Kinetika detoksifikasi umbi gadung (dioscorea hispida dennst.) secara fermentasi dengan kapang mucor racemosus

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Article Info
Submitted: 17-05-2017
Published: 07-06-2017
Section: Research Article



THE KINETICS OF GADUNG (Dioscorea hispida dennst.) TUBER DETOXIFICATION VIA FUNGAL FERMENTATION USING Mucor racemosus. Bitter yam (Dioscorea hispida Dennst.) is one of carbohydrate sources used as staple food commonly found in Indonesian dry-land. However, this tuber has been underutilized due to the presence of an antinutrition compound, namely cyanogenic glucosides. Removal of cyanides from foodstuffs can be done either by physical, chemical or biological methods. In this study, the effect of time on the detoxification of gadung tuber chips from cyanides via fermentation using Mucor racemosus and its kinetics were investigated. Gadung chip samples were withdrawn from the fermentation system at every 24 hours interval for biomass and cyanides contents analysis. It was clear that the cyanides content decreased as the fermentation went by. Safely consumed gadung tuber chips were obtained from fermentation of the chips for 120 hours from which cyanides content as low as 49.41 mg/kg was achieved. The logistic equation successfully described the growth rate of Mucor racemosus under studied condition. The specific growth rate of Mucor racemosus in gadung chips was found to be 0.0297/hr or about a half of specific growth rate of that mold when grown in the readily consumed yeast-pepton-glucose (YPG) media.


Keywords: fermentation; yam; monod; Mucor racemosus; cyanogen





Umbi gadung (Dioscorea hispida Dennst.) merupakan salah satu sumber karbohidrat yang digunakan sebagai makanan pokok yang biasa ditemukan di lahan kering di wilayah Indonesia. Namun, umbi ini kurang dimanfaatkan karena adanya senyawa antinutrisi, yaitu cyanogenic glucosides. Penghilangan senyawa sianida dari bahan makanan dapat dilakukan baik dengan metode fisik, kimia atau biologi. Penelitian ini bertujuan untuk mengkaji pengaruh waktu pada detoksifikasi irisan umbi gadung dari senyawa sianida melalui fermentasi dengan menggunakan kapang Mucor racemosus dan kinetikanya. Cuplikan irisan umbi gadung diambil dari sistem fermentasi pada setiap jeda waktu 24 jam untuk dianalisis kadar biomassa dan sianidanya. Hasil penelitian menunjukkan bahwa kandungan sianida menurun seiring dengan bertambahnya waktu fermentasi. Irisan umbi gadung yang aman dikonsumsi dapat diperoleh dari fermentasi irisan ubi gadung selama 120 jam dengan kandungan sianida serendah 49,41 mg/kg. Persamaan logistik berhasil menggambarkan tingkat pertumbuhan kapang Mucor racemosus dengan baik. Tingkat pertumbuhan spesifik dari Mucor racemosus pada irisan umbi gadung adalah 0,0297/jam atau sekitar setengah dari laju pertumbuhan spesifik jamur tersebut saat dibudidayakan pada media yang siap dikonsumsi, seperti ragi-pepton-glukosa (YPG).


Kata kunci: fermentasi; gadung; monod; Mucor racemosus; sianogen 


fermentasi; gadung; monod; Mucor racemosus; sianogen

  1. marissa widiyanti 
    Program Studi Magister Teknik Kimia, Departemen Teknik Kimia, Fakultas Teknik, Universitas Diponegoro Jl. Prof. Soedarto, SH Tembalang Semarang 50275 Telp./Fax. (024)7460058/(024)76480675
  2. Andri Cahyo Kumoro 
    Departemen Teknik Kimia, Fakultas Teknik, Universitas Diponegoro Jl. Prof. Soedarto, SH Tembalang Semarang 50275 Telp./Fax. (024)7460058/(024)76480675

Alves, M.H., Galba, M., and Kaoru, O., (2005), Detection of Extracellular Protease in Mucor Species, Revista Iberoamericana de Micologia, 22, pp.114-117.

AOAC, 1990, Official Methods of Analysis, 15th, Washington, D.C.

Araujo, T.R., Petkowicz, C.L.O., Cardoso, V.L., Filho, U.C., and Vieira, P.A., (2016), Biopolymer Production Using Fungus Mucor racemosus Fresenius and Glycerol as Substrate, Polimeros, 26(2), pp. 144-151.

Aynseley, M., Ward, A.C., and Wright, A.R., (1990), A Mathematical Model for the Growth of Mycelial Fungi in Submerged Culture, Biotechnology and Bioengineering, 35, pp. 820-830.

Balmant, W., Sugai-Guérios, M.H., Coradin, J.H., Krieger, N., Junior, A.F., and Mitchell, D.A., (2015), A Model for Growth of a Single Fungal Hypha Based on Well-Mixed Tanks in Series: Simulation of Nutrient and Vesicle Transport in Aerial Reproductive Hyphae, Plos One, DOI:10.1371/ journal.pone.0120307, pp. 1-22.

Bhatia, Y., Mishra, S., and Bisaria, V.S., (2002), Microbial β-glucosidases: Cloning, Properties, and Applications. Critical Reviews in Biotechnology, 22, pp. 375-407.

Bredenkamp, A., Velankar, H., van Zyl, W.H., and G¨orgens, J.F., (2010), Effect of Dimorphic Regulation on Heterologous Glucose Oxidase Production by Mucor Circinelloides, Yeast, 27, pp. 849-860.

Borgia, P. and Sypherd, P.S., (1977), Control of β-glucosidase Synthesis in Mucor racemosus, Journal of Bacteriology, 130, pp. 812-817.

Bradbury, J.H., (1991), Properties and Analysis of Antinutritional Factors in Foods, ASEAN Food Journal, 6(4), pp.123-128.

Chen, Z.S. and Voregen, A.G.J.,(2004), Differently Sized Granules From Acetylated Potato and Sweet Potato Starches Differ in the Acetyl Substitution Pattern of their Amylase Populations, Carbohydrate Polymer, 56, pp. 219-226.

Damardjati, D.S., Widowati, dan Suismono, (1993), Pembangunan Sistem Agroindustri Kassava, Balai Penelitian Pertanian, Sukamandi.

Dantigny, P., Mansur, C.S., Sautour, M., Tchobanov, I., and Bensoussan, M., (2002), Relationship Between Spore Germination Kinetics and Lag Time during Growth of Mucor racemosus, Letters in Applied Microbiology, 35, pp. 395-398.

Hardjo, M., (2005), Tepung Gadung (Dioscorea Hispida Dennst) Bebas Sianida dengan Merendam Parutan Umbi dalam Larutan Garam, Jurnal Matematika, Sains, dan Teknologi, 6(2), hal. 92-99.

Harijono, Sari, T.A., dan Martati, E., (2008), Detoksifikasi Umbi Gadung (Dioscorea hispida Dennst.) dengan Pemanasan Terbatas dalam Pengolahan Tepung Gadung, Jurnal Teknologi Pertanian, 9(2), hal. 75-82.

Hikino, H., Cono, C., Takahashi, M., Murakami, M., Kato, Y., Karikura, M. and Hayashi, T.,(1986), Isolation and Hypoglycemic Activity of Dioscorans A, B, C, D, E, and F; Glycans of Dioscorea Japonica Rhizophors, Planta Medica, 3, pp.168-171.

Hudzari, R.M., Ssomad, M.A.H.A., Rizuwan, Y.M., Asimi, M.N.N., Abdullah, A.B.C., and Fauzan, M.Z.M., (2011), Modification of Automatic Alkaloid Rem Oval System for Dioscorine, International Journal of Agronomy & Plant Production, 2, pp.155-161.

Jakovljević, V.D., Milićević, J.M., Stojanović, J.D., and Vrvić, M.M., (2014), The Ability of Fungus Mucor racemosus Fresenius to Degrade High Concentration of Detergent, Chemical Industry & Chemical Engineering Quarterly, 20 (4), pp. 587-595.

Ketiku, A.O., Akinyele, I.O., Kenshiro, O., and Akinnowo, O., (1978), Changes in the Hydrocianic Acid Concentration during Traditional Processing of Cassava into Gari and Iafun, Food Chemistry, 3, pp. 221-228.

Kumoro, A.C., Retnowati, D.S.,and Budiyati, C.S., (2011), Removal of Cyanides From Gadung (Dioscorea Hispida Dennst.) Tuber Chips Using Leaching and Steaming Techniques, Journal of Applied Sciences Research, 7(12), pp. 2140-2146.

Kumoro, A.C., Ratnawati, R., Retnowati, D.S., and Budiyati, C.S., (2016), Microbial Detoxification of Gadung (Dioscorea Hispida Dennst) Tuber: Effect of Microbes Loading and Time, Proceeding of the 2nd International Conference of Mathematics and Natural Sciences, Bandung. Paper No. LSC-307, pp. 1-6.

L¨ubbeh¨usen, T.L., Nielsen, J., and McIntyre, M., (2004), Aerobic and Anaerobic Ethanol Production by Mucor Circinelloides during Submerged Growth, Applied Microbiology and Biotechnology, 63, pp. 543-548.

Mitchell, D.A., von Meien, O.F., Krieger, N., and Dalsenter, F.D.H., (2004), A Review of Recent Developments in Modeling of Microbial Growth Kinetics and Intraparticle Phenomena in Solid-State Fermentation, Biochemical Engineering Journal, 17, pp. 15-26.

Myoda, T., (2006), Identification of Soluble Proteins and Interaction with Mannan in Mucilage of Dioscorea Opposita Thunb.(Chinese Yam Tuber), Food Science and Technology Research, 12(4), pp. 299-302.

Okolie, N.P. and Ugochukwu, E.N., (1988), Changes in Activities of Cell-Wall-Degrading Enzymes during Fermentation of Cassava (Manihot esculentu Crantz) with Citrobacter freundii, Journal of the Science of Food and Agriculture, 44, pp. 51-61.

Orlowski, M., (1981), Growth Rate Dependent Adjustment of Ribosom Function I the Fungus Mucor racemosus, Journal of Biochemistry, 196, pp.403-410.

Sasongko, P., (2009), Detoksifikasi Umbi Gadung (Dioscorea Hispida Dennst.) Proses Fermentasi Menggunakan Kapang Mucor racemosus, Jurnal Teknologi Pertanian, 10(3), hal. 205-215.

Setyowati, F.M. dan Siagian, (2004), Pemanfaatan Tumbuhan Pangan oleh Masyarakat Talang Mamak di Taman Nasional Bukit Tiga Puluh, Jambi, Biota Jurnal Ilmiah Ilmu-ilmu Hayati, IX(1), hal. 11-18.

Shewry, P.R., (2003), Tuber Storage Proteins, Annals of Botany, 91(7), pp. 755-769.

Spier, M.R., Letti, L.A.J., Woiciechowski, A.L., and Soccol, C.R., (2009), A Simplified Model for A. Niger FS3 Growth during Phytase Formation in Solid State Fermentation, Brazilian Archives in Biology and Technology, 52, pp. 151-158.

Suliantari dan Rahayu, P.W., (1990), Teknologi Fermentasi Biji dan Umbi-Umbian, PAU Pangan dan Gizi IPB, Bogor.

Sunarsih, E.S., Djatmika, and Utomo, R.S., (2007), Influence of Administration of Gadung Corm (Dioscorea hispida Dennst) Infusion to Decrease of Blood Glucose Level at Aloksan Inducted Male Diabetic Rats, Majalah Farmasi Indonesia, 18(1), pp. 29-33.

Takó, M., Farkas, E., Lung, S., Krisch, J., Vágvölgyi, C., and Papp, T., (2010), Identification of Acid- and Thermotolerant Extracellular β-glucosidase Activities in Zygomycetes Fungi, Acta Biologica Hungarica, 61, pp.101-110.

Tatiyakul, J., Naksriarporn, T., and Pradipasena P., (2012), X-ray Diffraction Pattern and Functional Properties of Dioscorea hispida Dennst Starch Hydrothermally Modified at Different Temperatures, Food and Bioprocess Technology, 5, pp. 964-971.

Webster, J., Beck, W., and Ternai, B.,(1984), Toxicity and Bitterness in Australian Dioscorea bulbifera L. and Dioscorea hispida Dennst. from Thailand, Jornal Agriculture Food Chemistry, 32, pp.1087-1090.

Wetsby, A. and Twiddy, R., (1992), Characterization of Gari and Fu-fu Preparation Procedures in Nigeria, World Journal of Microbiology and Biotechnology, 8(2), pp.175-182.