Isolation, Structure Determination, and Cytotoxic Activity of Steroid Compound from The Stem Bark of Aglaia cucullata (Meliaceae)

Kindi Farabi; Desi Harneti; Nurlelasari Nurlelasari; Tri Mayanti; Rani Maharani; Unang Supratman

doi:10.14710/jksa.26.6.217-223

DOI: https://doi.org/10.14710/jksa.26.6.217-223

Isolation, Structure Determination, and Cytotoxic Activity of Steroid Compound from The Stem Bark of Aglaia cucullata (Meliaceae)

Kindi Farabi¹

, Desi Harneti¹

, Nurlelasari Nurlelasari¹

, Tri Mayanti¹

, Rani Maharani¹

, Unang Supratman²

¹Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jatinangor 45363, Indonesia

²Central Laboratory, Universitas Padjadjaran, Jatinangor 45363, Indonesia

Received: 12 Jul 2023; Revised: 30 Aug 2023; Accepted: 31 Aug 2023; Published: 30 Sep 2023.

BibTex Citation Data :

@article{JKSA56368,
    author = {Kindi Farabi and Desi Harneti and Nurlelasari Nurlelasari and Tri Mayanti and Rani Maharani and Unang Supratman},
    title = {Isolation, Structure Determination, and Cytotoxic Activity of Steroid Compound from The Stem Bark of Aglaia cucullata (Meliaceae)},
    journal = {Jurnal Kimia Sains dan Aplikasi},
  volume = {26},
    number = {6},
    year = {2023},
    keywords = {Steroid; Aglaia; Aglaia cucullata; cytotoxic activity},
    abstract = {Steroids are one of the secondary metabolite groups that are abundant in many organisms. In plants, this type of compound is called phytosterols. Phytosterols have been widely known to show many potential bioactivities such as anti-inflammatory, induced apoptosis, cytotoxic, anti-diabetic, angiogenic, and antioxidant. One of the sources of phytosterol compounds is the genus  Aglaia . As the largest genus in the Meliaceae family, the genus  Aglaia  contains many bioactive compounds, including steroids. This research reported the isolation, structural determination, and cytotoxic activity of steroid compounds from the stem bark of  Aglaia cucullata , one of the members of the  Aglaia  genus. Pure isolated steroid was obtained after maceration of dried stem bark with ethanol and partitioned based on difference polarity, continued by column chromatography. Spectroscopic methods, including HRMS, FTIR, 1D and 2D NMR, were used for structural determination. The compound structure identified as stigmast-5-en-3β-ol-3β-oleate was first isolated from this species. MCF-7 breast cancer cell, B16-F10 melanoma cell, and CV-1 normal fibroblast kidney cell were used to evaluate its cytotoxicity. Stigmast-5-en-3β-ol-3β-oleate displayed low cytotoxicity against those two cancer cells and a normal cell.},
   issn = {2597-9914},   pages = {217--223}  doi = {10.14710/jksa.26.6.217-223},
    url = {https://ejournal.undip.ac.id/index.php/ksa/article/view/56368}
}

Citation Format:

Abstract

Steroids are one of the secondary metabolite groups that are abundant in many organisms. In plants, this type of compound is called phytosterols. Phytosterols have been widely known to show many potential bioactivities such as anti-inflammatory, induced apoptosis, cytotoxic, anti-diabetic, angiogenic, and antioxidant. One of the sources of phytosterol compounds is the genus Aglaia. As the largest genus in the Meliaceae family, the genus Aglaia contains many bioactive compounds, including steroids. This research reported the isolation, structural determination, and cytotoxic activity of steroid compounds from the stem bark of Aglaia cucullata, one of the members of the Aglaia genus. Pure isolated steroid was obtained after maceration of dried stem bark with ethanol and partitioned based on difference polarity, continued by column chromatography. Spectroscopic methods, including HRMS, FTIR, 1D and 2D NMR, were used for structural determination. The compound structure identified as stigmast-5-en-3β-ol-3β-oleate was first isolated from this species. MCF-7 breast cancer cell, B16-F10 melanoma cell, and CV-1 normal fibroblast kidney cell were used to evaluate its cytotoxicity. Stigmast-5-en-3β-ol-3β-oleate displayed low cytotoxicity against those two cancer cells and a normal cell.

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Keywords: Steroid; Aglaia; Aglaia cucullata; cytotoxic activity

Funding: Universitas Padjadjaran under contract No. 2203/UN6.3.1/PT.00/2023

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Section: Research Articles

Language : EN

In Vol 26, No 6 (2023): Volume 26 Issue 6 Year 2023

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