DOI: https://doi.org/10.14710/jkli.21.1.43-49

Identifikasi Escherichia coli Penghasil ESBL dari Efluen Rumah Potong Hewan Unggas di Kota Bogor, Jawa Barat

1Sekolah Pasca Sarjana Program Studi Kesehatan Masyarakat Veteriner, Fakultas Kedokteran Hewan, Institut Pertanian Bogor, Jawa Barat 16680, Indonesia

2Balai Pengujian Mutu dan Sertifikasi Produk Hewan, Jl. Pemuda No.29A Bogor 16610, Indonesia

3Divisi Kesehatan Masyarakat Veteriner dan Epidemiologi dan Kesehatan Masyarakat Veteriner, Fakultas Kedokteran Hewan, Institut Pertanian Bogor, Jawa Barat 16680, Indonesia

Open Access Copyright 2022 Jurnal Kesehatan Lingkungan Indonesia under http://creativecommons.org/licenses/by-sa/4.0.

Citation Format:
Abstract

Latar belakang: Escherichia coli merupakan bakteri enterik komensal yang mudah resistan terhadap antibiotik karena memiliki kemampuan bawaan dalam menghasilkam enzim resistansi antibiotik, salah satunya Extended Spectrum Beta Lactamase (ESBL). Bakteri ini dapat ditularkan melalui lingkungan, salah satunya melalui cemaran efluen dari rumah potong hewan unggas (RPH-U) dan Tempat Pemotongan Ayam (TPA). Tujuan penelitian ini adalah untuk mengisolasi, mengidentifikasi, dan mengukur jumlah bakteri E. coli penghasil ESBL dari RPH-U/TPA di Kota Bogor.

Metode: Disain penelitian yang digunakan adalah penelitian eksploratif dengan metode pengambilan sampel secara purposive sampling. Sampel yang diambil berjumlah 12 dari tiga RPH-U/TPA yang memiliki kapasitas pemotongan lebih dari 1000 ekor/hari. Metode yang digunakan adalah ESBL Ec Tricycle dan dilakukan di laboratorium Balai Pengujian Mutu dan Sertifikasi Produk Hewan. Data dibahas secara deskriptif dan disajikan berupa gambar dan tabel. 

Hasil: Escherichia coli penghasil ESBL teridentifikasi dari semua 12 sampel yang berasal dari tiga RPH-U/TPA di Kota Bogor dengan persentase E.coli penghasil ESBL terendah 10,45% dan terbesar yaitu 39,52% dengan rata-rata 17,76%.

Simpulan: Escherichia coli penghasil ESBL ditemukan pada efluen dari ketiga RPH-U/TPA di Kota Bogor.  Bakteri tersebut berisiko mencemari lingkungan melalui efluen dan dapat menjadi ancaman kesehatan terutama terhadap masyarakat yang berada di sepanjang aliran sungai. Langkah mitigasi dan pencegahan perlu dilakukan.

 

Title: Identification of  ESBL Producing Escherichia coli  from Poultry Abattoir Effluent in Bogor, West Java

Background: Escherichia coli is a commensal enteric bacteria that is easily resistant to antibiotics because it has an innate ability to produce antibiotic resistance enzymes, one of which is Extended Spectrum Beta Lactamase (ESBL). These bacteria could been transmitted through the environment from poultry abattoir (RPH-U) and chicken slaughterhouses (TPA) effluent contamination. The purpose of this study was to isolate, identify, and enumerate the number of ESBL-producing E. coli bacteria from RPH-U/TPA in Bogor City

Method:  The research design used is exploratory research with purposive sampling method. 12 samples were taken from 3 RPH-U/TPA which have a slaughtering capacity of more than 1000 head/day. The method used is the ESBL Ec Tricycle and is carried out in the Center for Quality Testing and Certification of Animal Products laboratory. Data discussed descriptively and presented in the form of pictures and tables.

Result: Escherichia coli producing ESBL was identified from all 12 samples from 3 RPH-U/TPA in Bogor City with the smallest ESBL-producing E.coli percentage of 10.45% and the largest 39.52% with an average of 17.76%.

Conclusion: Escherichia coli producing ESBL was found in the effluent of the three RPH-U/TPA in Bogor City. These bacteria are at risk of contaminating the environment through effluents and can pose a health threat, especially to people living along the river. Mitigation and prevention measures need to be taken.

 

Note: This article has supplementary file(s).

Fulltext View|Download |  ES
Ethical Statement
Subject
Type ES
  Download (450KB)    Indexing metadata
 CTA
Copyright Transfer Agreement
Subject
Type CTA
  Download (439KB)    Indexing metadata
 Turnitin
Turnitin
Subject
Type Turnitin
  Download (2MB)    Indexing metadata
Keywords: Escherichia coli; Resistansi antibiotik; RPH-U
Funding: BPPSDMP Kementerian Pertanian

Article Metrics:

Article Info
Section: Research Articles
Language : ID
Recent articles
Identifikasi Escherichia coli Penghasil ESBL dari Efluen Rumah Potong Hewan Unggas di Kota Bogor, Jawa Barat Analisis Risiko Kesehatan Akibat Pajanan Senyawa Nitrat dan Nitrit Pada Air Tanah di Desa Cihambulu Subang Penurunan Konsentrasi COD Limbah Batik Pada Proses Seeding dan Aklimatisasi Menggunakan Material Preservasi Mikroorganisme (MPMO) Halaman Belakang Halaman Sampul More recent articles
Most cited articles
Faktor Risiko Lingkungan Fisik Rumah dan Karakteristik Wilayah Sebagai Determinan Kejadian Penyakit Tuberkulosis Paru di Wilayah Kerja Puskesmas Sentani Kabupaten Jayapura Provinsi Papua Peningkatan Pengetahuan dan Sikap Kepala Keluarga tentang Pengelolaan Sampah Melalui Pemberdayaan Keluarga di Kelurahan Tamansari Kota Bandung Faktor Risiko dan Potensi Penularan Tuberkulosis Paru di Kabupaten Kendal , Jawa Tengah Faktor-Faktor Yang Berhubungan Dengan Angka Kuman Dalam Air Produk Air Minum Isi Ulang di Pemalang Faktor Risiko Kebiasaan Tinggal di Rumah Etnis dan Membuang Dahak Sembarang pada Kejadian TB Paru Di Kabupaten Jayawijaya, Papua More cited articles
  1. Niasono AB, Latif H, Purnawarman T. Resistensi antibiotik terhadap bakteri Escherichia coli yang diisolasi dari peternakan ayam pedaging di Kabupaten Subang, Jawa Barat. Jurnal Veteriner 2019,20(2):187–95
  2. O’Neill J. Review on antibiotic resisitance. Antimicrobial resistance : tackling a crisis for the health and wealth of nations [Internet]. 2014 [dikutip 1 Oktober 2020 Oct 1]. Tersedia pada : https://wellcomecollection.org/works/ rdpck35v/items
  3. WHO. WHO publishes list of bacteria for which new antibiotics are urgently needed [Internet]. 2017 [dikutip 31 Januari 2020]. Tersedia pada: https://www.who.int/news/ item/27-02-2017-who-publishes-list-of-bacte ria-for-which-new-antibiotics-are-urgently-needed
  4. Zhang X, Xiao S, Jiang X, Li Y, Fan Z, Yu Y, et al. Genomic characterization of Escherichia coli LCT-EC001, an extremely multidrug-resistant strain with an amazing number of resistance genes. Gut Pathogens 2019,11(1):1–8. https://doi.org/10.1186/s13099-019-0298-5
  5. Varga C, Rajić A, McFall ME, Reid-Smith RJ, Deckert AE, Pearl DL, et al. Comparison of antimicrobial resistance in generic Escherichia coli and Salmonella spp. cultured from identical fecal samples in finishing swine. Canadian Journal of Veterinary Research 2008,72(2 SPEC. ISS.):181–187
  6. Uemura M, Imataki O, Uchida S, Nakayama-Imaohji H, Ohue Y, Matsuka H, et al. Strain specific transmission in an outbreak of ESBL producing Enterobacteriaceae in the hemato-oncology care unit: a cohort study. BMC Infectious Diseases 2017,17(1):1–8. https://doi.org/10.1186/s12879-016-2144-4
  7. ISO. ISO 19458 : 2006 Water quality – Sampling for microbiological. Geneva (CH): International Organization for Standardization; 2006
  8. BSN. SNI 6989.57-2008 Pengambilan contoh air permukaan. Jakarta: Badan Standardisasi Nasional; 2008
  9. CLSI. CLSI M100-ED31:2021 Performance Standards for Antimicrobial Susceptibility Testing, 31st Edition [Internet]. 2021 [dikutip 31 Januari 2021]. Tersedia pada: http://em100.edaptivedocs.net/Login.aspxr
  10. WHO. WHO integrated global surveillance on ESBL-producing E. coli using a “One Health” approach [Internet]. 2021 [dikutip 31 Januari 2021]. Tersedia pada:: https://www.who.int/ publications/i/item/who-integrated-global-surveillance-on-esbl-producing-e.-coli-using-a-one-health-approach
  11. Jozić S, Vukić Lušić D, Aljinović A, Vlakančić W, Cenov A, Vrdoljak Tomaš A, et al. Is TBX agar a suitable medium for monitoring Escherichia coli in bathing water using the membrane filtration method? Environmental Monitoring and Assessment 2019,191(9):558–69. https://doi.org/10.1007/s10661-019-7733-4
  12. Wanger A. Microbiology and Molecular Diagnosis in Pathology Chapter 4 : Media for the Clinical Microbiology Laboratory. In: Microbiology and Molecular Diagnosis in Pathology London (UK): Elsevier Ltd; 2017. https://doi.org/10.1016/B978-0-12-805351-5.00004-1
  13. Rahayu KP, Andriani MD, Wahyudi A. Identifikasi Escherichia coli penghasil Extendedspectrum Β-Lactamase (ESBL) pada sampel limbah air dari proses pemotongan di tempat pemotongan unggas Kota Bogor. Prosiding Penyakit Hewan Rapat Teknis dan Pertemuan Ilmiah (Ratekpil) dan Surveilans Kesehatan Hewan 2020,10(1):367–74
  14. Febrianti T, Nursofiah S, Amalia N, Febriyana D. Performa tryptone bile x-glucuronide (TBX) yang disuplementasikan dengan Cefotaxime sebagai medium selektif untuk skrining ESBL-E .coli dari sampel lingkungan. Buletin Penelitian Kesehatan 2021,49(1):1–8. https://doi.org/10.22435/bpk.v49i1.3858
  15. Blaak H, Lynch G, Italiaander R, Hamidjaja RA, Schets FM, De Husman AMR. Multidrug-resistant and extended spectrum beta-lactamase-producing escherichia coli in dutch surface water and wastewater. PLoS ONE 2015,10(6). https://doi.org/10.1371/journal.pone.0127752
  16. Kalanjati WW. Keberadaan Escherichia coli Penghasil Extended Spectrum B-lactamase Di Lingkungan Sentra Pemotongan Ayam Pondok Rumput Kota Bogor [tesis]. Bogor : Institut Pertanian Bogor; 2017
  17. Sasongko H. Uji Resistensi Bakteri Escherichia Coli dari Sungai Boyong Kabupaten Sleman terhadap Antibiotik Amoksisilin. Jurnal Bioedukatika 2014,2(1):25–9. https://doi.org/10.26555/bioedukatika.v2i1.4108
  18. Soraas A, Sundsfjord A, Sandven I, Brunborg C, Jenum PA. Risk factors for community-acquired urinary tract infections caused by ESBL-producing enterobacteriaceae -a case-control study in a low prevalence country. PLoS ONE 2013,8(7):1–7. https://doi.org/10.1371/journal.pone.0069581
  19. Guenther S, Ewers C, Wieler LH. Extended-spectrum beta-lactamases producing E. coli in wildlife, yet another form of environmental pollution? Frontiers in Microbiology 2011,2:246. https://doi.org/10.3389/fmicb.2011.00246
  20. Franz E, Veenman C, van Hoek AHA., Husman A de R, Blaak H. Pathogenic Escherichia coli producing Extended-Spectrum β-Lactamases isolated from surface water and wastewater. Scientific Reports 2015,5(14372). https://doi.org/10.1038/srep14372
  21. Wadepohl K, Müller A, Seinige D, Rohn K, Blaha T, Meemken D, et al. Association of intestinal colonization of ESBL producing Enterobacteriaceae in poultry slaughterhouse workers with occupational exposure-a german pilot study. PLoS ONE 2020,15(6):1–12. https://doi.org/10.1371/journal.pone.0232326
  22. Kardaś-Słoma L, Yazdanpanah Y, Perozziello A, Zahar J-R, Lescure F-X, Cousien A, et al. Hand hygiene improvement or antibiotic restriction to control the household transmission of extended-spectrum β-lactamase-producing Escherichia coli: a mathematical modelling study. Antimicrobial Resistance & Infection Control [Internet] 2020,9(1):139–47. Available from: https://doi.org/10.1186/s13756-020-00803-9
  23. Humaida R. Strategy to handle resistance of antibiotics. Jurnal Majority 2014,3(7):113–20

Last update:

No citation recorded.

Last update: 2024-12-26 09:16:28

No citation recorded.