Metabolite Formation of Pesticide Isoprocarb in Coffee Beans During Short-Term Storage and Condition

Rosaria Nainggolan; Asep Saefumillah; Endang Saefudin; Yanwar Pratama Putra

doi:10.14710/jksa.27.2.45-52

DOI: https://doi.org/10.14710/jksa.27.2.45-52

Metabolite Formation of Pesticide Isoprocarb in Coffee Beans During Short-Term Storage and Condition

Rosaria Nainggolan¹

, Asep Saefumillah¹

, Endang Saefudin¹

, Yanwar Pratama Putra²

¹Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Pondok Cina, Beji, Depok City, West Java 16424, Indonesia

²PT. DITEK JAYA, Kedoya Elok Plaza DA12, Panjang Street, Kebon Jeruk, West Jakarta, 11520, Indonesia

Received: 5 Jan 2024; Revised: 21 Feb 2024; Accepted: 26 Feb 2024; Published: 20 Mar 2024.

BibTex Citation Data :

@article{JKSA61250,
    author = {Rosaria Nainggolan and Asep Saefumillah and Endang Saefudin and Yanwar Pratama Putra},
    title = {Metabolite Formation of Pesticide Isoprocarb in Coffee Beans During Short-Term Storage and Condition},
    journal = {Jurnal Kimia Sains dan Aplikasi},
  volume = {27},
    number = {2},
    year = {2024},
    keywords = {carbamate; isoprocarb; o- cumenol; pesticide; toxicity; coffee beans},
    abstract = {Isoprocarb (IPC) is one of the most important carbamate pesticides for white flea control in coffee plants. The prevalence of isoprocarb pesticide residues in coffee, i.e., green coffee beans, is a cause for concern. Green coffee beans were intentionally contaminated with isoprocarb at concentrations >0.01 mg/kg, which was investigated in this research. Quantitative analysis of isoprocarb with the QuEChERS method and qualitative analysis for metabolite formation was characterized using UV-Vis, FTIR,  1 H-NMR, GC-MS, and LC-QTOF-MS. Based on the data, the metabolite formed is  o -cumenol through the hydrolysis reaction of  o -aryl carbamate with the enzyme carboxylesterase.  o -cumenol or 2- isopropylphenol as a phenol derivative. To verify the existence of metabolite analytes that are believed to be there in coffee tainted with isoprocarb, more reliable analytical techniques utilizing analytical standards must be developed. Isoprocarb concentrations decreased along the storage time, especially in unsterilized conditions. As a toxic compound, a quantitative structural activity relationship study (QSAR) was initially carried out through a software application for estimating toxicity (TEST) provided by the US Environmental Protection Agency (EPA). For additional information, based on the LC 50  and LD 50  data confirmed from the TEST application, it was concluded that isoprocarb is more toxic than  o -cumenol.},
   issn = {2597-9914},   pages = {45--52}  doi = {10.14710/jksa.27.2.45-52},
    url = {https://ejournal.undip.ac.id/index.php/ksa/article/view/61250}
}

Citation Format:

Abstract

Isoprocarb (IPC) is one of the most important carbamate pesticides for white flea control in coffee plants. The prevalence of isoprocarb pesticide residues in coffee, i.e., green coffee beans, is a cause for concern. Green coffee beans were intentionally contaminated with isoprocarb at concentrations >0.01 mg/kg, which was investigated in this research. Quantitative analysis of isoprocarb with the QuEChERS method and qualitative analysis for metabolite formation was characterized using UV-Vis, FTIR, ¹H-NMR, GC-MS, and LC-QTOF-MS. Based on the data, the metabolite formed is o-cumenol through the hydrolysis reaction of o-aryl carbamate with the enzyme carboxylesterase. o-cumenol or 2- isopropylphenol as a phenol derivative. To verify the existence of metabolite analytes that are believed to be there in coffee tainted with isoprocarb, more reliable analytical techniques utilizing analytical standards must be developed. Isoprocarb concentrations decreased along the storage time, especially in unsterilized conditions. As a toxic compound, a quantitative structural activity relationship study (QSAR) was initially carried out through a software application for estimating toxicity (TEST) provided by the US Environmental Protection Agency (EPA). For additional information, based on the LC₅₀ and LD₅₀ data confirmed from the TEST application, it was concluded that isoprocarb is more toxic than o-cumenol.

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Keywords: carbamate; isoprocarb; o- cumenol; pesticide; toxicity; coffee beans

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Section: Research Articles

Language : EN

In Vol 27, No 2 (2024): Volume 27 Issue 2 Year 2024

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