1Chemistry Department, Institut Teknologi Sumatera, Indonesia
2Biology Education Department, Universitas Islam Negeri Raden Intan, Indonesia
3Pharmacy Department, Institut Teknologi Sumatera, Indonesia
4 Biology Department, Institut Teknologi Sumatera, Indonesia
BibTex Citation Data :
@article{JKSA29276, author = {Fina Frima and Rina Satiyarti and Yulistia Anggraini and Erga Syafitri and Ika Rini}, title = {Characteristics of Raw-Starch Degrading Amylase Bacteria from Natar Hot Spring Lampung}, journal = {Jurnal Kimia Sains dan Aplikasi}, volume = {23}, number = {7}, year = {2020}, keywords = {α-amylase; raw starch; Panninobacter}, abstract = { Indonesia has a diversity of hot spring as a habitat of bacteria. One of the hot springs is Natar hot spring, Lampung. This study is to report the characteristics of a bacterium called Nat1 isolate that produces amylase to degrade raw starch from Natar hot spring. Water samples were taken from hot springs with a temperature of 45 ° C and a pH of 7.0. Nat1 was isolated by screening on the medium of Starch-Luria Bertani at 37 ° C. Its amylase-producing bacteria showed an optimum amylolytic activity of a crude enzyme of Nat1 isolate in soluble starch was 267.2774 U/mL at 60°C. Genotypic identification results using the 16S rRNA gene showed that the Nat1 isolate is identified as Panninobacter phragmatetus . A crude enzyme of Nat1 isolate showed a novel amylase ability and could degrade the raw starch substrates, such as corn and sago, with the amount of reducing sugar for each raw starch, 37.0688 µmol/mg, and 24.2697 µmol/mg . In conclusion, Nat1 amylase is potentially used in industry for its ability to degrade raw starch directly. }, issn = {2597-9914}, pages = {238--243} doi = {10.14710/jksa.23.7.238-243}, url = {https://ejournal.undip.ac.id/index.php/ksa/article/view/29276} }
Refworks Citation Data :
Indonesia has a diversity of hot spring as a habitat of bacteria. One of the hot springs is Natar hot spring, Lampung. This study is to report the characteristics of a bacterium called Nat1 isolate that produces amylase to degrade raw starch from Natar hot spring. Water samples were taken from hot springs with a temperature of 45°C and a pH of 7.0. Nat1 was isolated by screening on the medium of Starch-Luria Bertani at 37°C. Its amylase-producing bacteria showed an optimum amylolytic activity of a crude enzyme of Nat1 isolate in soluble starch was 267.2774 U/mL at 60°C. Genotypic identification results using the 16S rRNA gene showed that the Nat1 isolate is identified as Panninobacter phragmatetus. A crude enzyme of Nat1 isolate showed a novel amylase ability and could degrade the raw starch substrates, such as corn and sago, with the amount of reducing sugar for each raw starch, 37.0688 µmol/mg, and 24.2697 µmol/mg. In conclusion, Nat1 amylase is potentially used in industry for its ability to degrade raw starch directly.
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