1Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University, Indonesia
2Otolaryngology Department, Faculty of Medicine, Diponegoro University, Indonesia
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@article{JKSA17424, author = {Citrawati Suprobo and Suprihati Suprihati and Wuryanti Wuryanti}, title = {Uji Antikanker Isolat Bioaktif L-Asparaginase dari Kunyit Putih (Curcuma mangga Val.) terhadap Sel Kanker Serviks}, journal = {Jurnal Kimia Sains dan Aplikasi}, volume = {14}, number = {2}, year = {2011}, keywords = {L-asparaginase; Curcuma mangga Val.; antikanker; sel Hela}, abstract = {Kunyit putih ( Curcuma mangga Val.) merupakan salah satu sumber L- asparaginase yang berpotensi sebagai bioaktif antikanker. Tujuan penelitian ini adalah untuk menentukan nilai LC 50 dan potensi antikanker enzim L- asparaginase dari kunyit putih pada kultur sel kanker serviks (Hela). Enzim L- asparaginase diisolasi dengan metode ekstraksi kemudian dimurnikan dengan fraksinasi dan dialisis. Uji aktivitas enzim L- asparaginase dilakukan dengan metode Nessler sedangkan uji kadar protein dengan metode Lowry. Aktivitas spesifik ditentukan melalui perbandingan unit aktivitas terhadap kadar protein. Fraksi enzim L- asparaginase yang memiliki aktivitas spesifik tertinggi diuji sitotoksisitasnya terhadap sel Hela dengan metode MTT (3-(4,5-dimetiltiazol-2-il)-2,5-difeniltetrazoliumbromida) dan potensi antikankernya ditentukan dengan menghitung nilai LC 50 . Hasil penelitian menunjukkan bahwa enzim L- asparaginase dapat diisolasi dan dimurnikan dari kunyit putih dengan berat enzim fraksi 5 yaitu 0,0097 g dari 1000 g bahan segar. Aktivitas spesifik tertinggi terdapat pada fraksi 5 sebesar 247,925 unit/mg protein. Uji sitotoksisitas pada sel Hela menunjukkan nilai LC 50 sebesar 122,462 μg/mL. Dapat disimpulkan bahwa enzim L- asparaginase fraksi 5 dari kunyit putih mampu menghambat pertumbuhan sel Hela, namun kurang potensial sebagai agen antikanker berdasarkan standar NCI (National Cancer Institute).}, issn = {2597-9914}, pages = {58--63} doi = {10.14710/jksa.14.2.58-63}, url = {https://ejournal.undip.ac.id/index.php/ksa/article/view/17424} }
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