Encapsulation of Alkaline Phosphatase in Mesoporous Methyl-Silica Hybrid by Sol-Gel Process

Sriyanti Sriyanti

doi:10.14710/jksa.20.3.110-113

DOI: https://doi.org/10.14710/jksa.20.3.110-113

Encapsulation of Alkaline Phosphatase in Mesoporous Methyl-Silica Hybrid by Sol-Gel Process

Sriyanti Sriyanti

Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University, Indonesia

Published: 1 Oct 2017.

BibTex Citation Data :

@article{JKSA16716,
    author = {Sriyanti Sriyanti},
    title = {Encapsulation of Alkaline Phosphatase in Mesoporous Methyl-Silica Hybrid by Sol-Gel Process},
    journal = {Jurnal Kimia Sains dan Aplikasi},
  volume = {20},
    number = {3},
    year = {2017},
    keywords = {encapsulation; alkaline phosphatase; methyl-silica hybrid},
    abstract = {In recent years, the sol-gel technique has attracted increasing interest as a unique approach to immobilize biomolecules for bioanalitical applications as well as biochemical and biophysical studies. In this research, encapsulation of Alkaline Phosphatase (ALP) enzyme in mesoporous methyl-silica hybrid by sol-gel process has been carried out. Mesoporous methyl-silica hybrid has been synthesis by using tetraethylorthosilicate (TEOS) and methyltriethoxysilane (MTES) as precursor and poly(ethylene) glycol (PEG) as a polymer dopant. The preparation of methyl-silica hybrid was carried out at various mol ratios of precursors and PEG concentration. Encapsulation was emphasized by mixing sol solution and tris-HCl buffer containing ALP. The Surface Area Analyzer analysis data showed that the pore diameter and surface area of methyl-silica hybrid tended to increase as the PEG dopant concentration increases. Result showed that ALP encapsulated in the methyl-silica hybrids with PEG dopant gave enzymatic activity as 0.112 U/mg enzyme, higher than ALP encapsulated in copolymerization of TEOS with MTES or in silica from TEOS precursor only (0.098 U/mg enzyme and 0.069 U/mg enzyme, respectively). It was shown that copolymerization of TEOS with organosilane precursor and the entrapment of polymer additives into sol-gel processed materials are useful methods for modifying the internal environment and hence the activity of biomolecules entrapped in sol-gel derived biomaterials.},
   issn = {2597-9914},   pages = {110--113}  doi = {10.14710/jksa.20.3.110-113},
    url = {https://ejournal.undip.ac.id/index.php/ksa/article/view/16716}
}

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Abstract

In recent years, the sol-gel technique has attracted increasing interest as a unique approach to immobilize biomolecules for bioanalitical applications as well as biochemical and biophysical studies. In this research, encapsulation of Alkaline Phosphatase (ALP) enzyme in mesoporous methyl-silica hybrid by sol-gel process has been carried out. Mesoporous methyl-silica hybrid has been synthesis by using tetraethylorthosilicate (TEOS) and methyltriethoxysilane (MTES) as precursor and poly(ethylene) glycol (PEG) as a polymer dopant. The preparation of methyl-silica hybrid was carried out at various mol ratios of precursors and PEG concentration. Encapsulation was emphasized by mixing sol solution and tris-HCl buffer containing ALP. The Surface Area Analyzer analysis data showed that the pore diameter and surface area of methyl-silica hybrid tended to increase as the PEG dopant concentration increases. Result showed that ALP encapsulated in the methyl-silica hybrids with PEG dopant gave enzymatic activity as 0.112 U/mg enzyme, higher than ALP encapsulated in copolymerization of TEOS with MTES or in silica from TEOS precursor only (0.098 U/mg enzyme and 0.069 U/mg enzyme, respectively). It was shown that copolymerization of TEOS with organosilane precursor and the entrapment of polymer additives into sol-gel processed materials are useful methods for modifying the internal environment and hence the activity of biomolecules entrapped in sol-gel derived biomaterials.

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Keywords: encapsulation; alkaline phosphatase; methyl-silica hybrid

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Section: Research Articles

Language : EN

In Vol 20, No 3 (2017): Volume 20 Issue 3 Year 2017

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Bakul C. Dave, Bruce Dunn, Joan Selverstone Valentine, Jeffrey I. Zink, Sol-gel encapsulation methods for biosensors, Analytical Chemistry, 66, 22, (1994) 1120A-1127A http://dx.doi.org/10.1021/ac00094a001
Wen Jin, John D. Brennan, Properties and applications of proteins encapsulated within sol–gel derived materials, Analytica Chimica Acta, 461, 1, (2002) 1-36 https://doi.org/10.1016/S0003-2670(02)00229-5
Yen Wei, Jigeng Xu, Qiuwei Feng, Hua Dong, Muduo Lin, Encapsulation of enzymes in mesoporous host materials via the nonsurfactant-templated sol–gel process, Materials Letters, 44, 1, (2000) 6-11 http://dx.doi.org/10.1016/S0167-577X(99)00287-6
Tracey Keeling-Tucker, Michael Rakic, Cassandra Spong, John D. Brennan, Controlling the Material Properties and Biological Activity of Lipase within Sol−Gel Derived Bioglasses via Organosilane and Polymer Doping, Chemistry of Materials, 12, 12, (2000) 3695-3704 http://dx.doi.org/10.1021/cm000491m

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