Pengaruh Garam Monovalen (NaCl dan KCl) dan Divalen (CaCl2 dan MgCl2) Terhadap Aktivitas Protease Ekstraseluler Bakteri Halofilik Isolat Bittern Tambak Garam Madura

Siti Anisa  -  Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University, Indonesia
Nies Suci Mulyani  -  Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University, Indonesia
*Mukhammad Asy’ari orcid scopus  -  Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University, Indonesia
Published: 1 Apr 2017.
Open Access Copyright 2017 Jurnal Kimia Sains dan Aplikasi
License URL: http://creativecommons.org/licenses/by-sa/4.0/

Citation Format:
Abstract
Bakteri halofilik mampu tumbuh dan bertahan hidup pada lingkungan dengan kadar garam tinggi, seperti dalam air pekatan sisa tambak garam (bittern). Bakteri halofilik dapat menghasilkan enzim protease yang tahan terhadap garam (halostabil). Protease halostabil dapat dimanfaatkan pada proses degradasi limbah pada kawasan yang mengandung garam. Penelitian ini bertujuan untuk memperoleh protease ekstraseluler bakteri halofilik isolat bittern tambak garam Madura dan menentukan pengaruh garam monovalen (NaCl dan KCl) dan divalen (CaCl2 dan MgCl2) terhadap aktivitas dan profil aktivitas protease halostabil. Berdasarkan penelitian diperoleh protease halostabil memiliki aktivitas spesifik tertinggi pada fraksi 1 (0-20%) sebesar 220,83 Unit/mg protein. Pengaruh garam NaCl, CaCl2 dan MgCl2 akan cenderung meningkatkan aktivitas proteasenya sebaliknya KCl akan menurunkan aktivitas protease. Profil pengaruh penambahan garam terhadap aktivitas protease semakin meningkat sesuai dengan urutan KCl. NaCl, CaCl2, MgCl2.
Keywords: bakteri halofilik; protease halostabil; bittern; garam monovalen; divalen

Article Metrics:

Article Info
Section: Research Articles
Language: ID
In Vol 20, No 1 (2017): Volume 20 Issue 1 Year 2017
Statistics: 3360 2118
Related articles
Studi Filogeni dan Uji Potensi Antibakteri serta Enzim Ekstraseluler Isolat Geobacillus sp. dari Sumber Air Panas Gedongsongo Konsentrasi Hambat Minimum (KHM) Kadar Sampel Alang-Alang (Imperata cylindrica) dalam Etanol Melalui Metode Difusi Cakram Antibacterial Activity and Mechanism of Action of Methanol Extract from Kasturi Mango Fruit (Mangifera casturi) on Caries-Causing Bacterium Streptococcus mutans Isolasi Bakteri Termofilik Sumber Air Panas Gedongsongo dengan Media Pengaya MB (Minimal Broth) dan TS (Taoge Sukrosa) serta Identifikasi Fenotip dan Genotip Pengaruh Konsentrasi Substrat Maltosa terhadap Potensial Listrik Baterai Lactobacillus bulgaricus (MFC) DNA Polimerase sebagai Model Kajian Biomolekul Enzim Termostabil Isolat Lokal
  1. Aharon Oren, Halophilic Microorganisms and their Environments, Springer Science & Business Media, 2002
  2. Shiladitya DasSarma, Priya DasSarma, Halophiles, in: Encyclopedia of Life Science, Wiley, 2006
  3. Mital S. Dodia, Rupal H. Joshi, Rajesh K. Patel, Satya P. Singh, Characterization and stability of extracellular alkaline proteases from halophilic and alkaliphilic bacteria isolated from saline habitat of coastal Gujarat, India, Brazilian Journal of Microbiology, 37, (2006) 276-282 http://dx.doi.org/10.1590/S1517-83822006000300015
  4. Neil D. Rawlings, Protease Families, Evolution and Mechanism of Action, in: K. Brix, W. Stöcker (Eds.) Proteases: Structure and Function, Springer Vienna, Vienna, 2013, pp. 1-36
  5. P. Norberg, B. V. Hofsten, Proteolytic Enzymes from Extremely Halophilic Bacteria, Microbiology, 55, 2, (1969) 251-256 http://dx.doi.org/10.1099/00221287-55-2-251
  6. Janos K. Lanyi, Salt-dependent properties of proteins from extremely halophilic bacteria, Bacteriological Reviews, 38, 3, (1974) 272-290
  7. Janos K. Lanyi, Joann Stevenson, Studies of the Electron Transport Chain of Extremely Halophilic Bacteria: IV. Role of Hydrophobic Forces in The Structure of Menadione Reductase, Journal of Biological Chemistry, 245, 16, (1970) 4074-4080
  8. Malashetty Vidyasagar, S Prakash, Carol Litchfield, K Sreeramulu, Purification and characterization of a thermostable, haloalkaliphilic extracellular serine protease from the extreme halophilic archaeon Halogeometricum borinquense strain TSS101, Archaea, 2, 1, (2006) 51-57
  9. Ronald M. Atlas, Richard Bartha, Microbial Ecology: Fundamentals and Applications, 3 ed., Benjamin/Cummings Publishing Company, 1993
  10. John P. Harley, Lansing M. Prescott, Laboratory Exercises in Microbiology, 5 ed., McGraw-Hill, 2001
  11. Werasit Kanlayakrit, Preeyanuch Bovornreungroj, Takuji Oka, Masatoshi Goto, Production and characterization of protease from an extremely halophilic Halobacterium sp. PB407, Kasetsart Journal: Natural Science, 38, 5, (2004) 15-20
  12. J. Charney, R. M. Tomarelli, A colonmetric method for the determination of the proteolytic activity of duodenal juice, Journal of Biological Chemistry, 171, (1947) 501-505
  13. Terry J. Beveridge, Structures of Gram-Negative Cell Walls and Their Derived Membrane Vesicles, Journal of Bacteriology, 181, 16, (1999) 4725-4733
  14. R. McClelland, Gram's stain: the key to microbiology, MLO Med Lab Obs, 33, 4, (2001) 20-22, 25-28; quiz 30-21
  15. Clive Dennison, A Guide to Protein Isolation, Kluwer Academic Publishers, 1999
  16. Tim Bugg, Introduction to Enzyme and Coenzyme Chemistry, John Wiley & Sons, 2004

Last update: 2021-04-22 16:39:42

No citation recorded.

Last update: 2021-04-22 16:39:42

No citation recorded.
Copyright 2017 Jurnal Kimia Sains dan Aplikasi
License URL: http://creativecommons.org/licenses/by-sa/4.0/

As an article writer, the author has the right to use their articles for various purposes, including use by institutions that employ authors or institutions that provide funding for research. Author rights are granted without special permission.

Author who publishes a paper at JKSA has the broad right to use their work for teaching and scientific purposes without the need to ask permission, including: used for (i) teaching in the author's class or institution, (ii) presentation at meetings or conferences and distributing copies to participants ; (iii) training conducted by the author or author's institution; (iv) distribution to colleagues for research use; (v) use in the compilation of subsequent authors' works; (vi) inclusion in a thesis or dissertation; (vi) reuse of part of the article in another work (with citation); (vii) preparation of derivative works (with citation); (viii) voluntary posting on open websites operated by authors or author institutions for scientific purposes (follow the CC BY-SA License).

Authors and readers can copy and redistribute material in any media or format, and mix, modify, and build material for any purpose but they must provide appropriate credit (provide article citation or content), providing links to the license, and indicate if there are changes.

The authors submitting a manuscript do so on the understanding that if accepted for publication, copyright of the article shall be assigned to Jurnal Kimia Sains dan Aplikasi (JKSA). Copyright encompasses rights to reproduce and deliver the article in all form and media, including reprints, photographs, microfilms and any other similar reproductions, as well as translations.

Reproduce any part of this journal, its storage in the database or its transmission by all forms or media is permitted does not need for written permission from JKSA. However, it should be cited as an honor in academic manners

JKSA and the Chemistry Department of Diponegoro University and the Editor make every effort to ensure that there are no data, opinions, or false or misleading statements published in JKSA. However, the content of the article is the sole and exclusive responsibility of each author.

The Copyright Transfer Form can be downloaded here: [Copyright Transfer Form - Indonesian] [Copyright Transfer Form - English]. The copyright form should be signed originally and send to the Editor in the form of printed letters, scanned documents sent via email or fax.

 

Adi Darmawan, Ph.D (Editor in Chief)

Editor in chief of Jurnal Kimia Sains dan Aplikasi (JKSA)

Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Jl. Prof. Soedarto, Kampus Undip Tembalang, Semarang, Central Java, Indonesia 50275
Email: jksa@live.undip.ac.id