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Mercury Determination Using Stannous Chloride Reductant Followed by Atomic Absorption Spectrometric Measurement: Performance Characteristics, Uncertainty Estimation, and Compliance Assessment

*Yohanes Susanto Ridwan orcid scopus  -  Indonesian Institutes of Sciences, Indonesia
Tiny Agustina Koesmawati  -  Indonesian Institutes of Sciences, Indonesia
Anna Edy Persulessy  -  Indonesian Institutes of Sciences, Indonesia
Raden Tina Rosmalina  -  Indonesian Institutes of Sciences, Indonesia
Astried Sunaryani  -  Indonesian Institutes of Sciences, Indonesia
Fitri Dara  -  Indonesian Institutes of Sciences, Indonesia

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Abstract

Indonesian government has committed to reduce and eliminate mercury. Hence, the intensity of monitoring activities of mercury levels in various matrices would be increased and supported by qualified analytical data. Key characteristic performances, i.e., the limit of detection, linear range, precision, trueness, have been successfully carried out, and the method was shown to fit the purpose. The limit detection, LoD and LoQ, were found to be 0.26 and 0.86 µg/L, respectively, which were adequate to reach the tightest regulatory limit of mercury in surface water (1 µg/L). The examined linearity range of 1-20 µg/L has been found sufficient for its application since a high mercury concentration in the typical sample is seldomly expected. Precision and trueness aspects of the method were shown to have satisfaction performance, with CV of 1,24% and recovery of 104.54%. All the possible uncertainty sources have been identified in this study. Since no reference material was available, the uncertainty of bias was evaluated through the recovery of the spiked sample. Compliance assessment to six measurement results has been performed; one result was below LoQ, four were clearly below regulatory limit, and one was questionable. Hence a decision rule was applied.

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Keywords: decision rule; mercury; performance characteristic, uncertainty

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  1. Agilent User Manual. 2017. Flame absorption spectrometry: Analytical methods. 4th edition, Agilent Technologies Australia (M) Pty. Ltd
  2. Al-Meer, S. et al. 2018. Validation of Total Mercury in Marine Sediment and Biological Samples, Using Cold Vapour Atomic Absorption Spectrometry. Methods and Protocols, 1(3), 1-14
  3. Bose-O'Reilly, S. et al. 2016. A preliminary study on health effects in villagers exposed to mercury in a small-scale artisanal gold mining area in Indonesia. Environmental Research, 149, 274–281
  4. Da Silva, D. G., Portugal, L.A.., Serra, A.M., Ferreira, S.L.C., Cerda, V. 2013. Determination of mercury in rice by MSFIA and cold vapor atomic fluorescence spectrometry. Food Chemistry, 137(1–4), 159–163
  5. Ellison, S.L.R. and Williams, A. 2ooo. Eurachem/CITAC Guide: Quantifying uncertainty in analytical measurement. 3rd edition
  6. Ellison, S.L.R. and Williams, A. 2007. Eurachem/CITAC Guide: Use of uncertainty information in compliance assessment, 1st edition
  7. Fernández, Z. H. et al. 2015. Application of Cold Vapor-Atomic Absorption (CVAAS) Spectrophotometry and Inductively Coupled Plasma-Atomic Emission Spectrometry methods for cadmium, mercury, and lead analyses of fish samples. Validation of the method of CVAAS. Food Contro, 48, 37–42
  8. Gao, C. and Huang, X. J. 2013. Voltammetric determination of mercury(II). TrAC - Trends in Analytical Chemistry, 51, 1–12
  9. Gibb, H. and O'Leary, K. G. 2014. Mercury exposure and health impacts among individuals in the artisanal and small-scale gold mining community: A comprehensive review'. Environmental Health Perspectives, 122(7), 667–672
  10. Guevara-Riba, A., Shuquillo, A., Lopez-Sanchez, J.F, Rubio, R. 2006. Comparison of three strategies to evaluate uncertainty from in-house validation data. A case study: Mercury determination in sediments. Analytical and Bioanalytical Chemistry, 385(7), pp. 1298–1303
  11. Hseu, Z. Y. 2004. Evaluating heavy metal contents in nine composts using four digestion methods. Bioresource Technology, 95(1), pp. 53–59
  12. ISO/IEC 17025. 2017. General requirements for the competence of testing and calibration laboratories. International Organization for Standardization (ISO), 3rd edition
  13. International vocabulary metrology – Basic and general concepts and associated terms (VIM). 2008. BIPM Joint Committee for Guides in Metrology Working Group 2, 3rd edition
  14. Koesmawati, T.A. et.al, 2021. Metode analisis merkuri dalam sedimen menggunakan reduktor natrium borohidrida. Indonesia Patent No. S00202106097
  15. Krisnayanti, B. D. et al. 2012. Assessment of environmental mercury discharge at a four-year-old artisanal gold mining area on Lombok Island, Indonesia. Journal of Environmental Monitoring, 14(10), 2598–2607
  16. Magnusson, B. and Ornemark, U. 2014. Eurachem Guide: The Fitness for Purpose of Analytical Methods – A Laboratory Guide to Method Validation and Related Topics, 2nd ed
  17. Mallongi, A., Pataranawat, P. and Parkpian, P. 2014. Mercury emission from artisanal buladu gold mine and its bioaccumulation in rice grains, Gorontalo Province, Indonesia. Advanced Materials Research, 931–932, 744–748
  18. Manzoori, J.L., Sorouraddin, M.H., Shabani, A.M.H. 1998. Determination of lead in different samples by atomic absorption spectrometry after preconcentration with dithizone immobilized on surfactant-coated alumina. Journal of Analytical Atomic Spectrometry, 13, pp. 305-308
  19. Maxwell, R. J., Pensabene, J. W. and Fiddler, W. 1993. Multiresidue recovery at PPB levels of 10 nitrosamines from frankfurters by supercritical fluid extraction. Journal of Chromatographic Science, 31(6), pp. 212–215
  20. Paez, V. et al. 2016. Journal of AOAC International, 99(4), pp. 1122–1124
  21. Pereyra, M. T., Lista, A. G. and Fernández Band, B. S. 2013. Quantifying uncertainty in mercury wastewater analysis at different concentration levels and using information from proficiency test with a limited number of participants. Talanta, 111, 69–75
  22. Singh, N., Ahuja, T., Ojha, V.N., Soni, D., Tripathy, S.S., Leito, I. 2013. Quantifying uncertainty in mercury measurement in suspended particulate matter using cold vapor technique using atomic absorption spectrometry with hydride generator. SpringerPlus, 2(1), 1–11
  23. Torres, D. P., Martins-Teixeira, M.B., Cadore, S., Queiroz, H.M. 2015. Method validation for control determination of mercury in fresh fish and shrimp samples by solid sampling thermal decomposition/amalgamation atomic absorption spectrometry. Journal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural Wastes, 50(7), 514–522
  24. Zhu, W., Sommar, J., Lin, C.J., Feng, X. 2015. Mercury vapor air-surface exchange measured by collocated micrometeorological and enclosure methods - Part II: Bias and uncertainty analysis. Atmospheric Chemistry and Physics, 15(10), 5359–5376

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