Department of Chemistry, Faculty of Science and Informatics, Jenderal Achmad Yani University, Indonesia
BibTex Citation Data :
@article{JKSA27120, author = {Lilis Aisyah and Delia Ilfani and Faridia Lestari and Yenny Yun}, title = {α-Amylase Inhibition Activities by Flavonoid Compounds from Panda Plants (Kalanchoe tomentosa)}, journal = {Jurnal Kimia Sains dan Aplikasi}, volume = {23}, number = {3}, year = {2020}, keywords = {Panda Plant; K. tomentosa; kaempferol; inhibition of the α-amylase enzyme}, abstract = { Diabetes mellitus (DM) is one of the major health problems. One way to deal with diabetes mellitus is by inhibiting the work of enzymes that hydrolyze carbohydrates, thereby reducing glucose absorption. The enzyme that plays a role in the breakdown of starch into simple sugars is the α-amylase. The genus Kalanchoe or Panda plant has been widely reported to contain a variety of secondary metabolites that have several activities such as antimalarial, antibacterial, antidiabetic, and anticancer potential. This study aims to isolate, determine the chemical structure, and test the inhibitory activity of α-amylase from the ethyl acetate fraction of K. tomentosa leaves. The isolation stage began with the maceration process with n-hexane and fractionated with ethyl acetate. Ethyl acetate extract was refracted using a gradient liquid vacuum chromatography (KCV) method ( n -hexane: ethyl acetate: methanol). Repeated gravity columns separated the fraction of KCV results and preparative thin-layer chromatography (TLC-P) to obtain isolate pure. Isolate pure was characterized using several spectroscopic methods, including UV-Vis, IR, NMR. Determination of the structure of the compound against isolate pure shows that this isolate is a 3,7,4’-trihydroxyflavonol or kaempferol compound. The results of the test activity of 3,7,4’-trihydroxyflavonol compounds in inhibiting the enzyme α-amylase in vitro showed an IC 50 value of 346 µU/mL. The results obtained indicate that the IC 50 value is higher than that of the Acarbose, which was 39.3 µU/mL. }, issn = {2597-9914}, pages = {96--101} doi = {10.14710/jksa.23.3.96-101}, url = {https://ejournal.undip.ac.id/index.php/ksa/article/view/27120} }
Refworks Citation Data :
Diabetes mellitus (DM) is one of the major health problems. One way to deal with diabetes mellitus is by inhibiting the work of enzymes that hydrolyze carbohydrates, thereby reducing glucose absorption. The enzyme that plays a role in the breakdown of starch into simple sugars is the α-amylase. The genus Kalanchoe or Panda plant has been widely reported to contain a variety of secondary metabolites that have several activities such as antimalarial, antibacterial, antidiabetic, and anticancer potential. This study aims to isolate, determine the chemical structure, and test the inhibitory activity of α-amylase from the ethyl acetate fraction of K. tomentosa leaves. The isolation stage began with the maceration process with n-hexane and fractionated with ethyl acetate. Ethyl acetate extract was refracted using a gradient liquid vacuum chromatography (KCV) method (n-hexane: ethyl acetate: methanol). Repeated gravity columns separated the fraction of KCV results and preparative thin-layer chromatography (TLC-P) to obtain isolate pure. Isolate pure was characterized using several spectroscopic methods, including UV-Vis, IR, NMR. Determination of the structure of the compound against isolate pure shows that this isolate is a 3,7,4’-trihydroxyflavonol or kaempferol compound. The results of the test activity of 3,7,4’-trihydroxyflavonol compounds in inhibiting the enzyme α-amylase in vitro showed an IC50 value of 346 µU/mL. The results obtained indicate that the IC50 value is higher than that of the Acarbose, which was 39.3 µU/mL.
Article Metrics:
Last update:
Kalanchoe tomentosa: Phytochemical Profiling, and Evaluation of Its Biological Activities In Vitro, In Vivo, and In Silico
Antimicrobial Activity of β-Sitosterol Isolated from Kalanchoe tomentosa Leaves Against Staphylococcus aureus and Klebsiella pneumonia
Bioactive Compounds from Kalanchoe Genus Potentially Useful for the Development of New Drugs
TDZ and 2,4-D on in vitro propagation of panda plant from leaf explants
Last update: 2026-09-12 00:49:22
As an article writer, the author has the right to use their article for various purposes, including use by institutions that employ the author or institutions that provide funding for the research. These author rights are granted without special permission.
Authors who publish a paper in JKSA have broad rights to use their work for teaching and scientific purposes without the need to request permission, including: (i) teaching in the author's class or institution; (ii) presenting the work at meetings or conferences and distributing copies to participants; (iii) using the work in training conducted by the author or the author's institution; (iv) distributing copies to colleagues for research purposes; (v) using the work in the compilation of subsequent works by the author; (vi) including the work in a thesis or dissertation; (vii) reusing part of the article in another work, with appropriate citation; (viii) preparing derivative works, with appropriate citation; and (ix) voluntarily posting the work on open websites operated by the author or the author's institution for scientific purposes, in accordance with the CC BY-SA License.
Authors and readers may copy and redistribute the material in any medium or format, and may remix, transform, and build upon the material for any purpose, provided that they give appropriate credit by citing the article or its content, provide a link to the license, and indicate if changes were made.
The authors submitting a manuscript do so on the understanding that, if accepted for publication, the copyright of the article shall be assigned to Jurnal Kimia Sains dan Aplikasi (JKSA). Copyright encompasses the rights to reproduce and distribute the article in all forms and media, including reprints, photographs, microfilms, and any other similar reproductions, as well as translations.
Reproducing any part of this journal, storing it in a database, or transmitting it in any form or medium is permitted without written permission from JKSA. However, the original source must be properly cited in accordance with academic conventions.
JKSA, the Chemistry Department of Diponegoro University, and the Editor make every effort to ensure that no inaccurate, false, or misleading data, opinions, or statements are published in JKSA. However, the content of each article is the sole and exclusive responsibility of its authors.
The Copyright Transfer Form can be downloaded here: [Copyright Transfer Form - Indonesian] [Copyright Transfer Form - English]. The copyright form should bear the authors' original signatures and be sent to the Editor as a printed document, a scanned document sent via email, or by fax.
Prof. Adi Darmawan, Ph.D (Editor in Chief)
Editor in chief of Jurnal Kimia Sains dan Aplikasi (JKSA)
Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University
Visitor: View My Stats
Jurnal Kimia Sains dan Aplikasi is indexed in:
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
