The potential of Endophytic Fungal Extract Isolated from Cinnamon (Cinnamomum burmannii) as Antidiabetic and Antioxidant

*Eris Septiana scopus  -  Biotechnology Research Center, Indonesian Institute of Sciences, Indonesia
Fauzy Rachman scopus  -  Biotechnology Research Center, Indonesian Institute of Sciences, Indonesia
Yatri Hapsari scopus  -  Biotechnology Research Center, Indonesian Institute of Sciences, Indonesia
Yadi Yadi  -  Biotechnology Research Center, Indonesian Institute of Sciences, Indonesia
Bustanussalam Bustanussalam scopus  -  Biotechnology Research Center, Indonesian Institute of Sciences, Indonesia
Siti Irma Rahmawati scopus  -  Biotechnology Research Center, Indonesian Institute of Sciences, Indonesia
Fauzia Nurul izzati orcid scopus  -  Biotechnology Research Center, Indonesian Institute of Sciences, Indonesia
Partomuan Simanjuntak scopus  -  Biotechnology Research Center, Indonesian Institute of Sciences, Indonesia
Received: 13 Aug 2019; Revised: 9 Oct 2019; Accepted: 16 Oct 2019; Published: 30 Nov 2019; Available online: 30 Nov 2019.
Open Access Copyright 2019 Jurnal Kimia Sains dan Aplikasi
License URL: http://creativecommons.org/licenses/by-sa/4.0

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Abstract
An increase in blood glucose levels in people with diabetes can cause an increase in free radicals, which can worsen the disease. Thus, drugs that have antidiabetic and antioxidant activities are needed. The cinnamon plant is high in antioxidants and has long been used as a source for a diabetes drug. The utilization of endophytic fungi isolated from cinnamon plants as antidiabetic and antioxidant has never been reported. This study aims to investigate the antidiabetic as well as antioxidant activity from the extract of endophytic fungi from the cinnamon plant. The antidiabetic activity was tested using the α-glucosidase enzyme inhibition method, while antioxidant activity was tested using the DPPH free radical scavenging method. Total phenol content was measured based on the Follin-Ciocalteu reagent reaction. All endophytic fungal extracts from the cinnamon leaves, twigs, flowers, and fruit have antidiabetic and antioxidant activity as well as high total phenol content. The three parameters measured showed a positive correlation. Endophytic fungal extract of Cb.D6 isolate derived from the leaf had the highest antidiabetic and antioxidant activity among the other isolates amounting to 92.41% and 90.28%, respectively. In addition, the total phenol content of Cb.D6 isolates was also the highest with 357.83 mg equivalent to gallic acid/g extract. Therefore, the endophytic fungal extract of Cb.D6 isolate has the potential to be developed as a source of the antidiabetic and antioxidant ingredients.
Keywords: Endophytic fungus; cinnamon; antidiabetic; antioxidant; total phenol content

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  1. Hardoko, Titri Siratantri, Eveline, Mario Yogabuana and Stella Olivia, An In Vitro Study of Antidiabetic Activity of Sargassum Duplicatum and Turbinaria Decurens Seaweed, International Journal of Pharmaceutical Science Invention, 3, 2, (2014) 13-18
  2. P. Z. Zimmet, Diabetes epidemiology as a tool to trigger diabetes research and care, Diabetologia, 42, 5, (1999) 499-518 https://doi.org/10.1007/s001250051188
  3. Kementrian Kesehatan RI, Riset Kesehatan Dasar 2018, in, Kementrian Kesehatan RI, Jakarta, 2018
  4. Ahmad Nawaz Khan, Rahmat Ali Khan, Mushtaq Ahmad and Nadia Mushtaq, Role of antioxidant in oxidative stress and diabetes mellitus, Journal of pharmacognosy and phytochemistry, 3, 6, (2015) 217-220
  5. Renu A. Kowluru and Pooi-See Chan, Oxidative Stress and Diabetic Retinopathy, Experimental Diabetes Research, 2007, Article ID 43603, (2007) https://doi.org/10.1155/2007/43603
  6. M Ervina, Y.E. Nawu and S.Y. Esar, Comparison of in vitro antioxidant activity of infusion, extract and fractions of Indonesian Cinnamon (Cinnamomum burmannii) bark, International Food Research Journal, 23, 3, (2016) 1346-1350
  7. Anna Priangani Roswiem, Made B Anggriawan and Waras Nurcholis, Potensi Ekstrak Air Dan Etanol Kulit Batang Kayu Manis Padang (Cinnamomum Burmanii) Terhadap Aktivitas Enzim a-Glukosidase, Jurnal Kedokteran YARSI, 23, 2, (2015) 91-102
  8. Amalia Indah Prihantini and Sanro Tachibana, Antioxidant compounds produced by Pseudocercospora sp. ESL 02, an endophytic fungus isolated from Elaeocarpus sylvestris, Asian Pacific Journal of Tropical Biomedicine, 7, 2, (2017) 110-115 https://doi.org/10.1016/j.apjtb.2016.11.020
  9. Anastasia Wheni Indrianingsih and Sanro Tachibana, α-Glucosidase inhibitor produced by an endophytic fungus, Xylariaceae sp. QGS 01 from Quercus gilva Blume, Food Science and Human Wellness, 6, 2, (2017) 88-95 https://doi.org/10.1016/j.fshw.2017.05.001
  10. Salini Dinesh, Devi Soorya Narayana Sasikumar, Bala Girija, Lakshmipriya V Panicker, Pooja Vinod Kumar, Sruthy Preetha and Shaima Santhilal Sarma, Pharmacological evaluation of endophytic Penicillium pimiteouiense SGS isolated from Simarouba glauca DC, Journal of Applied Pharmaceutical Science, 7, 09, (2017) 142-147 https://doi.org/10.7324/JAPS.2017.70919
  11. G. Salini, Anjana Madhusoodhanan, Anju Joseph, Anju Mohan, Navya R. K. and Varsha Venugopal Nair, Antibacterial and antioxidant potential of endophytic fungi isolated from mangroves, Der Pharmacia Lettre, 7, 12, (2015) 53-57
  12. Junichi Saijyo, Yousuke Suzuki, Yoshiharu Okuno, Hidehiko Yamaki, Takuya Suzuki and Mitsuo Miyazawa, α-Glucosidase inhibitor from Bergenia ligulata, Journal of oleo science, 57, 8, (2008) 431-435
  13. Vandana Tiwari, Rakhi Shanker, Jyoti Srivastava and Padma S Vankar, Change in antioxidant activity of spices-turmeric and ginger on heat treatment, Electronic Journal of Environmental, Agricultural and Food Chemistry, 5, (2006) 1313-1317
  14. Anna Pękal and Krystyna Pyrzynska, Evaluation of Aluminium Complexation Reaction for Flavonoid Content Assay, Food Analytical Methods, 7, 9, (2014) 1776-1782 https://doi.org/10.1007/s12161-014-9814-x
  15. Shirly Kumala and Ainun Apriani Pratiwi, Efek antimikroba dari kapang endofit ranting tanaman biduri, Jurnal Farmasi Indonesia, 7, 2, (2015) 111-120
  16. N Saleem Basha, Azieb Ogbaghebriel, Kflom Yemane and Misgana Zenebe, Isolation and screening of endophytic fungi from Eritrean traditional medicinal plant Terminalia brownii leaves for antimicrobial activity, International Journal of Green Pharmacy (IJGP), 6, 1, (2012) 40-44
  17. Fauzy Rachman, Eris Septiana, Sylvia JR Lekatompessy, Tiwit Widowati, Harmastini I Sukiman and Partomuan Simanjuntak, Screening for endophytic fungi from turmeric plant (Curcuma longa L.) of Sukabumi and Cibinong with potency as antioxidant compounds producer, Pakistan Journal of Biological Sciences, 18, 1, (2015) 42-45
  18. Kalyarat Kruawan and Kaew Kangsadalampai, Antioxidant activity, phenolic compound contents and antimutagenic activity of some water extract of herbs, Thai Journal of Pharmaceutical Sciences (TJPS), 30, (2006) 28-35
  19. Menakshi Bhat, Smita S. Zinjarde, Shobha Y. Bhargava, Ameeta Ravi Kumar and Bimba N. Joshi, Antidiabetic Indian Plants: A Good Source of Potent Amylase Inhibitors, Evidence-Based Complementary and Alternative Medicine, 2011, Article ID 810207, (2011) 6 https://doi.org/10.1093/ecam/nen040
  20. Young‐In Kwon, Emmanouil Apostolidis and Kalidas Shetty, Evaluation of pepper (Capsicum annuum) for management of diabetes and hypertension, Journal of Food Biochemistry, 31, 3, (2007) 370-385
  21. Ratna Djamil, Wiwi Winarti, Partomuan Simanjuntak and Syamsudin, Standardization and α-glycosidase inhibition of extracts of Vatica pauciflora Blume stem barks and Smallanthus sonchifolius leaves, Journal of Pharmacognosy and Phytochemistry, 3, 4, (2014) 42-46
  22. N Artanti, S Tachibana, LB Kardono and H Sukiman, Screening of endophytic fungi having ability for antioxidative and alpha-glucosidase inhibitor activities isolated from Taxus sumatrana, Pakistan journal of biological sciences: PJBS, 14, 22, (2011) 1019-1023 https://doi.org/10.3923/pjbs.2011.1019.1023
  23. Madyawati Latief, Fitry Tafzi and Andriyanto Saputra, Aktivitas Antioksidan Ekstrak Metanol Beberapa Bagian Tanaman Kayu Manis (Cinnamomum Burmani) Asal Kabupaten Kerinci Provinsi Jambi, in: Seminar dan Rapat Tahunan (Semirata) Bidang MIPA BKS Barat, Universitas Lampung, Lampung, 2013
  24. Wiwit Denny Fitriana, Taslim Ersam, Kuniyoshi Shimizu and Sri Fatmawati, Antioxidant activity of Moringa oleifera extracts, Indonesian Journal of Chemistry, 16, 3, (2016) 297-301 https://doi.org/10.22146/ijc.21145
  25. G Abirami and M Boominathan, Antioxidant Activity of Endophytic Fungi Isolated from Hugonia mystax L, Journal of Academia and Industrial Research (JAIR), 5, 1, (2016) 10-13
  26. Edward J Dompeipen and P Simanjuntak, Aktivitas antidiabetes dan antioksidan kapang endofit dari tanaman mahoni, Biopropal industri, 6, 1, (2015) 7-17
  27. Stéphanie Dal and Séverine Sigrist, The Protective Effect of Antioxidants Consumption on Diabetes and Vascular Complications, Diseases, 4, 24, (2016) https://doi.org/10.3390/diseases4030024
  28. Ida Y Madiha, Y Rukayadi and H Norhayati, Effects of extraction conditions on yield, total phenolic contents and antibacterial activity of methanolic Cinnamomum zeylanicum Blume leaves extract, International Food Research Journal, 24, 2, (2017) 779-786
  29. Samy Selim, SE Alfy, Meshref Al-Ruwaili, Amin Abdo and SA Jaouni, Susceptibility of imipenem-resistant Pseudomonas aeruginosa to flavonoid glycosides of date palm (Phoenix dactylifera L.) tamar growing in Al Madinah, Saudi Arabia, African Journal of biotechnology, 11, 2, (2012) 416-422 https://doi.org/10.5897/AJB11.1412
  30. Elvira Luján Massó González, Saga Johansson, Mari-Ann Wallander and Luis Alberto García Rodríguez, Trends in the prevalence and incidence of diabetes in the UK: 1996–2005, Journal of Epidemiology & Community Health, 63, 4, (2009) 332-336 https://doi.org/10.1136/jech.2008.080382
  31. Claudio Cabello-Verrugio, Felipe Simon, Capucine Trollet and Juan F Santibañez, Oxidative stress in disease and aging: mechanisms and therapies 2016, Oxidative Medicine and Cellular Longevity, (2016) 4310469 https://doi.org/10.1155/2017/4310469
  32. Bo Meng, Jun Li and Hong Cao, Antioxidant and antiinflammatory activities of curcumin on diabetes mellitus and its complications, Current pharmaceutical design, 19, 11, (2013) 2101-2113 https://doi.org/10.2174/138161213805289318
  33. K. A. Adekola, A. B. Salleh, U. H. Zaidan, A. Azlan, E. Chiavaro, M. Paciulli and J. M. N. Marikkar, Total Phenolic Content, Antioxidative and Antidiabetic Properties of Coconut (Cocos Nucifera L.) Testa and Selected Bean Seed Coats, Italian Journal of Food Science, 29, 4, (2017) 741-753 https://doi.org/10.14674/IJFS-941
  34. Guohua Cao, Emin Sofic and Ronald L. Prior, Antioxidant and Prooxidant Behavior of Flavonoids: Structure-Activity Relationships, Free Radical Biology and Medicine, 22, 5, (1997) 749-760 https://doi.org/10.1016/S0891-5849(96)00351-6
  35. Jacob Vaya, Saeed Mahmood, Amiram Goldblum, Michael Aviram, Nina Volkova, Amin Shaalan, Ramadan Musa and Snait Tamir, Inhibition of LDL oxidation by flavonoids in relation to their structure and calculated enthalpy, Phytochemistry, 62, 1, (2003) 89-99 https://doi.org/10.1016/S0031-9422(02)00445-4

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