Microbial Growth as Determinant of Antibiotic Production with Biotic Elicitors Stimulation

Citra Hardiyanti; Khairullinas Khairullinas; Jeky Sasemar Lumban; Titania Tjandrawati Nugroho; Yuana Nurulita

doi:10.14710/jksa.23.3.89-95

DOI: https://doi.org/10.14710/jksa.23.3.89-95

Microbial Growth as Determinant of Antibiotic Production with Biotic Elicitors Stimulation

Citra Hardiyanti, Khairullinas Khairullinas, Jeky Sasemar Lumban, Titania Tjandrawati Nugroho

, Yuana Nurulita

Chemistry Department, Faculty Mathematics and Natural Sciences, University of Riau, Indonesia

Received: 8 Dec 2019; Revised: 18 Mar 2020; Accepted: 24 Mar 2020; Published: 31 Mar 2020.

BibTex Citation Data :

@article{JKSA27116,
    author = {Citra Hardiyanti and Khairullinas Khairullinas and Jeky Lumban and Titania Nugroho and Yuana Nurulita},
    title = {Microbial Growth as Determinant of Antibiotic Production with Biotic Elicitors Stimulation},
    journal = {Jurnal Kimia Sains dan Aplikasi},
  volume = {23},
    number = {3},
    year = {2020},
    keywords = {microbial activity; elicitor; antibiotic production; Penicillium sp. LBKURCC34; Staphylococcus aureus},
    abstract = {An antibiotic-resistant and multidrug-resistant (MDR) issue open the role of researchers to continue to search for natural potential as a source of new antimicrobials. One of the potential fungi isolates that can produce antimicrobial active compounds from Indonesian tropical peat soils is  Penicillium sp.  LBKURCC34. In this study, the production of antimicrobial compounds from local isolates was carried out by batch fermentation method in liquid media with the addition of biotic elicitors to increase the extraction activity and yield. This study aims to optimize the results based on the time the elicitor is added.  Staphylococcus aureus  was used as a biotic elicitor, which was added on days 2, 3, and 4 in the production of antibiotics by fermentation incubation of 6-14 days. The antibiotic production media was extracted with ethyl acetate and evaporated. The antimicrobial test was carried out by the disk diffusion method against pathogenic bacteria  Escherichia coli ,  Staphylococcus aureus ,  Bacillus subtilis , and  Staphylococcus epidermidis  using three crude extract contents (19; 38; and 57 µg/disc). Amoxicillin® was used as a positive control (10 µL/disc). The results showed that the addition of  S. aureus  biotic elicitor extended the log phase growth of the fungus  Penicillium sp.  LBKURCC34. The optimum condition of production was obtained by adding initiator treatment on the 3 rd  day for 14 days incubation with the highest yield and could inhibit the growth of all pathogenic microbes.},
   issn = {2597-9914},   pages = {89--95}  doi = {10.14710/jksa.23.3.89-95},
    url = {https://ejournal.undip.ac.id/index.php/ksa/article/view/27116}
}

Citation Format:

Abstract

An antibiotic-resistant and multidrug-resistant (MDR) issue open the role of researchers to continue to search for natural potential as a source of new antimicrobials. One of the potential fungi isolates that can produce antimicrobial active compounds from Indonesian tropical peat soils is Penicillium sp. LBKURCC34. In this study, the production of antimicrobial compounds from local isolates was carried out by batch fermentation method in liquid media with the addition of biotic elicitors to increase the extraction activity and yield. This study aims to optimize the results based on the time the elicitor is added. Staphylococcus aureus was used as a biotic elicitor, which was added on days 2, 3, and 4 in the production of antibiotics by fermentation incubation of 6-14 days. The antibiotic production media was extracted with ethyl acetate and evaporated. The antimicrobial test was carried out by the disk diffusion method against pathogenic bacteria Escherichia coli, Staphylococcus aureus, Bacillus subtilis, and Staphylococcus epidermidis using three crude extract contents (19; 38; and 57 µg/disc). Amoxicillin® was used as a positive control (10 µL/disc). The results showed that the addition of S. aureus biotic elicitor extended the log phase growth of the fungus Penicillium sp. LBKURCC34. The optimum condition of production was obtained by adding initiator treatment on the 3^rd day for 14 days incubation with the highest yield and could inhibit the growth of all pathogenic microbes.

Fulltext View|Download

Keywords: microbial activity; elicitor; antibiotic production; Penicillium sp. LBKURCC34; Staphylococcus aureus

Funding: Program Kreativitas Mahasiswa (PKM-PE) Belmawa Ristekdikti Tahun 2019; Penelitian Dasar Desentralisasi DRPM Kemenristekdikti Tahun 2019

Article Metrics:

Article Info

Section: 8th - International Conference of the Indonesian Chemical Society (ICICS)

Language : EN

In Vol 23, No 3 (2020): Volume 23 Issue 3 Year 2020

Recent articles

Antioxidant potential and identification of active compounds on Kabau seed (Archidendron bubalinum) flesh and husk extract Synthesis of Metal–Curcumin Complex Compounds (M = Na⁺, Mg²⁺, Cu²⁺) Synthesis and Characterization of Triamine modified coated Iron Sand Hybrid Nanomaterials originating from Kendal Coast α-Amylase Inhibition Activities by Flavonoid Compounds from Panda Plants (Kalanchoe tomentosa) Microbial Growth as Determinant of Antibiotic Production with Biotic Elicitors Stimulation More recent articles

Most cited articles

Polimerisasi Eugenol Dengan Katalis Asam Sulfat Pekat Dealuminasi Zeolit Alam Cipatujah Melalui Penambahan Asam dan Oksidator Utilization of Rice Husk Cellulose as a Magnetic Nanoparticle Biocomposite Fiber Source for the Absorption of Manganese (Mn2+) Ions in Peat Water Pengaruh Waktu pada Pembentukan Kalsium Fosfat dengan Sistem Membran Selulosa Bakterial Pemanfaatan Karbon Aktif Serbuk Gergaji Kayu Jati untuk Menurunkan Chemical Oxygen Demand (COD) Limbah Cair Industri Tekstil More cited articles

Dattu Singh, Vandana Rathod, Shivaraj Ninganagouda, Jyothi Hiremath, Ashish Kumar Singh and Jasmine Mathew, Optimization and characterization of silver nanoparticle by endophytic fungi Penicillium sp. isolated from Curcuma longa (turmeric) and application studies against MDR E. coli and S. aureus, Bioinorganic chemistry and applications, 2014, Article ID 408021, (2014), 1-8 https://doi.org/10.1155/2014/408021
Cheng Gu and K. G. Karthikeyan, Sorption of the Antibiotic Tetracycline to Humic-Mineral Complexes, Journal of Environmental Quality, 37, 2, (2008), 704-711 https://doi.org/10.2134/jeq2007.0030
Catherine M. Yule and Lalita N. Gomez, Leaf litter decomposition in a tropical peat swamp forest in Peninsular Malaysia, Wetlands Ecology and Management, 17, 3, (2009), 231-241 https://doi.org/10.1007/s11273-008-9103-9
WR Utamy, Isolasi fungi penghasil kitinase dari tanah hutan primer Pangkalan Bukit Cagar Biosfer Giam Siak Kecil - Bukti Batu Riau, Universitas Riau, 2012, Pekanbaru
Hendra Saputra, F Puspota and T Tjandrawati, Production of an antibacterial compound against the plant pathogen Erwinia carotovora subs. carotovora by the biocontrol strain Gliocladium sp. T.N.C73, International Journal of Agricultural Technology, 9, 5, (2013), 1157-1165
Annisa Fitri, Fraksinasi Metabolit Sekunder Dan Uji Antimikroba Media Fermentasi Batch Penicillium sp. LBKURCC34, Universitas Riau, 2019, Pekanbaru
Eka Rahayu Utami, Antibiotika, Resistensi, dan Rasionalitas Terapi, El-Hayah, 1, 4, (2012), 191-198 http://dx.doi.org/10.18860/elha.v1i4.1783
Kenneth Todar, Staphylococcus aureus and Staphylococcal Disease, in: Todar's Online Textbook of Bacteriology, University of Wisconsin, 2005,
Khairullinas, C Hardiyanti and AP Anugerah, Produksi antibiotik Penicillium sp. LBKURCC34 isolat lokal tanah gambut Riau dengan stimulasi bakteri Staphylococcus aureus, in, Universitas Riau, Pekanbaru, 2018,
Hee Jung Lee, Jeong Hyung Lee, Bang Yeon Hwang, Hang Sub Kim and Jung Joon Lee, Anti-angiogenic activities of gliotoxin and its methylthio-derivative, fungal metabolites, Archives of Pharmacal Research, 24, 5, (2001), 397 https://doi.org/10.1007/BF02975182
Raina M. Maier, Ian L. Pepper, Charles P. Gerba and Terry J. Gentry, Environmental Microbiology, 2nd edition ed., Elsevier Science, 2009
Kathleen Park Talaro and Barry Chess, Foundations in Microbiology: Basic Principles, 9th edition ed., McGraw-Hill Education, 2014
Lud Waluyo, Mikrobiologi Umum, Universitas Muhammadiyah Malang Press, Malang, 2019
Asep Awaludin Prihanto, Perbandingan aktivitas antibakteri Penicillium notatum ATCC 28089 dengan Penicillium sp. R1M yang diisolasi dari mangrove Sonneratia caseolaris, Jurnal Pengolahan Hasil Perikanan Indonesia, 15, 1, (2012), 66-70 https://doi.org/10.17844/jphpi.v15i1.5336
Supartono Supartono, Nanik Wijayati, Lina Herlina and Enny Ratnaningsih, Produksi Antibiotika Oleh Bacillus subtilis M10 Dalam Media Urea-Sorbitol, Reaktor, 13, 3, (2011), 185-193 https://doi.org/10.14710/reaktor.13.3.185-193
Vania Aprilina Tanuwijaya, Produksi Penisilin oleh Penicillium chrysogenum dengan Penambahan Fenilalanin, in, Program Studi Biologi, Fakultas Teknobiologi, Universitas Atma Jaya Yogyakarta, Yogyakarta, 2015,
Titania Tjandrawati Nugroho, Jasril, A.M Chainulfiffah, Saryono, M.R. Tanzil and Muzeliati, Perbandingan dua metode ekstraksi antibiotik dari media fermentasi Gliocladium sp. TNC73., Jurnal Natur Indonesia, 9, 1, (2006), 16-21
Meenakshi Thakur and Baldev Singh Sohal, Role of elicitors in inducing resistance in plants against pathogen infection: a review, ISRN biochemistry, 2013, Article ID 762412, (2013), 1-10 https://doi.org/10.1155/2013/762412
Indah Yulia Ningsih, Pengaruh Elisitor Biotik dan Abiotik pada Produksi Flavonoid Melalui Kultur Jaringan Tanaman, PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia), 11, 2, (2014), 117-132 http://dx.doi.org/10.30595/pji.v11i2.829

Last update:

No citation recorded.

Last update: 2024-12-23 19:12:57

No citation recorded.

As an article writer, the author has the right to use their articles for various purposes, including use by institutions that employ authors or institutions that provide funding for research. Author rights are granted without special permission.

Author who publishes a paper at JKSA has the broad right to use their work for teaching and scientific purposes without the need to ask permission, including: used for (i) teaching in the author's class or institution, (ii) presentation at meetings or conferences and distributing copies to participants ; (iii) training conducted by the author or author's institution; (iv) distribution to colleagues for research use; (v) use in the compilation of subsequent authors' works; (vi) inclusion in a thesis or dissertation; (vi) reuse of part of the article in another work (with citation); (vii) preparation of derivative works (with citation); (viii) voluntary posting on open websites operated by authors or author institutions for scientific purposes (follow the CC BY-SA License).

Authors and readers can copy and redistribute material in any media or format, and mix, modify, and build material for any purpose but they must provide appropriate credit (provide article citation or content), providing links to the license, and indicate if there are changes.

The authors submitting a manuscript do so on the understanding that if accepted for publication, copyright of the article shall be assigned to Jurnal Kimia Sains dan Aplikasi (JKSA). Copyright encompasses rights to reproduce and deliver the article in all form and media, including reprints, photographs, microfilms and any other similar reproductions, as well as translations.

Reproduce any part of this journal, its storage in the database or its transmission by all forms or media is permitted does not need for written permission from JKSA. However, it should be cited as an honor in academic manners

JKSA and the Chemistry Department of Diponegoro University and the Editor make every effort to ensure that there are no data, opinions, or false or misleading statements published in JKSA. However, the content of the article is the sole and exclusive responsibility of each author.

The Copyright Transfer Form can be downloaded here: [Copyright Transfer Form - Indonesian] [Copyright Transfer Form - English]. The copyright form should be signed originally and send to the Editor in the form of printed letters, scanned documents sent via email or fax.

Adi Darmawan, Ph.D (Editor in Chief)

Editor in chief of Jurnal Kimia Sains dan Aplikasi (JKSA)

Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Jl. Prof. Soedarto, Kampus Undip Tembalang, Semarang, Central Java, Indonesia 50275
Email: jksa@live.undip.ac.id