DOI: https://doi.org/10.14710/ijfst.17.1.%25p

IDENTIFIKASI POTENSI FOTOPROTEKTIF EKSTRAK RUMPUT LAUT COKELAT Sargassum sp. DENGAN VARIASI PELARUT TERHADAP PAPARAN SINAR ULTRAVIOLET SECARA IN VITRO

*Rarasrum Dyah Kasitowati scopus  -  Fakultas Perikanan dan Ilmu Kelautan-Universitas Brawijaya, Indonesia
Muhammad Miftahul Huda  -  Fakultas Perikanan dan Ilmu Kelautan, Universitas Brawijaya, Indonesia
Rosihan Asmara  -  Fakultas Pertanian, Universitas Brawijaya, Indonesia
Dian Aliviyanti  -  Fakultas Perikanan dan Ilmu Kelautan, Universitas Brawijaya, Indonesia
Feni Iranawati  -  Fakultas Perikanan dan Ilmu Kelautan, Universitas Brawijaya, Indonesia
Mikchaell Alfanov Pardamean Panjaitan  -  Fakultas Perikanan dan Ilmu Kelautan, Universitas Brawijaya, Indonesia
Dwi Candra Pratiwi  -  Fakultas Perikanan dan Ilmu Kelautan, Universitas Brawijaya, Indonesia
Sulastri Arsad  -  Fakultas Perikanan dan Ilmu Kelautan, Universitas Brawijaya, Indonesia

Citation Format:
Abstract

Sargassum sp. dikenal sebagai salah satu sumber hayati yang memiliki kandungan senyawa bioaktif yang berpotensi sebagai bahan esesnsial industri. Namun, komoditi ini kurang bernilai ekonomis sehingga perlu eksplorasi secara optimal untuk meningkatkan nilai ekonomisnya. Salah satu potensi yang dapat dikembangkan adalah aktivitas fotoprotektif yang dimiliki oleh Sargassum sp. Tujuan dari penelitian ini adalah untuk mengeksplorasi aktivitas fotoprotektif Sargassum sp. terhadap paparan sinar UV-B dan UV-A. Potensi fotoprotektif diperoleh dari hasil pengujian nilai SPF (Sun Protection Factor), %Te dan %Tp dari tiga ekstrak Sargassum sp. menggunakan jenir pelarut yang berbeda (methanol, etil asetat dan nheksana). Hasil penelitian menunjukkan bahwa ketiga ekstrak memiliki aktivitas fotoprotektif (SPF) bersifat ultra pada konsentrasi 1100 ppm dan memiliki efek perlindungan (%Te dan %Tp) sebagai Sunblock pada konsentrasi 1100 ppm ekstrak Sargassum sp. Namun dari ketiga ekstrak, ekstrak dengan pelarut etil asetat (ESE) menunjukkan nilai SPF yang tertinggi (32,63) dan nilai %Te (0,055) %Tp (0,075) terendah pada konsentrasi yang sama. Semakin tinggi nilai SPF menunjukkan sifat fotoprotektif yang semakin baik, sedangkan semakin rendah nilai %Te dan %Tp menunjukkan efek perlindungan yang semakin baik.

 

Sargassum sp. known as one of the biological sources that contain bioactive compounds. These compounds have the potential to be industrial essential ingredients. However, this commodity is less economically valuable, so it is important to explore its bioactive compounds in order to increase its economic value. One of the potentials that can be developed is its photoprotective activity. The study aimed  to explore the photoprotective activity of Sargassum sp. against exposure of UV-B and UV-A rays. The photoprotective activity was obtained from the results of the SPF (Sun Protection Factor), % Te and % Tp values of three different Sargassum sp. extracts (methanol, ethyl acetate, and n-hexane). The results showed that the three extracts had an ultra-photoprotective activity (SPF) at a minimum concentration of 1100 ppm and had a protective effect (% Te and% Tp) as sunblocks at the same minimum concentration of 1100 ppm. Among these three extracts, the ethyl acetate extract (ESE) showed the highest SPF value (32.63) and the lowest % Te (0.055) % Tp (0.075) value, at the same concentration. The higher SPF value indicates the better photoprotective properties, while the lower % Te and% Tp values explain the better protective effect.  

Fulltext View|Download
Keywords: pelarut organik; alga; ultraviolet; eritema

Article Metrics:

Article Info
Section: Research Articles
Language : ID
Recent articles
INDEKS KEPEKAAN LINGKUNGAN (IKL) EKOSISTEM LAMUN BERDASARKAN PEMANFAATAN SUMBERDAYA PERIKANAN DI KARIMUNJAWA (Environmental Sensitivity Index (ESI) In Seagrass Ecosystem Based On The Utilization Of Fisheries Resources In Karimunjawa) SOFT CORAL Sinularia gibberosa EXTRACTS ORIGIN PALU BAY, CENTRAL SULAWESI WITH ANTIBACTERIAL AND ANTIOXIDANT ACTIVITY MICROALGAE COMMUNITY AS AQUATIC QUALITY BIOINDICATOR IN PENITI ESTUARY WEST KALIMANTAN More recent articles
Most cited articles
Production Performance of African Catfish (Clarias gariepinus, burch) were Rearing with Biofloc technology Laju Tangkap dan Analisis Usaha Penangkapan Lobster (Panulirus Sp) Dengan Jaring Lobster (Gillnet Monofilament) di Perairan Kabupaten Kebumen PERTUMBUHAN BAKTERI PROBIOTIK Alkaligenus sp. DAN Flavobacterium sp. YANG DIISOLASI DARI USUS UDANG PADA MEDIA KULTUR MOLASE DAN KAOLIN Addition of Phytase Artificial Feed to Increase Digesting, Specific Growth and Survival Rate of Nile Tilapia fingerlings (Oreochromis niloticus) Aplikasi Teknologi Aquaponic Pada Budidaya Ikan Air Tawar Untuk Optimalisasi Kapasitas Produksi More cited articles
  1. Abdassah, M., Aryani, R., Surachman, E., Muchtaridi, M., 2015. In-vitro assessment of effectiveness and photostability avobenzone in cream formulations by combination ethyl ascorbic acid and alpha tocopherol acetate. J. Appl. Pharm. Sci. 5 (6): 70-74. https://doi.org/10.7324/JAPS.2015.50611
  2. Ahn, G.-N., Kim, K.-N., Cha, S.-H., Song, C.-B., Lee, J., Heo, M.-S., Yeo, I.-K., Lee, N.-H., Jee, Y.-H., Kim, J.-S., Heu, M.-S., Jeon, Y.-J., 2007. Antioxidant activities of phlorotannins purified from Ecklonia cava on free radical scavenging using ESR and H2O2-mediated DNA damage. Eur. Food Res. Technol. 226 (1): 71-79. https://doi.org/10.1007/s00217-006-0510-y
  3. Azwanida, N.N., 2015. A review on the extraction methods use in medicinal plants, principle, strength and limitation. Med. Aromat. Plants 4 (3): 196-201. https://doi.org/10.4172/2167-0412.1000196
  4. Bedoux, G., Hardouin, K., Marty, C., Taupin, L., Vandanjon, L., Bourgougnon, N., 2014. Chemical characterization and photoprotective activity measurement of extracts from the red macroalga Solieria chordalis. Bot. Mar. 57 (4): 291-301. https://doi.org/10.1515/bot-2013-0118
  5. Boi, V.N., Cuong, D.X., Khanh Vinh, P.T., 2016. Effects of extraction conditions over the phlorotannin content and antioxidant activity of extract from brown algae Sargassum serratum (Nguyen Huu Dai 2004). Free Radic. Antioxid. 7 (1): 115-122. https://doi.org/10.5530/fra.2017.1.17
  6. Chan, C.-F., Lai, S.-T., Guo, Y.-C., Chen, M.-J., 2014. Inhibitory effects of novel synthetic methimazole derivatives on mushroom tyrosinase and melanogenesis. Bioorg. Med. Chem. 22 (9): 2809-2815. https://doi.org/10.1016/j.bmc.2014.03.009
  7. Chen, L., Hu, J.Y., Wang, S.Q., 2012. The role of antioxidants in photoprotection: A critical review. J. Am. Acad. Dermatol. 67 (5): 1013-1024. https://doi.org/10.1016/j.jaad.2012.02.009
  8. Chang, V.S., Teo, S.S., 2016. Evaluation of heavy metal, antioxidant and anti-tyrosinase activities of red seaweed (Eucheuma cottonii). Int. Food Res. J. 23 (6): 2370-2373
  9. Cha, S.-H., Ko, C.-I., Kim, D., Jeon, Y.-J., 2012. Protective effects of phlorotannins against ultraviolet B radiation in zebrafish (Danio rerio): Ultraviolet B protection in zebrafish. Vet. Dermatol. 23 (1): 51-e12. https://doi.org/10.1111/j.13653164.2011.01009.x
  10. Chan, Y.Y., Kim, K.H., Cheah, S.H., 2011. Inhibitory effects of Sargassum polycystum on tyrosinase activity and melanin formation in B16F10 murine melanoma cells. J. Ethnopharmacol. 137 (3): 1183-1188. https://doi.org/10.1016/j.jep.2011.07.050
  11. Cho, S.H., Cho, J.Y., Kang, S.E., Hong, Y.K., Ahn, D.H., 2008. Antioxidant activity of mojabanchromanol, a novel chromene, isolated from brown alga Sargassum siliquastrum. J. Environ. Biol. 29 (4): 479-484
  12. Cha, S.-H., Ko, C.-I., Kim, D., Jeon, Y.-J., 2012. Protective effects of phlorotannins against ultraviolet B radiation in zebrafish (Danio rerio): Ultraviolet B protection in zebrafish. Vet. Dermatol. 23 (1): 51-e12. https://doi.org/10.1111/j.1365-3164.2011.01009.x
  13. Cikoš, A.-M., Jokić, S., Šubarić, D., Jerković, I., 2018. Overview on the application of modern methods for the extraction of bioactive compounds from marine macroalgae. Mar. Drugs 16 (10): 348-367. https://doi.org/10.3390/md16100348
  14. Cumpelik, B. M. 1972. Analytical Prosedures and Evaluation of Sunscreen. , J.Soc. Cos. Chem
  15. Dewi, M., 2017. Sebaran Kanker di Indonesia, Riset Kesehatan Dasar 2007 11, 8
  16. Fairhead, V.A., Amsler, C.D., McClintock, J.B., Baker, B.J., 2005. Variation in phlorotannin content within two species of brown macroalgae (Desmarestia anceps and D. menziesii) from the Western Antarctic Peninsula. Polar Biol. 28 (9): 680-686. https://doi.org/10.1007/s00300-005-0735-
  17. Fitton, J., Dell’Acqua, G., Gardiner, V.-A., Karpiniec, S., Stringer, D., Davis, E., 2015. Topical benefits of two fucoidan-rich extracts from marine macroalgae. Cosmetics 2 (2): 66-81. https://doi.org/10.3390/cosmetics2020066
  18. Gazali, M., Nurjanah, N., Zamani, N.P., 2018. Eksplorasi senyawa bioaktif alga cokelat Sargassum sp. Agardh sebagai antioksidan dari pesisir barat Aceh. J. Pengolah. Has. Perikan. Indones. 21 (1): 167-178. https://doi.org/10.17844/jphpi.v21i1.21543
  19. Gupta, S., Abu-Ghannam, N., 2011. Bioactive potential and possible health effects of edible brown seaweeds. Trends Food Sci. Technol. 22 (6): 315-326. https://doi.org/10.1016/j.tifs.2011.03.011
  20. Glombitza, K.-W., Keusgen, M., 1995. Fuhalols and deshydroxyfuhalols from the brown alga Sargassum spinuligerum. Phytochemistry 38 (4): 987-995. https://doi.org/10.1016/0031-9422(94)00735-C
  21. Glombitza, K.-W., Keusgen, M., Hauperich, S., 1997. Fucophlorethols from the brown algae Sargassum spinuligerum and Cystophora torulosa. Phytochemistry 46 (8): 1417-1422. https://doi.org/10.1016/S0031-9422(97)00499-
  22. Guinea, M., Franco, V., Araujo-Bazán, L., Rodríguez-Martín, I., González, S., 2012. In vivo UVB-photoprotective activity of extracts from commercial marine macroalgae. Food Chem. Toxicol. 50 (1): 1109-1117. https://doi.org/10.1016/j.fct.2012.01.004
  23. Hönigsmann, H., 2002. Erythema and pigmentation. Photodermatol. Photoimmunol. Photomed. 18 (2): 75-81. https://doi.org/10.1034/j.1600-0781.2002.180204.x
  24. Heo, S.-J., Jeon, Y.-J., 2009. Protective effect of fucoxanthin isolated from Sargassum siliquastrum on UV-B induced cell damage. J. Photochem. Photobiol. B. 95: 101-107. https://doi.org/10.1016/j.jphotobiol.2008.11.011
  25. Hupel, M., Lecointre, C., Meudec, A., Poupart, N., Gall, E.A., 2011. Comparison of photoprotective responses to UV radiation in the brown seaweed Pelvetia canaliculata and the marine angiosperm Salicornia ramosissima. Journal of Experimental Marine Biology and Ecology 401, 36-47
  26. Kim, W.j, Kim, S.M, Kim, H.G, Oh, H.R, Lee, K.B, Park, Y.I. 2007. Purification and Anticoagulant Activity of a Fucoidan from Korean Undaria pinnatifida Sporophyll. ALGAE. 22 (3): 247-252
  27. Lim, S., Choi, A.-H., Kwon, M., Joung, E.-J., Shin, T., Lee, S.-G., Kim, N.-G., Kim, H.-R., 2019. Evaluation of antioxidant activities of various solvent extract from Sargassum serratifolium and its major antioxidant components. Food Chem. 278: 178-184. https://doi.org/10.1016/j.foodchem.2018.11.058
  28. Liu, Y., Liu, M., Zhang, X., Chen, Q., Chen, H., Sun, L., Liu, G., 2016. Protective effect of fucoxanthin isolated from Laminaria japonica against visible light-induced retinal damage both in vitro and in vivo. J. Agric. Food Chem. 64 (2): 416-424. https://doi.org/10.1021/acs.jafc.5b05436
  29. Limantara, L., 2010. Studi komposisi pigmen dan kandungan fukosantin rumput laut cokelat dari perairan Madura dengan kromatografi cair kinerja tinggi. Ilmu Kelaut. 15 (1): 23-32
  30. Li, Y., Fu, X., Duan, D., Liu, X., Xu, J., Gao, X., 2017. Extraction and identification of phlorotannins from the brown alga, Sargassum fusiforme (Harvey) Setchell. Mar. Drugs 15 (2): 49-63. https://doi.org/10.3390/md15020049
  31. Lim, S., Choi, A.-H., Kwon, M., Joung, E.-J., Shin, T., Lee, S.-G., Kim, N.-G., Kim, H.-R., 2019. Evaluation of antioxidant activities of various solvent extract from Sargassum serratifolium and its major antioxidant components. Food Chem. 278: 178-184. https://doi.org/10.1016/j.foodchem.2018.11.058
  32. Michalak, I., Chojnacka, K., 2014. Algal extracts: Technology and advances. Eng. Life Sci. 14 (6): 581-591. https://doi.org/10.1002/elsc.201400139
  33. Maruyama, H., Tamauchi, H., Kawakami, F., Yoshinaga, K., Nakano, T., 2015. Suppressive effect of dietary fucoidan on proinflammatory immune response and MMP-1 expression in UVB-irradiated mouse skin. Planta Med. 81 (15): 1370-1374. https://doi.org/10.1055/s-0035-155782
  34. Mokodompit, A.N., Edy, H.J., Wiyono, W., 2013. Penentuan nilai sun protective factor (SPF) secara in vitro krim tabir surya ekstrak etanol kulit alpukat. J. Ilmiah Farm. 2 (3): 83-85
  35. Matsui, M., Tanaka, Kosuke, Higashiguchi, N., Okawa, H., Yamada, Y., Tanaka, Ken, Taira, S., Aoyama, T., Takanishi, M., Natsume, C., Takakura, Y., Fujita, N., Hashimoto, T., Fujita, T., 2016. Protective and therapeutic effects of fucoxanthin against sunburn caused by UV irradiation. J. Pharmacol. Sci. 132 (1): 55-64. https://doi.org/10.1016/j.jphs.2016.08.004
  36. Mansur, J. de S., Breder, M.N.R., Mansur, M.C.D.A., Azulay, R.D., 1986. Determination of sun protection factor by spectrophotometric methods. An. Bras. Dermatol. 61 (3): 121-124
  37. Maslihah, I., 1987. Studi Aktivitas In Vitro Oktilmetoksinamat dan Oksi Benson serta dalam Bentuk Kombinasinya sebagai Tabir Matahari dalam Satu Basis Krim Nonionik. Universitas Airlangga, Surabaya
  38. Montero, L., Sánchez-Camargo, A.P., García-Cañas, V., Tanniou, A., Stiger-Pouvreau, V., Russo, M., Rastrelli, L., Cifuentes, A., Herrero, M., Ibáñez, E., 2016. Anti-proliferative activity and chemical characterization by comprehensive two-dimensional liquid chromatography coupled to mass spectrometry of phlorotannins from the brown macroalga Sargassum muticum collected on North-Atlantic coasts. J. Chromatogr. A 1428: 115-125. https://doi.org/10.1016/j.chroma.2015.07.053
  39. Namjoyan, F., Farasat, M., Alishahi, M., Jahangiri, A., 2018. The anti-melanogenesis activities of some selected red macroalgae from northern coasts of the Persian Gulf. Iran. J. Pharm. Res. 18 (1): 383-390
  40. Nagappan, H., Pee, P.P., Kee, S.H.Y., Ow, J.T., Yan, S.W., Chew, L.Y., Kong, K.W., 2017. Malaysian brown seaweeds Sargassum siliquosum and Sargassum polycystum : Low density lipoprotein (LDL) oxidation, angiotensin converting enzyme (ACE), α-amylase, and α-glucosidase inhibition activities. Food Res. Int. 99: 950-958. https://doi.org/10.1016/j.foodres.2017.01.023
  41. Nakai, M., Kageyama, N., Nakahara, K., Miki, W., 2006. Phlorotannins as radical scavengers from the extract of Sargassum ringgoldianum. Mar. Biotechnol. 8 (4): 409-414. https://doi.org/10.1007/s10126-005-6168-9
  42. Núñez-Pons, L., Avila, C., Romano, G., Verde, C., Giordano, D., 2018. UV-protective compounds in marine organisms from the Southern Ocean. Mar. Drugs 16 (9): 336-390. https://doi.org/10.3390/md16090336
  43. Nguyen, H., Kim, S.M., 2012. Antioxidative, anticholinesterase and antityrosinase activities of the red alga Grateloupia lancifolia extracts. Afr. J. Biotechnol. 11 (39): 9457-9467. https://doi.org/10.5897/AJB11.2988
  44. Piao, M.J., Kim, K.C., Zheng, J., Yao, C.W., Cha, J.W., Boo, S.J., Yoon, W.J., Kang, H.K., Yoo, E.S., Koh, Y.S., Ko, M.H., Lee, N.H., Hyun, J.W., 2014. The ethyl acetate fraction of Sargassum muticum attenuates ultraviolet B radiation-induced apoptotic cell death via regulation of MAPK- and caspase-dependent signaling pathways in human HaCaT keratinocytes. Pharm. Biol. 52 (9): 1110-1118. https://doi.org/10.3109/13880209.2013.879186
  45. Pereira, L., 2018. Seaweeds as source of bioactive substances and skin care therapy—Cosmeceuticals, algotheraphy, and thalassotherapy. Cosmetics 5 (4): 68-108. https://doi.org/10.3390/cosmetics5040068
  46. Reichardt, C., 2003. Solvents and Solvent Effects in Organic Chemistry, Third. ed. Wiley-VCH, Marburg, Germany
  47. Sedjati, S., Supriyantini, E., Ridlo, A., Soenardjo, N., Santi, V.Y., 2018. Kandungan pigmen, total fenolik dan aktivitas antioksidan Sargassum sp. J. Kelaut. Trop. 21 (2): 137-144. https://doi.org/10.14710/jkt.v21i2.3329
  48. Sayre, R.M., Agin, P.P., LeVee, G.J., Marlowe, E., 1979. A comparison of in vivo and in vitro testing of sunscreening formulas. Photochem. Photobiol. 29 (3): 559-566. https://doi.org/10.1111/j.1751-1097.1979.tb07090.x
  49. Shimoda, H., Tanaka, J., Shan, S.-J., Maoka, T., 2010. Anti-pigmentary activity of fucoxanthin and its influence on skin mRNA expression of melanogenic molecules: Suppressive effect of fucoxanthin on melanin synthesis. J. Pharm. Pharmacol. 62 (9): 1137-1145. https://doi.org/10.1111/j.2042-7158.2010.01139.x
  50. Urikura, I., Sugawara, T., Hirata, T., 2011. Protective effect of fucoxanthin against UVB-induced skin photoaging in hairless mice. Biosci. Biotechnol. Biochem. 75 (4): 757-760. https://doi.org/10.1271/bbb.110040
  51. Wang, H.-M.D., Li, X.-C., Lee, D.-J., Chang, J.-S., 2017. Potential biomedical applications of marine algae. Bioresour. Technol. 244: 1407-1415. https://doi.org/10.1016/j.biortech.2017.05.198
  52. Yanuarti, R., Nurjanah, N., Anwar, E., Pratama, G., 2017. Kandungan senyawa penangkal sinar ultra violet dari ekstrak rumput laut Eucheuma cottonii dan Turbinaria conoides. Biosfera 34 (2): 51-58. https://doi.org/10.20884/1.mib.2017.34.2.467
  53. Zhang, Q.-W., Lin, L.-G., Ye, W.-C., 2018. Techniques for extraction and isolation of natural products: A comprehensive review. Chin. Med. 13 (1): 20-45. https://doi.org/10.1186/s13020-018-0177-x

Last update:

No citation recorded.

Last update: 2024-11-17 09:12:45

No citation recorded.