DOI: https://doi.org/10.14710/jksa.28.1.47-52

Synthesis and Characterization of Silver Nanoparticles-Chitosan Beads as Antibacterial Agents

1Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Negeri Semarang, Semarang, Indonesia

2Department of Chemistry Education, Faculty of Mathematics and Natural Sciences, Universitas Negeri Semarang, Semarang, Indonesia

Received: 18 Oct 2024; Revised: 25 Jan 2025; Accepted: 30 Jan 2025; Published: 31 Jan 2025.
Open Access Copyright 2025 Jurnal Kimia Sains dan Aplikasi under http://creativecommons.org/licenses/by-sa/4.0.

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Abstract
One of the key innovations in silver nanoparticle (AgNPs) material development is the formation of AgNPs/chitosan beads, which exhibit enhanced properties and broader functionality compared to chitosan or AgNPs alone. In this study, AgNPs/chitosan beads were synthesized using glutaraldehyde as a binding agent and sodium citrate as a reducing agent—both of which are safe and non-toxic, enabling broader applications. The synthesized beads were characterized to assess their chemical and physical properties, including functional groups, morphology, and crystallinity. Characterization results confirmed the successful cross-linking of chitosan by glutaraldehyde, enhancing the material’s physical and chemical strength. The chitosan beads appeared white, while AgNPs/chitosan beads were brownish, with an average bead size of 1.99 mm. X-ray diffraction analysis revealed that the silver in the chitosan beads exhibited a face-centered cubic crystal structure. Antibacterial testing demonstrated that AgNPs/chitosan beads exhibited superior antibacterial activity compared to chitosan beads, attributed to the release of Ag+ ions from the matrix.
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Keywords: beads; characterization; chitosan; silver nanoparticles; synthesis
Funding: Universitas Negeri Semarang under contract DIPA-023.17.2.677507/2022

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Section: Research Articles
Language : EN
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