DOI: https://doi.org/10.14710/jai.v8i1.11860

Pengaruh Lidokain Intravena Terhadap Kadar Superoxide Dismutase I (SOD1) Dan Gambaran Histopatologi Jantung Kelinci Pada Myocardial Ischemic Reperfusion Injury Model

*Haris Lutfi  -  Rumah Sakit Keluarga Sehat, Margorejo, Indonesia
Widya Istanto  -  Bagian Anestesi dan Terapi Intensif FK Universitas Diponegoro/ RSUP Dr. Kariadi , Indonesia
Mohammad Sofyan Harahap  -  Bagian Anestesi dan Terapi Intensif FK Universitas Diponegoro/ RSUP Dr. Kariadi , Indonesia

Citation Format:
Abstract

Latar Belakang: Ischemic reperfusion injury (IRI) adalah kerusakan jaringan yang disebabkan oleh kembalinya pasokan darah ke jaringan setelah periode iskemia atau kekurangan oksigen. Salah satu pilihan terapi untuk mencegah cedera jaringan adalah dengan meningkatkan antioksidan alami yang berada di dalam sel seperti superoxide dismutase (SOD).

Tujuan : Membuktikan pengaruh lidokain intravena terhadap kadar Superoxide Dismutase 1 (SOD J) dan gambaran histopatologi jantung kelinci pada myocardial ischemic reperfusion injury model.

Metode : Penelitian menggunakan 16 ekor kelinci lokal jantan, berumur 1 - 2 tahun, berat badan 1.5 - 2,5 kg. Penelitian menggunakan 2 kelompok yaitu kelompok kontrol (KK) terdiri dari 8 ekor kelinci yang diberikan perlakuan myocardial ischemic reperfusion injury. Kelompok perlakuan (KP) terdiri 8 ekor kelinci mendapatkan perlakuan myocardial ischemic reperfusion injury dan diberikan lidokain 2% i.v dengan dosis 1.5 mg/kg/jam secara kontin>ii dari awal sampai akhir prosedur. Kadar SOD1 diperiksa dengan metode EL1SA sedangkan histopatologi jaringan diperiksa menggunakan mikroskop kemudian hasilnya dihitung dengan persamaan regresi linier.

Hasil : Kadar SOD1 didapatkan nilai p = 0,009. karena p < 0.05 maka dapat disimpulkan terdapat perbedaan bermakna antara kelompok kontrol dan perlakuan. Untuk gambaran histopatologi didapatkan nilai p = 0,323, karena p >0,05 maka dapat disimpulkan terdapat perbedaan tidak bermakna antara kelompok kontrol dan perlakuan.

Simpulan : Terdapat perbedaan bermakna kadar SOD1 jantung kelinci pada myocardial ischemic reperfusion injury yang diberikan lidokain dibandingkan dengan kelinci yang tidak mendapat lidokain. Sedangkan untuk gambaran histopatologi terdapat perbedaan tidak bermakna pada kelinci myocardial ischemic reperfusion injwy yang diberikan lidokain dibandingkan dengan kelinci yang tidak mendapat lidokain.

Fulltext View|Download
Keywords: Ischemic reperfusion injury (IRI); Lidokain; Superoxide Dismutase 1 (SODI)

Article Metrics:

Article Info
Section: Penelitian
Language : ID
Recent articles
Pengaruh Lidokain Intravena Terhadap Kadar Superoxide Dismutase I (SOD1) Dan Gambaran Histopatologi Jantung Kelinci Pada Myocardial Ischemic Reperfusion Injury Model Pengaruh Pemberian Ropivakain Infiltrasi Terhadap Tampilan Kolagen Di Sekitar Luka Insisi Pada Tikus Wistar Perbandingan Respon Hemodinamik 3 Menit dan 5 Menit Setelah Pemberian Fentanyl 2 mcg/kgbb iv saat Intubasi di RSUD DR Saiful Anwar Malang Kaudal Epidural Kontinyu Pada Pasien Pediatri yang Menjalani Pembedahan Abdomen dan Rectum. Pengukuran CVC pada pasien Sepsis, Apakah terdapat keuntungan? More recent articles
  1. De Backer D, Dubois MJ, Schmartz D, Koch M, Ducart A. Barvais L et al. Microcirculatory Alterations in Cardiac Surgery: Effects of Cardiopulmonaiy Bypass and Anesthesia. Ann Thorac Surg. 2009; 88: 1396-403
  2. Marcelo L, Moraes L, Auler C, Carmona C. Myocardial Protection in Cardiac Surgery: Review Article. Rev. Bras. Anestesiol. 2005; 55(5): 565-574
  3. Hausenloy DL Yellon DM. Myocardial ischemia-reperfusion injwy: a neglected therapeutic target. J Clin Invest. 2013; 123(1): 92-100
  4. Sanada S. Komuro L Kitakaze M. Pathophysiology of Myocardial Reperfusion Injury: Preconditioning, Postconditioning, and Translational Aspects of Protective Measures. Am J Physiol Heart Circ Physiol. 2011: 301(5): 1723-1741
  5. Auerbach AD. Goldman L. Beta-B lockers and Reduction of Cardiac Events in Non Cardiac Surgeiy: Scientific Review. JAMA. 2002; 287(11) : 1435-1444
  6. Wada A. Myocardial Ischemia in The Postanesthesia Care Unit: A Case Report. AANA Journal. 2007: 75(1) : 34-36
  7. FraBdorf J. De Hert S. Schiack W. Anaesthesia and myocardial ischaemia/reperfusion injwy. Br J Anaesth. 2009; 103(1): 89-98
  8. Imaizumi U, Furuya M, Itakura S, Yui H, Tagawa T, Sakuraba S et al. Effects of Lidocaine on Ischemia /Reperfusion Injury in In vivo Rabbit Hearts. J Anesth Clin Res. 2012; 3(11): 261
  9. Droge W. Physiological Reviews: Free Radicals in the Physiological Control of Cell Function. Physiol Rev. 2002; 82(1): 47-95
  10. Eroglu A. The Effect of Intravenous Anesthetics on Ischemia-Reperfusion Injury. Bio Med Res Int. 2014 Jan; 821-513
  11. Ebel D, Limfert P, FraBdorf J, Preckel B, Mullenheim J, Thamer V et al. Lidocaine Reduces Ischaemic but Not Reperfusion Injury in Isolated Rat Heart. Br J Anaesth. 2001; 86(6): 846-852
  12. Kaczmarek DJ, Herzog C, Larmann J, Gillmann HJ, Hiidebrand R, Schinitz M et al. Lidocaine Protects from Myocardial Damage due to Ischemia and Reperfusion in Mice by Its Antiapoptotic Effects. Anesthesiology. 2009; 110: 1041-9
  13. Yamabayashi K, Momota Y. Miyamae M, Kaneda K. and Kotani J. Lidocaine Protects Against Myocardial Ischemia-Reperfusion Injury in Anesthetized Rabbits. J Osaka Dent Univ. 2013: 47(2): 201-207
  14. Bonow RO, Mann DL. Zipes DP, Libby P. Atherosclerotic Cardiovascular Disease: Coronary Blood Flow and Myocardial Ischemia; Metabolic and Functional Concequences of Ischemia. In: Braunwald's Heart Disease A Textbook of Cardiovascular Medicine 9th ed. Philadelphia; Elsevier Saunders. 2012: 1066-1067
  15. Kloner RA, Jennings RB. Consequences of Brief Ischemia: Stunning, Preconditioning, and Their Clinical Implications: Part 1. Circulation. 2001; 104:2981-2989
  16. Walters AM, Porter GA, Brookes PS. Mitochondria as a Drug Target in Ischemic Heart Disease and Cardiomyopathy. Circ Res. 2012; 111: 1222-1236
  17. Ondiveeran HK, Robichaud AF. New Developments in The Treatment of Ischemia / Reperfusion Injury. Current Opinion in Investigational Drugs. 2001; 2(6): 783-791
  18. Turer AT, Hill JA. Pathogenesis of Myocardial Ischemia-Reperfusion Injury and Rationale for Therapy. Am J Cardiol. 2010; 106(3): 360-368
  19. Bandyopadhyay U, Das D. Banerjee RK. Reactive Oxygen Species: Oxidative Damageand Pathogenesis. Current Science. 1999; 77(5): 658-664
  20. Weydert CJ, Cullen JJ. Measurement Of Superoxide Dismutase, Catalase, And Glutathione Peroxidase In Cultured Cells And Tissue. Nat Protoc. 2010; 5(1): 51-66
  21. Danielisova V, Nemethova M, Gottlieb M, Burda J. Changes of Endogenous Antioxidant Enzymes During Ischemic Tolerance Acquisition. Neurochem Res. 2005; 30(4): 559-563
  22. Almaraz CC, Pinilla PJG. Pozo MJ, Camello PJ. Cell Physiology: Mitochondrial Reactive Oxygen Species and Ca TSignaling. Am J Physiol. 2006; 291: 1082-1088
  23. Buck ML. Use of Lidocaine for Analgesia in Children and Adolescents. PediatrPharm,2013; 19(12)
  24. Sheets MF, Hanck DA. Molecular Action of Lidocaine on the Voltage Sensors of Sodium Channels. J Gen Physiol. 2003; 121(2): 163-175
  25. Aldakkak M, Stowe DF, Lesnefsky EJ, Heisner JS, Chen Q, Camara AKS. Modulation of Mitochondrial Bioenergetics in The Isolated Guinea Pig Beating Heart by Potassium and Lidocaine Cardioplegia: Implications for Cardioprotection. J Cardiovasc Pharmacol. 2009; 54(4): 298-309
  26. Unal Y, Iriz E, Yilmazer D, Kavutcu M, Alper M, Kurtipek O et al. Effects of Lidocaineon Membrane Stabilization in Harvested Vein Graft Storage. Saudi Med J. 2009; 30(2): 203 - 208
  27. Lee EH, Lee HM, Chung CH, Chin JH, Choi DK. Chung HJ et al. Impact of intravenous lidocaine on myocardial injwy after off-pump coronary artery surgeiy. BJA, 2011; 106(4): 487-493
  28. Arakawa K, Takahashi H. Nakagawa S, Ogawa S. The Effects of Lidocaine on Superoxide Production and p47 Phox Translocation in Opsonized Zymosan-Activated Neutrophils. Anesth Analg, 2001; 93: 1501-1506
  29. Verma S, Fedak PWM, Weisel RD, Butany J, Rao V, Maitland A et al. Clinician update: Fundamentals of Reperfusion Injwy for the Clinical Cardiologist. Circulation, 2002: 105: 2332-2336

Last update:

No citation recorded.

Last update:

No citation recorded.