1Department of Biomedical Science, Faculty of Medicine, Universitas Andalas, Padang, Indonesia
2Faculty of Pharmacy, Universitas Andalas, Padang, Indonesia
3Research Center for Pharmaceutical Ingredients and Traditional Medicine, National Research and Innovation Agency, KST BJ. Habibie, Tangerang Selatan, Indonesia
4 Department of Biology, Faculty of Sciences and Mathematics, IPB University, Bogor, Indonesia
5 Department of Microbiology, Faculty of Medicine, Universitas Andalas, Padang, Indonesia
6 Department of Parasitology, Faculty of Medicine, Universitas Andalas, Padang, Indonesia
7 Department of Biochemistry, Faculty of Medicine, Universitas Andalas, Padang, Indonesia
BibTex Citation Data :
@article{JKSA66067, author = {Rafifa Ridha and Dira Hefni and Zetryana Puteri Tachrim and Gian Primahana and Jepri Agung Priyanto and Linosefa Linosefa and Adrial Adrial and Endrinaldi Endrinaldi and Muhammad Eka Prastya}, title = {Antibacterial Activity of Endophytic Bacterial Isolates from Kaempferia galanga Leaves}, journal = {Jurnal Kimia Sains dan Aplikasi}, volume = {27}, number = {11}, year = {2024}, keywords = {Antibacterial; Bacillus sp., Endophytic bacteria; Kaempferia galanga; Pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl)-}, abstract = { Indonesia has a high biodiversity, which can be relied upon as a potential source of medicinal materials. One of the medicinal plants in Indonesia is Kaempferia galanga , which demonstrates various pharmacological properties, including antibacterial, anti-inflammatory, antioxidant, anticancer, and antiangiogenic effects. However, directly extracting active compounds from plants requires a considerable amount of biomass. To address this challenge, utilizing endophytic bacteria associated with these plants presents a promising alternative. Consequently, the antibacterial activity of endophytic bacterial isolates from K. galanga leaves needs to be investigated. This study is an experimental laboratory investigation conducted in vitro. Two isolates demonstrated antibacterial activity: isolate code DR4 inhibited the growth of Escherichia coli (3 ± 0.5 mm), while isolate DR10 inhibited the growth of four bacteria: Bacillus subtilis (5 ± 0 mm), Staphylococcus aureus (1.5 ± 0.5 mm), Escherichia coli (1 ± 0 mm), and Pseudomonas aeruginosa (1 ± 0 mm). Disc diffusion tests using ethyl acetate extracts of isolate DR10 showed the highest antibacterial activity at a concentration of 10,000 ppm. The Minimum Inhibitory Concentration (MIC) was determined to be 156.2 ppm against B. subtilis , while the Minimum Bactericidal Concentration (MBC) was >625 ppm. Molecular identification showed that isolate DR 10 had 100% similarity to Bacillus sp. TS8. The active compound suspected to have antibacterial properties is pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl)-, with the highest abundance. }, issn = {2597-9914}, pages = {538--548} doi = {10.14710/jksa.27.11.538-548}, url = {https://ejournal.undip.ac.id/index.php/ksa/article/view/66067} }
Refworks Citation Data :
Indonesia has a high biodiversity, which can be relied upon as a potential source of medicinal materials. One of the medicinal plants in Indonesia is Kaempferia galanga, which demonstrates various pharmacological properties, including antibacterial, anti-inflammatory, antioxidant, anticancer, and antiangiogenic effects. However, directly extracting active compounds from plants requires a considerable amount of biomass. To address this challenge, utilizing endophytic bacteria associated with these plants presents a promising alternative. Consequently, the antibacterial activity of endophytic bacterial isolates from K. galanga leaves needs to be investigated. This study is an experimental laboratory investigation conducted in vitro. Two isolates demonstrated antibacterial activity: isolate code DR4 inhibited the growth of Escherichia coli (3 ± 0.5 mm), while isolate DR10 inhibited the growth of four bacteria: Bacillus subtilis (5 ± 0 mm), Staphylococcus aureus (1.5 ± 0.5 mm), Escherichia coli (1 ± 0 mm), and Pseudomonas aeruginosa (1 ± 0 mm). Disc diffusion tests using ethyl acetate extracts of isolate DR10 showed the highest antibacterial activity at a concentration of 10,000 ppm. The Minimum Inhibitory Concentration (MIC) was determined to be 156.2 ppm against B. subtilis, while the Minimum Bactericidal Concentration (MBC) was >625 ppm. Molecular identification showed that isolate DR 10 had 100% similarity to Bacillus sp. TS8. The active compound suspected to have antibacterial properties is pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl)-, with the highest abundance.
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