DOI: https://doi.org/10.14710/jksa.24.2.62-69

Immobilization of Crude Polyphenol Oxidase Extracts from Apples on Polypyrrole as a Membrane for Phenol Removal

1Department of Chemistry, Faculty of Sciences and Informatics, Universitas Jenderal Achmad Yani, Indonesia

2Department of Chemistry, Faculty of Mathematics and Natural Sciences, Institut Teknologi Bandung, Indonesia

Received: 1 Nov 2020; Revised: 8 Mar 2021; Accepted: 8 Mar 2021; Published: 15 Mar 2021.
Open Access Copyright 2021 Jurnal Kimia Sains dan Aplikasi under http://creativecommons.org/licenses/by-sa/4.0.

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Abstract

This research aims to make a polypyrrole (PPy) membrane and crude extract of polyphenol oxidase (PPO) as a membrane of mPPy/PPO apple extracts. The membrane of PPy/PPO-apple extract has been synthesized by the electrodeposition method. The electrolyte composition consists of a mixture of 0.10-0.20 M pyrrole (Py) and 50-100% PPO apple extract, which is stable using 50 mM of phosphate buffer solution at pH 6.80-7.00 and room temperature. The electrodeposition process is used 400 mesh steel gauze anode ST-304 and carbon plate cathode. Electrodeposition is carried out at potential = 5.00-6.00 V; current = 0.02-0.25 A; the distance from both electrodes = 1.00-2.00 cm for 300-500 seconds. The results from the deposition of PPy/PPO apple extract of the anode are a membrane of mPPy/PPO-apple extract, with total enzyme activity (U) = (957,1441, 2287 and 1754) using 2.00-5.00 mM phenol as a substrate which is measured based on the UV-visible spectrophotometric method. PPy and mPPy/PPO-apple extracts were characterized by SEM and SEM-EDS. The membrane of mPPy/PPO-apple extract can be used to remove phenol in industrial wastewater samples is 50-65% with a filtration capacity of 500 mL for 2 hours.

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Keywords: mPPy/PPO-apple extract; phenol; PPO activity; membrane
Funding: Directorate General of Higher Education of the Republic of Indonesia/RISTEK-BRIN; Research Grant of Pemprinas MP3EI-2015; LPPM Universitas Jenderal Achmad Yani

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