Pengembangan Metode Penentuan Isoflavon Kadar Rendah dalam Limbah Cair Tahu Menggunakan Enzim NADH Oksidase

Purbowatiningrum Ria Sarjono; Hasim Danuri; Dyah Iswantini

doi:10.14710/jksa.7.1.17-21

DOI: https://doi.org/10.14710/jksa.7.1.17-21

Pengembangan Metode Penentuan Isoflavon Kadar Rendah dalam Limbah Cair Tahu Menggunakan Enzim NADH Oksidase

Purbowatiningrum Ria Sarjono¹

, Hasim Danuri²

, Dyah Iswantini³

¹Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University, Indonesia

²Department Biochemistry, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Indonesia

³Department of Chemistry, Bogor Agricultural University, Indonesia

Published: 1 Apr 2004.

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Abstract

Penentuan isoflavon asal limbah tahu telah dilakukan.Ekstrak kasar isoflavon asal limbah tahu diperoleh dengan cara menghidrolisis menggunakan HCl 4 N diperoleh rendemen sebanyak 4,81 g/l. Kadar isoflavon diukur menggunakan metode Graham (1999) yang sebelumnya dilakukan optimasi enzim NADH oksidase diperoleh panjang gelombang optimum pada 327 nm pada suhu 350C, pH 5, konsentrasi substrat optimum 100 μM, konsentrasi enzim optimum 0,3 U/ml. Kadar isoflavon dalam limbah tahu 0,123x103 ppm sedangkan kadar isoflavon kedelai sebesar 0,5921x103 ppm. Sehingga kadar isoflavon limbah tahu adalah sebesar 20% dibanding kadar isoflavon kedelai.

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Keywords: isoflavon; limbah tahu; NADH Oksidase

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Section: Research Articles

Language : ID

In Vol 7, No 1 (2004): Volume 7 Issue 1 Year 2004

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