Isolation of Phenolic Acid Compounds and Antioxidant Tests from Mindi Leaves (Melia azedarach L.)

Andriyani Budi Listyo; Dewi Kusrini; Enny Fachriyah

doi:10.14710/jksa.21.4.198-204

DOI: https://doi.org/10.14710/jksa.21.4.198-204

Isolation of Phenolic Acid Compounds and Antioxidant Tests from Mindi Leaves (Melia azedarach L.)

Andriyani Budi Listyo, Dewi Kusrini

, Enny Fachriyah

Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University, Indonesia

Received: 6 Aug 2018; Revised: 26 Oct 2018; Accepted: 26 Oct 2018; Published: 31 Oct 2018.

BibTex Citation Data :

@article{JKSA19806,
    author = {Andriyani Listyo and Dewi Kusrini and Enny Fachriyah},
    title = {Isolation of Phenolic Acid Compounds and Antioxidant Tests from Mindi Leaves (Melia azedarach L.)},
    journal = {Jurnal Kimia Sains dan Aplikasi},
  volume = {21},
    number = {4},
    year = {2018},
    keywords = {Melia azearach L.; phenolic acid; DPPH},
    abstract = { Melia azedarach  L plant categorized as traditional medicinal plant is normally used as a medicine for cough, skin diseases, drug for malaria, diabetes, colon cancer, jaundice, vaginal discharge, fever and scabies.  Melia azedarach  leaves contain steroid, terpenoid, alkaloid, tannin, saponin, phenolic and flavonoid compounds. The purpose of this study was isolation of phenolic acid compounds and antioxidant activity test from  Melia azedarach  L. Leaves. The method used to isolate phenolic acid using 3 ways that were alkaline hydrolysis (HB), acid hydrolysis (HA), and without hydrolysis (TH). The separation of phenolic acid which has Rf not the same as standard phenolic acid was undertaken by preparative TLC and characterized using UV-Vis spectrophotometer, FT-IR, and LC-MS. Moreover, antioxidant test of phenolic acid using DPPH method was also conducted. The results showed that isolation of  Melia azedarach  L. Leaves resulted in HB, HA and TH fractions with the weight of 1.05 grams, 1.26 grams and 1.38 grams, respectively. The identification of phenolic acid which has Rf the same as standard phenolic acids was ferulic acid. While result of phenolic acid (FA) isolates which has Rf not the same as phenolic acids standard was caffeic acid after the identifying using UV-Vis spectrophotometer, FTIR and LC-MS. Qualitative antioxidant activity test showed that FA isolate had IC 50  of 168,650 ppm. This result indicated that FA isolate is potential as antioxidant compound.},
   issn = {2597-9914},   pages = {198--204}  doi = {10.14710/jksa.21.4.198-204},
    url = {https://ejournal.undip.ac.id/index.php/ksa/article/view/19806}
}

Citation Format:

Abstract

Melia azedarach L plant categorized as traditional medicinal plant is normally used as a medicine for cough, skin diseases, drug for malaria, diabetes, colon cancer, jaundice, vaginal discharge, fever and scabies. Melia azedarach leaves contain steroid, terpenoid, alkaloid, tannin, saponin, phenolic and flavonoid compounds. The purpose of this study was isolation of phenolic acid compounds and antioxidant activity test from Melia azedarach L. Leaves. The method used to isolate phenolic acid using 3 ways that were alkaline hydrolysis (HB), acid hydrolysis (HA), and without hydrolysis (TH). The separation of phenolic acid which has Rf not the same as standard phenolic acid was undertaken by preparative TLC and characterized using UV-Vis spectrophotometer, FT-IR, and LC-MS. Moreover, antioxidant test of phenolic acid using DPPH method was also conducted. The results showed that isolation of Melia azedarach L. Leaves resulted in HB, HA and TH fractions with the weight of 1.05 grams, 1.26 grams and 1.38 grams, respectively. The identification of phenolic acid which has Rf the same as standard phenolic acids was ferulic acid. While result of phenolic acid (FA) isolates which has Rf not the same as phenolic acids standard was caffeic acid after the identifying using UV-Vis spectrophotometer, FTIR and LC-MS. Qualitative antioxidant activity test showed that FA isolate had IC₅₀ of 168,650 ppm. This result indicated that FA isolate is potential as antioxidant compound.

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Keywords: Melia azearach L.; phenolic acid; DPPH

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Language : ID

In Vol 21, No 4 (2018): volume 21 Issue 4 Year 2018

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