokteran dan Kesehatan, 4(1). 32-38
  22. Ulfa, M. & Retnoningsih, A. (2010). Microsatellite Markers as Teak Plus Characteristics. Floribunda 4(1). 5-14. https://doi.org/10.32556/floribunda.v4i1.2010.80
  23. Ulupi, N., Muladno, Sumantri, C. & Wibawan, I.W.T. (2014). The Genetic Polimorphism Of Toll-Like Receptor-4 Gene in Local Chickens Using Polymerase Chain Reaction-Restriction Fragment Lenght Polymorphism. Jurnal Veteriner. Vol 15(3): 345-352. http://repository.ipb.ac.id/handle/123456789/76704
  24. Wandia, IN, Putra IGAA,, Soma IG. (2009). Genetic Polymorphism of Long-tailed Monkey Population at a tourism site, Bali. Faculty of Veterinary Medicine. Fundamental Report of Udayana University DIPA Funds for the 2009
  25. Zedta, R.R. & Setyadji, B. (2019). PCR Optimization of Frigate and Bullet Tuna on Genetic Diversity Analysis. Bawal, 11(2): 95-102. http://dx.doi.org/10.15578/bawal.11.2.2019.95-102

Last update:

No citation recorded.

Last update: 2024-03-27 17:43:23

No citation recorded.