Antioxidant and Antibacterial Activity of Selected Indonesian Honey against Bacteria of Acne

Shafira Ananda Djakaria  -  Department of Chemistry, Faculty of Mathematics and Natural Science, IPB University, Indonesia
*Irmanida Batubara orcid scopus  -  Department of Chemistry, Faculty of Mathematics and Natural Science, IPB University, Indonesia
Rika Raffiudin orcid scopus  -  Department of Biology, Faculty of Mathematics and Natural Science, IPB University, Indonesia
Received: 8 Dec 2019; Revised: 4 Aug 2020; Accepted: 5 Aug 2020; Published: 31 Aug 2020; Available online: 13 Aug 2020.
Open Access Copyright 2020 Jurnal Kimia Sains dan Aplikasi
License URL: http://creativecommons.org/licenses/by-sa/4.0

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Section: 8th - International Conference of the Indonesian Chemical Society (ICICS)
Language: EN
Statistics: 408 143
Abstract
Honey has natural bacteriostatic and bactericide activities; thus, it could be active compounds as an antibacterial against Propionibacterium acnes, the main bacteria in acne forming state. Our study aims to determine phenolic and flavonoid contents (qualitative and quantitative), antioxidants, along with antibacterial activity against P. acnes of selected Indonesian honey. Honey used in this study, including honeybees and stingless bees, were collected from Sumbawa, Riau, Belitung, Sukabumi, Banyuwangi, Bogor, and South Sulawesi. In the method, the quantitative content of phenols and flavonoid were measured using a spectrophotometer. Further, DPPH (2, 2’-diphenylpicryl hydrazyl) and ABTS (2’-azino-bis (3- ethylbenzothiazoline-6-sulfonic acid) assays were used to measure the antioxidant activities, and subsequently microdilution method was applied to determine antibacterial activity. The results showed that Indonesian honey contains phenolics and flavonoids in the range of 49.5-67.3 μg Gallic Acid Equivalent (GAE)/g and 766.5-1509.5 μg Quercetin Equivalent (QE)/g, respectively. All honey samples also show antioxidant and antibacterial activities. Of note, Apis cerana honey from Banyuwangi has the highest antioxidant and antibacterial activity compared to others. It has the Inhibition Concentration (IC50), Trolox Equivalent Antioxidant Capacity, Minimum Inhibitory Concentration (MIC) and Minimum Bactericide Concentration (MBC) values of 59.85 mg/L, 122 μg Trolox/g, 125 mg/mL, and 500 mg/mL respectively. Our study reveals that Indonesian honey can inhibit and kill P. acnes as well. Nonetheless, the phenolic content of honey is not always linear with antioxidant and antibacterial activities.
Keywords: antibacterial; antioxidant; Indonesian honey; Propionibacterium acnes; phenolic

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  1. Farhat S. Daud, Gauri Pande, Mamta Joshi, Ruchita Pathak, Shubhangi Wankhede, 2013, A study of antibacterial effect of some selected essential oils and medicinal herbs against acne causing bacteria, International Journal of Pharmaceutical Science Invention, 2, 1, 27-34
  2. John A. West, Giuseppe C. Zuccarello, Joe Scott, Jeremy Pickett-Heaps, Gwang Hoon Kim, 2005, Observations on Purpureofilum apyrenoidigerum gen. et sp. nov. from Australia and Bangiopsis subsimplex from India (Stylonematales, Bangiophyceae, Rhodophyta), Phycological Research, 53, 1, 49-66 https://doi.org/10.1111/j.1440-183.2005.00373.x
  3. Eka Rahayu Utami, 2012, Antibiotika, resistensi, dan rasionalitas terapi, Sainstis, 1, 1, 124-138 https://doi.org/10.18860/sains.v0i0.1861
  4. Tahereh Eteraf-Oskouei, Moslem Najafi, 2013, Traditional and modern uses of natural honey in human diseases: a review, Iranian Journal of Basic Medical Sciences, 16, 6, 731-742
  5. Yugo Berri Putra Rio, Aziz Djamal, Asterina Asterina, 2012, Perbandingan efek antibakteri madu asli Sikabu dengan madu Lubuk Minturun terhadap Escherichia coli dan Staphylococcus aureus secara in vitro, Jurnal Kesehatan Andalas, 1, 2, 59-62
  6. Elin Julianti, Kasturi K. Rajah, Irda Fidrianny, 2017, Antibacterial Activity of Ethanolic Extract of Cinnamon Bark, Honey, and Their Combination Effects against Acne-Causing Bacteria, Scientia Pharmaceutica, 85, 2, 19 https://doi.org/10.3390/scipharm85020019
  7. Ahmed Moussa, Djebli Noureddine, Meslem Abdelmelek, Aissat Saad, 2012, Antibacterial activity of various honey types of Algeria against Pathogenic Gram–Negative Bacilli: Escherichia coli and Pseudomonas aeruginosa, Asian Pacific Journal of Tropical Disease, 2, 3, 211-214 https://doi.org/10.1016/S2222-1808(12)60048-6
  8. SangMi Han, KwangGil Lee, JooHong Yeo, HaJu Baek, Kwankyu Park, 2010, Antibacterial and anti-inflammatory effects of honeybee (Apis mellifera) venom against acne-inducing bacteria, Journal of Medicinal Plants Research, 4, 6, 459-464 https://doi.org/10.5897/JMPR09.427
  9. Friedrich Ruttner, 2013, Biogeography and Taxonomy of Honeybees, Springer Berlin Heidelberg, Berlin
  10. K. L. Boorn, Y. Y. Khor, E. Sweetman, F. Tan, T. A. Heard, K. A. Hammer, 2010, Antimicrobial activity of honey from the stingless bee Trigona carbonaria determined by agar diffusion, agar dilution, broth microdilution and time-kill methodology, Journal of Applied Microbiology, 108, 5, 1534-1543 https://doi.org/10.1111/j.1365-2672.2009.04552.x
  11. Nelky Suriawanto, Tri Atmowidi, Sih Kahono, 2017, Nesting sites characteristics of stingless bees (Hymenoptera: Apidae) in Central Sulawesi, Indonesia, Journal of Insect Biodiversity, 5, 10, 1-9 https://doi.org/10.12976/jib/2017.5.10
  12. Anne E. Dollin, Leslie J. Dollin, the late Sh Sakagami, ocirc, ichi F., 1999, Australian stingless bees of the genus Trigona (Hymenoptera: Apidae), Invertebrate Systematics, 11, 6, 861-896 https://doi.org/10.1071/IT96020
  13. Claus Rasmussen, Jennifer Thomas, C., Michael Engel, S., 2017, A New Genus of Eastern Hemisphere Stingless Bees (Hymenoptera: Apidae), with a Key to the Supraspecific Groups of Indomalayan and Australasian Meliponini, American Museum Novitates, 2017, 3888, 1-33 https://doi.org/10.1206/3888.1
  14. Mohd Izwan Zainol, Kamaruddin Mohd Yusoff, Mohd Yasim Mohd Yusof, 2013, Antibacterial activity of selected Malaysian honey, BMC Complementary and Alternative Medicine, 13, 1, 1-10 https://doi.org/10.1186/1472-6882-13-129
  15. Badan Standardisasi Indonesia, 2013, Madu, SNI 01-3545-2013, Jakarta
  16. J. B. Harborne, 1987, Metode Fitokimia, K. Padmawinata, Institut Teknologi Bandung, Bandung
  17. E. Apostolidis, C. M. Lee, 2010, In Vitro Potential of Ascophyllum nodosum Phenolic Antioxidant-Mediated α-Glucosidase and α-Amylase Inhibition, Journal of Food Science, 75, 3, H97-H102 https://doi.org/10.1111/j.1750-3841.2010.01544.x
  18. Seung-Hun Lee, Mayur R. Bafna, Shruti S. Sancheti, Sung-Yum Seo, 2011, Acetylcholineterase inhibitory and antioxidant properties of Rhododendron yedoense var. Poukhanense bark, Journal of Medicinal Plants Research, 5, 2, 248-254 https://doi.org/10.5897/JMPR.9000035
  19. Sri Purwanti, Wulan Tri Wahyuni, Irmanida Batubara, 2019, Antioxidant Activity of Daemonorops draco Resin, Jurnal Kimia Sains dan Aplikasi, 22, 5, 179-183 https://doi.org/10.14710/jksa.22.5.179-183
  20. Byong Won Lee, Jin Hwan Lee, Sang Wan Gal, Yea Hwang Moon, Ki Hun Park, 2006, Selective ABTS Radical-Scavenging Activity of Prenylated Flavonoids from Cudrania tricuspidata, Bioscience, Biotechnology, and Biochemistry, 70, 2, 427-432 https://doi.org/10.1271/bbb.70.427
  21. Irmanida Batubara, Tohru Mitsunaga, Hideo Ohashi, 2009, Screening antiacne potency of Indonesian medicinal plants: antibacterial, lipase inhibition, and antioxidant activities, Journal of Wood Science, 55, 3, 230-235 https://doi.org/10.1007/s10086-008-1021-1
  22. Bambang Sigit Amanto, Siswanti Siswanti, Angga Atmaja, 2015, Kinetika pengeringan temu giring (Curcuma heyneana valeton & van zijp) menggunakan cabinet dryer dengan perlakuan pendahuluan blanching, Jurnal Teknologi Hasil Pertanian, 8, 2, 107-114 https://doi.org/10.20961/jthp.v0i0.12900
  23. Olga Escuredo, Montserrat Míguez, Maria Fernández-González, M. Carmen Seijo, 2013, Nutritional value and antioxidant activity of honeys produced in a European Atlantic area, Food Chemistry, 138, 2, 851-856 https://doi.org/10.1016/j.foodchem.2012.11.015
  24. R. S. Gill, V. S. Hans, Sukhmeet Singh, Parm Pal Singh, S. S. Dhaliwal, 2015, A small scale honey dehydrator, Journal of Food Science and Technology, 52, 10, 6695-6702 https://doi.org/10.1007/s13197-015-1760-0
  25. Juan Carlos Sánchez-Rangel, Jorge Benavides, J. Basilio Heredia, Luis Cisneros-Zevallos, Daniel A. Jacobo-Velázquez, 2013, The Folin–Ciocalteu assay revisited: improvement of its specificity for total phenolic content determination, Analytical Methods, 5, 21, 5990-5999 http://dx.doi.org/10.1039/C3AY41125G
  26. Ichda Chayati, Isnatin Miladiyah, 2014, Kandungan komponen fenolat, kadar fenolat total, dan aktivitas antioksidan madu dari beberapa daerah di Jawa dan Sumatera, Media Gizi Mikro Indonesia, 6, 1, 11-24
  27. Andi Nilawati Usman, Yuliana Syam, Rosdiana Natzir, Sutji Pratiwi Rahardjo, Muhammad Hatta, Indah Raya, Yuyun Widaningsih, Andi Zulkifli Abdullah, Ainurafiq, 2016, Nutrient Content and pH of Honey Propolis Trigona from Masamba, South Sulawesi Indonesia, International Journal of Sciences: Basic and Applied Research, 26, 247-251
  28. Wilfred Vermerris, Ralph Nicholson, 2007, Phenolic Compound Biochemistry, Springer Netherlands
  29. Chia-Chi Chang, Ming-Hua Yang, Hwei-Mei Wen, Jiing-Chuan Chern, 2002, Estimation of total flavonoid content in propolis by two complementary colorimetric methods, Journal of Food and Drug Analysis, 10, 3, 178-182 https://doi.org/10.38212/2224-6614.2748
  30. Ustadi Ustadi, Lilik Eka Radiati, Imam Thohari, 2017, Komponen Bioaktif pada Madu Karet (Hevea brasiliensis) Madu Kaliandra (Calliandra callothyrsus) dan Madu Randu (Ceiba pentandra), Jurnal Ilmu dan Teknologi Hasil Ternak, 12, 2, 97-102 https://doi.org/10.21776/ub.jitek.2017.012.02.6
  31. Philip Molyneux, 2004, The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity, Songklanakarin Journal of Science and Technology, 26, 2, 211-219
  32. Jonathan Sarwono, 2009, Statistik Itu Mudah: Panduan Lengkap untuk Belajar Komputasi Statistik Menggunakan SPSS 16, Universitas Atma Jaya Yogyakarta, Yogyakarta
  33. Ningjian Liang, David D. Kitts, 2014, Antioxidant property of coffee components: assessment of methods that define mechanisms of action, Molecules, 19, 11, 19180-19208 https://doi.org/10.3390/molecules191119180
  34. Hasan A. Alzahrani, Laid Boukraa, Yuva Bellik, Fatiha Abdellah, Balkees A. Bakhotmah, Sevgi Kolayli, Huseyin Sahin, 2012, Evaluation of the antioxidant activity of three varieties of honey from different botanical and geographical origins, Glob J Health Sci, 4, 6, 191-196 https://doi.org/10.5539/gjhs.v4n6p191
  35. Shaaban H. Moussa, Ahmed A. Tayel, Ahmed A. Al-Hassan, A. Farouk, 2013, Tetrazolium/Formazan Test as an Efficient Method to Determine Fungal Chitosan Antimicrobial Activity, Journal of Mycology, 2013, Article ID 753692, 1-7 https://doi.org/10.1155/2013/753692
  36. Peter C. H. Hollman, Michel N. C. P. Bijsman, Yvonne Van Gameren, Else P. J. Cnossen, Jeanne H. M. De Vries, Martijn B. Katan, 1999, The sugar moiety is a major determinant of the absorption of dietary flavonoid glycosides in man, Free Radical Research, 31, 6, 569-573 https://doi.org/10.1080/10715769900301141