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Effects of mixture powder of black rice (Oryza sativa L indica), red beans (Phaseolus vulgaris L), and moringa leaves (Moringa oleifera L) on blood glucose concentration in hyperglycemic Rats

*Lalu Juntra Utama  -  Jurusan Gizi Poltekkes Kemenkes Kupang, Indonesia
Suryana Suryana  -  Jurusan Gizi Poltekkes Kemenkes Aceh, Indonesia
Anita Christina Sembiring  -  Jurusan Gizi Poltekkes Kemenkes Kupang, Indonesia
Received: 19 Jun 2020; Published: 30 Jun 2021.

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Abstract

Background: Diabetes mellitus, increased blood glucose or hyperglycemia, is associated with increased oxidative stress and cardiovascular diseases. This condition will further cause carbohydrate and fat metabolism change, resulting in the decreased antioxidant defense system. Black rice, red beans, and moringa leaves contain oleic acid, butyric amino acid, antioxidants, phytic acid, and arginine, which can improve insulin sensitivity, and blood glucose homeostasis.

Objective: This study aimed to analyze the effect of betamelor (black rice, red beans, and moringa leaves) on blood glucose in rats

Materials and Methods: The design of this research was experimental research with pre-and post-control group design. A total of 20 Sprague Dawley female rats were divided into four groups, namely standard feed (PS), 80% feed of betamelor (PB8), 50% feed of betamelor (PB5), and 20% feed of betamelor (PB2). Betamelor intervention was given as much as 5% of weight for 28 days. Fasting Blood Glucose (FBG) levels were measured using the GOD-PAP method. Blood glucose data were analyzed by Analysis of Variance (ANOVA) at a 95% confidence level and using Duncan's test.

Results: There were differences in FBG between groups after the intervention of betamelor. The results showed that after 28 days of intervention, betamelor decreased the serum glucose concentration from 122.69 mg / dL to 97.70 mg / dL (20.37%) in the PB8 group and from 123.91 mg / dL to 113.28 mg / dL (8.58%) in the PB5 group, but the standard diet (PS) increased by 5.73%. This result can be applied to reduce blood glucose levels in obese and patients with metabolic syndrome.

Conclusions: There was a significant effect of giving a mixture of black rice, red beans, and Moringa leaves on fasting blood sugar in rats.

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Keywords: Black rice; Red beans; Moringa leaves; Fasting blood glucose

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  1. Retnaningsih C, Darmono, Widianarko B, Muis SF. Peningkatan aktivitas antioksidan superoksida diamutase pada tikus hiperglikemi dengan asupan tempe koro benguk (Mucuna pruriens L).Agritech. 2013: 33(2):154–161
  2. Ighodaro OM, Akinloye OA. First line defence antioxidants-superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX): Their fundamental role in the entire antioxidant defence grid. Alexandria Med J. 2017;54(4):287–293
  3. Mansuroglu B, Derman S, Yaba A, Kizilbey K. Protective effect of chemically modified SOD on lipid peroxidation and antioxidant status in diabetic rats. Int J Biol Macromol. 2014;72:79–87
  4. Subramoniam, A. Anti-Diabetes Mellitus Plants. Boca Raton: CRC Press; 2016
  5. Walter M, Marchesan E.Phenolic Compounds and Antioxidant Activity of Rice. Brazilian A Bio Tech. 2011; Vol. 54 (2): 371–377
  6. Goufo P, Trindade H. Rice Antioxidants: Phenolic Acids, Flavonoids, Anthocyanins, Proanthocyanidins, Tocopherols, Tocotrienols, γ-Oryzanol, and Phytic Acid. F Sci Nutr. 2013; Vol. 2 (2): 75–104
  7. Wanti S, Andriani M.A.M, Parnanto N.H.R. Pengaruh Berbagai Jenis Beras terhadap Aktivitas Antioksidan pada Angkak oleh Monascus purpureus. Biofarmasi. 2015;Vol. 13 (1): 1–5
  8. Audu SS, Aremu MO. Effect of process- ing on chemical composition of red kidney bean (Phaseolus vulgalris L.) flour. PJN. 2011;10(11):1069-1075
  9. Messina. Nutrition and health benefit of dried bean. Am J Clin Nutr. 2014; 100 (suppl): 437S-442S
  10. Fidriannyi I, Nuraini P, Marlia SW. Antioxidant activities, total flavonoid, phenolic, carotenoid of various shells extracts from four species of legumes. Asian J Pharm Clin Res. 2014; 7(4):42-46
  11. Dieman VV, Trindale EN, Trindale MRM. Experimental model to induce obesity in rats. Acta Cir Bras. 2006;21(6):425-429
  12. Izadi, V., Saraf-Bank, S., & Azadbakht, L. Dietary intakes and leptin concentrations. ARYA atherosclerosis.2014;10(5), 266–272
  13. Crescenzo R, Bianco F, Coppola P, Mazzoli A, Tussellino M, Carotenuto R, et al. Fructose supplementation worsens the deleterious effects of short-term high-fat feeding on hepatic steatosis and lipid metabolism in adult rats. Exp Physiol. 2014;99(9):1203-13
  14. Mbikay, Majambu. Therapeutic Potential of Moringa oleifera Leaves in Chronic Hyperglycemia and Dyslipidemia: A Review. Front Pharmacol. 2012; 3:24
  15. Al-Malki, A.L, dan El Rabey, H.A. The Antidiabetic Effect of Low Doses of Moringa oleifera lam. Seeds on Streptozotocin Induced Diabetes and Diabetic Nephropathy in Male Rats. Biomed research International. 2015: 1-13
  16. Tantipaiboonwonga P, Pinthaa K, Chaiwangyena W, Chewonarinb T, Pangjitc K, Chumphukama O,et.al. Anti hyperglycaemic and Anti hyperlipidaemic Effects of Black and Red Rice in Streptozotocin Induced Diabetic Rats. ScienceAsia. 2017;Vol. 43: 281– 288
  17. Chaiyasut C, Sivamaruthi B.S, Pengkumsri N, Keapai W, Kesika P, Saelee M, Tojing P, et.al. Germinated Thai Black Rice Extract Protects Experimental Diabetic Rats from Oxidative Stress and Other Diabetes-Related Consequences. Pharmaceuticals. 2017; Vol. 10 (3): 2–16
  18. Chung S.I, Kang M.Y, Lee S.C. Inhibitory Effect of Germinated Pigmented Rice on Key Metabolic Enzymes Associated with Diabetes and Hyperglycemia. In JF Nutr Scie. 2016; Vol. 5 (4): 32–40
  19. Kasim E, Astuti S, Nurhidayat N. Karakterisasi Pigmen dan Kadar Lovastatin Beberapa Isolat Monascus purpureus. Biodiversitas. 2005;6 (4): 245–247
  20. Hosoda K, Sasahara H, Matsushita K, Tamura Y, Miyaji M, Matsuyama H. Anthocyanin and proanthocyanidin contents, antioxidant activity, and in situ degradability of black and red rice grains. Asian-Australas J An Scie. 2018; Vol. 31 (8): 1213–1220
  21. Rimbawan.Indeks glikemik pangan: cara mudah memilih pangan yang menyehatkan. Jakarta: Penebar Swadaya;2004
  22. Audu SS, and MO Aremu. Effect of Processing on Chemical Composition of Red Kidney Bean (Phaseolus vulgaris L .) Flour. Pakistan J Nutr. 2011;10 (11):1069–75
  23. Monti LD, MCC asiraghi, E Setola, E Galluccio, MA Pagani, L Quaglia, et al,. L-Arginin Enriched Biscuit Improve Endothelial Function and Glucose Metabolism: A Pilot Study in Healthy Subjects and A Cross-over Study in Subjects with Impaired Glucose Tolerance and Metabolic Syndrome. Met Clin Ex. 2013;62: 255-264
  24. Yao Y, Cheng X, Ren G. Alpha-Glucosidase inhibitory activity of protein-rich extracts from extruded adzuki bean in diabetic KK-Ay mice. Food Func. 2014;5(5):966–971
  25. Liu, Y., Wang, Q., Li, S., Yue, Y., Ma, Y., & Ren, G. Convenient food made of extruded adzuki bean attenuates inflammation and improves glycemic control in patients with type 2 diabetes: a randomized controlled trial. Ther clin risk man.2018;14, 871–884
  26. Yao Y, Cheng X, Wang L, Wang S, Ren G. A determination of potential alpha-glucosidase inhibitors from Azuki Beans (Vigna angularis) Int J Molecular Sci. 2011;12(10):6445–6451

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