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EFEK EKSTRAK KULIT BATANG KELOR (Moringa oleifera Lam) PADA STATUS KESEHATAN DAN KELULUSHIDUPAN IKAN MAS (Cyprinus carpio) YANG DIINFEKSI Aeromonas hydrophila

*Sarjito Sarjito scopus  -  Diponegoro University, Indonesia
Fifiana Zulaekah  -  sarjito@live.undip.ac.id, Indonesia
Alfabetian Condro Haditomo  -  sarjito@live.undip.ac.id, Indonesia
Desrina Desrina  -  Departemen akuakultur, Indonesia
Restiana Wisnu Ariyati  -  Departemen akuakultur, Indonesia
Slamet Budi Prayitno  -  Departemen akuakultur, Indonesia

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Abstract

Ikan mas banyak dibudidayakan dan memiliki nilai ekonomis penting. Serangan penyakit bercak merah (Motile Aeromonas Septicemia)   masih merupakan kendala dalam budidaya ikan tersebut.  Penyakit ini disebabkan oleh bakteri genus Aeromonas, antara lain  Aeromonas hydrophila.  Untuk mengatasi infeksi bakteri tersebut dimungkinkan untuk menggunakan bahan herbal.  Kulit batang kelor merupakan bahan herbal yang berpotensi sebagai antibakteri.  Tujuan dari penelitian ini adalah mengkaji pengaruh  ekstrak kulit batang kelor terhadap status kesehatan dan kelulushidupan  ikan mas yang diinfeksi  A. hydrophila.   Ikan uji  yang digunakan adalah 120 ekor dengan rata-rata bobot 13,58 ± 2,83 g dan rata - rata panjang 9,93± 0,72 cm yang di infeksi A. hydrophila sebanyak 0,1 mL secara intramuscular dengan kepadatan bakteri 107 CFU/mL. Penelitian ini menggunakan metode eksperimental dengan rancangan acak lengkap 4 perlakuan 3 ulangan. Perlakuan yang diberikan adalah perendaman ekstrak kulit batang kelor dengan konsentrasi 0 mg/L ( perlakuan A), 1000 mg/L (perlakuan B ), 2000 mg/L (perlakuan C ) dan 3000 mg/L (perlakuan D).  Metode perendaman yang digunakan adalah  long bath selama 2 jam.  Perendaman dilakukan setelah gejala klinis dari infeksi A. hydrophila muncul.  Data status kesehatan yang diamati meliputi kelulushidupan, eritrosit, leukosit dan hematokrit. Hasil penelitian menunjukkan bahwa perendaman ekstrak kulit batang kelor berpengaruh nyata terhadap kelulushidupan,  eritrosit, leukosit dan hemoglobin  ikan mas (P<0,05), tetapi tidak berpengaruh pada hematokrit (p>0.05).  Kosentrasi  ekstrak kulit batang kelor  1000 - 3000 mg/L dapat digunakan untuk mengobati ikan mas yang terinfeksi bakteri A. hydrophila. Kelulushidupan tertinggi  83,3± 5,77% dicapai pada perendaman 3000 mg/L. Oleh karena itu perendaman ekstrak kulit batang kelor dengan konsentrasi ini dapat digunakan untuk meningkatkan kelulushidupan  ikan mas yang terinfeksi A.hydrophila.

 

Carp is a  freshwater fish with high economic value that is commonly cultivated. One of the constraints in the cultivation is disease outbreaks cause by Aeromonas hidrophila. Moringa is a plant that has a potential antibacterial agent. Its skin stem can be used as antibacterial agent for Aeromonas hydrophila. This research was aimed to observe the performance of moringa skin stem extract to infected carps according to their survival rate and blood profile. Randomized experimental design was implemented to 120 fishes with average weight 13.59 ± 2.83 g and treated in 4 treatments and 3 replicates. The moringa skin stem extract were A (0 mg / L), B (1000 mg / L), C (2000 mg / L) and D (3000 mg /L) and immersed for 2 hours. Experimental carps were infected with 0.1 mL A. hidrophila at concentration of 107 CFU/mL pour to treatment until appeared clinical sign. The result showed that moringa stem skin extract immersion significantly (P<0.05) improved the survival rate and blood profile, such as leucocytes of experimental carps. The moringa skin stem extract at 1000 mg/L demonstrated the best performance on the survival rate of infected experimental carps (83,3± 5,77%)

 

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Keywords: Ikan Mas; kelor; Aeromonas hydrophila; kelulushidupan; profil darah

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