Production, Quality Test, and Activity Evaluation of Methanol Extract and Nanoparticles from Pluchea indica (L.) Leaves as Additives in Natural Soap against Propionibacterium acnes

Arsya Cahya Saputra; Elang Seta Wiratama; Fiyola Mayangsari; Via Divinka Oktafinantyas; Sri Handayani

doi:10.14710/jksa.27.2.91-100

DOI: https://doi.org/10.14710/jksa.27.2.91-100

Production, Quality Test, and Activity Evaluation of Methanol Extract and Nanoparticles from Pluchea indica (L.) Leaves as Additives in Natural Soap against Propionibacterium acnes

Arsya Cahya Saputra, Elang Seta Wiratama, Fiyola Mayangsari, Via Divinka Oktafinantyas, Sri Handayani

Department of Chemistry Education, Faculty of Mathematics and Natural Sciences, Universitas Negeri Yogyakarta, Sleman, Indonesia

Received: 22 Jan 2024; Revised: 15 Mar 2024; Accepted: 15 Mar 2024; Published: 20 Mar 2024.

BibTex Citation Data :

@article{JKSA61676,
    author = {Arsya Saputra and Elang Wiratama and Fiyola Mayangsari and Via Divinka Oktafinantyas and Sri Handayani},
    title = {Production, Quality Test, and Activity Evaluation of Methanol Extract and Nanoparticles from Pluchea indica (L.) Leaves as Additives in Natural Soap against Propionibacterium acnes},
    journal = {Jurnal Kimia Sains dan Aplikasi},
  volume = {27},
    number = {2},
    year = {2024},
    keywords = {Antibacterial activity; Natural soap; Propionibacterium acnes; Pluchea indica (L.) leaves; AgNPs},
    abstract = { Pluchea indica  (L.) leaves, both in the form of extracts and silver nanoparticles, are known to exhibit antibacterial activity, but they have not been widely utilized in the health sector, especially in dermatology. This study aims to determine the quality test and antibacterial evaluation of natural soap formulated with methanol extract and nanoparticles of  Pluchea indica  (L.) leaves against  Propionibacterium acnes . The synthesis of silver nanoparticles (AgNPs) was conducted using the reduction method, with AgNO 3  as a precursor and methanol extract of  Pluchea indica  (L.) leaves serving as a reducing agent or bioreductor. Natural soap was produced through the cold process method, with a curing period of 3 to 4 weeks. Natural soap, derived from herbal ingredients, was made by combining NaOH, essential oils, and additives such as methanol extract and silver nanoparticles from  Pluchea indica  (L.) leaves. Quality tests were performed to assess moisture content (MC), fatty acid content, and pH levels. Antibacterial activity was assessed utilizing the well-diffusion method, with various sample concentrations ranging from 1.0 g/mL to 0.0625 g/mL. The quality test results for all soap samples met the Indonesian National Standard (SNI) requirements for acidity, free fatty acids, and moisture content. The bar soap without additives exhibited antibacterial activity, with a maximum inhibition zone of 14.26 mm (at 0.25 g/mL) after 15 hours. Natural soap with methanol extract of  Pluchea indica  (L.) leaf demonstrated an inhibition zone of 11.49 mm (at 0.0625 g/mL) after 15 hours, while nanoparticle soap exhibited an inhibition zone of 12.77 mm (at 0.25 g/mL) after 12 hours. In summary, all soap samples showed antibacterial activity, indicating the potential of  Pluchea indica  (L.) leaves as effective antibacterial agents.},
   issn = {2597-9914},   pages = {91--100}  doi = {10.14710/jksa.27.2.91-100},
    url = {https://ejournal.undip.ac.id/index.php/ksa/article/view/61676}
}

Citation Format:

Abstract

Pluchea indica (L.) leaves, both in the form of extracts and silver nanoparticles, are known to exhibit antibacterial activity, but they have not been widely utilized in the health sector, especially in dermatology. This study aims to determine the quality test and antibacterial evaluation of natural soap formulated with methanol extract and nanoparticles of Pluchea indica (L.) leaves against Propionibacterium acnes. The synthesis of silver nanoparticles (AgNPs) was conducted using the reduction method, with AgNO₃ as a precursor and methanol extract of Pluchea indica (L.) leaves serving as a reducing agent or bioreductor. Natural soap was produced through the cold process method, with a curing period of 3 to 4 weeks. Natural soap, derived from herbal ingredients, was made by combining NaOH, essential oils, and additives such as methanol extract and silver nanoparticles from Pluchea indica (L.) leaves. Quality tests were performed to assess moisture content (MC), fatty acid content, and pH levels. Antibacterial activity was assessed utilizing the well-diffusion method, with various sample concentrations ranging from 1.0 g/mL to 0.0625 g/mL. The quality test results for all soap samples met the Indonesian National Standard (SNI) requirements for acidity, free fatty acids, and moisture content. The bar soap without additives exhibited antibacterial activity, with a maximum inhibition zone of 14.26 mm (at 0.25 g/mL) after 15 hours. Natural soap with methanol extract of Pluchea indica (L.) leaf demonstrated an inhibition zone of 11.49 mm (at 0.0625 g/mL) after 15 hours, while nanoparticle soap exhibited an inhibition zone of 12.77 mm (at 0.25 g/mL) after 12 hours. In summary, all soap samples showed antibacterial activity, indicating the potential of Pluchea indica (L.) leaves as effective antibacterial agents.

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Keywords: Antibacterial activity; Natural soap; Propionibacterium acnes; Pluchea indica (L.) leaves; AgNPs

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Section: Research Articles

Language : EN

In Vol 27, No 2 (2024): Volume 27 Issue 2 Year 2024

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Islan Nor, Siti Rahmita, Siti Nashihah, Potensi Antibakteri Ekstrak Etanol Daun Beluntas (Pluchea Indica L.) Terhadap Bakteri Staphylococcus epidermidis: Antibacterial Potential of Beluntas Leaf (Pluchea Indica L.) Ethanol Extract Against Staphylococcus epidermidis Bacteria, Medical Sains: Jurnal Ilmiah Kefarmasian, 7, 4, (2022), 725-734 https://doi.org/10.37874/ms.v7i4.469
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Susanty Susanty, Fairus Bachmid, Perbandingan metode ekstraksi maserasi dan refluks terhadap kadar fenolik dari ekstrak tongkol jagung (Zea mays L.), Jurnal Konversi, 5, 2, (2016), 87-92 https://doi.org/10.24853/konversi.5.2.87-92
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G. F. Brooks, K. C. Carroll, J. S. Butel, S. A. Morse, T. A. Mietzner, Jawetz, Melnick & Adelberg’s Medical Microbiology, 23 ed., McGraw-Hill Companies, 2010,
W. W. Davis, T. R. Stout, Disc plate method of microbiological antibiotic assay: I. Factors influencing variability and error, Applied Microbiology, 22, 4, (1971), 659-665 https://doi.org/10.1128/am.22.4.659-665.1971
G. J. Tortora, B. R. Funke, C. L. Case, Microbiology: An Introduction, Benjamin Cummings, San Francisco, 2010,
Nening Listari, Dini Yuliansari, Nurhidayatullah, Proses Pembuatan dan Pengujian Mutu Fisik Sabun Padat Dari Minyak Jelantah Dengan Ekstrak Daun Kelor, Jurnal Ilmiah Mandala Education, 8, 1, (2022), http://dx.doi.org/10.58258/jime.v8i1.2725
Indonesian National Standard, Sabun mandi padat, SNI 3532:2021, 2021
Fatridha Yansen, Vilma Humaira, Uji Mutu Sediaan Sabun Padat dari Ekstrak Lidah Buaya (Aloe vera), Jurnal Kesehatan Perintis, 9, 2, (2022), 82-88 https://doi.org/10.33653/jkp.v9i2.883
Sri Handayani, Indyah Sulistyo Arty, Cornelia Budimarwanti, Karim Theresih, Evy Yulianti, Melati Khairuddean, Preparation and antimicrobial activity analysis of organic soap Bar containing Gnetum gnemon Peel extract, Molekul, 16, 3, (2021), 226-234 http://dx.doi.org/10.20884/1.jm.2021.16.3.800
Azwar Agoes, Tanaman obat indonesia, 1st ed., Salemba Medika, Jakarta, 2010,
Syf. Octy Novy Fissy, Rafika Sari, Liza Pratiwi, Effectiveness of Anti Acne Gel Containing Ginger Ethanol Extract (Zingiber officinale Rosc.Var. Rubrum) against Propionibacterium acnes dan Staphylococcus epidermidis, Jurnal Ilmu Kefarmasian Indonesia, 12, 2, (2014), 194-201
Xiaoyu Chen, Yajun Lu, Mengyuan Shan, Hongyuan Zhao, Zhaoxin Lu, Yingjian Lu, A mini-review: mechanism of antimicrobial action and application of surfactin, World Journal of Microbiology and Biotechnology, 38, 8, (2022), 143 https://doi.org/10.1007/s11274-022-03323-3
Andi Ulfah Magefirah Rasyid, Zahira Amody, Pengujian efektifitas formula gel ekstrak daun beluntas (Pluchea indica (L.) Less) dengan variasi konsentrasi gelling agent sebagai kandidat sediaan anti jerawat, Jurnal Ilmiah Manuntung, 6, 2, (2020), 312-322 https://doi.org/10.51352/jim.v6i2.393
Hélène Omer, Andrew McDowell, Oleg A. Alexeyev, Understanding the role of Propionibacterium acnes in acne vulgaris: The critical importance of skin sampling methodologies, Clinics in Dermatology, 35, 2, (2017), 118-129 https://doi.org/10.1016/j.clindermatol.2016.10.003
Gianluigi Franci, Annarita Falanga, Stefania Galdiero, Luciana Palomba, Mahendra Rai, Giancarlo Morelli, Massimiliano Galdiero, Silver Nanoparticles as Potential Antibacterial Agents, Molecules, 20, 5, (2015), 8856-8874 https://doi.org/10.3390/molecules20058856
Michael A. Kohanski, Daniel J. Dwyer, James J. Collins, How antibiotics kill bacteria: from targets to networks, Nature Reviews Microbiology, 8, 6, (2010), 423-435 https://doi.org/10.1038/nrmicro2333

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