DOI: https://doi.org/10.14710/jksa.24.2.43-50

Uric Acid Sensor Based on PEDOT:PSS Modified Screen-Printed Carbon Electrode Fabricated with a Simple Painting Technique

1Department of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University, Indonesia

2Department of Chemistry, Faculty of Mathematics and Natural Science, IPB University, Bogor, Indonesia

3Tropical Biopharmaca Research Center, IPB University,Bogor, Indonesia

4 Chemistry Department, Indonesian Defense University, Indonesia

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Received: 5 Dec 2020; Revised: 23 Feb 2021; Accepted: 6 Mar 2021; Published: 15 Mar 2021.
Open Access Copyright 2021 Jurnal Kimia Sains dan Aplikasi under http://creativecommons.org/licenses/by-sa/4.0.

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Abstract
A screen-printed carbon electrode is a suitable electrode for electrochemical sensors due to its simplicity and portability. This study aimed to fabricate a screen-printed carbon electrode modified with poly (3,4-ethylenedioxythiophene) polystyrene sulfonate (SPCE-PEDOT:PSS) to improve the electrochemical performance for uric acid detection. The SPCE was fabricated using a layer-by-layer painting process of conductive ink consisting of graphite as a conductive material, polystyrene as a polymeric binder, and dichloromethane solvent on a polyvinyl chloride paper substrate. The fabricated SPCE was then modified with PEDOT:PSS by a drop-casting method. The characterization of SPCE-PEDOT:PSS surface morphology was performed using the scanning electron microscopy technique. The SPCE-PEDOT:PSS provided an acceptable linearity (R2 = 0.9985, 0.9993, 0.9985), sensitivity (0.070, 0.015, 0.024 µA/µM), precision (%RSD = 2.70%, 2.89%, 2.40%), limit of detection (1.61 µM, 1.14 µM, 1.62 µM), and limit of quantitation (5.37 µM, 3.81 µM to 5.39 µM) in measurement of uric acid standard solution using cyclic voltammetry, amperometry, and differential pulse voltammetry techniques, respectively. The studies using SPCE-PEDOT:PSS indicated that the electrode could be applied in the electrochemical measurement of uric acid in the human urine sample.
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Keywords: Amperometry, electrode, fabrication, uric acid, voltammetry
Funding: The Ministry of Research and Technology, National Research and Innovation Agency of Republic Indonesia

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Section: Research Articles
Language : EN
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