DOI: https://doi.org/10.14710/ijfst.17.1.%25p

IDENTIFIKASI POTENSI FOTOPROTEKTIF EKSTRAK RUMPUT LAUT COKELAT Sargassum sp. DENGAN VARIASI PELARUT TERHADAP PAPARAN SINAR ULTRAVIOLET SECARA IN VITRO

*Rarasrum Dyah Kasitowati scopus  -  Fakultas Perikanan dan Ilmu Kelautan-Universitas Brawijaya, Indonesia
Muhammad Miftahul Huda  -  Fakultas Perikanan dan Ilmu Kelautan, Universitas Brawijaya, Indonesia
Rosihan Asmara  -  Fakultas Pertanian, Universitas Brawijaya, Indonesia
Dian Aliviyanti  -  Fakultas Perikanan dan Ilmu Kelautan, Universitas Brawijaya, Indonesia
Feni Iranawati  -  Fakultas Perikanan dan Ilmu Kelautan, Universitas Brawijaya, Indonesia
Mikchaell Alfanov Pardamean Panjaitan  -  Fakultas Perikanan dan Ilmu Kelautan, Universitas Brawijaya, Indonesia
Dwi Candra Pratiwi  -  Fakultas Perikanan dan Ilmu Kelautan, Universitas Brawijaya, Indonesia
Sulastri Arsad  -  Fakultas Perikanan dan Ilmu Kelautan, Universitas Brawijaya, Indonesia

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Abstract

Sargassum sp. dikenal sebagai salah satu sumber hayati yang memiliki kandungan senyawa bioaktif yang berpotensi sebagai bahan esesnsial industri. Namun, komoditi ini kurang bernilai ekonomis sehingga perlu eksplorasi secara optimal untuk meningkatkan nilai ekonomisnya. Salah satu potensi yang dapat dikembangkan adalah aktivitas fotoprotektif yang dimiliki oleh Sargassum sp. Tujuan dari penelitian ini adalah untuk mengeksplorasi aktivitas fotoprotektif Sargassum sp. terhadap paparan sinar UV-B dan UV-A. Potensi fotoprotektif diperoleh dari hasil pengujian nilai SPF (Sun Protection Factor), %Te dan %Tp dari tiga ekstrak Sargassum sp. menggunakan jenir pelarut yang berbeda (methanol, etil asetat dan nheksana). Hasil penelitian menunjukkan bahwa ketiga ekstrak memiliki aktivitas fotoprotektif (SPF) bersifat ultra pada konsentrasi 1100 ppm dan memiliki efek perlindungan (%Te dan %Tp) sebagai Sunblock pada konsentrasi 1100 ppm ekstrak Sargassum sp. Namun dari ketiga ekstrak, ekstrak dengan pelarut etil asetat (ESE) menunjukkan nilai SPF yang tertinggi (32,63) dan nilai %Te (0,055) %Tp (0,075) terendah pada konsentrasi yang sama. Semakin tinggi nilai SPF menunjukkan sifat fotoprotektif yang semakin baik, sedangkan semakin rendah nilai %Te dan %Tp menunjukkan efek perlindungan yang semakin baik.

 

Sargassum sp. known as one of the biological sources that contain bioactive compounds. These compounds have the potential to be industrial essential ingredients. However, this commodity is less economically valuable, so it is important to explore its bioactive compounds in order to increase its economic value. One of the potentials that can be developed is its photoprotective activity. The study aimed  to explore the photoprotective activity of Sargassum sp. against exposure of UV-B and UV-A rays. The photoprotective activity was obtained from the results of the SPF (Sun Protection Factor), % Te and % Tp values of three different Sargassum sp. extracts (methanol, ethyl acetate, and n-hexane). The results showed that the three extracts had an ultra-photoprotective activity (SPF) at a minimum concentration of 1100 ppm and had a protective effect (% Te and% Tp) as sunblocks at the same minimum concentration of 1100 ppm. Among these three extracts, the ethyl acetate extract (ESE) showed the highest SPF value (32.63) and the lowest % Te (0.055) % Tp (0.075) value, at the same concentration. The higher SPF value indicates the better photoprotective properties, while the lower % Te and% Tp values explain the better protective effect.  

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Keywords: pelarut organik; alga; ultraviolet; eritema

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