Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University, Indonesia
BibTex Citation Data :
@article{JKSA18390, author = {Yanidya Ardiansyah and Nies Mulyani and Purbowatiningrum Sarjono}, title = {Isolasi dan Karakterisasi Enzim Xilanase dari Bacillus Subtilis pada Media Nutrient Broth dengan Penambahan Xilan Hasil Isolasi Jerami Padi}, journal = {Jurnal Kimia Sains dan Aplikasi}, volume = {17}, number = {3}, year = {2014}, keywords = {Jerami padi; Bacillus subtilis; Xilan; Xilanase}, abstract = {Jerami padi merupakan limbah lignoselulosa yang mengandung xilan. Xilan pada jerami padi dapat dimanfaatkan sebagai sumber karbon, substrat dan inducer pada media pertumbuhan Bacillus subtilis untuk menghasilkan xilanase yang memiliki kemampuan menghidrolisis xilan menjadi xilosa atau xilooligosakarida. Pada penelitian ini dilakukan beberapa tahap, yaitu isolasi xilan dari jerami padi, penentuan kurva pertumbuhan Bacillus subtilis, produksi xilanase, pemurnian xilanase dengan fraksinasi bertingkat amonium sulfat dan dialisis. Pengujian aktivitas xilanase dilakukan dengan menggunakan metode DNS, sedangkan kadar protein diuji dengan menggunakan metode Lowry. Aktivitas spesifik xilanase dihitung berdasarkan unit aktivitas xilanase per mg protein. Selanjutnya dilakukan karakterisasi pada fraksi dengan aktivitas spesifik tertinggi meliputi suhu, pH dan waktu inkubasi. Hasil menunjukkan bahwa aktivitas spesifik tertinggi diperoleh pada fraksi F2 yaitu sebesar 5,178 Unit/mg protein. Kondisi optimum xilanase yang diperoleh dari hasil karakterisasi yaitu pada suhu 40°C, pH 8, waktu inkubasi 30 menit dan aktivitas spesifik fraksi F2 pada kondisi optimum sebesar 6,088 Unit/mg protein.}, issn = {2597-9914}, pages = {95--99} doi = {10.14710/jksa.17.3.95-99}, url = {https://ejournal.undip.ac.id/index.php/ksa/article/view/18390} }
Refworks Citation Data :
Article Metrics:
Last update:
The study of xylanase immobilize enzyme using sol-gel method
Last update: 2024-12-26 23:34:22
As an article writer, the author has the right to use their articles for various purposes, including use by institutions that employ authors or institutions that provide funding for research. Author rights are granted without special permission.
Author who publishes a paper at JKSA has the broad right to use their work for teaching and scientific purposes without the need to ask permission, including: used for (i) teaching in the author's class or institution, (ii) presentation at meetings or conferences and distributing copies to participants ; (iii) training conducted by the author or author's institution; (iv) distribution to colleagues for research use; (v) use in the compilation of subsequent authors' works; (vi) inclusion in a thesis or dissertation; (vi) reuse of part of the article in another work (with citation); (vii) preparation of derivative works (with citation); (viii) voluntary posting on open websites operated by authors or author institutions for scientific purposes (follow the CC BY-SA License).
Authors and readers can copy and redistribute material in any media or format, and mix, modify, and build material for any purpose but they must provide appropriate credit (provide article citation or content), providing links to the license, and indicate if there are changes.
The authors submitting a manuscript do so on the understanding that if accepted for publication, copyright of the article shall be assigned to Jurnal Kimia Sains dan Aplikasi (JKSA). Copyright encompasses rights to reproduce and deliver the article in all form and media, including reprints, photographs, microfilms and any other similar reproductions, as well as translations.
Reproduce any part of this journal, its storage in the database or its transmission by all forms or media is permitted does not need for written permission from JKSA. However, it should be cited as an honor in academic manners
JKSA and the Chemistry Department of Diponegoro University and the Editor make every effort to ensure that there are no data, opinions, or false or misleading statements published in JKSA. However, the content of the article is the sole and exclusive responsibility of each author.
The Copyright Transfer Form can be downloaded here: [Copyright Transfer Form - Indonesian] [Copyright Transfer Form - English]. The copyright form should be signed originally and send to the Editor in the form of printed letters, scanned documents sent via email or fax.
Adi Darmawan, Ph.D (Editor in Chief)
Editor in chief of Jurnal Kimia Sains dan Aplikasi (JKSA)
Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University
Visitor: View My Stats
Jurnal Kimia Sains dan Aplikasi is indexed in:
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
