DOI: https://doi.org/10.14710/ijfst.20.2.%25p

Effect of Sperm Extender and Dilution Ratio on The Sperm Motility, Fertility and Hatchability of Depik fish Rasbora Tawarensis (Pisces: Cyprinidae) Eggs

*Zainal Abidin Muchlisin orcid scopus  -  Faculty of Marine and Fisheries, Universitas Syiah Kuala, Indonesia
Dhea Febby Aldila  -  Universitas Syiah Kuala, Indonesia
Putri Inten Sarah  -  Universitas Syiah Kuala, Indonesia
Kartini Eriani  -  Universitas Syiah Kuala, Indonesia
Iwan Hasri  -  Dinas Perikanan Kabupaten Aceh Tengah, Indonesia
Nur Fadli  -  Universitas Syiah Kuala, Indonesia
Abdullah Abbas Muhammadar  -  Universitas Syiah Kuala, Indonesia
Mohd Nor Siti-Azizah  -  Universiti Malaysia Terengganu, Malaysia
Filiz Kutluyer Kocabas  -  Munzur University, Turkey

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Abstract

The artificial breeding method for depik fish Rasbora tawarensis was not available, and the extenders and its dilution ratio are the crucial factors in artificial breeding of fishes. Herein, we tested several common sperm extenders and its dilution ratio  in the artificial breeding for depik fish. Two  series of experiments were performed to find the best extender and its dilution ratio. In the first experiment, five types of extenders, namely: Ringer’s solution, physiological solution, coconut water, sugarcane water, and roomie water. The second experiment studied four dilution levels of sperm and extender (v/v), namely: 1: 20, 1: 30, 1: 40, and 1: 50. Every treatment was conducted in four replicates and experiments were conducted in series so that the best results in the first experiment were used in the second.  The first experiment showed that Ringer’s is the best extender for depik sperm, and the second experiment revealed that Ringer’s at dilution ratio 1: 40 gave higher sperm motility, fertilization and hatching rates. Hence,  the best formula for the dilution solution of depik sperm is Ringer’s at the dilution ratio of 1: 40, sperm: extender (v/v).

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Keywords: Ringer’s solution; Physiological solution; Threatened species; Endemic fish; Fish breeding

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Section: Research Articles
Language : EN
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  1. Adami, Y., N. Fadli, N. Nurfadillah, K. Eriani, Z. Jalil and Z.A. Muchlisin. (2016). A Preliminary Observation on The Effect of Sperm Extenders on The Fertilization and Hatching Rates of Seurukan Fish (Osteochilus vittatus) Eggs. AACL Bioflux, 9, 300-304
  2. Adipu, Y., H. Sinjal and J. Watung. (2011). The Effect of Extender Ratio on The Sperms Motility, Fertilization and Hatching Rate of Catfish, Clarias sp. Jurnal Perikanan dan Kelautan Tropis, 7, 48-55
  3. Agarwal, N.K. (2011). Cryopreservation of Fish Semen. Himalayan Aquatic Biodiversity Conservation & New Tools in Biotechnology Editors: Madhu Thapliyal, Ashish Thapliyal, JP Bhatt Transmedia Publication. Srinagar, India
  4. Alavi, S. M. H. and J. Cosson. (2006). Sperm Motility in Fishes. (II) Effects Of Ions And Osmolality: A Review. Cell Biology International, 30, 1-14
  5. Ahn, J. Y., J. Y. Park and H. K. Lim. (2018). Effects Of Different Diluents, Cryoprotective Agents, and Freezing Rates on Sperm Cryopreservation in Epinephelus akaara. Cryobiology, 83, 60-64
  6. Barlina, R., S. Karouw, J. Towaha and R. Hutapea. (2007). Effect of Coconut Water and Coconut Water Ratio and Storage Time on Quality of The Coconut Drink Powder. Jurnal Littri, 13, 73–80
  7. Butts, I. A. E., S. M. H. Alavi, A. Mokdad and T. E. Pitcher. (2013). Physiological Functions of Osmolality and Calcium Ions on The Initiation of Sperm Motility and Swimming Performance in Redside Dace Clinostomus elongatus. Comparative Biochemistry and Physiology Part A., 166, 147–157
  8. Chapman, F.A. (2016). A Semen Extender for The Short-Term Storage of Fish Sperm. Program in Fisheries and Aquatic Sciences, School of Forest Resources and Conservation. UF/IFAS Extension, University of Florida, USA
  9. Eriani, K., M. Mustaqim, I. Hasri, R. Amalia, A. Azhar, F. M. Nur and Z. A. Muchlisin. (2022). Effect of Fetal Bovine Serum on the Sperm Quality of Depik (Rasbora tawarensis) after Short-term Cryopreservation. Indian Journal of Animal Research, 56(7), 843-847
  10. Gao, Y., S. K. Kim and J. Y. Lee. (2011). Effects of pH on Fertilization and The Hatching Rates of Far Eastern Catfish (Silurus asotus). Fisheries and Aquatic Sciences, 41, 417-420
  11. Lim, H. K. and M. H. Le. (2013). Evaluation Of Extenders And Cryoprotectants On Motility And Morphology Of Longtooth Grouper (Epinephelus bruneus) Sperm. Theriogenology, 79(5), 867-871
  12. Maulida, S., K. Eriani, N. Fadli, F. M. Nur, L.S. Handayani and Z. A. Muchlisin. (2022). The Effect of Extender on The Quality of Climbing Perch (Anabas Testudineus) Sperm After Refrigerated Storage. Aquaculture Research, 53, 4497-504
  13. Muchlisin, Z. A., R. Hashim and A.S. Chong. (2004). Preliminary Study on The Cryopreservation of Bagrid Catfish Spermatozoa: Effect of Different Extenders and Cryoprotectants on The Motility After Short-Term Storage. Theriogenology, 62, 25-37
  14. Muchlisin, Z. A., M. Musman and M. N. Siti-Azizah. (2010a). Spawning Seasons of Rasbora tawarensis (Pisces: Cyprinidae) in Lake Laut Tawar, Aceh Province, Indonesia. Reproductive Biology and Endocrinology, 8, 49
  15. Muchlisin, Z. A., N. Nadiya, W. N. Nadiah, M. Musman and M. N. Siti-Azizah. (2010b). Preliminary Study on The Natural Extenders for Artificial Breeding of African Catfish Clarias gariepinus (Burchell, 1822). AACL Bioflux, 3, 119-124
  16. Muchlisin, Z. A., N. Fadli, E. Rudi, T. Mendo and M.N. Siti-Azizah. (2011). Estimation Of Production Trend of The Depik, Rasbora tawarensis (Teleostei, Cyprinidae), in Lake Laut Tawar, Indonesia. AACL Bioflux, 4, 590-597
  17. Muchlisin, Z. A., Z. Thomy, N. Fadli, M. A. Sarong and M. N. Siti-Azizah. (2013). DNA Barcoding of Freshwater Fishes from Lake Laut Tawar, Aceh Province, Indonesia. Acta Ichthyologica et Piscatoria, 43(1), 21-29
  18. Muchlisin, Z. A. (2013). Distributions of The Endemic and Threatened Freshwater Fish Depik, Rasbora tawarensis Weber & de Beaufort, 1916 in Lake Laut Tawar, Aceh Province, Indonesia. Songklanakarin Journal of Science and Technology, 35(4), 483-488
  19. Muchlisin, Z. A., W.N. Nadiah, N., Nadiya, N. Fadli, A. Hendri, M. Khalil and M. N. Siti-Azizah. (2015). Exploration of Natural Cryoprotectants For Cryopreservation Of African Catfish, Clarias gariepinus, Burchell 1822 (Pisces: Clariidae) Spermatozoa. Czech Journal of Animal Science, 60, 10–15
  20. Muchlisin, Z. A., I. Hasri and A. S. Batubara. (2018). A mini Review on Endemic and Threatened Fish Rasbora tawarensis in Lake Laut Tawar, Indonesia. IOP Conference Series: Earth and Environmental Sciences, 2016, 012045
  21. Muchlisin, Z. A., P.I. Sarah, D. F. Aldila, K. Eriani, I. Hasri, A. S. Batubara, F. M. Nur, M. Mustaqim, C. R. Muthmainnah and A. Abinawanto. (2020). Effect of Dimethyl Sulfoxide (DMSO) and Egg Yolk on Sperm Motility, Fertility and Hatching Rates of Depik Rasbora tawarensis (Pisces: Cyprinidae) Eggs After Short‐Term Cryopreservation. Aquaculture Research, 51(4), 1700-1705
  22. Muchlisin, Z. A., D. Afriani, K. Eriani, I. Hasri, F.M. Nur, S. Maulida, L.S. Handayani, N. Fadli, F.K. Kocabas, M.N. Siti-Azizah and M. Wilkes. 2023. Improvement of Sperm Quality of The Depik Fish, Rasbora tawarensis, After Cryopreservation Using Antioxidant. Cryo Letter, 44(1), 13-19
  23. Muthmainnah, C. R., K. Eriani, I. Hasri, M. Irham, A.S. Batubara and Z.A. Muchlisin. (2018). Effect of Glutathione on Sperm Quality After Short-Term Cryopreservation in Seurukan Fish Osteochilus vittatus (Cyprinidae). Theriogenology, 122, 30-34
  24. Suquet, M., C. Dreanno, C.,Fauvel, J. Cosson and R. Billard. (2000). Cryopreservation of Sperm in Marine Fish. Aquatic Research, 31, 231-243
  25. Williot, P., E. F. Kopeika and B. F. Goncharov. (2000). Influence of Testis State, Temperature and Delay in Semen Collection Spermatozoa Motility in The Cultured Siberian Sturgeon (Acipenser baeri, Brandt). Aquaculture, 189, 53-61
  26. Wilson-Leedy, J. G., M. K. Kanuga and R. L. Ingermann. (2009). Influence of Osmolality And Ions on The Activation and Characteristics of Zebrafish Sperm Motility. Theriogenology, 71, 1054-1062
  27. Zhou, J., L. Chen, J. Li, H. Li, Z. Hong, M. Xie, S. Chen and B. Yao. (2015). The Semen pH Affects Sperm Motility and Capacitation. PloS One, 10, e0132974

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