Isolation of Phenolic Acid Compounds and Antioxidant Tests from Mindi Leaves (Melia azedarach L.)

DOI: https://doi.org/10.14710/jksa.21.4.198-204

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Article Metrics: (Click on the Metric tab below to see the detail)

Article Info
Published: 31-10-2018
Section: Research Articles
Fulltext PDF Tell your colleagues Email the author
Melia azedarach L plant categorized as traditional medicinal plant is normally used as a medicine for cough, skin diseases, drug for malaria, diabetes, colon cancer, jaundice, vaginal discharge, fever and scabies. Melia azedarach leaves contain steroid, terpenoid, alkaloid, tannin, saponin, phenolic and flavonoid compounds. The purpose of this study was isolation of phenolic acid compounds and antioxidant activity test from Melia azedarach L. Leaves. The method used to isolate phenolic acid using 3 ways that were alkaline hydrolysis (HB), acid hydrolysis (HA), and without hydrolysis (TH). The separation of phenolic acid which has Rf not the same as standard phenolic acid was undertaken by preparative TLC and characterized using UV-Vis spectrophotometer, FT-IR, and LC-MS. Moreover, antioxidant test of phenolic acid using DPPH method was also conducted. The results showed that isolation of Melia azedarach L. Leaves resulted in HB, HA and TH fractions with the weight of 1.05 grams, 1.26 grams and 1.38 grams, respectively. The identification of phenolic acid which has Rf the same as standard phenolic acids was ferulic acid. While result of phenolic acid (FA) isolates which has Rf not the same as phenolic acids standard was caffeic acid after the identifying using UV-Vis spectrophotometer, FTIR and LC-MS. Qualitative antioxidant activity test showed that FA isolate had IC50 of 168,650 ppm. This result indicated that FA isolate is potential as antioxidant compound.

Keywords

Melia azearach L.; phenolic acid; DPPH

  1. Andriyani Budi Listyo 
    Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University Jl. Prof. Soedarto, SH., Tembalang, Semarang, Indonesia
  2. Dewi Kusrini  Scopus Sinta
    Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University Jl. Prof. Soedarto, SH., Tembalang, Semarang, Indonesia
  3. Enny Fachriyah  Scopus Sinta
    Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University Jl. Prof. Soedarto, SH., Tembalang, Semarang, Indonesia
  1. Deepika Sharma, Yash Paul, Preliminary and pharmacological profile of Melia azedarach L.: An overview, Journal of Applied Pharmaceutical Science, 3, 12, (2013) 133-138
  2. Earha Arakkaveettil Kabeer, Remani Prathapan, Phytopharımacological Profile of Elephantopus scaber, Pharmacologia, 5, 8, (2014) 272-285 http://dx.doi.org/10.5567/pharmacologia.2014.272.285
  3. M. M. Azam, A. N. M. Mamun-Or-Rashid, N. M. Towfique, M. K. Sen, Nasrin. S., Pharmacological potentials of Melia azedarach L. - A review, American Journal of BioScience, 1, 2, (2013) 44-49 http://dx.doi.org/10.11648/j.ajbio.20130102.13
  4. Md Asadujjaman, Abu Saed, Md Aslam Hossain, Utpal Kumar Karmakar, Assessment of bioactivities of ethanolic extract of Melia azedarach (Meliaceae) leaves, Journal of Coastal Life Medicine, 1, 2, (2013) 118-122 http://dx.doi.org/10.12980/JCLM.1.2013C548
  5. Garima Mishra, Sunil Jawla, Vikas Srivastava, Melia Azedarach: A Review, International Journal of Medicinal Chemistry & Analysis, 3, 2, (2013) 53-56
  6. Mohammed Fazil Ahmed, A Srinivasa Rao, Shaik Rasheed Ahemad, Mohammed Ibrahim, Phytochemical studies and antioxidant activity of Melia azedarach Linn leaves by DPPH scavenging assay, International Journal of Pharmaceutical Applications, 3, 1, (2012) 271-276
  7. Antara Sen, Amla Batra, Evaluation of antimicrobial activity of different solvent extracts of medicinal plant: Melia azedarach L, International Journal of Current Pharmaceutical Research, 4, 2, (2012) 67-73
  8. Maroua Akacha, Karima Lahbib, Néziha Ghanem Boughanmi, Phytochemically evaluation and net anti-oxidant activity of Tunisian Melia azedarach leaves extract from their ProAntidex parameter, Bangladesh Journal of Pharmacology, 11, 2, (2016) 301-307 http://dx.doi.org/10.3329/bjp.v11i2.25981
  9. Eka Vany Anggraeni, Khairul Anam, Identifikasi Kandungan Kimia dan Uji Aktivitas Antimikroba Kulit Durian (Durio zibethinus Murr.), Jurnal Kimia Sains dan Aplikasi, 19, 3, (2016) 87-93 http://dx.doi.org/10.14710/jksa.19.3.87-93
  10. Jeffrey B. Harborne, Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis, Springer Netherlands, 2012.
  11. R. Zadernowski, M. Naczk, H. Nowak-Polakowska, Phenolic acids of borage (Borago officinalis L.) and evening primrose (Oenothera biennis L.), Journal of the American Oil Chemists' Society, 79, 4, (2002) 335-338 http://dx.doi.org/10.1007/s11746-002-0484-8
  12. Edwin Fadhly, Dewi Kusrini, Enny Fachriyah, Isolasi, Identifikasi Senyawa Alkaloid dari Daun Rivina humilis L. serta Uji Sitotoksik Menggunakan Metode BSLT (Brine Shrimp Lethality Test), Jurnal Kimia Sains dan Aplikasi, 18, 2, (2015) 67-72 http://dx.doi.org/10.14710/jksa.18.2.67-72
  13. Retno Sariningsih, Meiny Suzery, Bambang Cahyono, Uji Aktivitas Antioksidan dengan DPPH fraksi Etil Asetat Daun Bidens pilosa L, Jurnal Kimia Sains dan Aplikasi, 19, 3, (2016) 83-86 http://dx.doi.org/10.14710/jksa.19.3.83-86
  14. Marsden S. Blois, Antioxidant Determinations by the Use of a Stable Free Radical, Nature, 181, (1958) 1199 http://dx.doi.org/10.1038/1811199a0
  15. Rebecca J. Robbins, Phenolic Acids in Foods: An Overview of Analytical Methodology, Journal of Agricultural and Food Chemistry, 51, 10, (2003) 2866-2887 http://dx.doi.org/10.1021/jf026182t
  16. Robert E. Ardrey, Liquid Chromatography - Mass Spectrometry: An Introduction, Wiley, 2003.