DOI: https://doi.org/10.14710/teknik.v38i2.10306

Optimization of Penicillium Lagena Medium Cultivication on Antifungal Pathogen of Phellinus Lamaoensis Using Surface Methode

*Siti Nabilah  -  Departement Bioteknologi, Institut Pertanian Bogor, Indonesia
Rofiq Sunaryanto  -  Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi, Indonesia
Khaswar Syamsu  -  Departemen Teknologi Agroindustri, Institut Pertanian Bogor, Indonesia
Open Access Copyright (c) 2017 Teknik

Citation Format:
Abstract

Phellinus lamaoensis (Murr.) Hein is fungal pathogen that can cause brown root rot disease in cocoa, tea, rubber, and coffee plants. Endophytic fungi, Penicillium lagena, isolated from bandotan (Ageratum conyzoides Linn.), medicinal plant, is able to inhibit the growth of pathogenic, P. lamaoensis. The effect of carbon source, nitrogen source, and mineral solution was studied. Lactose, yeast extract, and mineral solution were media components which showed significant effect toward production of P. lagena active compound. Composition optimization of these three medium components was done by response surface methodology (RSM). The Optimal response region of the significant factor was predicted by using a second order polynomial model with statistical design, central composite design (CCD). Higest production of P. lagena active compound by quadratic model was predicted to be 69.233%  with medium composition 44.77 g L-1 lactose, 13.02 g L-1 yeast extract, and 15.95 mL L-1 mineral solution. Verification value in laboratory is 58.365%, lower 15.7% than its prediction. Optimization increase P. lagena active compound 9 fold compared to unoptimize media.

Fulltext View|Download
Keywords: active compound; optimization; Penicillium lagena; RSM
Funding: Center of Biotechnology, Agency of the Assessment and Application of Technology (BPPT)

Article Metrics:

Article Info
Section: Artikel
Language : ID
Recent articles
Penurunan Kadar Kromium Heksavalen (Cr6+) Dalam Limbah Batik Menggunakan Limbah Udang (Kitosan) Pembuatan Briket Arang Dari Campuran Tempurung Kelapa dan Serbuk Gergaji Kayu Sengon Aplikasi Penginderaan Jauh dan EPA-SWMM untuk Simulasi Debit Banjir Akibat Perubahan Lahan Sub DAS Banjaran Back-matter Front-matter More recent articles
Most cited articles
ANALISIS RESIKO KONSENTRASI DEBU (TSP) DAN TIMBAL (Pb) DI PINGGIR JALAN TERHADAP KESEHATAN MANUSIA STUDI KASUS KOTA YOGYAKARTA Pengaruh Activity Support terhadap Kualitas Visual Koridor Jalan K.H. Agus Salim Semarang KELEMBAGAAN IMPLEMENTASI PROGRAM KREDIT MIKRO PERUMAHAN DI KOTA SEMARANG LIFE CYCLE ASSESSMENT OF PAPER TOWEL AND ELECTRIC DRYER AS HAND DRYING METHOD IN THE UNIVERSITY OF MELBOURNE UNJUK KERJA REAKTOR PLASMA DIELECTRIC BARRIER DISCHARGE UNTUK PRODUKSI BIODIESEL DARI MINYAK KELAPA SAWIT More cited articles
  1. Bradley, N. (2007). The respone surface methodology. Thesis. Indiana University of South Bend
  2. Crueger, W., Crueger, A. (1984). Biotechnology. USA: Science Tech, Inc
  3. Dalimartha, S. (2006). Atlas Tumbuhan Obat Indonesia Jilid 2. Jakarta: Trubus Agriwidya
  4. Eden, T. (1965). Tea. London: Longmans, Green and Co ltd
  5. Edwards, V.H. (1970). The Influence of High Substrat Concentration on Microbial Kinetics. Biotechnology and Bioengineering, 12, 679-712
  6. Ghatora, S.K., Chandha, B.S., Badhan, A.K, Saini, H.S., , Bhat, M.K. (2006). Identification and Characterization of Diverse Xylanases from Thermophilic and Thermotolerant Fungi. Bio Resources, 1(1), 18-33
  7. Kaswati, N.M.N. (2013). Penapisan dan Identifikasi Senyawa Antijamur Patogen Phellinus lamaoensis dari Jamur Endofit pada Tanaman Obat Asal Cirebon. Skripsi. Institut Pertanian Bogor
  8. Lepsch, R.A, McMillin, M,L. (1998). Response Surface Model Building and Multidisciplinary Optimization Using D-Optimal Design. American Institute of Aeronautics and Astronautics, 98, 47-59
  9. Liu, B.L & Tzeng, Y.M. (1998). Optimazation of Growth Medium for Production of Spores from Bacillus thuringiensi Using Reponse Surface Methodology. Bioprocess and Biosystems Engineering, 18, 413-418
  10. Lough, W.J., Wainer, I.W. (1996). High Performance Liquid Chromatography. Glasgow: Blackie Academic and Professional
  11. Mejía, L.C., Rojas, E.I., Maynard, Z., Bael, S.V., Arnold, A.E., Hebbar, P., Samuel, G.J., Robbins, N., & Herre, E.A. (2008). Endophytic Fungi as Biocontrol Agents of Theobroma cacao Pathogens. Biological Control, 46, 4-14
  12. Montgomery, D.C. (2001). Design and Analysis of Experiments 5th Edition. USA: John Wiley & Sons, Inc
  13. Nazaruddin, Paimin, F.B. (1992). Karet. Bogor: Penebar Swadaya
  14. Pusat Penelitian Kopi dan Kakao Indonesia. (2010). Buku Pintar Budi Daya Kakao. Jakarta: Agro Media Pustaka
  15. Selim, K.A., El-Beih, A.A., Abd El-Rahman, T.M., El-Diwany, A.I. (2012). Biology of Endophytic Fungi. Current Research in Environmental and Applied Mycology, 2(1), 31-82.doi: 10.5943/cream/2/1/3
  16. Semangun, H. (2000). Penyakit-Penyakit Tanaman Perkebunan di Indonesia. Yogyakarta: Gadjah Mada University Press
  17. Stanbury, P.F., Whitaker, A, Hall, S.J. (2003). Principles of Fermentation Technology 2nd Edition. Great Britain: MPG Books Ltd
  18. Tran, H.B.Q., McRae, J.M., Lynch, F., Palombo, E.A. (2010). Identification and Bioactive Properties of Endophytic Fungi Isolated from Phyllodes of Accacia Species. Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology, 1, 377-382
  19. Triharso. (1994). Dasar-Dasar Perlindungan Tanaman. Yogyakarta: Gadjah Mada University Press
  20. Wilson, E.O. (1988). Biodiversity. Washington DC: National Academy Press
  21. Wood, G.A.R., Lass, R.A. (1989). Cocoa. New York: John Wiley & Sons, Inc

Last update:

No citation recorded.

Last update: 2024-12-25 22:59:40

No citation recorded.