DOI: https://doi.org/10.14710/buloma.v%25vi%25i.3802

Cultivation of Chlorella sp. as Biofuel Sources in Palm Oil Mill Effluent (POME)

Department of Chemical Engineering, Faculty of Engineering, Diponegoro University, Indonesia

Published: 1 Jul 2012.
Editor(s): H. Hadiyanto

Citation Format:
Abstract
Renewable energy is essential and vital aspect for development in Indonesia especially less oil reserve for coming 15 years. Biodiesel has received much attention as renewable energy in recent years. One of potential biodiesel is produced from microalgae. Due to high content of nutrients in Palm Oil Mill Effluent (POME), this waste is a potential for nutrient growth for microalgae. Chlorella is one of high potential for biodiesel since it has high lipid content (20-30%). The objective of the research is to determine growth rate and biomass productivity in Chlorella Sp cultured in POME. Chlorella Sp was cultured in 20%, 50%, 70% POME using urea concentration 0.1gr/L (low nitrogen source) and 1gr/l (high nitrogen source) at flask disk, pH 6.8-7.2; aerated using aquarium pump and fluorescence lamp 3000-6000 lux as light. Medium was measured using spectrophotometer Optima Sp-300 OD at 680 wave length in 15 days to calculate specific growth rate. At end of cultivation, Chlorella sp was filtered and measured as dry weight. Result indicated that Chlorella sp at 50% POME 1gr/L urea showed higher specific growth rate (0.066/day). Factor affecting growth rate of microalgae is CNP ratio, POME concentration, and urea concentration.
Fulltext View|Download

Article Metrics:

Article Info
Section: Original Research Article
Language : EN
Recent articles
Potency of Solar Energy Applications in Indonesia Biogas Production from Cow Manure Thermodynamic Model of a Very High Efficiency Power Plant based on a Biomass Gasifier, SOFCs, and a Gas Turbine More recent articles
Most cited articles
Study of Gasohol as Alternative Fuel for Gasoline Substitution: Characteristics and Performances Effects on NOx and SO2 Emissions during Co-Firing of Coal With Woody Biomass in Air Staging and Reburning Multi-Criteria Decision Making (MCDM) Approach for Selecting Solar Plants Site and Technology: A Review Experimental and Numerical Investigation of Nanofluid Usage in a Plate Heat Exchanger for Performance Improvement Thermal Energy Optimization of Building Integrated Semi-Transparent Photovoltaic Thermal Systems More cited articles
  1. Rupani PF, Singh RP, Ibrahim MH, Norizan E (2010) Review of Current Palm Oil Mill Effluent (POME) Treatment Methods:Vermicomposting as a Sustainable Practice. World Applied Sciences Journal 11: 70-81
  2. Deptan (2009) Rancangan Rencana Strategis Kementrian Pertanian Tahun 2010-2014. www.deptan.go.id
  3. Chisti Y (2007) Biodiesel from microalgae. Biotechnology Advances 25(3): 294-306. https://doi.org/10.1016/j.biotechadv.2007.02.001
  4. Oh HM, Lee SJ, Park MH (2001) Harvesting of Chlorella vulgaris using A Bioflocculant from Paenibacillus sp. AM49. Biotechnol. Lett. 23: 1229-34
  5. Puangbut M, Leesing R (2012) Integrated Cultivation Technique for Microbial Lipid Production by Photosynthetic Microalgae and Locally Oleaginous Yeast. World Academy of Science, Engineering and Technology 64
  6. Putri EV, Din MFM, Ahmed Z, Jamaluddin H, Chelliapan S (2011) Investigation of Microalgae for High Lipid Content using Palm Oil Mill effluent (POME) as Carbon Source.International Conference on Environment and Industrial Innovation. IPCBEE vol.12. LACSIT Press Singapore
  7. Habib MAB, Yusoff FM, Phang SM, Kamarudin MS, Mohamed S (1998) Chemical Characteristics and Essential Nutrients of Agro Industrial Effluents in Malaysia. Asian Fisheries Science 11(3): 279-286
  8. Habib MAB, Yusoff FM, Phang SM, Kamarudin MS, Mohamed S (2003) Growth and Nutritional Values of Molina micrura Fed on Chlorella vulgaris Grown in Digested Palm Oil Mill Effluent. Asian Fisheries Science 16: 107-119
  9. Edwards P, Sinchumpasak OA, Ouano EAO (1980) A Study of A Sewage Fed Highrate Stabilization Pond in Thailand. Wastewater and Resources Recovery (IDRC-15e). International Development Research Centre, Ottawa, Canada. p. 42
  10. Phalakornkule C, Mangmeemak J, Intrachod BN (2010) Pretreatment of Palm Oil Mill Effluent by Electrocoagulation and Coagulation. ScienceAsia 36: 142-149. https://doi.org/10.2306/scienceasia1513-1874.2010.36.142
  11. El-Sayed AB, Abdel-Maguid AA, Hoballah EM (2011) Growth Response of Chlorella vulgaris to Acetate Carbon and Nitrogen Forms. Nature and Science 9: http://www.sciencepub.net
  12. Choochote W, Paiboonsin K, Ruangpan, S, Pharuang, A (2010) Effects of Urea and Light Intensity on the Growth of Chlorella sp. The 8th International Symposium on Biocontrol and Biotechnology. http://www.science.kmitl.ac.th/downloads/proceeding_2/22%20page127-134.pdf
  13. Mandalam RK, Palsson BO (1998) Elemental Balancing of Biomass and Medium Composition Enhances Growth Capacity in High-Density Chlorella vulgaris Cultures. Biotechnol. Bioeng. 59: 605-611. https://doi.org/10.1002/(SICI)1097-0290(19980905)59:5<605::AID-BIT11>3.0.CO;2-8
  14. Wijanarko A (2009) Effect of presence of Substituted Urea and also Ammonia as Nitrogen Source in Cultivated Medium on Chlorella's Lipid Content. Progress in Biomass and Bioenergy Production. ISBN 978-953-307-491-7, Intech. Ch.14
  15. Agwa OK, Ibe SN, Abu GO (2012) Economically Effective Potential of Chlorella sp. for Biomass and Lipid Production. Journal of Microbiology and Biotechnology Research 2(1): 35-45
  16. Cheirsilp B, Torpee S (2012) Enhanced Growth and Lipid Productivity of Microalgae under Mixotrophic Culture Condition: Effect of Light Intensity, Glucose Concentration and Fed-Batch Cultivation. Bio resource Technology 110: 510-516. https://doi.org/10.1016/j.biortech.2012.01.125
  17. Widjaja A, Chien CC, Ju, YH (2009) Study of Increasing Lipid Production from Fresh Water Microalgae Chlorella vulgaris. Journal of the Taiwan Institute of Chemical Engineers 40: 13-20. https://doi.org/10.1016/j.jtice.2008.07.007
  18. Hadiyanto, H., Widayat, W., & Kumoro, A. (2012). Potency of Microalgae as Biodiesel Source in Indonesia. International Journal Of Renewable Energy Development (IJRED), 1(1), 23-27. https://doi.org/10.14710/ijred.1.1.23-27

Last update:

  1. Effects of nutrient ratios and carbon dioxide bio-sequestration on biomass growth of Chlorella sp. in bubble column photobioreactor

    Hoang-Nhat-Phong Vo, Xuan-Thanh Bui, Thanh-Tin Nguyen, Dinh Duc Nguyen, Thanh-Son Dao, Ngoc-Dan-Thanh Cao, Thi-Kim-Quyen Vo. Journal of Environmental Management, 219 , 2018. doi: 10.1016/j.jenvman.2018.04.109

  2. Sustainable microalgae-based palm oil mill effluent treatment process with simultaneous biomass production

    Fatimah T. A. Halim, Xiaoyi Guo, Gaomin Su, Hannah L. Ngee, Xianhai Zeng, Ning He, Lu Lin, Michael K. Danquah. The Canadian Journal of Chemical Engineering, 94 (10), 2016. doi: 10.1002/cjce.22584

  3. Processing technology POME-pond in Indonesia: A mini review

    D Leela, S M. Nur. IOP Conference Series: Earth and Environmental Science, 365 , 2019. doi: 10.1088/1755-1315/365/1/012009

  4. Palm Oil

    Hesam Kamyab, Shreeshivadasan Chelliapan, Mohd Fadhil Md Din, Shahabaldin Rezania, Tayebeh Khademi, Ashok Kumar. 2018. doi: 10.5772/intechopen.75811

  5. Utilizing Palm Oil Mill Waste for Co-Production of Biogas and Microalgae Biomass

    H. Hadiyanto, M. Muslihuddin, E Sudirjo, N Padetya. IOP Conference Series: Earth and Environmental Science, 248 , 2019. doi: 10.1088/1755-1315/248/1/012073

  6. Integration of bioelectricity generation from algal biophotovoltaic (BPV) devices with remediation of palm oil mill effluent (POME) as substrate for algal growth

    Fong-Lee Ng, Siew-Moi Phang, Cheng-Han Thong, Vengadesh Periasamy, Jeccy Pindah, Kamran Yunus, Adrian C. Fisher. Environmental Technology & Innovation, 21 , 2021. doi: 10.1016/j.eti.2020.101280

  7. Evaluation of processing route alternatives for accessing the integration of algae-based biorefinery with palm oil mill

    Nur Nabila Abdul Hamid, Jeng Shiun Lim. Journal of Cleaner Production, 212 , 2019. doi: 10.1016/j.jclepro.2018.12.104

  8. Cultivation of Chlorella vulgaris using different sources of carbon and its impact on lipid production

    Yunus Fransiscus, Edy Purwanto. AIP Conference Proceedings, 1840 , 2017. doi: 10.1063/1.4982265

  9. Electrogenic and biomass production capabilities of a Microalgae–Microbial fuel cell (MMFC) system using tapioca wastewater and Spirulina platensis for COD reduction

    H. Hadiyanto, Marcelinus Christwardana, Carlito da Costa. Energy Sources, Part A: Recovery, Utilization, and Environmental Effects, 45 (2), 2023. doi: 10.1080/15567036.2019.1668085

  10. Review of microalgae growth in palm oil mill effluent for lipid production

    Rosnani Resdi, Jeng Shiun Lim, Hesam Kamyab, Chew Tin Lee, Haslenda Hashim, Nadzirah Mohamad, Wai Shin Ho. Clean Technologies and Environmental Policy, 18 (8), 2016. doi: 10.1007/s10098-016-1204-1

  11. Microalgae Cultivation in Palm Oil Mill Effluent (POME) Treatment and Biofuel Production

    Sze Shin Low, Kien Xiang Bong, Muhammad Mubashir, Chin Kui Cheng, Man Kee Lam, Jun Wei Lim, Yeek Chia Ho, Keat Teong Lee, Heli Siti Halimatul Munawaroh, Pau Loke Show. Sustainability, 13 (6), 2021. doi: 10.3390/su13063247

  12. Opportunities and Challenges of Microalgal Cultivation on Wastewater, with Special Focus on Palm Oil Mill Effluent and the Production of High Value Compounds

    Muhamad Maulana Azimatun Nur, Anita G. J. Buma. Waste and Biomass Valorization, 10 (8), 2019. doi: 10.1007/s12649-018-0256-3

  13. Treatment and Valorization of Palm Oil Mill Effluent through Production of Food Grade Yeast Biomass

    Joy O. Iwuagwu, J. Obeta Ugwuanyi. Journal of Waste Management, 2014 , 2014. doi: 10.1155/2014/439071

  14. Rubber wastewater treatment using UV, ozone, and UV/ozone and its effluent potency for microalgae Spirulina platensis cultivation medium

    Hadiyanto Hadiyanto, Marcelinus Christwardana, Detian Indah Pratiwi, Silviana Silviana, Muhammad Syarifudin, Adian Khoironi, Sanjay Kumar Shukla. Cogent Engineering, 7 (1), 2020. doi: 10.1080/23311916.2020.1797980

  15. The Effects of Audible Sound for Enhancing the Growth Rate of Microalgae Haematococcus pluvialis in Vegetative Stage

    Marcelinus Christwardana, H. Hadiyanto. HAYATI Journal of Biosciences, 24 (3), 2017. doi: 10.1016/j.hjb.2017.08.009

Last update: 2024-12-23 18:36:45

  1. Comparative analysis between pyrolysis products of Spirulina platensis biomass and its residues

    Jamilatun S.. International Journal of Renewable Energy Development, 8 (2), 2019. doi: 10.14710/ijred.8.2.133-140

  2. Effects of nutrient ratios and carbon dioxide bio-sequestration on biomass growth of Chlorella sp. in bubble column photobioreactor

    Hoang-Nhat-Phong Vo, Xuan-Thanh Bui, Thanh-Tin Nguyen, Dinh Duc Nguyen, Thanh-Son Dao, Ngoc-Dan-Thanh Cao, Thi-Kim-Quyen Vo. Journal of Environmental Management, 219 , 2018. doi: 10.1016/j.jenvman.2018.04.109

  3. Sustainable microalgae-based palm oil mill effluent treatment process with simultaneous biomass production

    Fatimah T. A. Halim, Xiaoyi Guo, Gaomin Su, Hannah L. Ngee, Xianhai Zeng, Ning He, Lu Lin, Michael K. Danquah. The Canadian Journal of Chemical Engineering, 94 (10), 2016. doi: 10.1002/cjce.22584

  4. Processing technology POME-pond in Indonesia: A mini review

    D Leela, S M. Nur. IOP Conference Series: Earth and Environmental Science, 365 , 2019. doi: 10.1088/1755-1315/365/1/012009

  5. Utilizing Palm Oil Mill Waste for Co-Production of Biogas and Microalgae Biomass

    H. Hadiyanto, M. Muslihuddin, E Sudirjo, N Padetya. IOP Conference Series: Earth and Environmental Science, 248 , 2019. doi: 10.1088/1755-1315/248/1/012073

  6. Effects of Yeast Concentration and Microalgal Species on Improving the Performance of Microalgal-Microbial Fuel Cells (MMFCs)

    Hadiyanto H.. International Energy Journal, 20 (3), 2020.

  7. Integration of bioelectricity generation from algal biophotovoltaic (BPV) devices with remediation of palm oil mill effluent (POME) as substrate for algal growth

    Fong-Lee Ng, Siew-Moi Phang, Cheng-Han Thong, Vengadesh Periasamy, Jeccy Pindah, Kamran Yunus, Adrian C. Fisher. Environmental Technology & Innovation, 21 , 2021. doi: 10.1016/j.eti.2020.101280

  8. Evaluation of processing route alternatives for accessing the integration of algae-based biorefinery with palm oil mill

    Nur Nabila Abdul Hamid, Jeng Shiun Lim. Journal of Cleaner Production, 212 , 2019. doi: 10.1016/j.jclepro.2018.12.104

  9. Cultivation of Chlorella vulgaris using different sources of carbon and its impact on lipid production

    Yunus Fransiscus, Edy Purwanto. AIP Conference Proceedings, 1840 , 2017. doi: 10.1063/1.4982265

  10. Electrogenic and biomass production capabilities of a Microalgae–Microbial fuel cell (MMFC) system using tapioca wastewater and Spirulina platensis for COD reduction

    H. Hadiyanto, Marcelinus Christwardana, Carlito da Costa. Energy Sources, Part A: Recovery, Utilization, and Environmental Effects, 45 (2), 2023. doi: 10.1080/15567036.2019.1668085

  11. Review of microalgae growth in palm oil mill effluent for lipid production

    Rosnani Resdi, Jeng Shiun Lim, Hesam Kamyab, Chew Tin Lee, Haslenda Hashim, Nadzirah Mohamad, Wai Shin Ho. Clean Technologies and Environmental Policy, 18 (8), 2016. doi: 10.1007/s10098-016-1204-1

  12. Phytoremediations of Palm Oil Mill Effluent (POME) by using aquatic plants and microalge for biomass production

    Hadiyanto . Journal of Environmental Science and Technology, 6 (2), 2013. doi: 10.3923/jest.2013.79.90

  13. Opportunities and Challenges of Microalgal Cultivation on Wastewater, with Special Focus on Palm Oil Mill Effluent and the Production of High Value Compounds

    Muhamad Maulana Azimatun Nur, Anita G. J. Buma. Waste and Biomass Valorization, 10 (8), 2019. doi: 10.1007/s12649-018-0256-3

  14. Palm oil mill effluent as algae cultivation medium for biodiesel production

    Idris N.A.. Journal of Oil Palm Research, 30 (1), 2018. doi: 10.21894/jopr.2018.0011

  15. Renewable energy from the seaweed chlorella pyrenoidosa cultivated in developed systems

    Zighmi S.. International Journal of Renewable Energy Research, 7 (1), 2017.

  16. Rubber wastewater treatment using UV, ozone, and UV/ozone and its effluent potency for microalgae Spirulina platensis cultivation medium

    Hadiyanto Hadiyanto, Marcelinus Christwardana, Detian Indah Pratiwi, Silviana Silviana, Muhammad Syarifudin, Adian Khoironi, Sanjay Kumar Shukla. Cogent Engineering, 7 (1), 2020. doi: 10.1080/23311916.2020.1797980

  17. The Effects of Audible Sound for Enhancing the Growth Rate of Microalgae Haematococcus pluvialis in Vegetative Stage

    Marcelinus Christwardana, H. Hadiyanto. HAYATI Journal of Biosciences, 24 (3), 2017. doi: 10.1016/j.hjb.2017.08.009