DOI: https://doi.org/10.14710/ijfst.16.1.51-58

POTENSI EPIBIOTIK CAMPURAN EKSTRAK DAUN BINAHONG (Anredera cordifolia) DAN TEMULAWAK (Curcuma zanthorrhiza) PADA PAKAN UNTUK MENGATASI INFEKSI Aeromonas hydrophila PADA IKAN LELE (Clarias gariepinus)

*Sarjito - Sarjito scopus  -  Diponegoro University, Indonesia
Slamet Budi Prayitno  -  Departemen Akuakultur,, Indonesia
Nida Qolbi Salma Rochani  -  Departemen Akuakultur,, Indonesia
Alfabetian Harjuno Condro Haditomo  -  Departemen Akuakultur,, Indonesia
Rosa Amalia  -  Departemen Akuakultur,, Indonesia
Desrina Desrina  -  Departemen Akuakultur,, Indonesia

Citation Format:
Abstract

Salah satu permasalahan pada budidaya ikan lele adalah Aeromonasis yang disebabkan oleh Aeromonas hydrophila. Berbagai upaya pencegahan dan pengobatan telah dilakukan dengan menggunakan bahan kimia maupun herbal. Bahan herbal, berupa epibiotik (tunggal maupun campuran) digunakan oleh pembudidaya untuk pencegahan dan pengobatan penyakit ini, karena mudah diperoleh, murah dan ramah lingkungan. Penelitian ini bertujuan untuk mengkaji pengaruh penambahan campuran epibiotik, ekstrak daun binahong dan temulawak pada pakan terhadap profil darah dan kelulushidupan ikan lele yang diinfeksi A. hydrophila. Metoda yang digunakan adalah eksperimen dengan Rancangan Acak Lengkap (6 perlakuan dan 3 ulangan). Seratus delapan puluh ikan lele uji dengan panjang 7-9 cm yang dipelihara pada akuarium berisi air 10 L.  Dosis campuran epibiotik, esktrak daun binahong dan temulawak menggunakan perbandingan untuk perlakuan A (0%:0%), B (100%:0%), C (75%:25%), D (50%:50%), E (25%:75%) dan F (0%:100%) dengan dosis dasar 2500 ppm untuk ekstrak daun binahong dan 900 ppm untuk temulawak. Campuran epibiotik tersebut ditambahkan pada pakan komersil sebagai pakan uji dengan metode spray. Pakan uji diberikan selama 14 hari, kemudian pada hari kelimabelas ikan uji diinjeksi A. hydrophila secara intramuscular dengan konsentrasi 106 CFU/mL. Hasil penelitian menunjukkan bahwa gejala klinis ikan lele yang terinfeksi A. hydrophila adalah nafsu makan rendah, bercak merah, luka, haemorhagi serta warna tubuh memucat.  Penambahan campuran epiobiotik ekstrak daun binahong dan temulawak berpengaruh nyata (p<0,05) terhadap profil darah dan kelulushidupan ikan uji pasca perlakuan dan pasca infeksi. Campuran epibiotik D mampu melawan infeksi A.hydrophilla pada C. gariepinus dengan tingkat kelulushidupan tertinggi (90±17%).

 

One of the problems in catfish culture was aeromonasis that was caused by Aeromonas hydrophila. The prevention and threatment of this disease have been carried out with using chemichal substance and an epibiotics from eco-friendly herbal plant extracts. Epibiotics, such as binahong leaves and curcuma extracts had been applicated by farmers to threat this disease because of it’s cheap and easy to get it. The aims of this study was to evaluate the effect of mixture binahong leaves and curcuma extracts in feed on blood profile and survival rate of catfish infected A. hydrophila. The method of research used was Completely Randomized Design, consisted of 6 treatments and 3 replications. The catfish used was 180 fishes with length of 7-9 cm that were cultured in aquarium with 10L waters. The basic dosage of binahong leaves and curcuma extracts used was 2500 ppm and 900 ppm with the ratio of treatment A (0%:0%), B (100%:0%), C (75%:25%), D (50%:50%), E (25%:75%), and F (0%:100%). The mix extract was added to the commercial feed as a feed test with spray methods. The treatment feed was given for 14 days and on the next day was done infected A. hydrophila intramusculary with density of 106 CFU/mL. The result showed that catfish infected A. hydrophila had low appetite, redness, ulcer, and hemorhagic, pale body. Feeding with the treatment feed showed the significant result on catfish’s blood profile and survival rate post-treatments and post-infection  (p<0.05). Treatment D showed the best result on survival rate (90±17%).

Fulltext View|Download
Keywords: Aeromonas hydrophila; binahong; lele; temulawak

Article Metrics:

Article Info
Section: Research Articles
Language : ID
Recent articles
RASIO GAYA APUNG DAN GAYA TENGGELAM PURSE SEINE TIPE LENGKUNG PADA KAPAL UKURAN DIBAWAH 20 GT DI PPP BULU, TUBAN (Ratio of Buoyancy and Sinking Force of Purse Seine with Curved Type on Vessels Under 20 GT in PPP Bulu, Tuban) POTENSI EPIBIOTIK CAMPURAN EKSTRAK DAUN BINAHONG (Anredera cordifolia) DAN TEMULAWAK (Curcuma zanthorrhiza) PADA PAKAN UNTUK MENGATASI INFEKSI Aeromonas hydrophila PADA IKAN LELE (Clarias gariepinus) KETERSEDIAAN AREA KERJA PADA EKS-KAPAL CANTRANG UNTUK MENGOPERASIKAN JARING INSANG OSEANIK (The Availability of working areas on the Ex-Cantrang Vessels to operate the Oceanic Gills Net) More recent articles
Most cited articles
Effect of Bakers Yeast, Vitamin B12, and Vitamin C as Nutritional Improvement of Food on the Density Production of Brachionus plicatilis Fisheries Infrastructure Needs Analysis in Order to Capture Fisheries Development Based on Commodities of South Sumatra Province Soaking Period of Cooked Blood Cockle (Anadara granosa) Meat in Alginate Solution to Cadmium Content Reduction Aplikasi Teknologi Aquaponic Pada Budidaya Ikan Air Tawar Untuk Optimalisasi Kapasitas Produksi Production Performance of African Catfish (Clarias gariepinus, burch) were Rearing with Biofloc technology More cited articles
  1. Agustina, P., Sarjito dan A. H. C. Haditomo. 2019. Study of Bacillus methylotrophicus as a Probiotic Candidate Bacteria With Different Concentration Against Aeromonas hydrophila on Water as a Cultivation Media of Tilapia (Oreochromis niloticus). IOP Conf. Series: Earth and Environmental Science, 246: 1-13. https://doi.org/10.1088/1755-1315/246/1/012030
  2. Akinrotimi, O.A., D.O. Bekibele dan O.O. Orokotan. 2011. Select Hematological Values of the African Catfish (Clarias gariepinus) Raised in a Water Recirculating Aquaculture System. International Journal of Recirculating. Aquaculture. 12: 1-12. DOI: http://doi.org/10.21061/ijra.v12i1.1351
  3. Abd Allah, QA., S.M. Aly, H.G.A. El-Rahman, F.M.A. Youssef dan F.K. Ahmed. 2019. Effect of some Immunostimulants on Clinicopathological Findings of African Catfish Clarias gariepinus Infected with Motile Aeromonas Septicemia. Ec Veterinary Science. 4(7): 498-510
  4. Anyanwu M.U, K.F Chah, and VS. Shoyinka, 2015. Evaluation of pathogenicity of motile Aeromonas species in African catfish. Internatinal Journal of Fisheries and Aquatic Studies. 2 (3) : 93 – 98
  5. Ardy, F.M., Desrina dan A.H.C. Haditomo. 2019. Penambahan Kandidat Probiotik Bacillus methylothrophicus Secara Berkala pada Media Pemeliharaan Untuk Pencegahan Infeksi Bakteri Aeromonas hydrophila pada Ikan Nila (Oreochromis niloticus). Jurnal Sains Akuakultur Tropis. 3(2): 50-59. DOI: https://doi.org/10.14710/sat.v3i2.4069
  6. Asniatih, M. Idris dan K. Sabilu. 2013. Studi Histopatologi Ikan Lele Dumbo (Clarias gariepinus) yang Terinfeksi Bakteri Aeromonas hydrophila. Jurnal Mina Laut Indonesia. 3(12): 13-21
  7. Badan Standardisasi Nasional. 2014. Ikan Lele Dumbo (Clarias sp.) Bagian 4: Produksi Benih. SNI 6484.4:2014
  8. Basyuni, M., P.Y.A.B. Ginting dan I. Lesmana. 2017. Phytochemical Analysis of Binahong (Anredera cordifolia) Leaves Extract to Inhibit In Vitro Growth of Aeromonas hydrophila. Proceedings of the 3rd International Symposium on Allied Chemistry pp : 223 -231. https://doi.org/10.1063/1.5011929
  9. Chandrarathna, H.P.S.U., C. Nikapitiya, S.H.S. Dananjaya, C.U.B. Wijerathne, S.H.M.P. Wimalasena, H.J. Kwun, G.J. Heo, J. Lee, dan M.D. Zoysa. 2018. Outcome of Co-infection with Opportunistic and Multidrug Resistant Aeromonas hydrophila and A. veronii in Zebrafish: Identification, Characterization, Pathogenicity and Immune Responses. Fish and Shellfish Immunology. 80: 573-581. https://doi.org/10.1016/j.fsi.2018.06.049Get
  10. Dadiono, M. S., S. Andayani dan K. Zailanie. 2017. The Effect of Different Dosage of Anredera cordifolia (Ten.) Steenis Leaves Extract towards the Survival Rate of African Catfish (Clarias sp.) Infected by Aeromonas salmonicida. International Journal of ChemTech Research,10(4): 669-673
  11. Dageubun, J.L. dan J. Metungun. 2017. Hematology of Vibrio alginolyticus Infected Humpback Grouper Cromileptes altivelis, Under Treatment of Alstonia acuminate Shoot Extract. AACL Bioflux. 10(2): 274-284. http://www.bioflux.com.ro/docs/2017.274-284.pdf
  12. Hardi, E.H., I.W. Kusuma, W. Sueinarti, G. Saptiani, Sumoharjo dan A.M. Lusiastuti. 2017. Utilization of Several Herbal Plant Extracts on Nile Tilapia in Preventing Aeromonas hydrophila and Pseudomonas sp. Bacterial Infection. Nusantara Bioscience. 9(2): 220-228. DOI https://doi.org/10.13057/nusbiosci/n090219
  13. Hardi, E.H., G. Saptiani, I.W. Kusuma, W. Suwinarti dan A. Sudaryono. 2018a. Inhibition of Fish Bacteria Pathogen in Tilapia Using a Concoction Three of Borneo Plant Extracts. IOP Conference Series: Earth and Environmental Science. 144: 1-8. https://doi.org/10.1088/1755-1315/144/1/012015
  14. Hardi, E.H., R.A. Nugroho, G. Saptiani, R. Sabrinah, M. Agriandini dan M. Mawardi. 2018b. Identification of Potentially Pathogenic Bacteria from Tilapia (Oreochromis niloticus) and Channel Catfish (Clarias batracus) Culture in Samarinda, East Kalimantan, Indonesia. Biodiversitas. 19(2): 480-488. DOI https://doi.org/10.13057/biodiv/d190215
  15. Haresmita, P.P. DS. Sakti, N. Yuniarti dan S. Wahyuono. 2019. Phagocytosis Activity of Binahong ,Anredera cordifolia, (Ten.) Steenis from Secang, Magelang, Central Java, Indonesia. Jurnal Farmasi Sains dan Komunitas. 16(1): 7-13. doi: http://dx.doi.org/10.24071/jpsc.001693
  16. KKP, 2018. https://kkp.go.id/djpb/artikel/3042-capaian-kinerja-subsektor-perikanan-budidaya-dan outlook-tahun-2018
  17. Kusdarwati, R., Rozi., D. Dinda dan I. Nurjanah. 2018. Antimicrobial Resistance Prevalence of Aeromonas hydrophila Isolates from Motile Aeromonas septicemia Disease. IOP Conf. Series: Earth and Environmental Science, 137: 1-5. https://doi.org/10.1088/1755-1315/137/1/012076
  18. Lusiastuti, A.M. dan E.H. Hardi. 2018. Gambaran Darah sebagai Indikator Kesehatan pada Ikan Air Tawar. Prosiding Seminar Ikan VI. 65-69
  19. Mawardi, M., A.M. Lusiatuti dan D.F. Idealistina. 2019. Curcuma xanthorrhiza Roxb.: Control Gyrodactyliasis in Catfish Clarias gariepinus. International Journal of Fisheries and Aquatic Studies. 7(6): 197-202
  20. Miladiyah, I. dan B.R. Prabowo. 2012. Ethanolic Extract of Anredera cordifolia (Ten.) Steenis Leaves Improved Wound Healing in Guinea Pigs. Universa Medicina. 31(1): 4-11. DOI: http://dx.doi.org/10.18051/UnivMed.2012.v31.4-11
  21. Muslikha, S. Pujiyanto, S.N. Jannah dan H. Novita. 2016. Isolasi, Karakterisasi Aeromonas hydrophila dan Deteksi Gen Penyebab Penyakit Motile Aeromonas Septicemia (MAS) dengan 16S rRNA dan Aerolysin pada Ikan Lele (Clarias sp.). Jurnal Biologi. 5(4): 1-7
  22. Naibaho, F.F., D. Suryanto dan R. Leidonald. 2018. Jenis-Jenis Bakteri Potensial Patogen pada Ikan Patin (Pangasius sp.) di Kolam Budidaya Ikan Air tawar Kota Beling Tanah Air Kecamatan Tanjung Anom Provinsi Sumatera Utara. Aquacoastmarine. 6(1): 1-11
  23. Putra, G.P., Mulyana dan F.S. Mumpuni. 2015. Pengaruh Pemberian Ekstrak Temulawak (Curcuma xanthoriza Roxb) Terhadap Mortalitas dan Gambaran Darah Benih Ikan Nilem (Osteochilus hasselti) dengan Uji Tantang Menggunakan Bakteri Aeromonas hydrophila. Jurnal Mina Sains. 1(2): 67-78. DOI: http://dx.doi.org/10.30997/jms.v1i2.12
  24. Rahmaningsih, S., M. Zaenuddin dan A. Sudianto. 2018. Gambaran Hematokrit Darah Ikan Lele Sangkuriang (Clarias gariepinus) yang Diberi Pakan Serbuk Daun Majapahit (Cresentia cujete L.) dan Diinfeksi dengan Bakteri Aeromonas hydrophila. Jurnal Kelautan dan Perikanan Terapan. 1(2): 63-67. http://dx.doi.org/10.15578/jkpt.v1i2.7334
  25. Riauwaty, M., W. Windarti, T.E. Y. Sari dan A. Adelina. 2019. Blood Condition of Pangasius hypophthalamus Fed with Tumeric Enriched Pellets and Infected with Aeromonas hydrophila. IOP Conference Series: Earth and Environmental Science. 348: 1-6. https://doi.org/10.1088/1755-1315/430/1/012025
  26. Rosidah, A. Rizal, I. Rustikawati dan F. Octavia. 2018. The Effect of Differences in Altitude Location of an Aquaculture on Fish’s Hematocrit and Fish’s Haemoglobin of Carp Fish and Resistance to Bacterial Attack. IOP Conference Series: Earth and Environmental Science. 137: 1-9. https://doi.org/10.1088/1755-1315/137/1/012008
  27. Rosmawaty, R., Rosidah dan E. Liviawaty. 2016. Pemanfaatan Ekstrak Kulit Jengkol dalam Pakan Ikan Untuk Meningkatkan Imunitas Benih Gurame (Osphronemus gouramy) Terhadap Infeksi Bakteri Aeromonas hydrophila. Jurnal Perikanan dan Kelautan. 7(1): 14-22
  28. Rustikawati, I. 2011. Peningkatan imunitas Ikan Nila (Oreochromis niloticus) terhadap Serangan Streptococciasis Menggunakan Ekstrak Sargassum sp. Indonesian Journal of Apllied Scieces. 1(1): 18-30. https://doi.org/10.24198/.v1i1.1807.g9587
  29. Saparuddin. 2018. Pengaruh Ekstrak Etanol Terhadap Peningkatan Konsentrasi Hemoglobin dan Nilai Hematokrit Ikan Kerapu Tikus. Jurnal Saintifik. 4(1): 39-46. DOI: https://doi.org/10.31605/saintifik.v4i1.142
  30. Sari, N.S., I. Lukistyowati dan N. Aryani. 2012. Pengaruh Pemberian Temulawak (Curcuma xanthorriza Roxb) Terhadap Kelulushidupan Ikan Mas (Cyprinus carpio L) Setelah Diinfeksi Aeromonas hydrophila. Jurnal Perikanan dan Kelautan. 17(2): 43-59
  31. Sarjito, 2010. Aplikasi Biomolekuler dalam Karakterisasi Agensia Penyebab Penyakit Vibrio dan Potensi Bakteri Sponge Sebagai Pengendali Vibriosisi Pada Ikan Kerapu Program Doktor Manajemen Sumberdaya Pantai, Fakultas Pasca Sarjana Universitas Diponegoro
  32. Sarjito., A. H. C. Haditomo., R. W. Ariyati., A. Sabdaningsih., Desrina dan S. B. Prayitno. 2019. Screening of potential isolate candidates probiotic against Aeromonas hydrophila from Boyolali, Indonesia. IOP Conf. Series: Journal of Physics: Conf. Series 1217: 1-7. https://doi.org/10.1088/1742-6596/1217/1/012147
  33. Setiyowati, I., H. Suprapto, dan G. Mahasri. 2019. The Effects of Mercury Chloride (HgCl2) on the Change in Hematology and Blood Sugar Level in Carp (Cyprinus carpio. IOP Conference Series: Earth and Environmental Science. 236: 1-11. https://doi.org/10.1088/1755-1315/236/1/012104
  34. Susanti dan F. Bachmid. 2016. Perbandingan Metode Ekstraksi
  35. Maserasi dan Refluks Terhadap Kadar Fenolik dari
  36. Ekstrak Tongkol Jagung (Zea mays L.). Konversi. (2): 87-93. DOI : https://doi.org/10.24853/konversi.5.2.87-92
  37. Tiamiyu, A.M., Olatoye, I.O. dan Addeji O.B. 2019. Blood Indices of African Catfish (Clarias gariepinus) Following Dietary Administration of Talinum triangulare. Internastional Journal of Research Granthaalayah. 7(4): 185-198. DOI: 10.5281/zenodo.2653843
  38. Wahjuningrum, D., R. Astrini dan M. Setiawati. 2013. Pencegahan Aeromonas hydrophila pada Benih Ikan Lele menggunakan Bawang Putih dan Meniran. Jurnal Akuakultur Indonesia. 12(1): 86-94
  39. Wulansari, F., S.B. Prayitno, dan A.H.C. Haditomo. 2019. Study of Probiotic Candidate CBL20 for Inhibiting of Aeromonas hydrophila With Different Concertration in Tilapia (Oreochromis niloticus). IOP Conference Series: Earth and Environmental Science. 246: 1-8. https://doi.org/10.1088/1755-1315/246/1/012032

Last update:

No citation recorded.

Last update: 2024-12-27 00:41:41

No citation recorded.