Isolasi, Identifikasi dan Uji Aktivitas Antibakteri Minyak Atsiri Akar Sidaguri (Sida rhombifolia Linn)

Winarti Winarti; Dewi Kusrini; Enny Fachriyah

doi:10.14710/jksa.12.2.52-56

DOI: https://doi.org/10.14710/jksa.12.2.52-56

Isolasi, Identifikasi dan Uji Aktivitas Antibakteri Minyak Atsiri Akar Sidaguri (Sida rhombifolia Linn)

Winarti Winarti, Dewi Kusrini

, Enny Fachriyah

Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University, Indonesia

Published: 1 Aug 2009.

Citation Format:

Abstract

Telah dilakukan penelitian tentang isolasi, identifikasi dan uji aktivitas antibakteri minyak atsiri akar sidaguri (Sida rhombifolia Linn). Penelitian ini bertujuan untuk mengisolasi, mengidentifikasi senyawa minyak atsiri dari akar sidaguri serta mengetahui aktivitasnya sebagai antibakteri. Metode yang dilakukan dalam penelitian ini meliputi destilasi uap, brine shrimp lethality test (BSLT), Kromatografi Gas-Spektrometer Massa (GC-MS) dan metode difusi cakram kertas. Dari hasil penelitian diperoleh minyak berwarna kuning muda berbentuk seperti pasta yang berbau khas sidaguri dengan rendemen 0,024 % dan indeks bias sebesar 1,569 pada 25°C. Analisis Kromatografi Gas-Spektrometer Massa (GC-MS) menunjukkan bahwa minyak atsiri akar sidaguri didominasi oleh 2 senyawa: 4-terpineol dan α-terpineol. Hasil uji aktivitas minyak atsiri terhadap bakteri Escherichia coli pada konsentrasi 750 ppm dan 1000 ppm menunjukkan luas daerah hambatan sebesar 0,0176 mm2 dan 0,0197 mm2, sedangkan minyak atsiri pada bakteri Staphylococcus aureus sebesar 0,0123 mm2 dan 0,0158 mm2.

Fulltext View|Download

Keywords: antibakteri; minyak atsiri; Sida rhombifolia Linn.

Article Metrics:

Article Info

Section: Research Articles

Language : ID

In Vol 12, No 2 (2009): Volume 12 Issue 2 Year 2009

Recent articles

Efek NaCl dan Anode Besi terhadap Rendemen Mg(OH)2 pada Elektrolisis Sistem Fe|MgSO4,NaCl||H2O|C Pengolahan Limbah Cair Industri Batik dengan Metoda Elektrokoagulasi Menggunakan Seng Bekas Sebagai Elektroda Isolasi, Identifikasi dan Uji Aktivitas Antibakteri Minyak Atsiri Akar Sidaguri (Sida rhombifolia Linn) More recent articles

Most cited articles

Molecular Docking of Interaction between E-Cadherin Protein and Conformational Structure of Cyclic Peptide ADTC3 (Ac-CADTPC-NH2) Simulated on 20 ns New Chemicals and Routes for the Preparation of Gelatin/HA Composites using the Wet Precipitation Method Activity Test of Suji Leaf Extract (Dracaena angustifolia Roxb.) on in vitro cholesterol lowering Dimerisasi Metilisoeugenol dengan Katalis HCl Pengaruh Pemerangkapan Enzim Alkalin Fosfatase ke dalam Silika dari Abu Sekam Padi terhadap Aktivitas Enzimatiknya More cited articles

Yuharmen, Yum Eryanti, Nurbalatif, Uji Aktivitas Antimikroba Minyak Atsiri dan Ekstrak Metanol Lengkuas (Alpinia galanga), in, Jurusan Kimia, FMIPA, Universitas Riau, 2002
P Aureli, A Costantinf, S Zolea, Antimicrobial activity of some plant essential oils against Listeria monocytogenes, Journal of Food Protection®, 55 (1992) 344-348
Bulan Rumondang, Esterifikasi Patchouli Alkohol Hasil Isolasi Dari Minyak Daun Nilam (Patchoulli Oil), in, Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Sumatera Utara, Medan, 2004
Arief Hariana, 262 Tumbuhan Obat dan Khasiatnya, Penebar Swadaya, Jakarta, 2013
SA Dahanukar, RA Kulkarni, NN Rege, Pharmacology of medicinal plants and natural products, Indian Journal of Pharmacology, 32 (2000) 81-118
Md. Ekramul Islam, Naznin Ara Khatune, M. I. I. Wahed, Md. Ekramul Haque, Md. Ashiik Mosaddik Mosaddik, Larvicidal Activity of a New Glycoside, Phenyl Ethyl β-D Glucopyranoside from the Stem of the Plant Sida rhombifolia, Pakistan Journal of Biological Sciences, 6 (2003) 73-75
Faticha Dewi Andarini, Dewi Kusrini, Purbowatiningrum Ria Sarjono, Isolasi, Identifikasi dan Uji Aktivitas Antibakteri Alkaloid Total dari Daun Sidaguri (Sida rhombifolia Linn), in: Jurusan Kimia, Universitas Diponegoro, Semarang, 2009
Ferawati Kusuma, Dewi Kusrini, Enny Fachriyah, Isolasi, Identifikasi, dan Uji Toksisitas Minyak Atsiri Daun Sidaguri (Sida rhombifolia Linn), in: Jurusan Kimia, Universitas Diponegoro, Semarang, 2009
Michael J Pelczar, ECS Chan, Dasar-dasar mikrobiologi, Universitas Indonesia Press, Jakarta, 1988
M Hudayanti, Aktivitas Antibakteri Rimpang Temulawak (Curcuma xanthorrihza Roxb.), in: Jurusan Kimia, Institut Pertanian Bogor, Bogor, 2004
Moghis U Ahmad, SK Husain, M Ahmad, SM Osman, R Subbarao, Cyclopropenoid fatty acids in seed oils ofSida acuta andSida rhombifolia (malvaceae), Journal of the American Oil Chemists’ Society, 53 (1976) 698-699
BN Meyer, NR Ferrigni, JE Putnam, LB Jacobsen, DE j Nichols, Jerry L McLaughlin, Brine shrimp: a convenient general bioassay for active plant constituents, Planta medica, 45 (1982) 31-34
W Bibiana, S Hastowo, Mikrobiologi, Rajawali Pers, Jakarta, 1992
Usman Suwandi, Mekanisme Kerja Antibiotik, Cermin Dunia Kedokteran, (1992)

Last update:

Mechanism of ethyl acetate extract (isolate TE 325) against Escherichia coli with atomic absorption spectrophotometry and UV-Vis spectrophotometry analysis
Alfian Syarifuddin, Ni Made Ayu Nila Septianingrum, Nanik Sulistyani. 3RD INTERNATIONAL CONFERENCE OF BIO-BASED ECONOMY FOR APPLICATION AND UTILITY, 2730 , 2023. doi: 10.1063/5.0120528

Last update: 2024-04-20 13:52:57

No citation recorded.

As an article writer, the author has the right to use their articles for various purposes, including use by institutions that employ authors or institutions that provide funding for research. Author rights are granted without special permission.

Author who publishes a paper at JKSA has the broad right to use their work for teaching and scientific purposes without the need to ask permission, including: used for (i) teaching in the author's class or institution, (ii) presentation at meetings or conferences and distributing copies to participants ; (iii) training conducted by the author or author's institution; (iv) distribution to colleagues for research use; (v) use in the compilation of subsequent authors' works; (vi) inclusion in a thesis or dissertation; (vi) reuse of part of the article in another work (with citation); (vii) preparation of derivative works (with citation); (viii) voluntary posting on open websites operated by authors or author institutions for scientific purposes (follow the CC BY-SA License).

Authors and readers can copy and redistribute material in any media or format, and mix, modify, and build material for any purpose but they must provide appropriate credit (provide article citation or content), providing links to the license, and indicate if there are changes.

The authors submitting a manuscript do so on the understanding that if accepted for publication, copyright of the article shall be assigned to Jurnal Kimia Sains dan Aplikasi (JKSA). Copyright encompasses rights to reproduce and deliver the article in all form and media, including reprints, photographs, microfilms and any other similar reproductions, as well as translations.

Reproduce any part of this journal, its storage in the database or its transmission by all forms or media is permitted does not need for written permission from JKSA. However, it should be cited as an honor in academic manners

JKSA and the Chemistry Department of Diponegoro University and the Editor make every effort to ensure that there are no data, opinions, or false or misleading statements published in JKSA. However, the content of the article is the sole and exclusive responsibility of each author.

The Copyright Transfer Form can be downloaded here: [Copyright Transfer Form - Indonesian] [Copyright Transfer Form - English]. The copyright form should be signed originally and send to the Editor in the form of printed letters, scanned documents sent via email or fax.

Adi Darmawan, Ph.D (Editor in Chief)

Editor in chief of Jurnal Kimia Sains dan Aplikasi (JKSA)

Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Jl. Prof. Soedarto, Kampus Undip Tembalang, Semarang, Central Java, Indonesia 50275
Email: jksa@live.undip.ac.id