DOI: https://doi.org/10.14710/ijfst.15.1.60-65

EFEK PEMBERIAN PROBIOTIK TERHADAP IKAN KOI (Cyprinus carpio) YANG TERINFEKSI Myxobolus sp. (Effect of Probiotics Treatment on Koi Carp (Cyprinus carpio) Infected With Myxobolus sp.)

Nico Rahman Caesar  -  Program Studi Magister Budidaya Perairan, Fakultas Perikanan dan Ilmu Kelautan, Universitas Brawijaya, Indonesia
*Uun Yanuhar scopus  -  Fakultas Perikanan dan Ilmu Kelautan, Universitas Brawijaya, Indonesia
Muhammad Musa scopus  -  Fakultas Perikanan dan Ilmu Kelautan, Universitas Brawijaya, Indonesia

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Abstract

Myxobolus merupakan ektoparasit yang berbahaya dan dapat mengakibatkan kematian hingga 80%. Dalam praktek akuakultur, probiotik telah digunakan dalam beberapa tahun terakhir probiotik menjadi bagian integral dari praktik budaya untuk meningkatkan pertumbuhan dan ketahanan terhadap penyakit. Tujuan penelitian ini mengetahui pengaruh pemberian probiotik terhadap hematologi dan respon imun ikan koi yang terserang Myxobolus sp. yakni eritrosit dan leukosit serta ekspresi Nuclear Factor-kappa Beta  (NF-kB). Metode yang digunakan yaitu metode eksperimen. Dalam penelitian ini dibagi ke dalam 4 perlakuan yaitu, perlakuan (A) Kontrol, (B) ikan koi terinfeksi Myxobolus sp., (C) ikan koi terinfeksi Myxobolus sp. dengan pemberian probiotik dosis 0,55 ml dan (D) ikan koi terinfeksi Myxobolus sp. dengan pemberian probiotik dosis 1,1 ml. Metode pemberian perlakuan dengan menambahkan probiotik pada 30 liter air pada bak pemeliharaan. Kemudian dilakukan pengamatan eritrosit dan leukosit. Serta dilakukan Imunohistokimia menggunakan antibodi NF-kB pada jaringan insang. Berdasarkan hasil pengamatan eritrosit diperoleh nilai rata – rata pada perlakuan A sebesar 1.666.667 sel/mm3, perlakuan B sebesar 1.940.000 sel/mm3, perlakuan C sebesar 1.776.667 sel/mm3 dan perlakuan D sebesar 1.836.667 sel/mm3. Hasil pengamatan leukosit diperoleh nilai rata – rata pada perlakuan A sebesar 119.800 sel/mm3, perlakuan B sebesar 492.800 sel/mm3, perlakuan C sebesar 308.533 sel/mm3 dan perlakuan D sebesar 318.400 sel/mm3. Selanjutnya, berdasarkan hasil Imunohistokimia  didapatkan hasil pada perlakuan A nilai DAB sebesar 15,1%, perlakuan B nilai DAB sebesar 31,7%, perlakuan C nilai DAB sebesar 53,5% dan pada perlakuan D nilai DAB sebesar 47,5 %. Dari penelitian ini dapat disimpulkan bahwa pemberian probiotik berpengaruh terhadap eritrosit dan leukosit serta dapat meningkatkan ekspresi NF-KB sebagai respon imun pada ikan koi (Cyprinus carpio) yang terinfeksi Myxobolus sp. Dosis optimal pemberian probiotik yaitu pada dosis 0,55 ml.

 

Myxobolus is a dangerous parasite that can kill up to 80% on koi carp pond.. Probiotic intake has been proven to change the composition of the microbiota, and therefore helps in the recovery of microbiota that are disrupted (by antibiotics or other risk factors) into favorable or normal compositions. The purpose of this study was to determine the effect of probiotic administration on hematology and the immune response of koi fish attacked by Myxobolus sp. namely erythrocytes and leukocytes and expression of Nuclear Factor-kappa Beta (NF-kB). The method used was an experimental by providing probiotics to fish that promoted Myxobolus sp. In this study it was divided into 4 treatments namely, (A) Control, (B) koi carp infected by Myxobolus sp., (C) koi carp infected by Myxobolus sp. with probiotic doses of 0.55 ml and (D) koi fish infected by Myxobolus sp. by administering a probiotic dose of 1.1 ml. The treatment methods were the fish immersed into 30 L of water that added by Probiotic. The hematological observation of koi fish,  erythrocytes and leukocytes, was observed and immunohistochemistry using NF-KB antibodies in the gill tissue which is the target of the entry of Myxobolus sp. on Koi fish. Based on the results of the treatment of erythrocytes obtained the value of A handling is 1,666,667 cells / mm3, handling B is 1,940,000 cells / mm3, handling C is 1,776,667 cells / mm3 and handling D is 1,836,667 cells / mm3. The results of leukocyte observations obtained an average value on treatment A of 119,800 cells / mm3, treatment B was 492,800 cells / mm3, treatment C was 308,533 cells / mm3 and treatment D was 318,400 cells / mm3. Furthermore, based on the results of Immunohistochemistry, the results of handling A DAB value of 15.1%, handling B DAB value of 31.7%, handling C DAB value of 53.5% and at the time of treatment D DAB value of 47.5%. From this study it can be concluded that the treatment of probiotics affects erythrocytes and leukocytes and can increase the expression of NF-KB as an immune response in koi fish (Cyprinus carpio) infected with Myxobolus sp. The optimal dose of probiotics is at a dose of 0.55 ml 

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Keywords: Probiotik; Cyprinus carpio; Myxobolus sp.; Eritrosit; Leukosit; Imunohistokimia; NF-KB

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  1. Abbas AK and Lichtman AH (2007). Celluler and molecular immunology. 6th ed Philadelphia. WB Saunders. p. 34–37, 57–68
  2. Abraham, T.J., Babu, S., Mondal, S. and Banerjee, T., 2007. Effects of dietary supplementation of commercial human probiotic and antibiotic on the growth rate and content of intestinal microflora in ornamental fishes. Bangladesh Journal of Fisheries Research, 11(1), pp.57-63
  3. Albensi, B.C., M.P. Mattson, 2000. "Evidence for the involvement of TNF and NF-κB in hippocampal synaptic plasticity". Synapse, 35(2): 151-9
  4. Alifuddin, M., Y. Hadiroseyani dan I. Ohoiulun. 2003. Parasit pada Ikan Hias Air Tawar (Ikan Cupang, Gapi dan Rainbow). Akuakultur, 2 (2) : 93-100
  5. Aly, S.M., Ahmed, Y.A.G., Ghareeb, A.A.A. and Mohamed, M.F., 2008. Studies on Bacillus subtilis and Lactobacillus acidophilus, as potential probiotics, on the immune response and resistance of Tilapia nilotica (Oreochromis niloticus) to challenge infections. Fish & shellfish immunology, 25(1-2), pp.128-136
  6. Balcazar JL, Vendrell D, de Blas I, Ruiz-Zarzuela I, Giron´es O & Muzquiz JL (2007) In vitro competitive adhesion and production of antagonistic compounds by lactic acid bacteria against fish pathogens. Vet Microbiol 122: 373–380
  7. Blaxhall, P.C. and Daisley, K.W., 1973. Routine haematological methods for use with fish blood. Journal of fish biology, 5(6), pp.771-781
  8. Brunt, J., Newaj‐Fyzul, A. and Austin, B., 2007. The development of probiotics for the control of multiple bacterial diseases of rainbow trout, Oncorhynchus mykiss (Walbaum). Journal of Fish Diseases, 30(10), pp.573-579
  9. Christensen, H.R., Frøkiær, H. and Pestka, J.J., 2002. Lactobacilli differentially modulate expression of cytokines and maturation surface markers in murine dendritic cells. The Journal of Immunology, 168(1), pp.171-178
  10. Insivitawati, E., Mahasri, G. and Kusnoto, K., 2019. Gambaran Darah dan Histopatologi Insang, Usus Dan Otak Ikan Koi (Cyprinus carpio Koi) yang Diinfeksi Spora Myxobolus koi secara Oral [Haematology and Histopatology of Gills, Intestine And Brain Koi Fish (Cyprinus carpio Koi) Myxobolus koi Orally Infected]. Jurnal Ilmiah Perikanan dan Kelautan, 7(2), pp.225-234
  11. Kim, D.H. and Austin, B., 2006. Cytokine expression in leucocytes and gut cells of rainbow trout, Oncorhynchus mykiss Walbaum, induced by probiotics. Veterinary immunology and immunopathology, 114(3-4), pp.297-304
  12. Kim, D.H. and Austin, B., 2006. Cytokine expression in leucocytes and gut cells of rainbow trout, Oncorhynchus mykiss Walbaum, induced by probiotics. Veterinary immunology and immunopathology, 114(3-4), pp.297-304
  13. Lagler KF, Bardach JE, RR Miller, Passino DRM. 1977. Ichthyology. New YorkLondon: John Willey and Sons. Inc
  14. Lom, J. and I. Dykova. 2006. Myxozoan Genera : Definition and Notes Taxonomy, Life Cycle Terminology and Pathogenic Species. Folia Parasitologica, 53 : 1-36
  15. Mahasri, G. dan Kismiyati. 2011. Buku Ajar Parasit dan Penyakit Ikan I (Ilmu Penyakit Protozoa pada Ikan dan Udang). Universitas Airlangga. Surabaya. hal. 3-4
  16. Mahasri, G., 2017, February. Development of Spore Protein of Myxobolus koi as an Immunostimulant for Prevent of Myxobolusis on Gold Fish (Cyprinus carpio Linn) by Oral Immunisation. In IOP Conference Series: Earth and Environmental Science (Vol. 55, No. 1, p. 012009). IOP Publishing
  17. Moyle PB dan Cech Jr JJ. 2004. Fishes. An Introduction to Ichthyology. 5th ed. USA: Prentice Hall, Inc
  18. Niers, L.E., Timmerman, H.M., Rijkers, G.T., van Bleek, G.M., van Uden, N.O., Knol, E.F., Kapsenberg, M.L., Kimpen, J.L. and Hoekstra, M.O., 2005. Identification of strong interleukin‐10 inducing lactic acid bacteria which down‐regulate T helper type 2 cytokines. Clinical & Experimental Allergy, 35(11), pp.1481-1489
  19. Nikoskelainen S, Ouwehand AC, Bylund G, Salminen S, Lilius E-M (2003) Immune enhancement in rainbow trout (Oncorhynchus mykiss) by potential probiotic bacteria (Lactobacillus rhamnosus). Fish Shellfish Immunol 15:443–452
  20. Nikoskelainen S, Ouwehand AC, Bylund G, Salminen S, Lilius E-M 2003. Immune enhancement in rainbow trout (Oncorhynchus mykiss) by potential probiotic bacteria (Lactobacillus rhamnosus). Fish Shellfish Immunol 15:443–452
  21. Ramos-Vara, J. A. 2005. Technical Aspects of Immunohistochemistry. Vet Pathol 42:405–426
  22. Robertson PAW, O’Dowd C, Burrells C, Williams P, Austin B (2000). Use of Carnobacterium sp. as a probiotic for Atlantic salmon (Salmo salar L.) and rainbow trout (Oncorhynchus mykiss, Walbaum). Aquaculture 185:235–243
  23. Rosita., A. Mangalik., M. Adriani., dan M. Mahbub. 2012. Identifikasi dan Potensi Parasit pada Sumber Daya Ikan Hias di Danau Lais Kalimantan Tengah. EnviroScienteae, 8 : 164-174
  24. Salasia, S.I.O., D. Sulanjari, A. Ratnawati. 2001. Studi hematologi ikan air tawar. Biologi 2(12): 710-723
  25. Secombes, C. J., Wang, T. 2012. The innate and adaptive immune system of fish. Infectious disease in aquaculture. Woodhead Publishing Limited
  26. Syawal H, Syaifriadiman, Syauqi Hidayah. 2008. Pemberian Ekstrak Kayu Siwak (Salvadora Persica L.) untuk Meningkatkan Kekebalan Ikan Mas (Cyprinus carpio L.) yang Dipelihara dalam Keramba. ISSN : 1412-033X Januari 2008
  27. Tuominen V. J., Ruotoistenmäki S., Viitanen A., Jumppanen M., and Isola J. 2010. ImmunoRatio: a publicly available web application for quantitative image analysis of estrogen receptor (ER), progesterone receptor (PR), and Ki-67. Breast Cancer Research 2010, 12:R56
  28. Verschuere L, Rombaut G, Sorgeloos P, Verstraete W. 2000. Probiotic bacteria as biological control agents in aquaqulture. Microbiol Mol Biol Rev 64:655-671
  29. von der Weid, T., Bulliard, C. and Schiffrin, E.J., 2001. Induction by a lactic acid bacterium of a population of CD4+ T cells with low proliferative capacity that produce transforming growth factor β and interleukin-10. Clinical and diagnostic laboratory immunology, 8(4), pp.695-701
  30. Wulandari, R., 2017. Pengaruh Pemberian Probiotik Terhadap Aktivitas Letupan Respirasi Leukosit dalam Darah Ikan Nila (Oreochromis niloticus). Intek Akuakultur. 1(1) 71-76
  31. Yuasa, K., Panigoro, N., Bahnan, M., dan Khiidin., B. E. 2003. Panduan Diagnostik Penyakit Ikan. Teknik Diagnosa Penyakit Ikan Budidaya Air Tawar. Balai Budidaya Tawar Jambi
  32. Yuliono, D. T. 2012. Prevalensi Myxobolus dan Hubungan Korelasinya dengan Jumlah Populasi Oligochaeta yang Berpotensi Sebagai Inang Antara Myxobolus pada Ikan Koi (Cyprinus carpio) di Sentra Budidaya Ikan Koi Kabupaten Blitar, Jawa Timur.Budidaya Perairan. Fakultas Perikanan dan Kelautan. Universitas Airlangga. Surabaya. 60 hal

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