Uji Antikanker Isolat Bioaktif L-Asparaginase dari Kunyit Putih (Curcuma mangga Val.) terhadap Sel Kanker Serviks

Citrawati Octavia Suprobo; Suprihati Suprihati; Wuryanti Wuryanti

doi:10.14710/jksa.14.2.58-63

DOI: https://doi.org/10.14710/jksa.14.2.58-63

Uji Antikanker Isolat Bioaktif L-Asparaginase dari Kunyit Putih (Curcuma mangga Val.) terhadap Sel Kanker Serviks

Citrawati Octavia Suprobo¹, Suprihati Suprihati², Wuryanti Wuryanti¹

¹Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University, Indonesia

²Otolaryngology Department, Faculty of Medicine, Diponegoro University, Indonesia

Published: 1 Aug 2011.

BibTex Citation Data :

@article{JKSA17424,
    author = {Citrawati Suprobo and Suprihati Suprihati and Wuryanti Wuryanti},
    title = {Uji Antikanker Isolat Bioaktif L-Asparaginase dari Kunyit Putih (Curcuma mangga Val.) terhadap Sel Kanker Serviks},
    journal = {Jurnal Kimia Sains dan Aplikasi},
  volume = {14},
    number = {2},
    year = {2011},
    keywords = {L-asparaginase; Curcuma mangga Val.; antikanker; sel Hela},
    abstract = {Kunyit putih ( Curcuma mangga  Val.) merupakan salah satu sumber L- asparaginase  yang berpotensi sebagai bioaktif antikanker. Tujuan penelitian ini adalah untuk menentukan nilai LC 50  dan potensi antikanker enzim L- asparaginase  dari kunyit putih pada kultur sel kanker serviks (Hela). Enzim L- asparaginase  diisolasi dengan metode ekstraksi kemudian dimurnikan dengan fraksinasi dan dialisis. Uji aktivitas enzim L- asparaginase  dilakukan dengan metode Nessler sedangkan uji kadar protein dengan metode Lowry. Aktivitas spesifik ditentukan melalui perbandingan unit aktivitas terhadap kadar protein. Fraksi enzim L- asparaginase  yang memiliki aktivitas spesifik tertinggi diuji sitotoksisitasnya terhadap sel Hela dengan metode MTT (3-(4,5-dimetiltiazol-2-il)-2,5-difeniltetrazoliumbromida) dan potensi antikankernya ditentukan dengan menghitung nilai LC 50 . Hasil penelitian menunjukkan bahwa enzim L- asparaginase  dapat diisolasi dan dimurnikan dari kunyit putih dengan berat enzim fraksi 5 yaitu 0,0097 g dari 1000 g bahan segar. Aktivitas spesifik tertinggi terdapat pada fraksi 5 sebesar 247,925 unit/mg protein. Uji sitotoksisitas pada sel Hela menunjukkan nilai LC 50  sebesar 122,462 μg/mL. Dapat disimpulkan bahwa enzim L- asparaginase  fraksi 5 dari kunyit putih mampu menghambat pertumbuhan sel Hela, namun kurang potensial sebagai agen antikanker berdasarkan standar NCI (National Cancer Institute).},
   issn = {2597-9914},   pages = {58--63}  doi = {10.14710/jksa.14.2.58-63},
    url = {https://ejournal.undip.ac.id/index.php/ksa/article/view/17424}
}

Citation Format:

Abstract

Kunyit putih (Curcuma mangga Val.) merupakan salah satu sumber L-asparaginase yang berpotensi sebagai bioaktif antikanker. Tujuan penelitian ini adalah untuk menentukan nilai LC₅₀ dan potensi antikanker enzim L-asparaginase dari kunyit putih pada kultur sel kanker serviks (Hela). Enzim L-asparaginase diisolasi dengan metode ekstraksi kemudian dimurnikan dengan fraksinasi dan dialisis. Uji aktivitas enzim L-asparaginase dilakukan dengan metode Nessler sedangkan uji kadar protein dengan metode Lowry. Aktivitas spesifik ditentukan melalui perbandingan unit aktivitas terhadap kadar protein. Fraksi enzim L-asparaginase yang memiliki aktivitas spesifik tertinggi diuji sitotoksisitasnya terhadap sel Hela dengan metode MTT (3-(4,5-dimetiltiazol-2-il)-2,5-difeniltetrazoliumbromida) dan potensi antikankernya ditentukan dengan menghitung nilai LC₅₀. Hasil penelitian menunjukkan bahwa enzim L-asparaginase dapat diisolasi dan dimurnikan dari kunyit putih dengan berat enzim fraksi 5 yaitu 0,0097 g dari 1000 g bahan segar. Aktivitas spesifik tertinggi terdapat pada fraksi 5 sebesar 247,925 unit/mg protein. Uji sitotoksisitas pada sel Hela menunjukkan nilai LC₅₀ sebesar 122,462 μg/mL. Dapat disimpulkan bahwa enzim L-asparaginase fraksi 5 dari kunyit putih mampu menghambat pertumbuhan sel Hela, namun kurang potensial sebagai agen antikanker berdasarkan standar NCI (National Cancer Institute).

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Keywords: L-asparaginase; Curcuma mangga Val.; antikanker; sel Hela

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In Vol 14, No 2 (2011): Volume 14 Issue 2 Year 2011

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