EFECT EKSTRAK KULIT BATANG KELOR (Moringa oleifera Lam) PADA STATUS KESEHATAN DAN KELEULUSHIDUPAN IKAN MAS (Cyprinus carpio) YANG DIINFEKSI Aeromonas hydrophila

*Sarjito Sarjito scopus  -  Diponegoro University, Indonesia
Fifiana Zulaekah  -  sarjito@live.undip.ac.id, Indonesia
Alfabetian Condro Haditomo  -  sarjito@live.undip.ac.id, Indonesia
Desrina Desrina  -  Departemen akuakultur, Indonesia
Restiana Wisnu Ariyati  -  Departemen akuakultur, Indonesia
Slamet Budi Prayitno  -  Departemen akuakultur, Indonesia
Received: 30 Jul 2020; Published: 30 Aug 2020.
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Abstract

Ikan mas banyak dibudidayakan dan memiliki nilai ekonomis penting. Serangan penyakit bercak merah (Motile Aeromonas Septicemia)   masih merupakan kendala dalam budidaya ikan tersebut.  Penyakit ini disebabkan oleh bakteri genus Aeromonas, antara lain  Aeromonas hydrophila.  Untuk mengatasi infeksi bakteri tersebut dimungkinkan untuk menggunakan bahan herbal.  Kulit batang kelor merupakan bahan herbal yang berpotensi sebagai antibakteri.  Tujuan dari penelitian ini adalah mengkaji pengaruh  ekstrak kulit batang kelor terhadap status kesehatan dan kelulushidupan  ikan mas yang diinfeksi  A. hydrophila.   Ikan uji  yang digunakan adalah 120 ekor dengan rata-rata bobot 13,58 ± 2,83 g dan rata - rata panjang 9,93± 0,72 cm yang di infeksi A. hydrophila sebanyak 0,1 mL secara intramuscular dengan kepadatan bakteri 107 CFU/mL. Penelitian ini menggunakan metode eksperimental dengan rancangan acak lengkap 4 perlakuan 3 ulangan. Perlakuan yang diberikan adalah perendaman ekstrak kulit batang kelor dengan konsentrasi 0 mg/L ( perlakuan A), 1000 mg/L (perlakuan B ), 2000 mg/L (perlakuan C ) dan 3000 mg/L (perlakuan D).  Metode perendaman yang digunakan adalah  long bath selama 2 jam.  Perendaman dilakukan setelah gejala klinis dari infeksi A. hydrophila muncul.  Data status kesehatan yang diamati meliputi kelulushidupan, eritrosit, leukosit dan hematokrit. Hasil penelitian menunjukkan bahwa perendaman ekstrak kulit batang kelor berpengaruh nyata terhadap kelulushidupan,  eritrosit, leukosit dan hemoglobin  ikan mas (P<0,05), tetapi tidak berpengaruh pada hematokrit (p>0.05).  Kosentrasi  ekstrak kulit batang kelor  1000 - 3000 mg/L dapat digunakan untuk mengobati ikan mas yang terinfeksi bakteri A. hydrophila. Kelulushidupan tertinggi  83,3± 5,77% dicapai pada perendaman 3000 mg/L. Oleh karena itu perendaman ekstrak kulit batang kelor dengan konsentrasi ini dapat digunakan untuk meningkatkan kelulushidupan  ikan mas yang terinfeksi A.hydrophila.

 

Carp is a  freshwater fish with high economic value that is commonly cultivated. One of the constraints in the cultivation is disease outbreaks cause by Aeromonas hidrophila. Moringa is a plant that has a potential antibacterial agent. Its skin stem can be used as antibacterial agent for Aeromonas hydrophila. This research was aimed to observe the performance of moringa skin stem extract to infected carps according to their survival rate and blood profile. Randomized experimental design was implemented to 120 fishes with average weight 13.59 ± 2.83 g and treated in 4 treatments and 3 replicates. The moringa skin stem extract were A (0 mg / L), B (1000 mg / L), C (2000 mg / L) and D (3000 mg /L) and immersed for 2 hours. Experimental carps were infected with 0.1 mL A. hidrophila at concentration of 107 CFU/mL pour to treatment until appeared clinical sign. The result showed that moringa stem skin extract immersion significantly (P<0.05) improved the survival rate and blood profile, such as leucocytes of experimental carps. The moringa skin stem extract at 1000 mg/L demonstrated the best performance on the survival rate of infected experimental carps (83,3± 5,77%)

 

Keywords: Ikan Mas; kelor; Aeromonas hydrophila; kelulushidupan; profil darah

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Section: Research Articles
Language: ID
In Vol 16, No 2 (2020): SAINTEK PERIKANAN
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  1. Abd Allah, QA., S.M. Aly, H.G.A. El-Rahman, F.M.A. Youssef dan F.K. Ahmed. 2019. Effect of some Immunostimulants on Clinicopathological Findings of African Catfish Clarias gariepinus Infected with Motile Aeromonas Septicemia. Ec Veterinary Science. 4(7): 498-510
  2. Abd El-Gawad, E., A.El Asely, A.M., Soror, E.I., Abbas, A.A., and Austin, B., 2020. Effect of dietary Moringa oleifera leaf on the immune response and control of Aeromonas hydrophila infection in Nile tilapia (Oreochromis niloticus) fry. Aquaculture Interntional., 28: 389–402(2020).. https://link.springer.com/article/10.1007/s10499-019-00469-0
  3. Adelakun KM, Ibrahim AO, Ogialekhe P, Oyelowo OJ, Okunloye AD, 2017. Piscicidal Effect of Moringa oleifera LAM., 1785 (Drustick) on Clarias gariepinus (African Catfish) Juvenile. J Environ Anal Toxicol 7: 512. DOI: 10.4172/2161-0525.1000512
  4. Akinrotimi, O.A., D.O. Bekibele dan O.O. Orokotan. 2011. Select Hematological Values of the African Catfish (Clarias gariepinus) Raised in a Water Recirculating Aquaculture System. International Journal of Recirculating. Aquaculture. 12: 1-12. DOI: http://doi.org/10.21061/ijra.v12i1.1351
  5. Al-Mutairi, M. H., S. Ali., S. M. Aly and Y. Aldebasi. 2016. Antibacterial Activity of Sider (Ziziphus spina-christi), Leaves Extract Against Selected Pathogenic Bacteria. European Journal of Pharmaceutical and Medical Research, 3(5): 138-144
  6. Ayoub, H. F., M. M. E. Tantawy and H. M. R. A. Latief. 2019. Influence of Moringa (Moringa oleifera) and Rosemary (Rosmarinus offcinalis) and Tumeric (curcuma longa) in Immune Parameters and Challenge of Nile Tilapia to Aeromonas hydrophila. Life Science Journal,16(4): 8-15. http://www.lifesciencesite.com/lsj/lsj160419/02_34569lsj160419_8_15.pdf
  7. Badan Standardisasi Nasional. 1999. Produksi Induk Ikan Mas (Cyprinus carpio Linneaus) strain Majalaya kelas induk pokok (Parent Stock SNI : 01- 6131 – 1999
  8. Elabd, H., E. Soror., A. E. Asely., E. A. El-Gawad and A. Abbas. 2019. Dietary Supplementation of Moringa leaf Meal for Freshwater Fish: Effect on Growth and Stress Indices. The Egyptian Journal of Aquatic Research. 45: 265-271. https://doi.org/10.1016/j.ejar.2019.05.009
  9. Eltran, J. M. G., A. T. Mansour., A. S. Alsafuqi., H. M. Ali and M. A. Esteban. 2020. Effects of aqueous and ethanolic leaf extracts from drumstick tree (Moringa oleifera) on gilthead seabream (Sparus aurata L.) leucocytes, and their cytotoxic, antitumor, bactericidal and antioxidant activities. Fish and Shellfish Immunology, 106: 44-55. https://doi.org/10.1016/j.fsi.2020.06.054
  10. Gbadamosi, O.K., Fasakin A., E. and Adebayo, O.T, 2016. Hepatoprotective and stress - reducing effects of dietary Moringa oleifera extract against Aeromonas hydrophila infections and transportation-induced stress in Nile tilapia, Oreochromis niloticus (Linnaeus 1757) fingerlings. International Journal of Environmental & Agriculture Research (IJOEAR) 2 (7) : 121 -128. https://ijoear.com/Paper-July-2016/IJOEAR-JUL-2016-25.pdf
  11. Hammed. A.M., Amosu Af., Awe. Af., and Gbadomosi, F.F. 2015. Effects Of Moringa Oleifera
  12. Infected Adults African Mud Cat Fish (Clarias gariepinus Burchell, 1822). International Journal Of Current Research
  13. (11) : 22117-22122
  14. Harborne, J.B. 1987. Metode Fitokimia Penuntun cara modern menganalisis tumbuhan. ITB, Bandung
  15. Hardi, E.H., I.W. Kusuma, W. Sueinarti, G. Saptiani, Sumoharjo and A.M. Lusiastuti. 2017. Utilization of Several Herbal Plant Extracts on Nile Tilapia in Preventing Aeromonas hydrophila and Pseudomonas sp. Bacterial Infection. Nusantara Bioscience. 9(2): 220-228. DOI https://doi.org/10.13057/nusbiosci/n090219
  16. Hardi, E.H., G. Saptiani, I.W. Kusuma, W. Suwinarti and A. Sudaryono. 2018a. Inhibition of Fish Bacteria Pathogen in Tilapia Using a Concoction Three of Borneo Plant Extracts. IOP Conference Series: Earth and Environmental Science. 144: 1-8. https://doi.org/10.1088/1755-1315/144/1/012015
  17. Hardi, E.H., R.A. Nugroho, G. Saptiani, R. Sabrinah, M. Agriandini and M. Mawardi. 2018b. Identification of Potentially Pathogenic Bacteria from Tilapia (Oreochromis niloticus) and Channel Catfish (Clarias batracus) Culture in Samarinda, East Kalimantan, Indonesia. Biodiversitas. 19(2): 480-488. DOI https://doi.org/10.13057/biodiv/d190215
  18. Ikalinus. R., S.K. Widyastuti, dan N.L.E. Setiasih. 2015. Skrining Fitokimia Ekstrak Etanol Kulit Batang Kelor (Moringa oleifera). Journal Indonesia Medicus Veterinus 4(1) : 71-79
  19. KKP, 2020. https://kkp.go.id/djpb/artikel/3042-capaian-kinerja-subsektor-perikanan-budidaya-dan outlook-tahun-2018
  20. Kaleo, I.V., Q. Gao., B. Liu., C. Sun., Q. Zhou., H. Zhang., F. Shan., Z. Xiong., L. Bo., and C. Song. 2019. Effect of Moringa oleifera leaf Extract on Growth Performance, Physiological and Immune esponse and Related Immune Gene Expression of Freshwater with Aeromonas sp. Fish and Shellfish Immunology, 89: 603-613. https://doi.org/10.1016/j.fsi.2019.03.039
  21. Kavitha, C., M. Ramesh., S. S.Kumaran.,and S. A. Lakshmi. 2017. The Effect of Moringa oleifera Seed Extract on Some Hematological and Biochemical Profiles in a Freshwater Fish, Cyprinus carpio. Experimental Pathology, 64: 681-687. https://doi.org/10.1016/j.etp.2011.01.001
  22. Kong X., Qiao D., Zhao X., Wang L., Zhang J., Liu D., and Zhang H.2017. The molecular characterizations of Cu/ZnSOD and MnSOD and its responses of mRNA expression and enzyme activity to Aeromonas hydrophila or lipopolysaccharide challenge in Qihe crucian carp Carassius auratus. Fish Shellfish Immunol. 67: 429–440. https://doi.org/10.1016/j.fsi.2017.06.031
  23. Leone, A., S. Alberto, B. Alberto, S. Alberto, A. Junior, and B. Simona. 2015. Cultivation, Genetic, Ethnopharmacology, Phytochemistry and Pharmacology of Moringa oleifera Leaves: An Overview. Int. J. Mol. Sci 16 : 12791-12835. doi: 10.3390/ijms160612791
  24. Lusiastuti, A.M. dan E.H. Hardi. 2018. Gambaran Darah sebagai Indikator Kesehatan pada Ikan Air Tawar. Prosiding Seminar Ikan VI. 65-69
  25. Mbokane, EM., and Ngonidzashe., 2018. Alterations of haemato-biochemical parameters pre and post-challenge with Aeromonas hydrophila and survival of Oreochromis mossambicus fed Moringa oleifera-based diets. Fish and shellfish Immunology, 83 : 213 -222. https://doi.org/10.1016/j.fsi.2018.09.017
  26. Muslikha, S. Pujiyanto, S.N. Jannah dan H. Novita. 2016. Isolasi, Karakterisasi Aeromonas hydrophila dan Deteksi Gen Penyebab Penyakit Motile Aeromonas Septicemia (MAS) dengan 16S rRNA dan Aerolysin pada Ikan Lele (Clarias sp.). Jurnal Biologi. 5(4): 1-7
  27. Rahmaningsih, S., M. Zaenuddin dan A. Sudianto. 2018. Gambaran Hematokrit Darah Ikan Lele Sangkuriang (Clarias gariepinus) yang Diberi Perendaman Serbuk Kulit batang Majapahit (Cresentia cujete L.) dan Diinfeksi dengan Bakteri Aeromonas hydrophila. Jurnal Kelautan dan Perikanan Terapan. 1(2): 63-67. DOI: http://dx.doi.org/10.15578/jkpt.v1i2.7334
  28. Rosidah, A. Rizal, I. Rustikawati and F. Octavia. 2018. The Effect of Differences in Altitude Location of an Aquaculture on Fish’s Hematocrit and Fish’s Haemoglobin of Carp Fish and Resistance to Bacterial Attack. IOP Conference Series: Earth and Environmental Science. 137: 1-9 https://doi.org/10.1088/1755-1315/137/1/012008
  29. Rosidah R., Buwono, I.B, Lili W., Suryadi, I.B., and Triandika, A.R., 2019. The Resistance of Sangkuriang Catfish (Clarias gariepinus Burchell 1822) Against Aeromonas hydrophila Bacteria Given Moringa Leaf Extracts (Moringa Oleifera L.) Through the Feed. Jurnal Ikhtyology 19(1) : 97-113 https://doi.10.32491/jii.v19i1.435
  30. Sabzi, E., H. Mohammadiazarm dan A.P. Salati., 2017. Effect of dietary L-carnitine and lipid levels on growth performance, blood biochemical parameters and antioxidant status in juvenile common carp (Cyprinus carpio). Aquaculture, 480: 89-93. https://doi.org/10.1016/j.aquaculture.2017.08.013
  31. Saparuddin. 2018. Pengaruh Ekstrak Etanol Terhadap Peningkatan Konsentrasi Hemoglobin dan Nilai Hematokrit Ikan Kerapu Tikus. Jurnal Saintifik. 4(1): 39-46. DOI: https://doi.org/10.31605/saintifik.v4i1.142
  32. Sarjito, 2010. Aplikasi Biomolekuler dalam Karakterisasi Agensia Penyebab Penyakit Vibrio dan Potensi Bakteri Sponge Sebagai Pengendali Vibriosisi Pada Ikan Kerapu Program Doktor Manajemen Sumberdaya Pantai, Fakultas Paska Sarjana Universitas Diponegoro
  33. Sarjito., A. H. C. Haditomo., R. W. Ariyati., A. Sabdaningsih., Desrina and S. B. Prayitno. 2019. Screening of potential isolate candidates probiotic against Aeromonas hydrophila from Boyolali, Indonesia. IOP Conf. Series: Journal of Physics: Conf. Series 1217: 1-7. https://doi.org/10.1088/1742-6596/1217/1/012147
  34. Sarjito, S., Prayitno, S.B., Rochani, N.Q.S., Haditomo, A.C.H., Amalia, R., dan Desrina., 2020a. Potensi Epibiotik Campuran Ekstrak Daun Binahong (Anredera cordifolia) dan Temulawak (Curcuma zanthorrhiza) Pada Pakan Untuk Mengatasi Infeksi Aeromonas hydrophila Pada Ikan Lele (Clarias gariepinus).Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology, 16(1), 51-58. https://doi.org/10.14710/ijfst.16.1.51-58
  35. Sarjito , S B Prayitno , N T Kusuma and Desrina, 2020b . The potential of mixed epibiotic (binahong leaves, anrederacordifolia, and garlic, allium sativum, extracts) as a feed additive to combat aeromonashydrophilainfection on catfish (clarias gariepinus) . Journal of Physics: Conference Series 1524 (2020) 012063 IOP Publishing. doi: 10.1088/1742-6596/1524/1/012063
  36. Sayeed, M.A., M.S. Hossain, M.E.H. Chowdhury, and M. Haque. 2012. . In vitro Antimicrobial Activity of Methanolic Extract of Moringa oleifera Lam. Fruits. Journal of Pharmacognosy and Phytochemistry. 1(8192):94–95. https://www.phytojournal.com/archives/2012/vol1issue4/PartA/13.1.pdf
  37. Setiyowati, I., H. Suprapto, and G. Mahasri. 2019. The Effects of Mercury Chloride (HgCl2) on the Change in Hematology and Blood Sugar Level in Carp (Cyprinus carpio. IOP Conference Series: Earth and Environmental Science. 236: 1-11. https://doi.org/10.1088/1755-1315/236/1/012104
  38. Sihite, E.R, Rosmaiti, Putriningtias A., dan Putra AS, A., 2020, Pengaruh Padat Tebar Tinggi Terhadap Kualitas Air Dan Pertumbuhan Ikan Mas (Cyprinus Carpio) Dengan Penambahan Nitrobacter. Jurnal Ilmiah Samudra Akuatika Iv(1): 10 -16 .Doi: 10.33059/Jisa.V4i1.2444
  39. Stratev, D., Daskalov H., and I. Vashin, I., 2015. Characterisation and Determination of Antimicrobial Resistance of β-Haemolytic Aeromonas spp. Isolated from Common Carp (Cyprinus carpio L.). Revue Med. Vet, 166(2): 54-61
  40. Stratev D., and Odeyemi O. A. 2016. Antimicrobial resistance of Aeromonas hydrophila isolated from different food sources: A mini-review. J. Infect. Public Health 9: 535–544. htps://doi.org/10.1016/j.jiph.2015.10.006
  41. Tekle, W., N.P. Sahu, and M. Makesh. 2015. Antioxidative and antimicrobial activities of different solvent extracts of Moringa oleifera: an in vitro evaluation. International
  42. Journal of Scientific and Research Publications. 5( 5): 1 – 12.. http://www.ijsrp.org/research-paper-0515.php?rp=P414037Tiamiyu, A.M., Olatoye, I.O. and Addeji O.B. 2019. Blood Indices of African Catfish (Clarias gariepinus) Following Dietary Administration of Talinum triangulare. Internastional Journal of Research Granthaalayah. 7(4): 185-198. DOI: 10.5281/zenodo.2653843
  43. Vallado, G.M.R., Gallani SU, and Pilarski, F., 2015. Phytotherapy as an alternative for treating fish disease. J. Vet. Phar. And Theraupic 38(5): 417 – 428. https://doi.org/10.1111/jvp.12202
  44. Yustiati, A., D. F. Kundari., A. A. H. Suryana, and I. B. B. Suryadi. 2019. Effectiveness of Potassium Diformate Addition to Feed to Improve Immune System of Pangasius (Pangasianodon hypophthalmus) that Challenged by Aeromonas hydrophila. World Scientific News, 134(2): 86-100
  45. Zhao, Z.L., Jin, J.H., Di G.L, Li, L., and Kong, Z.H., 2019.. Molecular characteristics, pathogenicity and medication regimen of Aeromonas hydrophila isolated from common carp (Cyprinus carpio L.) J. Vet. Med. Sci., 81(12): 1769–1775. doi: 10.1292/jvms.19-0025
  46. Zhang, X.,Z. Sun., J. Cai., J. Wang., G. Wang., Z. Zhu., F. Cao. 2020. Effects of Dietary Fish Meal Replacement by Fermented Moringa (Moringa oleifera Lam) Leaves on Growth Performance, Nonspesific Immunity and Disease Resistance Against Aeromonas hydrophila in C.carp. Fish and Shellfish Immunology, 102: 430-439. https://doi.org/10.1016/j.fsi.2020.04.051

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