STUDI BACILLUS FIRMUS  SEBAGAI KANDIDAT PROBIOTIK DALAM MENGHADAPI Aeromonas hydrophila PADA MEDIA BUDIDAYA The Study of Bacillus firmus as Probiotic Candidate in Supressing Aeromonas hydrophila in Culture Media

Alfabetian Harjuno Condro Haditomo; Angela Mariana Lusiastuti; Widanarni Widanarni

doi:10.14710/ijfst.11.2.111-114

STUDI BACILLUS FIRMUS SEBAGAI KANDIDAT PROBIOTIK DALAM MENGHADAPI Aeromonas hydrophila PADA MEDIA BUDIDAYA The Study of Bacillus firmus as Probiotic Candidate in Supressing Aeromonas hydrophila in Culture Media

*Alfabetian Harjuno Condro Haditomo - Program Studi Budidaya Perairan Fakultas Perikanan dan Ilmu Kelautan , Indonesia

Angela Mariana Lusiastuti - Balai Riset Penelitian Budidaya Air Tawar Sempur Bogor, Indonesia

Widanarni Widanarni - Program Studi Budidaya Perairan Fakultas Perikanan dan Ilmu Kelautan, Indonesia

Published: 25 Feb 2016.

DOI: https://doi.org/10.14710/ijfst.11.2.111-114 View

BibTex Citation Data :

@article{IJFST11145,
author = {Alfabetian Harjuno Condro Haditomo and Angela Mariana Lusiastuti and Widanarni Widanarni},
title = {STUDI BACILLUS FIRMUS SEBAGAI KANDIDAT PROBIOTIK DALAM MENGHADAPI Aeromonas hydrophila PADA MEDIA BUDIDAYA The Study of Bacillus firmus as Probiotic Candidate in Supressing Aeromonas hydrophila in Culture Media},
journal = {Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology},
volume = {11},
number = {2},
year = {2016},
keywords = {Bacillus firmus; probiotik; Aeromonas hydrophila; media budidaya},
abstract = { Pengendalian penyakit bakterial yang umum dilakukan dengan pemakaian antibiotik atau bahan kimia sudah tidak diperbolehkan lagi karena menimbulkan patogen yang resisten terhadap bahan kimia tersebut, terlebih jika penggunaan tidak sesuai dengan anjuran yang diberikan. Dampak negatif terhadap kesehatan konsumen berupa residu antibiotik juga menjadi pertimbangan yang harus diperhatikan. Manipulasi terhadap populasi mikroba yang berada di perairan guna pencegahan sebelum terjadinya serangan bakteri yang bersifat mematikan perlu dilakukan sebagaimana konsep probiotik sebagai biokontrol. Tujuan penelitian ini adalah menguji kandidat probiotik dalam menekan atau menghambat bakteri patogen Aeromonas hydrophila. Penelitian ini dilaksananakan dalam dua tahap. Tahap pertama adalah tahap pengujian bakteri kandidat probiotik secara in vitro menggunakan metode zona hambat dan kultur bersama pada media agar. Tahap kedua adalah uji tentang bakteri kandidat probiotik dengan patogen pada media budidaya. Hasil terbaik penelitian tahap pertama pada uji kultur bersama antara kandidat probiotik B. firmus dengan A. hydrophila pada skala in vitro adalah dengan penambahan probiotik B. firmus sebanyak 10 8 cfu/ml. Sedangkan pada penelitian tahap kedua didapatkan hasil berturut-turut perlakuan D dengan tingkat kelangsungan hidup (SR) mencapai 90%, perlakuan C dengan SR 75%, perlakuan A dengan SR 50% dan perlakuan K dengan SR 50%. Controlling bacterial disease with the use of antibiotics or chemicals is no longer allowed as it results in pathogens that are resistant to the chemicals, especially when not in accordance with the recommendations provided. The negative impactsof the antibiotics residues on the consumers’ health also need to be considered. Manipulation of microbial populations present in the waters as preventation before the lethal attack of bacteria needs to be done which is in accordance with the concept of probiotics as biocontrol.The purpose of this study was to test the probiotic candidates in suppressing or inhibiting pathogenic bacteria Aeromonas hydrophila. This study was conducted in two stages. The first stage was to test a candidate probiotic bacteria in vitro using culture methods and inhibition zone on the media together. The second stage wasto test candidate probiotic bacteria to pathogens on the cultivation media. The best results in the first phase of the research is shared culture test between probiotic candidate B. FIRMUS with A. hydrophila on vitro scale is the addition of the probiotic B. FIRMUS 108 cfu / ml. While in the second phase of the research results obtained successively: treatment D with a survival rate (SR) reaches 90%, treatment C with SR 75%, treatment A with SR 50% and treatment K with SR 50%. },
issn = {2549-0885}, pages = {111--114} doi = {10.14710/ijfst.11.2.111-114},
url = {https://ejournal.undip.ac.id/index.php/saintek/article/view/11145}
}

Citation Format:

Abstract

Pengendalian penyakit bakterial yang umum dilakukan dengan pemakaian antibiotik atau bahan kimia sudah tidak diperbolehkan lagi karena menimbulkan patogen yang resisten terhadap bahan kimia tersebut, terlebih jika penggunaan tidak sesuai dengan anjuran yang diberikan. Dampak negatif terhadap kesehatan konsumen berupa residu antibiotik juga menjadi pertimbangan yang harus diperhatikan. Manipulasi terhadap populasi mikroba yang berada di perairan guna pencegahan sebelum terjadinya serangan bakteri yang bersifat mematikan perlu dilakukan sebagaimana konsep probiotik sebagai biokontrol. Tujuan penelitian ini adalah menguji kandidat probiotik dalam menekan atau menghambat bakteri patogen Aeromonas hydrophila. Penelitian ini dilaksananakan dalam dua tahap. Tahap pertama adalah tahap pengujian bakteri kandidat probiotik secara in vitro menggunakan metode zona hambat dan kultur bersama pada media agar. Tahap kedua adalah uji tentang bakteri kandidat probiotik dengan patogen pada media budidaya. Hasil terbaik penelitian tahap pertama pada uji kultur bersama antara kandidat probiotik B. firmus dengan A. hydrophila pada skala in vitro adalah dengan penambahan probiotik B. firmus sebanyak 10⁸ cfu/ml. Sedangkan pada penelitian tahap kedua didapatkan hasil berturut-turut perlakuan D dengan tingkat kelangsungan hidup (SR) mencapai 90%, perlakuan C dengan SR 75%, perlakuan A dengan SR 50% dan perlakuan K dengan SR 50%.

Controlling bacterial disease with the use of antibiotics or chemicals is no longer allowed as it results in pathogens that are resistant to the chemicals, especially when not in accordance with the recommendations provided. The negative impactsof the antibiotics residues on the consumers’ health also need to be considered. Manipulation of microbial populations present in the waters as preventation before the lethal attack of bacteria needs to be done which is in accordance with the concept of probiotics as biocontrol.The purpose of this study was to test the probiotic candidates in suppressing or inhibiting pathogenic bacteria Aeromonas hydrophila. This study was conducted in two stages. The first stage was to test a candidate probiotic bacteria in vitro using culture methods and inhibition zone on the media together. The second stage wasto test candidate probiotic bacteria to pathogens on the cultivation media. The best results in the first phase of the research is shared culture test between probiotic candidate B. FIRMUS with A. hydrophila on vitro scale is the addition of the probiotic B. FIRMUS 108 cfu / ml. While in the second phase of the research results obtained successively: treatment D with a survival rate (SR) reaches 90%, treatment C with SR 75%, treatment A with SR 50% and treatment K with SR 50%.

Keywords: Bacillus firmus; probiotik; Aeromonas hydrophila; media budidaya

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Section: Research Articles

Language: ID

In Vol 11, No 2 (2016): SAINTEK PERIKANAN

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IDENTIFIKASI DAN KELIMPAHAN BAKTERI PADA JENIS KARANG Acropora sp. DI REEF FLAT TERUMBU KARANG PULAU PANJANG JEPARA Identification and Abundance of Bacteria In Acropora sp. at Coral Reef Flat Panjang Island Jepara PENGGUNAAN PROBIOTIK GUNA PENINGKATAN PERTUMBUHAN, EFISIENSI PAKAN, TINGKAT KELULUSHIDUPAN DAN NILAI NUTRISI IKAN BANDENG (Chanos chanos) (Probiotic Use for Growth Improvement, Feed Efficiency, Survival Rate and Nutrition Value of Milkfish (Chanos chanos)) PENINGKATAN PERTUMBUHAN DAN IMUNITAS BENIH TERIPANG PASIR, Holothuria scabra DENGAN MELALUI PEMBERIAN PROBIOTIK DALAM PAKAN (Improvement of Growth and Immunity of Sandfish, Holothuria scabra Juvenile Through the use of Probiotics in Feed)

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