Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University, Indonesia
BibTex Citation Data :
@article{JKSA15213, author = {Annisa Rachma and Purbowatiningrum Sarjono and Agustina Aminin}, title = {Isolasi Bakteri Termofilik Sumber Air Panas Gedongsongo dengan Media Pengaya Minimal YT (Yeast Tripton) serta Identifikasi Genotipik dan Fenotipik}, journal = {Jurnal Kimia Sains dan Aplikasi}, volume = {12}, number = {3}, year = {2009}, keywords = {termofilik; isolasi DNA; enzim ekstraseluler}, abstract = {Eksplorasi mikroorganisme baru merupakan suatu hal yang penting, karena mikroorganisme mempunyai potensi yang sangat luas sebagai sumber biomolekul baru seperti enzim, antimikroba, antikanker, agen bioremediasi, dan penghasil bahan bakar hayati. Hingga saat ini, kurang dari 1% bakteri di alam yang berhasil dikultivasi. Kebutuhan industri terhadap biomolekul yang tahan terhadap kondisi ekstrem sangatlah tinggi. Salah satu sumber biomolekul yang potensial adalah bakteri termofilik. Indonesia kaya akan habitat termofilik, khususnya sumber air panas, namun eksplorasinya masih sangat terbatas. Penelitian ini bertujuan untuk mendapatkan isolat tunggal bakteri termofilik dari sumber air panas Gedong Songo dengan media minimal YT (Yeast Tripton) dalam kondisi anaerob serta melakukan identifikasi bakteri termofilik tersebut secara genotipik dan fenotipik. Pada penelitian ini dilakukan isolasi bakteri termofilik sumber air panas Gedongsongo dengan media pengaya minimal YT pada kondisi anaerob serta identifikasi bakteri secara genotipik dan fenotipik. Identifikasi genotipik dilakukan dengan menggunakan analisis urutan nukleotida gen 16S rRNA. Identifikasi fenotipik dilakukan melalui uji morfologi meliputi uji pewarnaan gram dan uji mikroskop. Selain itu dilakukan uji potensi enzim ekstraseluler termostabil, meliputi amilase, protease, β-galaktosidase, dan selulase. Hasil penelitian diperoleh isolat tunggal bakteri termofilik anaerob yang diberi nama isolat GS Ytan, dengan karakteristik bentuk batang, gram positif, dan memiliki kemiripan dengan bakteri kelompok Anoxybacillus sp. dengan tingkat kekerabatan sebesar 97-99 %. Isolat GS YTan memiliki hubungan terdekat dengan Anoxybacillus sp. AF/04 dan Anoxybacillus sp. HT14. Isolat GS YTan memiliki potensi enzim ekstraseluler termostabil amilase, protease dan β-galaktosidase.}, issn = {2597-9914}, pages = {66--71} doi = {10.14710/jksa.12.3.66-71}, url = {https://ejournal.undip.ac.id/index.php/ksa/article/view/15213} }
Refworks Citation Data :
Article Metrics:
Last update:
Last update: 2024-11-24 08:15:55
Short communication: The emergence and rise of indigenous thermophilic bacteria exploration from hot springs in Indonesia
As an article writer, the author has the right to use their articles for various purposes, including use by institutions that employ authors or institutions that provide funding for research. Author rights are granted without special permission.
Author who publishes a paper at JKSA has the broad right to use their work for teaching and scientific purposes without the need to ask permission, including: used for (i) teaching in the author's class or institution, (ii) presentation at meetings or conferences and distributing copies to participants ; (iii) training conducted by the author or author's institution; (iv) distribution to colleagues for research use; (v) use in the compilation of subsequent authors' works; (vi) inclusion in a thesis or dissertation; (vi) reuse of part of the article in another work (with citation); (vii) preparation of derivative works (with citation); (viii) voluntary posting on open websites operated by authors or author institutions for scientific purposes (follow the CC BY-SA License).
Authors and readers can copy and redistribute material in any media or format, and mix, modify, and build material for any purpose but they must provide appropriate credit (provide article citation or content), providing links to the license, and indicate if there are changes.
The authors submitting a manuscript do so on the understanding that if accepted for publication, copyright of the article shall be assigned to Jurnal Kimia Sains dan Aplikasi (JKSA). Copyright encompasses rights to reproduce and deliver the article in all form and media, including reprints, photographs, microfilms and any other similar reproductions, as well as translations.
Reproduce any part of this journal, its storage in the database or its transmission by all forms or media is permitted does not need for written permission from JKSA. However, it should be cited as an honor in academic manners
JKSA and the Chemistry Department of Diponegoro University and the Editor make every effort to ensure that there are no data, opinions, or false or misleading statements published in JKSA. However, the content of the article is the sole and exclusive responsibility of each author.
The Copyright Transfer Form can be downloaded here: [Copyright Transfer Form - Indonesian] [Copyright Transfer Form - English]. The copyright form should be signed originally and send to the Editor in the form of printed letters, scanned documents sent via email or fax.
Adi Darmawan, Ph.D (Editor in Chief)
Editor in chief of Jurnal Kimia Sains dan Aplikasi (JKSA)
Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University
Visitor: View My Stats
Jurnal Kimia Sains dan Aplikasi is indexed in:
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
