Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University, Indonesia
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@article{JKSA3284, author = {Wuryanti Wuryanti}, title = {Amobilisasi Enzim Bromelin dari Bonggol Nanas dengan Bahan Pendukung (Support) Karagenan dari Rumput Laut (Euchema cottonii)}, journal = {Jurnal Kimia Sains dan Aplikasi}, volume = {9}, number = {3}, year = {2006}, keywords = {Bromelin; enzim; amobilisasi; aktivitas spesifik}, abstract = {Bromelin adalah enzim proteolitik yang terdapat pada tananam nanas (Ananas comosus L) Telah dilakukan amobilisasi bromelin dari bonggol nanas. Amobilisasi dilakukan dengan metode penjebakan dengan polimer karagenan. Penentuan unit aktifitas dengan substrat kasein dan penentuan kadar protein dengan metoda Lowry. Enzim bromelin dari bonggol nanas bebas mempunyai kondisi waktu inkubasi optimum 5 menit, suhu optimum 35 o C, pH optimum 6,0 dengan aktifitas spesifik 7,215 U/mg. Setelah amobilisasi enzim bromelin bonggol nanas amobil memiliki waktu inkubasi optimum 5 menit, suhu optimum 33 o C, pH optimum 6,5 dengan aktivitas spesifik 7,215 U/mg. Pada pemakaian pertama aktifitas spesifik 7,215 U/mg, pada pemakaian kedua 7,215 U/mg, pada pemakaian ketiga 7,020 U/mg. Stabilitas enzim bromelin bonggol nanas amobil dapat dipertahankan hingga dua kali pemakaian.}, issn = {2597-9914}, pages = {55--59} doi = {10.14710/jksa.9.3.55-59}, url = {https://ejournal.undip.ac.id/index.php/ksa/article/view/3284} }
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