skip to main content

Synthesis and Characterization of Fluorapatite-Copper(II) Oxide with Sol-Gel Method as an Antibacterial Biomaterial

Department of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University, Bogor, West Java, Indonesia

Received: 6 Dec 2023; Revised: 11 Apr 2024; Accepted: 16 Apr 2024; Published: 30 Apr 2024.
Open Access Copyright 2024 Jurnal Kimia Sains dan Aplikasi under http://creativecommons.org/licenses/by-sa/4.0.

Citation Format:
Cover Image
Abstract

One of the calcium phosphate compounds that can be used as an antibacterial material for coating dental implants is fluorapatite (FAp). This research aims to synthesize FAp at three different sintering temperatures (600, 800, and 1000°C), copper(II) oxide (CuO), and fluorapatite-copper(II) oxide (FAp-CuO) using the sol-gel method, and test the antibacterial properties of the synthesized products. The sol-gel technique proved successful in synthesizing FAp, with optimal results observed at a sintering temperature of 1000°C, achieving a crystallinity level of 90%. Analyses conducted using X-ray diffractometer, Fourier-transform infrared spectrometer, and scanning electron microscope-energy dispersive X-ray spectrometer revealed FAp as the dominating phase, exhibiting Ca/P and P/F ratios of 1.84 and 4.67, respectively. In FAp-CuO, replacing Ca2+ with Cu2+ ions lowered the average crystallite size, crystallinity, and Ca/P ratio. FAp, CuO, and FAp-CuO all displayed antibacterial activities against S. aureus and E. coli, with FAp-CuO having the maximum average inhibitory zone diameters of 0.243 and 1.397 mm, respectively.

Fulltext View|Download
Keywords: Antibacterial; fluorapatite-copper(II) oxide; sintering; sol- gel

Article Metrics:

  1. A. J. Festas, A. Ramos, J. P. Davim, Medical devices biomaterials – A review, Proceedings of the Institution of Mechanical Engineers, Part L: Journal of Materials: Design and Applications, 234, 1, (2020), 218-228 https://doi.org/10.1177/1464420719882458
  2. Arie Hardian, Rosalinawati Dewi, Jasmansyah Jasmansyah, Dani Gustaman Syarif, Anceu Murniati, Synthesis and Characterization of Fe-doped Hydroxyapatite/ZnO Nanocomposites Using the Coprecipitation Method from Processed Limestone, Jurnal Kimia Sains dan Aplikasi, 26, 10, (2023), 404-410 https://doi.org/10.14710/jksa.26.10.404-410
  3. S. Manafi, F. Mirjalili, S. Joughehdoust, Synthesis of FAp, Forsterite, and FAp/Forsterite Nanocomposites by Sol-gel Method, Advanced Ceramics Progress, 6, 2, (2020), 35-42 https://doi.org/10.30501/acp.2020.109549
  4. Leszek Borkowski, Agata Przekora, Anna Belcarz, Krzysztof Palka, Grzegorz Jozefaciuk, Tomasz Lübek, Mariusz Jojczuk, Adam Nogalski, Grazyna Ginalska, Fluorapatite ceramics for bone tissue regeneration: Synthesis, characterization and assessment of biomedical potential, Materials Science and Engineering: C, 116, (2020), 111211 https://doi.org/10.1016/j.msec.2020.111211
  5. Raheleh Nikonam M., S. K. Sadrnezhaad, Jalil Vahdati Khaki, Effect of Cu2+ ion on Biological Performance of Nanostructured Fluorapatite Doped with Copper, Scientia Iranica, 24, 6, (2017), 2845-2855 https://doi.org/10.24200/sci.2017.4314
  6. Seyedali Seyedmajidi, Maryam Seyedmajidi, Fluorapatite: A Review of Synthesis, Properties and Medical Applications vs Hydroxyapatite, Iranian Journal of Materials Science and Engineering, 19, 2, (2022), 1-20 https://doi.org/10.22068/ijmse.2430
  7. Dmitry Bokov, Abduladheem Turki Jalil, Supat Chupradit, Wanich Suksatan, Mohammad Javed Ansari, Iman H. Shewael, Gabdrakhman H. Valiev, Ehsan Kianfar, Nanomaterial by Sol-Gel Method: Synthesis and Application, Advances in Materials Science and Engineering, 2021, (2021), 5102014 https://doi.org/10.1155/2021/5102014
  8. Nittima Rungpin, Sorapong Pavasupree, Pattarapan Prasassarakich, Sirilux Poompradub, Production of nano-calcium carbonate from shells of the freshwater channeled applesnail, Pomacea canaliculata, by hydrothermal treatment and its application with polyvinyl chloride, Polymer Composites, 36, 9, (2015), 1620-1628 https://doi.org/10.1002/pc.23070
  9. Marta Kalbarczyk, Aleksandra Szcześ, Izolda Kantor, Zoltan May, Dariusz Sternik, Synthesis and Characterization of Calcium Phosphate Materials Derived from Eggshells from Different Poultry with and without the Eggshell Membrane, Materials, 15, 3, (2022), 934 https://doi.org/10.3390/ma15030934
  10. Sumathi Shanmugam, Buvaneswari Gopal, Copper substituted hydroxyapatite and fluorapatite: Synthesis, characterization and antimicrobial properties, Ceramics International, 40, 10, Part A, (2014), 15655-15662 https://doi.org/10.1016/j.ceramint.2014.07.086
  11. Ahmed Alhilou, Thuy Do, Laith Mizban, Brian H. Clarkson, David J. Wood, Maria G. Katsikogianni, Physicochemical and Antibacterial Characterization of a Novel Fluorapatite Coating, ACS Omega, 1, 2, (2016), 264-276 https://doi.org/10.1021/acsomega.6b00080
  12. Thi Ha Tran, Viet Tuyen Nguyen, Copper Oxide Nanomaterials Prepared by Solution Methods, Some Properties, and Potential Applications: A Brief Review, International Scholarly Research Notices, 2014, (2014), 856592 https://doi.org/10.1155/2014/856592
  13. Muhammad Imran Din, Farhan Arshad, Zaib Hussain, Maria Mukhtar, Green Adeptness in the Synthesis and Stabilization of Copper Nanoparticles: Catalytic, Antibacterial, Cytotoxicity, and Antioxidant Activities, Nanoscale Research Letters, 12, (2017), 638 https://doi.org/10.1186/s11671-017-2399-8
  14. Pachara Chalayon, Chanchana Tanwongsan, Antibacterial effects of copper microparticles/copper nanoparticles/copper (II) oxide nanoparticles and copper microparticles/copper nanoparticles/copper (I) oxide nanoparticles from ultrasono-electrochemical with hydrothermal assisted synthesis method, Engineering Journal, 25, 6, (2021), 55-66 https://doi.org/10.4186/ej.2021.25.6.55
  15. Shoaib Khan, Madiha Pirvani, Sadaf Humayoun, Omair Anjum, Saima Akram, Muhammad Asif Nathani, Synthesis of nano-hydroxyapatite and nano-fluoroapatite particles by sol-gel method, Pakistan Journal of Medicine Dentistry, 8, 2, (2019), 40-44
  16. Monika Patel, Sunita Mishra, Ruchi Verma, Deep Shikha, Synthesis of ZnO and CuO nanoparticles via Sol gel method and its characterization by using various technique, Discover Materials, 2, (2022), 1 https://doi.org/10.1007/s43939-022-00022-6
  17. Kamil Pajor, Lukasz Pajchel, Joanna Kolmas, Hydroxyapatite and Fluorapatite in Conservative Dentistry and Oral Implantology—A Review, Materials, 12, 17, (2019), 2683 https://doi.org/10.3390/ma12172683
  18. D. Kherifi, H. Belhouchet, S. Ramesh, K. Y. Sara Lee, A. Kenzour, S. Djoualah, M. K. G. Abbas, Y. H. Wong, S. Ramesh, Sintering behaviour of fluorapatite–silicate composites produced from natural fluorapatite and quartz, Ceramics International, 47, 12, (2021), 16483-16490 https://doi.org/10.1016/j.ceramint.2021.02.216
  19. Ningappa Horti, Mallappa Kamatagi, Ninganagouda Patil, Madivalagouda Sannaikar, Sanjeev Inamdar, Synthesis and optical properties of copper oxide nanoparticles: effect of solvents, Journal of Nanophotonics, 14, 4, (2020), 046010 https://doi.org/10.1117/1.JNP.14.046010
  20. A. Kazuz, Ž Radovanović, Dj Veljović, V. Kojić, V. Miletić, R. Petrović, Dj Janaćković, α-Tricalcium phosphate/fluorapatite based composite cements: Synthesis, mechanical properties, and biocompatibility, Ceramics International, 46, 16, (2020), 25149-25154 https://doi.org/10.1016/j.ceramint.2020.06.301
  21. Fariborz Sharifianjazi, Amirhossein Esmaeilkhanian, Mostafa Moradi, Amirhosein Pakseresht, Mehdi Shahedi Asl, Hassan Karimi-Maleh, Ho Won Jang, Mohammadreza Shokouhimehr, Rajender S. Varma, Biocompatibility and mechanical properties of pigeon bone waste extracted natural nano-hydroxyapatite for bone tissue engineering, Materials Science and Engineering: B, 264, (2021), 114950 https://doi.org/10.1016/j.mseb.2020.114950
  22. Linda Pastero, Marco Bruno, Dino Aquilano, About the Genetic Mechanisms of Apatites: A Survey on the Methodological Approaches, Minerals, 7, 8, (2017), 139 https://doi.org/10.3390/min7080139
  23. S. Prathap Selvaraj, Enhanced surface morphology of copper oxide (CuO) nanoparticles and its antibacterial activities, Materials Today: Proceedings, 50, (2022), 2865-2868 https://doi.org/10.1016/j.matpr.2020.09.574
  24. Rania Dadi, Rabah Azouani, Mamadou Traore, Christine Mielcarek, Andrei Kanaev, Antibacterial activity of ZnO and CuO nanoparticles against gram positive and gram negative strains, Materials Science and Engineering: C, 104, (2019), 109968 https://doi.org/10.1016/j.msec.2019.109968
  25. D. Magimai Antoni Raj, Amal George, A. Dhayal Raj, A. Albert Irudayaraj, X. Venci, J. Arumugam, S. John Sundaram, H. Joy Prabu, The role of pH in enhancing the capacity of CuO nanoparticles for antibacterial activity, Materials Today: Proceedings, 36, (2021), 504-508 https://doi.org/10.1016/j.matpr.2020.05.222
  26. R. Uma Maheswari, B. Jansi Rani, G. Ravi, R. Yuvakkumar, Fuad Ameen, A. Al-Sabri, Structural, morphological, optical and antibacterial properties of pentagon CuO nanoplatelets, Journal of Sol-Gel Science and Technology, 87, 3, (2018), 515-527 https://doi.org/10.1007/s10971-018-4773-0
  27. Clinical and Laboratory Standards Institute, Performance Standards for Antimicrobial Susceptibility Testing, 30th ed., 2011,

Last update:

No citation recorded.

Last update: 2024-06-14 21:29:48

No citation recorded.